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21. 发明授权

US5547936A Inhibition of cell migration with synthetic peptides 失效
标题翻译：用合成肽抑制细胞迁移
公开(公告)号：US5547936A
公开(公告)日：1996-08-20
申请号：US169743
申请日：1993-12-17
申请人： Erkki I. Ruoslahti , Michael D. Pierschbacher , Kurt R. Gehlsen
发明人： Erkki I. Ruoslahti , Michael D. Pierschbacher , Kurt R. Gehlsen
IPC分类号： A61K38/07 , A61K38/08 , A61L27/22 , C07K14/78 , A61K38/04 , A61K38/16
CPC分类号： C07K14/78 , A61K38/07 , A61K38/08 , A61L27/227
摘要： A method of inhibiting the invasion of cells, particularly malignant cells through an extracellular membrane by contacting the membrane-cell interface with synthetic Arg-Gly-Asp-containing peptides. In one embodiment, the invention provides peptides containing the amino acid sequence Arg-Gly-Asp-Thr, more specifically Gly-Arg-Gly-Asp-Thr-Pro, which inhibits the attachment of cells to type I collagen in addition to fibronectin and vitronectin, and a method of inhibiting the attachment of cells to type I collagen. The invention further provides an assay for quantitating the invasive quality of cells by determining the amount of such peptides necessary to prevent the cells from penetrating an extracellular membrane, such as an amniotic membrane, in vitro.
摘要翻译：通过使膜 - 细胞界面与合成的含有Arg-Gly-Asp的合成肽接触来抑制细胞侵入的方法，特别是恶性细胞通过细胞外膜。在一个实施方案中，本发明提供了含有氨基酸序列Arg-Gly-Asp-Thr，更特别地Gly-Arg-Gly-Asp-Thr-Pro的肽，其除了纤连蛋白以外还抑制细胞与I型胶原的连接，玻连蛋白，以及抑制细胞对I型胶原的附着的方法。本发明进一步提供了一种用于通过确定在体外测定防止细胞穿透细胞外膜（例如羊膜）所必需的这种肽的量来量化细胞侵入性质量的测定法。

22. 发明授权

US5453489A Polypeptide fragments of fibronectin which can modulate extracellular matrix assembly 失效
标题翻译：纤连蛋白的多肽片段可以调节细胞外基质组装
公开(公告)号：US5453489A
公开(公告)日：1995-09-26
申请号：US829462
申请日：1992-01-31
申请人： Erkki I. Ruoslahti , Alex Morla
发明人： Erkki I. Ruoslahti , Alex Morla
IPC分类号： C12N15/09 , A61K38/00 , A61P35/00 , A61P43/00 , C07K14/78 , C12N15/12 , C12P21/02 , C12R1/01 , C07K14/00
CPC分类号： C07K14/78 , A61K38/00
摘要： In accordance with the present invention, there is provided a novel synthetic polypeptide derived from the first type III repeat of fibronectin. The synthetic polypeptide of the invention encompasses a fibronectin-fibronectin binding site, and is capable of inhibiting fibronectin matrix assembly. In contrast to previously identified fibronectin fragments that block fibronectin matrix assembly by blocking an initial event in matrix assembly (i.e., fibronectin binding to cells), the invention polypeptide appears to inhibit an intermediate step in matrix assembly, i.e., fibronectin self-association prior to the disulfide cross-linking that stabilizes the fibronectin matrix.
摘要翻译：根据本发明，提供了衍生自纤连蛋白的第一III型重复序列的新型合成多肽。本发明的合成多肽包括纤连蛋白 - 纤连蛋白结合位点，并能够抑制纤连蛋白基质组装。与通过阻断基质组装中的初始事件（即，纤连蛋白与细胞结合）来阻断纤连蛋白基质组装的先前鉴定的纤连蛋白片段相反，本发明多肽似乎抑制基质组装中的中间步骤，即纤连蛋白自身结合之前二硫键交联使纤连蛋白基质稳定。

