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21. 发明授权

US06617113B2 Methods of determining the presence of double stranded nucleic acids in a sample 有权
公开(公告)号：US06617113B2
公开(公告)日：2003-09-09
申请号：US10151630
申请日：2002-05-16
申请人： David W. Deamer
发明人： David W. Deamer
IPC分类号： C12Q168
CPC分类号： G01N33/48721 , C12N15/1003 , C12Q1/6813 , C12Q1/6825 , C12Q1/6837 , C12Q2545/114 , C12Q2565/631 , C12Q2565/525
摘要： Methods for determining the presence of double stranded nucleic acids in a sample are provided. In the subject methods, nucleic acids present in a fluid sample are translocated through a nanopore, e.g. by application of an electric field to the fluid sample. The current amplitude through the nanopore is monitored during the translocation process and changes in the amplitude are related to the passage of single- or double-stranded molecules through the nanopore. The subject methods find use in a variety of applications in which the detection of the presence of double-stranded nucleic acids in a sample is desired, e.g. in hybridization assays, such as Northern blot assays, Southern blot assays, array based hybridization assays, etc.

22. 发明授权

US06428959B1 Methods of determining the presence of double stranded nucleic acids in a sample 有权
标题翻译：确定样品中双链核酸存在的方法
公开(公告)号：US06428959B1
公开(公告)日：2002-08-06
申请号：US09656302
申请日：2000-09-06
申请人： David W. Deamer
发明人： David W. Deamer
IPC分类号： C12Q168
CPC分类号： G01N33/48721 , C12N15/1003 , C12Q1/6813 , C12Q1/6825 , C12Q1/6837 , C12Q2545/114 , C12Q2565/631 , C12Q2565/525
摘要： Methods for determining the presence of double stranded nucleic acids in a sample are provided. In the subject methods, nucleic acids present in a fluid sample are translocated through a nanopore, e.g. by application of an electric field to the fluid sample. The current amplitude through the nanopore is monitored during the translocation process and changes in the amplitude are related to the passage of single- or double-stranded molecules through the nanopore. The subject methods find use in a variety of applications in which the detection of the presence of double-stranded nucleic acids in a sample is desired, e.g. in hybridization assays, such as Northern blot assays, Southern blot assays, array based hybridization assays, etc.
摘要翻译：提供了确定样品中双链核酸存在的方法。在本发明方法中，存在于流体样品中的核酸通过纳米孔被转位，例如，通过向流体样品施加电场。在易位过程中监测通过纳米孔的电流幅度，并且振幅的变化与单链或双链分子通过纳米孔的通过有关。本发明的方法可用于各种应用，其中需要检测样品中双链核酸的存在，例如，在杂交测定中，例如Northern印迹测定，Southern印迹测定，基于阵列的杂交测定等

23. 发明授权

US10059988B2 Methods for using a nanopore 有权
公开(公告)号：US10059988B2
公开(公告)日：2018-08-28
申请号：US13615183
申请日：2012-09-13
申请人： Mark A. Akeson , David W. Deamer , Seico Benner , William B. Dunbar , Noah A. Wilson , Kathy Lieberman , Robin Abu-Shumays , Nicholas Hurt
发明人： Mark A. Akeson , David W. Deamer , Seico Benner , William B. Dunbar , Noah A. Wilson , Kathy Lieberman , Robin Abu-Shumays , Nicholas Hurt
IPC分类号： C12Q1/68 , C12Q1/6874 , C12Q1/6869 , G01N33/487 , C25B3/10 , C12Q1/54 , G01N27/327 , G01N27/416
CPC分类号： C12Q1/6874 , C12Q1/54 , C12Q1/6869 , C25B3/10 , G01N27/3278 , G01N27/4166 , G01N33/48721 , C12Q2522/101 , C12Q2565/631
摘要： Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

24. 发明申请

US20120160687A1 CHARACTERIZATION OF INDIVIDUAL POLYMER MOLECULES BASED ON MONOMER-INTERFACE INTERACTIONS 审中-公开
标题翻译：基于单体界面相互作用的个体聚合物分子的表征
公开(公告)号：US20120160687A1
公开(公告)日：2012-06-28
申请号：US13186966
申请日：2011-07-20
申请人： Mark Akeson , Daniel Branton , George Church , David W. Deamer
发明人： Mark Akeson , Daniel Branton , George Church , David W. Deamer
IPC分类号： C07K1/26
CPC分类号： C12Q1/6869 , G01N27/447 , G01N33/48721 , C12Q2565/631 , C12Q2565/607
摘要： The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and a interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel.
摘要翻译：本发明涉及一种用于通过（1）在两个池之间提供两个独立的相邻的介质池和界面来检测核酸中的双链区域的方法，所述界面具有如此尺寸以允许顺序单体单链核酸但不是双链核酸的单体通过从一个池到另一个池; （2）将核酸聚合物置于两个池中的一个中; 和（3）测量，因为单链核酸聚合物的每个核苷酸单体通过通道，以便在进入通道之前与另一个核苷酸单体杂交的核苷酸单体与未杂交的核苷酸单体区分进入通道之前的另一种核苷酸单体。

