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11. 发明申请

US20180284009A1 APPARATUSES, SYSTEMS AND METHODS FOR IMAGING FLOW CYTOMETRY 审中-公开
公开(公告)号：US20180284009A1
公开(公告)日：2018-10-04
申请号：US15941791
申请日：2018-03-30
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： Gregory KADUCHAK , Michael WARD
IPC分类号： G01N15/14 , G01N21/53 , G01N15/02 , G01N21/64 , G01J3/44
CPC分类号： G01N15/1436 , B01L3/502761 , B01L2200/0652 , B01L2400/082 , G01J3/4406 , G01N15/0227 , G01N15/0272 , G01N15/1404 , G01N15/1429 , G01N15/1434 , G01N15/1456 , G01N15/1459 , G01N15/1468 , G01N15/147 , G01N21/532 , G01N21/6456 , G01N2015/0003 , G01N2015/0011 , G01N2015/03 , G01N2015/1006 , G01N2015/1081 , G01N2015/1415 , G01N2015/142 , G01N2015/1422 , G01N2015/1438 , G01N2015/1445 , G01N2015/1452 , G01N2015/149
摘要： The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

12. 发明申请

US20170354973A1 METHOD AND DEVICE FOR TRACKING AND MANIPULATION OF DROPLETS 审中-公开
公开(公告)号：US20170354973A1
公开(公告)日：2017-12-14
申请号：US15521231
申请日：2015-10-26
申请人： Sandia Corporation
发明人： Jess M. Sustarich , Chao Chung Shih , Anup Kumar Singh , Philip Gach
IPC分类号： B01L3/00 , C12N15/10 , B01L7/00 , G01N15/14 , B01F13/00
CPC分类号： B01L3/502784 , B01F13/0071 , B01F13/0076 , B01F2215/0037 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L7/00 , B01L2200/0605 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2300/0867 , B01L2300/0883 , B01L2300/1805 , B01L2400/0415 , B01L2400/0424 , B01L2400/082 , B01L2400/086 , C12N15/1058 , G01N15/1404 , G01N15/1434 , G01N35/08 , G01N2015/1413 , G01N2015/1422 , G01N2015/149
摘要： Disclosed are devices and methods useful for confined-channel digital microfluidics that combine high-throughput droplet generators with digital microfluidic for droplet manipulation. The present disclosure also provides an off-chip sensing system for droplet tracking.

13. 发明申请

US20160161391A1 NOZZLE AND METHOD FOR FLOW CYTOMETRY 审中-公开
标题翻译：用于流动细胞计数的喷嘴和方法
公开(公告)号：US20160161391A1
公开(公告)日：2016-06-09
申请号：US14888906
申请日：2014-05-08
申请人： Detlef RATH , MASTERRIND GMBH
发明人： Detlef Rath
IPC分类号： G01N15/14 , C12M1/00 , G01N33/487
CPC分类号： G01N15/1404 , C12M47/04 , G01N15/14 , G01N33/487 , G01N2015/1006 , G01N2015/1081 , G01N2015/1236 , G01N2015/1409 , G01N2015/1413 , G01N2015/1422 , G01N2015/149
摘要： The invention relates to a nozzle for flow cytometry, the housing of which is tapering towards an outlet and in which a feed tube is arranged for a core flow liquid, the outlet opening of which is arranged at a distance from the outlet of the housing. The outlet of the housing forms the outlet of the nozzle. The housing of the nozzle extends from its outlet, which is arranged at its first end to its opposite second end, and has an inlet for a sheath flow liquid connected with the internal volume. The nozzle is characterized in that in the housing a leading element that promotes the alignment of particles extends from both sides of the feed tube.
摘要翻译：本发明涉及一种用于流式细胞术的喷嘴，其壳体朝向出口逐渐变细，并且其中为芯流动液体设置进料管，其出口开口设置在离壳体出口一定距离处。外壳的出口形成喷嘴的出口。喷嘴的壳体从其出口延伸，其出口在其第一端设置到其相对的第二端，并具有用于与内部容积连接的护套流动液体的入口。喷嘴的特征在于，在壳体中，促进颗粒对准的引导元件从进料管的两侧延伸。