23. 发明授权

US5180808A Versican core protein, nucleic acid sequences encoding the same, nucleic acid probes, anti-versican antibodies, and methods of detecting the same 失效
标题翻译：维生素C蛋白，编码其的核酸序列，核酸探针，抗体抗体及其检测方法
公开(公告)号：US5180808A
公开(公告)日：1993-01-19
申请号：US441179
申请日：1989-11-27
申请人： Erkki I. Ruoslahti
发明人： Erkki I. Ruoslahti
IPC分类号： C07K14/78 , C07K16/18 , C12N15/12 , G01N33/53
CPC分类号： C07K14/78 , C07K16/18 , G01N33/5308
摘要： The invention relates to the fibroblast proteoglycan, versican. The versican core protein has the amino acid sequence, and is encoded by the nucleotide sequence, as shown in FIG. 1. The nucleotide sequence and method of hyaluronic acid binding domain is provided and methods of preparing recombinant proteins having hyaluronic acid binding activity are provided. Such protein can be used to determine the presence of hyaluronic acid and as a vehicle to bring other molecules in contact with hyaluronic acid. The complete versican sequence will allow the production of the entire versican molecule to be used, for example, in tissue reconstruction. Nucleic acid probes are provided which are useful for detecting nucleic acid sequences encoding versican. The invention also provides antibodies reactive with versican.
摘要翻译：本发明涉及成纤维细胞蛋白多糖。多核心蛋白具有氨基酸序列，并由核苷酸序列编码，如图1所示。提供透明质酸结合结构域的核苷酸序列和方法，并提供制备具有透明质酸结合活性的重组蛋白的方法。这样的蛋白质可以用于确定透明质酸的存在和作为使其他分子与透明质酸接触的载体。完整的多肽序列将允许使用整个多肽分子的生产，例如在组织重建中。提供了核酸探针，其可用于检测编码versican的核酸序列。本发明还提供与多巴胺反应的抗体。

24. 发明授权

US07067619B2 Integrin-binding peptides 失效
标题翻译：整合素结合肽
公开(公告)号：US07067619B2
公开(公告)日：2006-06-27
申请号：US09364597
申请日：1999-07-30
申请人： Erkki I Ruoslahti , Erkki Koivunen
发明人： Erkki I Ruoslahti , Erkki Koivunen
IPC分类号： C07K7/00 , C07K7/64
CPC分类号： C07K14/70546 , A61K38/00 , A61L27/227 , C07K14/75 , C07K14/78
摘要： This invention is directed to novel integrin binding peptides. These peptides bind to αv- of α5-containing integrins and can exhibit high binding affinity. They contain one of the following sequence motifs: RX1ETX2WX3 [SEQ ID NO:1] (especially RRETAWA [SEQ ID NO:8]); RGDGX [SEQ ID NO:2], in which X is an amino acid with a hydrophobic, aromatic side chain; the double cyclic CX1CRGDCX2C [SEQ ID NO:15]; and RLD. The peptides generally exhibit their highest binding affinity when they assume a conformationally stabilized configuration. This invention also provides methods of using these peptides.
摘要翻译：本发明涉及新型整联蛋白结合肽。这些肽结合含α5 N的整联蛋白的α-α，并且可表现出高的结合亲和力。它们含有以下序列基序之一：RX ETX WX 3

25. 发明授权

US06436900B1 Methods of treating a pathology or a fibrotic condition by administering decorin 失效
标题翻译：通过管理核心蛋白聚糖治疗病理或纤维化病症的方法
公开(公告)号：US06436900B1
公开(公告)日：2002-08-20
申请号：US08458864
申请日：1995-06-02
申请人： Erkki I. Ruoslahti , Yu Yamaguchi
发明人： Erkki I. Ruoslahti , Yu Yamaguchi
IPC分类号： A61K3816
CPC分类号： C07K14/4703 , A61K38/00 , C07K14/4725 , C07K2319/00
摘要： The present invention provides methods of treating pathologies using Decorin.
摘要翻译：本发明提供使用Decorin治疗病理学的方法

