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21. 发明申请

US20140096283A1 Plants Having Enhanced Yield-Related Traits and a Method for Making the Same 审中-公开
标题翻译：具有增强产量相关性状的植物及其制作方法
公开(公告)号：US20140096283A1
公开(公告)日：2014-04-03
申请号：US14035211
申请日：2013-09-24
申请人： BASF Plant Science GmbH
发明人： Christophe Reuzeau , Valerie Frankard , Yves Hatzfeld , Ana Isabel Sanz Molinero
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , Y02A40/146
摘要： The present invention relates generally to the field of molecular biology and concerns a method for enhancing various yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a TCP1 or a TCP2 transcription factor, an Epsin-like polypeptide, a tRNA delta(2)-isopentenylpyrophosphate transferase (IPPT) polypeptide, or a SHORT-ROOT (SHR) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding a TCP1 or TCP2 polypeptide, an Epsin-like polypeptide, a tRNA delta(2)-isopentenylpyrophosphate transferase (IPPT) polypeptide, or a SHORT-ROOT (SHR) polypeptide, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in performing the methods of the invention.
摘要翻译：本发明一般涉及分子生物学领域，涉及通过调节植物中编码TCP1或TCP2转录因子，类似蛋白样样多肽的核酸的表达来增强植物中各种产量相关性状的方法， tRNA（2） - 异戊烯基焦磷酸转移酶（IPPT）多肽或SHORT-ROOT（SHR）（SHR）多肽。本发明还涉及已经调节了编码TCP1或TCP2多肽，类似蛋白样样多肽，tRNAδ（2） - 异戊烯基焦磷酸转移酶（IPPT）多肽或SHORT-ROOT（SHR）多肽的核酸表达的植物，哪些植物相对于相应的野生型植物或其他对照植物具有增强的产量相关性状。本发明还提供了用于实施本发明方法的构建体。

22. 发明申请

US20130104261A1 Arabidopsis Genes Encoding Proteins Involved in Sugar and Lipid Metabolism and Methods of Use 审中-公开
标题翻译：编码涉及糖和脂质代谢的蛋白质的拟南芥基因和使用方法
公开(公告)号：US20130104261A1
公开(公告)日：2013-04-25
申请号：US13719301
申请日：2012-12-19
申请人： BASF Plant Science GmbH , Michigan State University
发明人： Carl Andre , Alex Cernac , Christoph Benning , Heiko A. Haertel , Volker Mittendorf
IPC分类号： A01H1/06
CPC分类号： A01H1/06 , C07K14/415 , C12N15/8245 , C12N15/8247 , C12N15/8251 , C12N15/8261 , Y02A40/146
摘要： Isolated nucleic acids and polypeptides associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana are provided. The nucleic acids and polypeptides are used in methods of producing transgenic plants and modulating levels of seed storage compounds in a plant. Preferably, the seed storage compounds are lipids, fatty acids, starches, or seed storage proteins.
摘要翻译：提供了与脂质和糖代谢调节相关的分离的核酸和多肽。特别地，提供了来自拟南芥的脂质代谢蛋白（LMP）和编码核酸。核酸和多肽用于产生转基因植物和调节植物中种子储存化合物水平的方法中。优选地，种子储存化合物是脂质，脂肪酸，淀粉或种子储存蛋白。

23. 发明申请

US20220162629A1 Process for the Production of Polyunsaturated Fatty Acids in Transgenic Organisms 有权
公开(公告)号：US20220162629A1
公开(公告)日：2022-05-26
申请号：US17532634
申请日：2021-11-22
申请人： BASF PLANT SCIENCE GMBH
发明人： Thorsten Zank , Jörg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Schmidt , Helene Kirsch
IPC分类号： C12N15/82 , C12N9/02 , C12N9/10 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , A23D9/00 , A23L33/115 , A23D9/02 , C11B1/00
摘要： The present invention relates to a process for the production of polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids which encode polypeptides with Δ5-elongase activity. Advantageously, these nucleic acids can be expressed in the organism together with further nucleic acids which encode polypeptides of the biosynthesis of the fatty acid or lipid metabolism. Especially advantageous are nucleic acids which encode Δ6-desaturases, Δ5-desaturases, Δ4-desaturases and/or Δ6-elongases. These desaturases and elongases are advantageously derived from Thalassiosira, Euglena or Ostreococcus. The invention furthermore relates to a process for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids, and oils and/or triacylglycerides thus obtained. The invention also relates to the nucleic acids, and constructs, vectors and transgenic organisms comprising the same, as well as oils, lipids and/or fatty acids produced by the process according to the invention and to their use.