14. 发明申请

US20140246599A1 System and Apparatus to Illuminate Individual Particles 审中-公开
标题翻译：照亮单个颗粒的系统和装置
公开(公告)号：US20140246599A1
公开(公告)日：2014-09-04
申请号：US14082507
申请日：2013-11-18
申请人： Matthew B Hart , Patrick M Nolan , Jay D Eversole
发明人： Matthew B Hart , Patrick M Nolan , Jay D Eversole
IPC分类号： H01J49/02
CPC分类号： G01N15/1404 , G01N21/33 , G01N2015/1422 , G01N2015/149 , H01J49/422
摘要： An apparatus for illuminating individual particles comprising a device for moving and directing air containing particles into a system, the system comprising an electrodynamic linear quadrupole section, an ultra-violet electromagnetic radiation source located along the electrodynamic linear quadrupole section, and a collection device for collecting the particles. A method of illuminating individual particles comprising moving and directing air containing particles into a system, controlling the air flow by using an air pump that continuously pulls or pushes air through the system, directing the particles into an electrodynamic linear quadrupole section, confining the particles to the central axis of the electrodynamic linear quadrupole section, illuminating the particles with ultra-violet electromagnetic radiation, interrogating the particles, and collecting the particles.
摘要翻译：一种用于照射单个颗粒的装置，包括用于将含有颗粒的空气移动并引导到系统中的装置，该系统包括电动力线性四极段，沿着电动线性四极杆部分设置的紫外线电磁辐射源，以及用于收集颗粒。一种照射单个颗粒的方法，包括将含有颗粒的空气移动并引导到系统中，通过使用连续拉动或推动空气通过系统的空气泵来控制空气流，将颗粒引导到电动线性四极杆段中，将颗粒限制在电动线性四极节段的中心轴线，用紫外线电磁辐射照射粒子，询问粒子，并收集粒子。

15. 发明授权

US09588036B2 Microchip for sorting micro particles and cartridge including same 有权
标题翻译：微芯片用于分选微粒和包括它的墨盒
公开(公告)号：US09588036B2
公开(公告)日：2017-03-07
申请号：US14957072
申请日：2015-12-02
申请人： Sony Corporation
发明人： Masataka Shinoda
IPC分类号： G01N27/453 , G01N15/14 , B01L3/02 , G01N27/447 , B01L3/00 , G01N15/10
CPC分类号： G01N15/1404 , B01L3/0268 , B01L3/502746 , B01L3/502761 , B01L3/502776 , B01L2400/0433 , B01L2400/0487 , G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N27/44743 , G01N27/44791 , G01N2015/1006 , G01N2015/1081 , G01N2015/1409 , G01N2015/1422 , G01N2015/1481 , G01N2015/1488 , G01N2015/149 , Y10T137/8158
摘要： A microchip is provided that includes a flow path through which a liquid containing a micro particle flows, an orifice through which the liquid flowing through the flow path is discharged into a space outside the microchip, and a light-irradiated portion provided at a predetermined location of the flow path and configured to be irradiated with light. A width of the flow path and a depth of the flow path at the orifice are set to be smaller than a width of the flow path and a depth of the flow path at the light-irradiated portion, and the flow path is configured to gradually decrease from upstream of the orifice in a cross-section area perpendicular to a liquid-delivering direction between the light-irradiated portion and the orifice. A cartridge including the microchip is also provided.
摘要翻译：提供了一种微芯片，其包括流过微流体的液体流过的流路，流过流路的液体通过该流路排出到微芯片外的空间中，以及设置在预定位置的光照射部的流路并被配置成用光照射。流路的宽度和孔口处的流路的深度被设定为小于流路的宽度和在光照射部分处的流路的深度，并且流路被构造成逐渐在与光照射部分和孔之间的液体输送方向垂直的截面区域中从孔的上游减小。还提供了包括微芯片的盒。

