专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

11. 发明申请

US20040043458A1 Coryneform bacteria which produce chemical compounds II 审中-公开
标题翻译：产生化学化合物的棒状细菌II
公开(公告)号：US20040043458A1
公开(公告)日：2004-03-04
申请号：US10358393
申请日：2003-02-05
申请人： Degussa AG
发明人： Brigitte Bathe , Caroline Kreutzer , Bettina Mockel , Georg Thierbach
IPC分类号： C12P019/34 , C12P013/08 , C12N001/21 , C12P025/00 , C12P023/00 , C12N015/74
CPC分类号： C12P13/08
摘要： The invention relates to coryneform bacteria which, instead of the singular copy of an open reading frame (ORF), gene or allele naturally present at the particular desired site (locus), have at least two copies of the open reading frame (ORF), gene or allele in question, preferably in tandem arrangement, and optionally at least a third copy of the open reading frame (ORF), gene or allele in question at a further gene site, and processes for the preparation of chemical compounds by fermentation of these bacteria.
摘要翻译：本发明涉及棒状细菌，代替开放阅读框（ORF）的单一拷贝，天然存在于特定所需位点（基因座）的基因或等位基因具有至少两个开放阅读框（ORF）的拷贝，基因或等位基因，优选串联排列，以及任选地在另一基因位点处的开放阅读框（ORF），所述基因或等位基因的至少第三拷贝，以及通过这些发酵制备化合物的方法菌。

12. 发明申请

US20030175854A1 System and method for gene expression in thermus strains 审中-公开
标题翻译：热应变株中基因表达的系统和方法
公开(公告)号：US20030175854A1
公开(公告)日：2003-09-18
申请号：US10086105
申请日：2002-02-28
发明人： Kevin J. Kayser , John J. Kilbane II
IPC分类号： C12P023/00 , C12N001/21 , C12N015/74
CPC分类号： C12N15/74 , C12P23/00
摘要： A method for producing beta-carotene and carotene-related pigments in which a plurality of thermophilic microorganisms is collected and screened for the production of pigments. Those pigment-producing thermophilic microorganisms having yellow, red or orange coloration are identified and separated from the collection of thermophilic microorganisms. Thereafter, the selected pigment-producing thermophilic microorganisms are mutated by non-recombinant means to enhance pigment production, forming a mutant pigment-producing thermophilic microorganism. In accordance with one embodiment of this invention, a gene of interest suitable for producing a protein of interest is introduced into the mutant pigment-producing thermophilic microorganism, resulting in over-production of the carotene pigment and the protein of interest. Also disclosed are suitable plasmids and expression vectors suitable for use in the method of this invention.
摘要翻译：一种生产β-胡萝卜素和胡萝卜素相关色素的方法，其中多个嗜热微生物被收集并筛选用于制备颜料。识别出具有黄色，红色或橙色着色的产生色素的嗜热微生物，并从嗜热微生物的收集中分离。此后，选择的产生色素的嗜热微生物通过非重组方法突变以增强颜料产生，形成产生突变色素的嗜热微生物。根据本发明的一个实施方案，将适合于产生感兴趣的蛋白质的目的基因引入产生突变色素的嗜热微生物中，导致胡萝卜素颜料和目的蛋白质的过量产生。还公开了适用于本发明方法的合适的质粒和表达载体。