25. 发明申请

US20100267026A1 METHODS AND APPARATUS FOR CHARACTERIZING POLYNUCLEOTIDES 有权
标题翻译：用于表征多核苷酸的方法和装置
公开(公告)号：US20100267026A1
公开(公告)日：2010-10-21
申请号：US12626014
申请日：2009-11-25
申请人： Mark Akeson , Daniel Branton , David W. Deamer , Jeffrey R. Sampson
发明人： Mark Akeson , Daniel Branton , David W. Deamer , Jeffrey R. Sampson
IPC分类号： C12Q1/68
CPC分类号： G01N33/48721 , B01L3/502707 , B01L3/502761 , B01L2300/0896 , B01L2400/0475 , B82Y5/00 , B82Y30/00 , C12Q1/6869 , C12Q2565/631 , C12Q2521/543
摘要： Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.
摘要翻译：提供了用于分析聚合物的系统和方法，例如多核苷酸。该系统能够以指定的速率分析聚合物。一个这样的分析系统包括具有纳米孔孔的结构和邻近纳米孔孔的分子马达，例如聚合酶。

26. 发明授权

US07238485B2 Methods and apparatus for characterizing polynucleotides 有权
标题翻译：表征多核苷酸的方法和装置
公开(公告)号：US07238485B2
公开(公告)日：2007-07-03
申请号：US11088140
申请日：2005-03-23
申请人： Mark Akeson , Daniel Branton , David W. Deamer , Jeffrey R. Sampson
发明人： Mark Akeson , Daniel Branton , David W. Deamer , Jeffrey R. Sampson
IPC分类号： C12Q1/68 , C12M1/00 , C07H21/02 , C07H21/04
CPC分类号： G01N33/48721 , B01L3/502707 , B01L3/502761 , B01L2300/0896 , B01L2400/0475 , B82Y5/00 , B82Y30/00 , C12Q1/6869 , C12Q2565/631 , C12Q2521/543
摘要： Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.
摘要翻译：提供了用于分析聚合物的系统和方法，例如多核苷酸。该系统能够以指定的速率分析聚合物。一个这样的分析系统包括具有纳米孔孔的结构和邻近纳米孔孔的分子马达，例如聚合酶。

27. 发明授权

US5795782A Characterization of individual polymer molecules based on monomer-interface interactions 失效
标题翻译：基于单体界面相互作用的单个聚合物分子的表征
公开(公告)号：US5795782A
公开(公告)日：1998-08-18
申请号：US405735
申请日：1995-03-17
申请人： George Church , David W. Deamer , Daniel Branton , Richard Baldarelli , John Kasianowicz
发明人： George Church , David W. Deamer , Daniel Branton , Richard Baldarelli , John Kasianowicz
IPC分类号： C07K14/705 , C12N9/12 , C12Q1/68 , G01N27/04 , G01N33/68 , G01N33/483
CPC分类号： G01N33/68 , C07K14/705 , C12N9/1241 , C12Q1/6869 , G01N33/48721 , B82Y30/00 , Y10S977/781 , Y10S977/788 , Y10S977/84 , Y10S977/88 , Y10S977/924 , Y10S977/953
摘要： A method is disclosed for characterizing a linear polymer molecule by measuring physical changes across an interface between two pools of media as the linear polymer traverses the interface and monomers of the polymer interact with the interface, where the physical changes are suitable to identify characteristics of the polymer.
摘要翻译：公开了一种用于表征线性聚合物分子的方法，当线性聚合物穿过界面并测量聚合物的单体与界面相互作用时，通过测量两个介质池之间的界面上的物理变化，其中物理变化适合于识别聚合物。