16. 发明授权

US08795500B2 Apparatus and microchip for sorting micro particles 有权
标题翻译：用于分选微粒的装置和微芯片
公开(公告)号：US08795500B2
公开(公告)日：2014-08-05
申请号：US13147517
申请日：2010-02-09
申请人： Masataka Shinoda
发明人： Masataka Shinoda
IPC分类号： G01N27/403 , B03C5/00
CPC分类号： G01N15/1404 , B01L3/0268 , B01L3/502746 , B01L3/502761 , B01L3/502776 , B01L2400/0433 , B01L2400/0487 , G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N27/44743 , G01N27/44791 , G01N2015/1006 , G01N2015/1081 , G01N2015/1409 , G01N2015/1422 , G01N2015/1481 , G01N2015/1488 , G01N2015/149 , Y10T137/8158
摘要： In one example embodiment, a micro-particle sorting apparatus includes: (a) a microchip in which a flow path through which liquid containing a micro particle flows and an orifice through which the liquid flowing through the flow path is discharged into a space outside the chip; (b) an oscillating element for transforming the liquid into a liquid drop and discharging the liquid drop at the orifice; (c) a charge means for adding an electric charge to the discharged liquid drop; (d) an optical detection means that detects an optical property of the micro particle flowing through the flow path, upstream of a liquid-delivering direction with respect to the orifice; (e) paired electrodes opposed to each other while sandwiching the moving liquid drop along a movement direction of the liquid drop discharged into the space outside the chip; and (f) two containers that collect the liquid drop passing between the paired electrodes.
摘要翻译：在一个示例性实施例中，微粒子分选装置包括：（a）微芯片，其中包含微粒子的液体流过的流路和流过流路的液体通过该孔流入外部的空间芯片; （b）用于将液体转换成液滴并在孔口处排出液滴的振荡元件; （c）用于向排出的液滴添加电荷的充电装置; （d）光检测装置，其检测流过所述流路的微粒子相对于所述孔口在液体输送方向上游的光学特性; （e）沿着排出到芯片外部的空间的液滴的移动方向夹着移动的液滴，彼此相对的成对电极; 和（f）两个收集通过成对电极之间的液滴的容器。

17. 发明申请

US20130220811A1 METHOD AND APPARATUS FOR CONTROLLING A FLOW OF PARTICLES BY WAY OF A THROUGH-HOLE 有权
标题翻译：用于通过孔的方式控制颗粒流的方法和装置
公开(公告)号：US20130220811A1
公开(公告)日：2013-08-29
申请号：US13773863
申请日：2013-02-22
申请人： The Board of Trustees of The University of Illinois
发明人： Aleksei Aksimentiev
IPC分类号： G01N27/447
CPC分类号： G01N27/447 , G01N15/1404 , G01N27/44791 , G01N33/48721 , G01N2015/1422 , Y10T137/7722
摘要： A system that incorporates the subject disclosure may include, for example, a method for generating an electric or pressure difference force that induces a plurality of particles to flow through a through-hole. Independently adjustable heat source in a vicinity of the through-hole induces a thermodynamic force for modifying the flow of the plurality of particles through the through-hole. Additional embodiments are disclosed.
摘要翻译：结合本公开的系统可以包括例如用于产生诱发多个粒子流过通孔的电力或压力差力的方法。在通孔附近的独立可调的热源引起用于改变多个颗粒通过通孔的流动的热力。公开了另外的实施例。