13. 发明申请

US20030157592A1 Moss genes from physcomitrella patens encoding proteins involved in the synthesis of tocopherols and carotenoids 审中-公开
标题翻译：编码参与合成生育酚和类胡萝卜素的蛋白质的小麦幼虫的苔藓基因
公开(公告)号：US20030157592A1
公开(公告)日：2003-08-21
申请号：US10149759
申请日：2002-06-13
发明人： Jens Lerchl , Andreas Renz , Thomas Ehrhardt , Andreas Reindl , Petra Cirpus , Friedrich Bischoff , Markus Frank , Annette Freund , Elke Duwenig , Ralf-Michael Schmidt , Ralf Reski , Ralf Badur
IPC分类号： C12P023/00 , C12N009/10 , A01H001/00 , C12N015/82 , C12P021/02 , C12N005/04
CPC分类号： C12N9/1007 , C12N15/52 , C12N15/8243 , C12N15/825
摘要： Isolated nucleic acid molecules, designated TCMRP nucleic acid molecules, which encode novel TCMRPs from e.g. Phycomitrella patens are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing TCMRP nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated TCMRPs, mutated TCMRPs, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from transformed cells, organisms or plants based on genetic engineering of TCMRP genes in these organisms.
摘要翻译：分离的核酸分子，命名为TCMRP核酸分子，其编码来自例如新鲜的TCMRP。描述了伞菌属（Phycomitrella patens）。本发明还提供反义核酸分子，含有TCMRP核酸分子的重组表达载体，以及引入了表达载体的宿主细胞。本发明还进一步提供了分离的TCMRP，突变的TCMRP，融合蛋白，抗原肽以及基于这些生物体中TCMRP基因的遗传工程改进从转化细胞，生物体或植物生产所需化合物的方法。

14. 发明申请

US20030143659A1 PROCESS FOR THE PREPARATION OF A GRANULAR MICROBIAL BIOMASS AND ISOLATION OF A COMPOUND THEREFORM 无效
标题翻译：制备颗粒微生物生物量的方法及其化合物的分离
公开(公告)号：US20030143659A1
公开(公告)日：2003-07-31
申请号：US08821025
申请日：1997-03-19
发明人： HENDRIK LOUIS BIJL , ALBERT SCHAAP
IPC分类号： C12P023/00 , C12N001/00
CPC分类号： C11B3/006 , A23D9/00 , A23K20/158 , A23L5/00 , A23L31/00 , A23L31/10 , A23L33/11 , A23L33/12 , A23L33/15 , A23V2002/00 , A61K8/375 , A61K8/67 , A61K8/92 , A61K8/9706 , A61K8/99 , A61K2800/85 , A61Q19/00 , C07C51/47 , C11B1/02 , C11B1/10 , C12N1/005 , C12N1/14 , C12P7/6427 , C12P7/6472 , C12P17/06 , C12P19/42 , C12P23/00 , C07C57/03 , C07C57/12 , A23V2250/706 , A23V2250/1868
摘要： A process for the isolation of desired compound(s) from a microbial biomass is disclosed, wherein the microbial biomass (which, if necessary, is pretreated to give a dry matter content of from 25 to 80%) is granulated (e.g. by extrusion) and then dried to a dry matter content of at least 80%. The granulation of the biomass to granules significantly eases subsequent drying of the biomass (which can be stored as dried granules) and gives higher yields on extraction of the compound(s).
摘要翻译：公开了从微生物生物质中分离所需化合物的方法，其中微生物生物质（如果需要，预处理以产生25-80％的干物质含量）被造粒（例如通过挤出）然后干燥至干物质含量至少为80％。生物质颗粒的造粒可显着缓解生物质的后续干燥（可以以干燥颗粒的形式储存），并提高化合物的产率。

15. 发明申请

US20030087336A1 Beta, beta-carotene 15, 15'-monooxygenases, nucleic acid sequences coding therefor and their use 失效
标题翻译： β，β-胡萝卜素15,15'-单加氧酶，对其编码的核酸序列及其用途
公开(公告)号：US20030087336A1
公开(公告)日：2003-05-08
申请号：US10053192
申请日：2002-01-15
发明人： Heinrich Bachmann , Roland Brugger , Arno Martin Friedlein , Gabriele Margarethe Wirtz , Wolf-Dietrich Woggon , Adrian Wyss , Markus Wyss
IPC分类号： C12P023/00 , C12P021/02 , C12N005/06 , C12N009/04 , C07H021/04 , A23L001/32
CPC分类号： C12P23/00 , C12N9/0069
摘要： Nucleic acid sequences coding for a protein having null,null-carotene 15,15null-monooxygenase activity and their uses in diagnostics, the synthesis of vitamin A and methods for the introduction of the null,null-carotene 15,15null-monooxygenase cDNA into host cells are disclosed.
摘要翻译：编码具有β，β-胡萝卜素15,15'-单加氧酶活性的蛋白质的核酸序列及其在诊断中的应用，维生素A的合成以及引入β，β-胡萝卜素15,15'-单加氧酶cDNA的方法公开了宿主细胞。