26. 发明授权

US06277812B1 Methods for inhibiting TGF-&bgr; activity 失效
标题翻译：抑制TGF-β活性的方法
公开(公告)号：US06277812B1
公开(公告)日：2001-08-21
申请号：US08458834
申请日：1995-06-02
申请人： Erkki I. Ruoslahti , Yu Yamaguchi
发明人： Erkki I. Ruoslahti , Yu Yamaguchi
IPC分类号： A01N6100
CPC分类号： C07K14/4703 , A61K38/00 , C07K14/4725 , C07K14/475 , C07K14/495 , C07K2319/00
摘要： The present invention provides a method of inhibiting an activity of a cell regulatory factor comprising contacting the cell regulatory factor with a purified polypeptide, wherein the polypeptide comprises the cell regulatory factor binding domain of a protein and wherein the protein is characterized by a leucine-rich repeat of about 24 amino acids. In a specific embodiment, the present invention relates to the ability of decorin, a 40,000 dalton protein that usually carries a glycosaminoglycan chain, to bind TGF-&bgr;. The invention also provides a novel cell regulatory factor designated MRF. Also provided are methods of identifying, detecting and purifying cell regulatory factors and proteins which bind and affect the activity of cell regulatory factors. The present invention further relates to methods for the prevention or reduction of scarring by administering decorin or a functional equivalent of decorin to a wound. The methods are particularly useful for dermal wounds resulting from burns, injuries or surgery. In addition, the present invention includes pharmaceutical compositions containing decorin or its functional equivalent and a pharmaceutically acceptable carrier useful in such methods. Finally, methods for preventing or inhibiting pathological conditions by administering decorin are also provided.
摘要翻译：本发明提供了抑制细胞调节因子活性的方法，包括使细胞调节因子与纯化的多肽接触，其中所述多肽包含蛋白质的细胞调节因子结合结构域，其中所述蛋白质的特征在于富含亮氨酸重复约24个氨基酸。在一个具体实施方案中，本发明涉及核心蛋白聚糖，通常携带糖胺聚糖链的40,000道尔顿蛋白结合TGF-β的能力。本发明还提供了称为MRF的新型细胞调节因子。还提供鉴定，检测和纯化结合并影响细胞调节因子活性的细胞调节因子和蛋白质的方法。本发明还涉及通过给予伤口的核心蛋白聚糖或核心蛋白聚糖的功能等同物来预防或减少瘢痕形成的方法。这些方法对于由烧伤，损伤或手术引起的皮肤伤口特别有用。此外，本发明包括含有核心蛋白聚糖蛋白聚糖或其功能等同物的药物组合物和可用于这些方法的药学上可接受的载体。最后，还提供了通过管理核心蛋白聚糖来预防或抑制病理状况的方法。

27. 发明授权

US5952224A Selection of cells having increased cell adhesion properties 失效
标题翻译：选择具有增加的细胞粘附性质的细胞
公开(公告)号：US5952224A
公开(公告)日：1999-09-14
申请号：US915315
申请日：1997-08-20
申请人： Michael D. Pierschbacher , Erkki I. Ruoslahti , Shoukat Dedhar
发明人： Michael D. Pierschbacher , Erkki I. Ruoslahti , Shoukat Dedhar
IPC分类号： G01N33/53 , C12N5/00 , C12N5/02 , C12N5/09 , C12Q1/04
CPC分类号： C12N5/0693 , C12N5/0018 , C12N2501/58 , C12N2501/998
摘要： A method for selecting cell lines expressing increased cell adhesion properties or promoting cell differentiation by culturing cells in increasing concentrations of adhesion ligand containing media. Cell lines produced by such method.
摘要翻译：通过增加浓度的粘附配体的培养基来选择表达增加的细胞粘附性或促进细胞分化的细胞系的方法。通过这种方法生产的细胞系。