24. 发明授权

US11180769B2 Method for the production of multiple-unsaturated fatty acids in transgenic organisms 有权
公开(公告)号：US11180769B2
公开(公告)日：2021-11-23
申请号：US16509247
申请日：2019-07-11
申请人： BASF Plant Science GmbH
发明人： Thorsten Zank , Jörg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Schmidt , Helene Kirsch
IPC分类号： C12N15/82 , C12N9/02 , C12N9/10 , C12P7/64 , A23D9/00 , A23L33/115 , A23D9/02 , C11B1/00
摘要： The present invention relates to a process for the production of polyunsaturated fatty acids in an organism by introducing, into the organism, nucleic acids which encode polypeptides with Δ5-elongase activity. Advantageously, these nucleic acids can be expressed in the organism together with further nucleic acids which encode polypeptides of the biosynthesis of the fatty acid or lipid metabolism. Especially advantageous are nucleic acids which encode Δ6-desaturases, Δ5-desaturases, Δ4-desaturases and/or Δ6-elongases. These desaturases and elongases are advantageously derived from Thalassiosira, Euglena or Ostreococcus. The invention furthermore relates to a process for the production of oils and/or triacylglycerides with an elevated content of long-chain polyunsaturated fatty acids, and oils and/or triacylglycerides thus obtained. The invention also relates to the nucleic acids, and constructs, vectors and transgenic organisms comprising the same, as well as oils, lipids and/or fatty acids produced by the process according to the invention and to their use.

25. 发明申请

US20150368663A1 METHOD FOR ISOLATION OF TRANSCRIPTION TERMINATION SEQUENCES 审中-公开
标题翻译：用于分离转录终止序列的方法
公开(公告)号：US20150368663A1
公开(公告)日：2015-12-24
申请号：US14821872
申请日：2015-08-10
申请人： BASF Plant Science GmbH
发明人： Hee-Sook Song , Michael Kock , Jeffrey A. Brown , Linda Patricia Loyall , Liqun Xing , Hongmei Jia , John McMillan , Lesley Ireland
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C12N15/1051 , C12N15/82 , C12N15/8216
摘要： The invention relates to efficient, high-throughput methods, systems, and DNA constructs for identification and isolation of transcription termination sequences. The invention relates further to specific terminator sequences identified by said methods isolated from rice.
摘要翻译：本发明涉及用于鉴定和分离转录终止序列的有效的高通量方法，系统和DNA构建体。本发明进一步涉及通过从水稻中分离的所述方法鉴定的特异性终止子序列。

26. 发明申请

US20150361404A1 METHOD FOR PRODUCING POLYUNSATURATED FATTY ACIDS IN TRANSGENIC PLANTS 审中-公开
标题翻译：在转基因植物中生产聚氨酯脂肪酸的方法
公开(公告)号：US20150361404A1
公开(公告)日：2015-12-17
申请号：US14823253
申请日：2015-08-11
申请人： BASF Plant Science GmbH
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Nagamani Datla
IPC分类号： C12N9/02 , A23D9/00 , A61K31/202 , C12N15/82 , A61K8/36
CPC分类号： C12N9/0071 , A23D9/00 , A61K8/361 , A61K8/922 , A61K31/202 , A61K2800/86 , A61Q19/00 , C12N9/0083 , C12N9/1029 , C12N15/8247 , C12Y114/19
摘要： The present invention relates to a process for the production of polyunsaturated fatty acids in the seed of transgenic plants by introducing, into the organism, nucleic acids which encode polypeptides with a ω3-desaturase, Δ12-desaturase, Δ6-desaturase, Δ6-elongase, Δ5-desaturase, Δ5-elongase and/or Δ4-desaturase activity. The invention furthermore relates to recombinant nucleic acid molecules comprising the nucleic acid sequences which encode the aforementioned polypeptides, either jointly or individually, and transgenic plants which comprise the aforementioned recombinant nucleic acid molecules. Furthermore, the invention relates to the generation of a transgenic plant and to oils, lipids and/or fatty acids with an elevated content of polyunsaturated fatty acids, in particular arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid, as the result of the expression of the elongases and desaturases used in the process according to the invention.
摘要翻译：本发明涉及一种在转基因植物种子中生产多不饱和脂肪酸的方法，其通过向生物体中引入用ω3-去饱和酶，D-去饱和酶，6-去饱和酶，＆Dgr; 6-延伸酶，＆Dgr; 5-去饱和酶，＆Dgr; 5-延伸酶和/或＆Dgr; 4-去饱和酶活性。本发明还涉及包含共同或单独编码前述多肽的核酸序列的重组核酸分子以及包含上述重组核酸分子的转基因植物。此外，本发明涉及转基因植物的产生以及具有升高的多不饱和脂肪酸含量的油，脂和/或脂肪酸，特别是花生四烯酸，二十碳五烯酸和/或二十二碳六烯酸，作为表达的结果的根据本发明的方法中使用的延伸酶和去饱和酶。