16. 发明申请

US20020177181A1 Increasing bioavailability of carotenoids 审中-公开
标题翻译：增加类胡萝卜素的生物利用度
公开(公告)号：US20020177181A1
公开(公告)日：2002-11-28
申请号：US09915527
申请日：2001-07-27
申请人： Agricultural Research Organization, The Volcani Center
发明人： Joseph Kanner , Arieh Levy , Rina Granit
IPC分类号： C12Q001/44 , C12P023/00
CPC分类号： C12Q1/44 , A23K20/179 , A23L5/44 , A23L33/105 , A23L33/12 , A23V2002/00 , C12P23/00 , A23V2250/211 , A23V2250/186
摘要： A method of increasing a fraction of free carotenoids in a source of carotenoids in which at least some of the carotenoids are fatty acid esterified carotenoids is disclosed. The method is effected by contacting the source of carotenoids with an effective amount of an esterase under conditions effective in deesterifying the fatty acid esterified carotenoids, thereby increasing the fraction of free carotenoids in the source of carotenoids.
摘要翻译：公开了一种在类胡萝卜素源中增加游离类胡萝卜素的一部分的方法，其中至少一些类胡萝卜素是脂肪酸酯化的类胡萝卜素。该方法通过在有效地酯化脂肪酸酯化的类胡萝卜素的条件下将类胡萝卜素源与有效量的酯酶接触，从而增加类胡萝卜素来源中的游离类胡萝卜素的分数。

17. 发明申请

US20020053096A1 Polynucleotide molecule from Haematococcus pluvialis encoding a polypeptide having a beta-C-4-oxygenase activity for biotechnological production of (3S,3'S) astaxanthin and its specific expression in chromoplasts of higher plants 失效
标题翻译：（3S，3'S）虾青素生物技术生产中具有β-C-4-加氧酶活性的多肽的多核苷酸分子及其在高等植物色素体中的特异性表达
公开(公告)号：US20020053096A1
公开(公告)日：2002-05-02
申请号：US09791687
申请日：2001-02-26
发明人： Joseph Hirschberg , Tamar Lotan
IPC分类号： A01H005/00 , C07H021/04 , C12P023/00 , C12N001/21
CPC分类号： C12N9/0071 , C12N15/825
摘要： The present invention relates, in general, to a biotechnological method for production of (3S,3nullS) astaxanthin. In particular, the present invention relates to a peptide having a null-C-4-oxygenase activity; a DNA segment coding for this peptide; an RNA segments coding for this peptide; a recombinant DNA molecule comprising a vector and the DNA segment; a host cell or organism containing the above described recombinant DNA molecule or DNA segment; and to a method of biotechnologically producing (3S,3nullS) astaxanthin or a food additive containing (3S,3nullS) astaxanthin, using the host.
摘要翻译：本发明一般涉及生产（3S，3'S）虾青素的生物技术方法。特别地，本发明涉及具有β-C-4-加氧酶活性的肽; 编码该肽的DNA区段; 编码该肽的RNA片段; 包含载体和DNA片段的重组DNA分子; 含有上述重组DNA分子或DNA片段的宿主细胞或生物体; 以及使用该主体的生物技术生产（3S，3'S）虾青素或含有（3S，3'S）虾青素的食品添加剂的方法。