28. 发明授权

US5792668A Radio frequency spectral analysis for in-vitro or in-vivo environments 失效
标题翻译：射频频谱分析体外或体内环境
公开(公告)号：US5792668A
公开(公告)日：1998-08-11
申请号：US631916
申请日：1996-04-15
申请人： Milton E. Fuller , David W. Deamer , Mark N. Iverson , Ajit J. Koshy, deceased
发明人： Milton E. Fuller , David W. Deamer , Mark N. Iverson , Ajit J. Koshy, deceased
IPC分类号： A61B5/00 , A61B5/05 , A61B5/053 , G01N22/00 , G01N33/487 , G01N27/00 , G01N33/50
CPC分类号： A61B5/14532 , A61B5/053 , Y10T436/144444 , Y10T436/171538
摘要： Concentration of a target chemical in the presence of other substances in a specimen is determined by subjecting the specimen to radio frequency electromagnetic components, sequentially or otherwise, ranging to about 5 GHz. The reflected and/or transmitted signal real and imaginary components at the specimen are spectrally examined as a function of frequency to identify the presence and/or concentration of the chemical of interest. Such examination includes analysis of the effective complex impedance presented by the specimen, and/or effective phase shift between the transmitted and reflected signal at the specimen. The effects upon glucose concentration measurements of varying electrolytes, primarily NaCl, can be nulled-out by examining impedance magnitude at a cross-over frequency, for example about 2.5 GHz. NaCl concentration exhibits a very linear relationship with phase shift change at frequencies in the 2 GHz-3 GHz range. In a specimen that is blood, such phase shift measurements provide data proportional to NaCl concentration. Impedance magnitude measurements using 1 MHz to 400 MHz frequencies provides a measure of combined concentration of glucose and NaCl. The phase shift data may then be used to substrate out the NaCl concentration from the combined concentration, to yield a good measure of glucose concentration. Such tests may be conducted in-vitro or in-vivo and lend themselves to blood level glucose analyses by diabetics.
摘要翻译：在样本中存在其他物质的情况下，目标化学物质的浓度是通过对样本进行射频电磁成分的测定，依次或其他方式，范围为约5 GHz。在样品处的反射和/或传输的信号实部和虚部被频谱地检查作为频率的函数，以识别感兴趣的化学物质的存在和/或浓度。这种检查包括对样品所呈现的有效复阻抗的分析和/或样品的透射和反射信号之间的有效相移。通过检查交叉频率（例如约2.5 GHz）处的阻抗幅度，可以对不同电解质（主要是NaCl）对葡萄糖浓度测量的影响无效。 NaCl浓度与2 GHz -3 GHz频段的相移变化呈非常线性关系。在血液样本中，这种相移测量提供与NaCl浓度成比例的数据。使用1 MHz至400MHz频率的阻抗幅度测量提供了葡萄糖和NaCl的组合浓度的量度。然后可以将相移数据用于从组合浓度中提取NaCl浓度，以产生良好的葡萄糖浓度测量值。这样的测试可以在体外或体内进行，并且可以借助糖尿病患者进行血液葡萄糖分析。

29. 发明授权

US4829989A Stoop laborer's body support having hinge with adjustable spring biasing 失效
标题翻译：具有可调节弹簧偏置的铰链的Stoop laborer的身体支撑
公开(公告)号：US4829989A
公开(公告)日：1989-05-16
申请号：US35370
申请日：1987-04-07
申请人： Richard M. Deamer , David W. Deamer
发明人： Richard M. Deamer , David W. Deamer
IPC分类号： A61F5/02 , A63B21/045 , A63B23/02
CPC分类号： A63B21/00181 , A61F5/02 , A63B21/0455 , A63B21/4025 , A63B2208/02 , A63B23/0211 , A63B23/0233
摘要： A portable spring leveraged device adapted for attachment to the hips in a manner that allows a person engaged in so-called "stoop labor" to bend or flex at the hips with less physical exertion, and with less stress and strain on involved musculature and vertebral joints. The device consists of a U-shaped metal or plastic frame, hinged in each arm of the U and provided with spring resistance at each hinge point. The frame is belt-mounted at the wearer's waist with the hinge points adjacent the hips and with the bottom of the U and arms providing padded contact at the wearer's chest and thighs, respectively. The two arms provide for independent leg movement for walking while the chest contact resiliently supports a major portion of the wearer's upper torso weight during leaning and stooping, thus relieving the stresses and strains normally imparted to the back muscles, ligamentous and bony support, and especially the low back structure, during, for example, use of a short hoe and/or lifting produce boxes.
摘要翻译：一种便携式弹簧杠杆装置，其适于以允许所谓的“劳动劳动”的人在臀部弯曲或弯曲的方式附接到臀部，同时体力消耗较少，并且对所涉及的肌肉和椎体的应力和应变较小关节。该装置由U型金属或塑料框架组成，铰链在U的每个臂上，并在每个铰链点处具有弹簧阻力。框架安装在穿戴者的腰部，铰链位于臀部附近，U形底部分别在佩戴者的胸部和大腿处提供垫层接触。两个手臂提供独立的腿部运动，而胸部接触在倾斜和弯曲期间弹性地支撑穿戴者的上身重量的主要部分，从而减轻通常赋予背部肌肉，韧带和骨骼支撑的应力和应变，特别是在例如使用短锄头和/或提升产品箱的过程中的低背部结构。

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