18. 发明申请

US20110284378A1 APPARATUS AND MICROCHIP FOR SORTING MICRO PARTICLES 有权
标题翻译：用于分选微粒的装置和微型计算机
公开(公告)号：US20110284378A1
公开(公告)日：2011-11-24
申请号：US13147517
申请日：2010-02-09
申请人： Masataka Shinoda
发明人： Masataka Shinoda
IPC分类号： G01N15/14 , F15D1/00 , G01N27/447
CPC分类号： G01N15/1404 , B01L3/0268 , B01L3/502746 , B01L3/502761 , B01L3/502776 , B01L2400/0433 , B01L2400/0487 , G01N15/1434 , G01N15/1459 , G01N15/1484 , G01N27/44743 , G01N27/44791 , G01N2015/1006 , G01N2015/1081 , G01N2015/1409 , G01N2015/1422 , G01N2015/1481 , G01N2015/1488 , G01N2015/149 , Y10T137/8158
摘要： In one example embodiment, a micro-particle sorting apparatus includes: (a) a microchip in which a flow path through which liquid containing a micro particle flows and an orifice through which the liquid flowing through the flow path is discharged into a space outside the chip; (b) an oscillating element for transforming the liquid into a liquid drop and discharging the liquid drop at the orifice; (c) a charge means for adding an electric charge to the discharged liquid drop; (d) an optical detection means that detects an optical property of the micro particle flowing through the flow path, upstream of a liquid-delivering direction with respect to the orifice; (e) paired electrodes opposed to each other while sandwiching the moving liquid drop along a movement direction of the liquid drop discharged into the space outside the chip; and two containers that collect the liquid drop passing between the paired electrodes.
摘要翻译：在一个示例性实施例中，微粒子分选装置包括：（a）微芯片，其中包含微粒子的液体流过的流路和流过流路的液体通过该孔流入外部的空间芯片; （b）用于将液体转换成液滴并在孔口处排出液滴的振荡元件; （c）用于向排出的液滴添加电荷的充电装置; （d）光检测装置，其检测流过所述流路的微粒子相对于所述孔口在液体输送方向上游的光学特性; （e）沿着排出到芯片外部的空间的液滴的移动方向夹着移动的液滴，彼此相对的成对电极; 以及两个收集通过成对电极之间的液滴的容器。

19. 发明申请

US20170291172A1 TUNABLE, SHEATHLESS, AND THREE DIMENSIONAL SINGLE-STREAM CELL FOCUSING AND SORTING IN HIGH SPEED FLOWS 审中-公开
公开(公告)号：US20170291172A1
公开(公告)日：2017-10-12
申请号：US15484964
申请日：2017-04-11
申请人： The Regents of the University of California
发明人： YU-CHUN KUNG , PEI-YU E. CHIOU
IPC分类号： B01L3/00 , G01N27/447 , G01N1/10 , F04B43/04 , F16K99/00
CPC分类号： G01N27/44791 , B01L3/502707 , B01L3/502761 , B01L2200/0652 , B01L2400/0424 , F04B43/043 , G01N1/10 , G01N15/1404 , G01N2015/1006 , G01N2015/1422
摘要： In various embodiments methods and devices are provided for focusing and/or sorting particles and/or cells in a microfluidic channel. In certain embodiments the device comprises a microfluidic channel comprising a plurality of electrodes disposed to provide dielectrophoretic (DEP) forces that are perpendicular to hydrodynamic flows along the channel; wherein said device is configured to apply voltages to said electrodes to provide an electric field minimum that is not centered in said microfluidic channel.

20. 发明授权

US09638661B2 Method and apparatus for controlling a flow of particles by way of a through-hole 有权
公开(公告)号：US09638661B2
公开(公告)日：2017-05-02
申请号：US13773863
申请日：2013-02-22
申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
发明人： Aleksei Aksimentiev
IPC分类号： G01N27/447 , G01N33/487 , G01N15/14
CPC分类号： G01N27/447 , G01N15/1404 , G01N27/44791 , G01N33/48721 , G01N2015/1422 , Y10T137/7722
摘要： A system that incorporates the subject disclosure may include, for example, a method for generating an electric or pressure difference force that induces a plurality of particles to flow through a through-hole. Independently adjustable heat source in a vicinity of the through-hole induces a thermodynamic force for modifying the flow of the plurality of particles through the through-hole. Additional embodiments are disclosed.

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