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    • 29. 发明专利
    • Shrimp alkaline phosphatase
    • AU9398701A
    • 2002-04-22
    • AU9398701
    • 2001-10-10
    • NORWEGIAN INST OF FISHERIES ANGARDNER REBECCA
    • GARDNER REBECCANILSEN INGEOVERBO KERSTI
    • A01K67/027A01K67/033C12N1/15C12N1/19C12N1/21C12N5/10C12N9/16C12N15/09C12N15/52
    • The present invention relates to a nucleic acid molecule comprising a nucleotide sequence as set forth in SEQ ID Nos. 1 or 20 or having at least 55% sequence identify therewith and/or capable of hybridising under medium stringency conditions to the complementary sequence of SEQ ID Nos. 1 or 20, or the complementary sequence of said sequences, wherein said nucleotide sequence encodes or is complementary to a sequence which encodes a heat labile alkaline phosphatase and to a recombinant heat labile alkaline comprising: (a) all or a significant part of an amino acid sequence as shown in SEQ ID No. 2; or (b) all or a significant part of an amino acid sequence which has at least 60% sequence identify with SEQ ID No. 2 as well as methods for the manufacture thereof. 1 k AYWNKDAQDALDKQLGIKLREKQAKNVIFFLGDGMSLSTVTAARIYKGG 5ReRP6:17 51 LTGKFEREKISWEEFDFAALSKTYNTDKQ AYLTGVKTNQGV Active site 101 IGLDANTVRTNCSYQLDESLFTYSIAHWFQEAGRSTGVVTSTRVTHATPA 151 GTYAHVADRDWENDSDVVHDREDPEICDDIAEQLVFREPGKNFK VIMGGG 5ReRP6:26 201 RRGFFPEEALDIEDGIPGEREDGK HLITDWLDDKASQGATASYVWNRDDL H23:30 251 LAVDIRNTDYLMGLFSYTHLDTVLTRDAEMDPTLPEMTKVAIEMLTKDEN 301 GFFLLVEGGRIDHMHHANQIRQSLAETLDMEEAVSMALSMTDPEETIILV 351 TADHGHTLTITGYADRNTDILDFAGISDLDDRRYTILDYGSGPGYHITED 401 GKRYEPTEEDLK DINFRYASAAPKHSVTHDGTDVGIWVNGPFAHLFTGVY H23:18 451 EENYIPHALAYAACVGTGRTFCDEK *