28. 发明授权

US5700908A .beta..sub.3 integrin cytoplasmic domain specific peptide and nucleic acid 失效
标题翻译： β3整合素胞质区特异性肽和核酸
公开(公告)号：US5700908A
公开(公告)日：1997-12-23
申请号：US459246
申请日：1995-06-01
申请人： Erkki I. Ruoslahti
发明人： Erkki I. Ruoslahti
IPC分类号： C07K14/705 , C07H21/04
CPC分类号： C07K14/70557 , Y10S530/806
摘要： The invention is directed to a peptide having the amino acid sequence of the cytoplasmic domain of integrin subunit .beta..sub.3 ', KFEEERARAKWDTVRDGAGRFLKSLV, or subsequences thereof, and the nucleic acid encoding that peptide.
摘要翻译：本发明涉及具有整联蛋白亚基β3'，KFEEERARAKWDTVRDGAGRFLKSLV或其子序列的细胞质结构域的氨基酸序列的肽和编码该肽的核酸。

29. 发明授权

US5629291A Methods of modulating fibronectin extracellular matrix assembly 失效
标题翻译：调节纤连蛋白细胞外基质组装的方法
公开(公告)号：US5629291A
公开(公告)日：1997-05-13
申请号：US340812
申请日：1994-11-17
申请人： Erkki I. Ruoslahti , Alex Morla
发明人： Erkki I. Ruoslahti , Alex Morla
IPC分类号： A61K38/00 , C07K14/78 , A61K38/17 , C12N5/02
CPC分类号： C07K14/78 , A61K38/00
摘要： The present invention provides fibronectin self-assembly sites. The invention provides a set of polypeptides derived from the first type III repeat of fibronectin which contain a fibronectin-fibronectin binding site. These polypeptides have been used to obtain a second set of polypeptides derived from the C-terminal type I repeats which contain a second fibronectin-fibronectin binding site which interacts with the first type III repeat of fibronectin. These polypeptides are capable of inhibiting fibronectin matrix assembly by interfering with fibronectin-fibronectin binding. These polypeptides are also capable of enhancing fibronectin matrix assembly and inducing disulfide cross-linking of fibronectin molecules in vitro. In addition, these polypeptides are capable of inhibiting migration of tumor cells. The polypeptides of the present invention have a number of related uses as well.
摘要翻译：本发明提供纤连蛋白自组装位点。本发明提供一组衍生自含有纤连蛋白 - 纤连蛋白结合位点的纤连蛋白的第一III型重复序列的多肽。已经使用这些多肽获得源自C末端I型重复序列的第二组多肽，其含有与纤连蛋白的第一III型重复相互作用的第二纤连蛋白 - 纤连蛋白结合位点。这些多肽能够通过干扰纤连蛋白 - 纤连蛋白结合来抑制纤连蛋白基质组装。这些多肽还能够体外增强纤连蛋白基质的组装和诱导纤连蛋白分子的二硫键交联。此外，这些多肽能够抑制肿瘤细胞的迁移。本发明的多肽也具有许多相关用途。

30. 发明授权

US5591592A Method for detection of fibronectin receptor ligands 失效
标题翻译：纤连蛋白受体配体检测方法
公开(公告)号：US5591592A
公开(公告)日：1997-01-07
申请号：US279279
申请日：1994-07-22
申请人： Erkki I. Ruoslahti , Guido Tarone , Filippo G. Giancotti , Bruce E. Vogel
发明人： Erkki I. Ruoslahti , Guido Tarone , Filippo G. Giancotti , Bruce E. Vogel
IPC分类号： C07K14/435 , A61K38/00 , C07K1/16 , C07K1/22 , C07K14/705 , C07K16/00 , C07K16/28 , C12N15/02 , C12P21/00 , C12P21/08 , C12R1/91 , G01N33/53 , G01N33/566 , G01N33/539 , G01N33/543
CPC分类号： C07K14/7055 , A61K38/00
摘要： The present invention provides a substantially pure integrin characterized in that it consists of an .alpha..sub.v and a .beta..sub.1 subunit. The receptor binds to fibronectin and GRGDSPK but does not bind to vitronectin. The .alpha..sub.v .beta..sub.1 receptor can be used to determine the presence of ligands for the receptor.
摘要翻译：本发明提供了基本上纯的整联蛋白，其特征在于其由αv和β1亚基组成。受体结合纤连蛋白和GRGDSPK，但不结合玻连蛋白。 αvβ1受体可用于确定受体配体的存在。

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