27. 发明申请

US20150223419A1 METHOD OF IMPROVED PLANT BREEDING 审中-公开
标题翻译：改良植物育种方法
公开(公告)号：US20150223419A1
公开(公告)日：2015-08-13
申请号：US14624796
申请日：2015-02-18
申请人： BASF Plant Science GmbH
发明人： Pierre Lejeune , Frederik Leyns , Cedrick Vandaele , Wim Van Caeneghem
IPC分类号： A01H1/04 , G06T7/00 , B65G43/00 , A01G9/02 , G05B15/02
CPC分类号： A01H1/04 , A01G9/02 , B65G15/005 , B65G15/14 , B65G43/00 , G05B15/02 , G06T7/0012 , G06T2207/30188
摘要： An improved plant breeding system for high throughput analysis of plant phenotype and genotype is provided. A method for analyzing the impact of genetic modifications on plants and selecting a plant with a genetic modification of interest is also provided. Also provided is a method for developing marketable information for improved plant breeding and a method for collecting data on a selected plant phenotype for rapid analysis of the effect of a genetic modification on the selected phenotype.
摘要翻译：提供了一种改进的植物育种系统，用于植物表型和基因型的高通量分析。还提供了分析遗传修饰对植物和选择感兴趣的遗传修饰的植物的影响的方法。还提供了用于开发用于改进植物育种的可销售信息的方法以及用于收集关于所选植物表型的数据的方法，用于快速分析遗传修饰对所选表型的影响。

28. 发明申请

US20150211032A1 ISOLATION AND CHARACTERIZATION OF A NOVEL PYTHIUM OMEGA 3 DESATURASE WITH SPECIFICITY TO ALL OMEGA 6 FATTY ACIDS LONGER THAN 18 CARBON CHAINS 审中-公开
标题翻译：一个新的磷酸三钙脱硫剂的分离和表征对所有欧米茄6脂肪酸长18个碳链具有特异性
公开(公告)号：US20150211032A1
公开(公告)日：2015-07-30
申请号：US14684682
申请日：2015-04-13
申请人： BASF Plant Science GmbH , Bioriginal Food & Science Corp.
发明人： Jörg Bauer , Guohai Wu , Xiao Qiu
IPC分类号： C12P7/64 , A61K31/202 , A23K1/16 , A61K8/92
CPC分类号： C12P7/6472 , A61K8/64 , A61K8/922 , A61K31/202 , A61K2800/10 , A61Q19/00 , C12N9/0006 , C12N9/0083 , C12N9/0093 , C12N15/52 , C12P7/6427 , C12P7/6436
摘要： The present invention relates to a polynucleotide encoding an omega 3 (ω-3) desaturase from Pythium irregulare with specificity to long chain polyunsaturated omega 6 (ω-6) fatty acids as well as a vector containing the polynucleotide, and a host cell containing the vector or the polynucleotide. Moreover, the present invention pertains to a polypeptide encoded by the polynucleotide, antibodies against the polypeptide as well as a method for the manufacture of the polypeptide. Further, encompassed by the present invention are transgenic non-human organisms. Finally, the present invention relates to methods for the manufacture of compounds and oil- fatty acid-, or lipid-containing compositions.
摘要翻译：本发明涉及编码具有对长链多不饱和ω-6（ω-6）脂肪酸的特异性的非腐霉菌的ω3（ω-3）去饱和酶的多核苷酸以及含有该多核苷酸的载体，以及含有载体或多核苷酸。此外，本发明涉及由多核苷酸编码的多肽，针对多肽的抗体以及制备多肽的方法。此外，本发明包括转基因非人生物体。最后，本发明涉及制备化合物和油 - 脂肪酸或含脂质组合物的方法。