18. 发明申请

US20040219629A1 Increasing carotenoid production in bacteria via chromosomal integration 审中-公开
标题翻译：通过染色体整合增加细菌中的类胡萝卜素生成
公开(公告)号：US20040219629A1
公开(公告)日：2004-11-04
申请号：US10735442
申请日：2003-12-12
发明人： Qiong Cheng , Pierre E. Rouviere , Wonchul Suh
IPC分类号： C12P023/00 , C12N001/21
CPC分类号： C12P23/00
摘要： The present invention relates to carotenoid overproducing bacteria The genes of the isoprenoid pathway in the bacterial hosts of the invention have been engineered such that certain genes are either up-regulated or down regulated resulting in the production of carotenoid compounds at a higher level than is found in the un-modified host. Genes that may be up-regulated include the dxs, idi, ispB, lytB and ygbBP genes. Additionally it has been found that a partial disruption of the yjeR gene has the effect of enhancing carotenoid production.
摘要翻译：本发明涉及类胡萝卜素过量生产细菌本发明的细菌宿主中的类异戊二烯途径的基因已被设计成使得某些基因被上调或下调，导致类胡萝卜素化合物的产生高于所发现的水平在未修改的主机中。可能上调的基因包括dxs，idi，ispB，lytB和ygbBP基因。另外还发现yjeR基因的部分破坏具有增强类胡萝卜素生成的作用。

19. 发明申请

US20040146966A1 Mutations affecting carotenoid production 失效
标题翻译：影响类胡萝卜素生产的突变
公开(公告)号：US20040146966A1
公开(公告)日：2004-07-29
申请号：US10735008
申请日：2003-12-12
发明人： Qiong Cheng , Pierre E. Rouviere , Luan Tao
IPC分类号： C12P023/00 , C12N001/21 , C12N001/16 , C12N001/18
CPC分类号： C12P23/00 , C07K14/24 , C12N15/52
摘要： Mutations in genes having no direct relationship to the carotenoid biosynthetic pathway have been found to increase carbon flux through that pathway. Complete disruption in the deaD, mreC, and yfhE genes were effective. Additionally where genes of the lower carotenoid pathway reside on a plasmid having either a p15A or pMB1 replicon, mutations in the thrS, rspA, rpoC, yjeR, and rhoL were found effective.
摘要翻译：已经发现与类胡萝卜素生物合成途径没有直接关系的基因中的突变增加通过该途径的碳通量。 deaD，mreC和yfhE基因的完全破坏是有效的。此外，较低类胡萝卜素途径的基因存在于具有p15A或pMB1复制子的质粒上，发现thrS，rspA，rpoC，yjeR和rhoL中的突变是有效的。

20. 发明申请

US20040067550A1 Method for the production of beta-carotene 有权
标题翻译：生产β-胡萝卜素的方法
公开(公告)号：US20040067550A1
公开(公告)日：2004-04-08
申请号：US10333331
申请日：2003-11-06
发明人： Javier Costa Perez , Antonio Estrella Castro , Ana Teresa Marcos Rodriguez , J Emiliano Gonzalez De Prado , Enrique R Peiro Cezon , Alfonso Collados De La Vieja , Manuel Esteban Morales
IPC分类号： C12P023/00
CPC分类号： A23L5/44 , C09B61/00 , C12P23/00 , Y10S435/911 , Y10S435/931
摘要： The invention relates to a novel method for the production of null-carotene from submerged cultures of mucoral fungi such as Blakeslea, Choanephora or Phycomyces by adding lectin to the culture medium and performing pH control once fermentation has started. The method involves null-carotene recovery stage that makes it possible to simplify the process, optimize yields and increase purification of the product.
摘要翻译：本发明涉及通过向培养基中加入凝血素并一旦发酵开始进行pH控制，从粘液真菌例如布鲁氏菌（Blakeslea），Cho eph属（Choanephora）或Phycomyces等浸没培养物中生产β-胡萝卜素的新方法。该方法包括β-胡萝卜素回收阶段，可以简化工艺，优化产量并增加产品的纯化。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式