29. 发明申请

US20150143584A1 NUCLEIC ACID MOLECULES ENCODING POLYPEPTIDES INVOLVED IN REGULATION OF SUGAR AND LIPID METABOLISM AND METHODS OF USE VIII 审中-公开
标题翻译：编码参与糖和脂肪代谢调节的多肽的核酸分子及其使用方法VIII
公开(公告)号：US20150143584A1
公开(公告)日：2015-05-21
申请号：US14547327
申请日：2014-11-19
申请人： BASF PLANT SCIENCE GMBH
发明人： Heiko A. Haertel , Garima Bhatt
IPC分类号： C07K14/415 , C12P7/64 , C12N15/82 , C07K16/16
CPC分类号： C07K14/415 , C07K16/16 , C12N9/00 , C12N9/1051 , C12N9/1205 , C12N9/93 , C12N15/8234 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12P7/6409
摘要： This invention relates generally to nucleic acid sequences encoding proteins that are related to the presence of seed storage compounds in plants. More specifically, the present invention relates to Arabidopsis thaliana, Brassica napus, Glycine max and Oryza sativa nucleic acid sequences encoding sugar and lipid metabolism regulator proteins and the use of these sequences in transgenic plants. In particular, the invention is directed to methods fir manipulating sugar-related compounds and for increasing oil level and altering the fatty acid composition in plants and seeds. The invention further relates to methods of using these novel plant polypeptides to stimulate plant growth and/or to increase yield and/or composition of seed storage compounds.
摘要翻译：本发明一般涉及编码与种子储存化合物在植物中的存在有关的蛋白质的核酸序列。更具体地，本发明涉及编码糖和脂质代谢调节蛋白的拟南芥，甘蓝型油菜，甘氨酸和水稻核酸序列，以及这些序列在转基因植物中的用途。特别地，本发明涉及冷冻操作糖相关化合物和提高油水平和改变植物和种子中的脂肪酸组成的方法。本发明还涉及使用这些新型植物多肽刺激植物生长和/或增加种子储存化合物的产量和/或组成的方法。

30. 发明申请

US20150033409A1 EXPRESSION CASSETTES FOR REGULATION OF EXPRESSION IN MONOCOTYLEDONOUS PLANTS 审中-公开
标题翻译：用于调控单克隆植物表达的表达体系
公开(公告)号：US20150033409A1
公开(公告)日：2015-01-29
申请号：US14505813
申请日：2014-10-03
申请人： BASF Plant Science GmbH
发明人： Hee-Sook Song , Marc Morra , Christian Dammann , Christina E. Roche , Effie Toren , Alleson Dobson
IPC分类号： C12N15/82 , C12Q1/68
CPC分类号： C12N15/8216 , C12N15/8205 , C12N15/8218 , C12N15/8225 , C12N15/8227 , C12N15/8234 , C12N15/8237 , C12N15/8261 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158
摘要： The present invention relates to expression cassettes comprising at least one transcription regulating nucleotide sequence obtainable from the group of genes of monocotyledonous plants consisting of caffeoyl-CoA-O-methyltransferase genes, C8,7-sterol isomerase genes, hydroxyproline-rich glycoprotein (HRGP) genes, lactate dehydrogenase genes, and chloroplast protein 12 like genes. More preferably the transcription regulating sequences are obtainable from Zea mays or Oryza sativa. The transcription regulating sequences are especially useful for root/kernel-preferential, leaf/endosperm-preferential, root/silk/kernel-preferential, or constitutive expression.
摘要翻译：本发明涉及包含至少一种转录调节核苷酸序列的表达盒，所述转录调节核苷酸序列可从由咖啡酰-CoA-O-甲基转移酶基因，C8,7-甾醇异构酶基因，富脯氨酸糖蛋白（HRGP）组成的单子叶植物的基因组获得，基因，乳酸脱氢酶基因和叶绿体蛋白12样基因。更优选地，转录调节序列可从玉米或水稻获得。转录调控序列特别适用于根/核优先，叶/胚乳优先，根/丝/核 - 优先或组成型表达。

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