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11. 发明申请

US20090053359A1 AMMONIA TRANSPORTER GENE AND USE THEREOF 审中-公开
标题翻译：氨基酸运输基因及其使用
公开(公告)号：US20090053359A1
公开(公告)日：2009-02-26
申请号：US12279080
申请日：2006-12-05
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12G1/00 , C07H21/00 , C07K14/00 , C12N15/00 , C12N15/63 , C12N1/19 , C12Q1/02 , C12Q1/68 , C12C11/00
CPC分类号： C07K14/435 , C12C12/004 , C12C12/006 , C12G1/0203
摘要： The present invention relates to ammonia transporter genes and use thereof. The invention relates in particular to a brewer's yeast which shows enhanced ammonia assimilation, alcoholic beverages produced using such yeast, and a method of producing such alcoholic beverages. More specifically, the invention relates to the MEP3 gene which codes for the ammonia transporter Mep3 in brewer's yeast, particularly to a yeast which can control the ammonia assimilation ability by controlling the level of expression of the nonScMEP3 gene or ScMEP3 gene characteristic to beer yeast and to a method of producing alcoholic beverages using such yeast.
摘要翻译：本发明涉及氨转运蛋白基因及其应用。本发明特别涉及显示增强的氨同化的酿酒酵母，使用这种酵母生产的含酒精饮料，以及生产这种酒精饮料的方法。更具体地，本发明涉及编码啤酒酵母中氨转运蛋白Mep3的MEP3基因，特别是涉及通过控制啤酒酵母特有的nonScMEP3基因或ScMEP3基因的表达水平来控制氨同化能力的酵母，涉及使用这种酵母生产酒精饮料的方法。

12. 发明申请

US20090130255A1 Catalase Gene and Use Thereof 审中-公开
标题翻译：过氧化氢酶基因及其用途
公开(公告)号：US20090130255A1
公开(公告)日：2009-05-21
申请号：US11919226
申请日：2006-12-22
申请人： Yoshihiro Nakao , Yukiko Kadama , Tomoko Shimonaga , Fumihiko Omura
发明人： Yoshihiro Nakao , Yukiko Kadama , Tomoko Shimonaga , Fumihiko Omura
IPC分类号： C12C11/00 , C12G1/00 , C12P21/06 , C12N9/02 , C12N1/18 , C07H21/04
CPC分类号： C12C12/006 , C12C12/004 , C12G1/0203 , C12N9/0065 , C12Y111/01006
摘要： The present invention relates to a gene encoding a catalase and use thereof, in particular, a brewery yeast having high sulfite-producing capability, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose capability of producing sulfite, that contribute to stability of flavor in products, is enhanced by amplifying expression level of CTT1 gene encoding a catalase Ctt1p, especially non-ScCTT1 gene or ScCTT1 gene specific to a lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.
摘要翻译：本发明涉及编码过氧化氢酶的基因及其用途，特别是具有高亚硫酸盐生产能力的啤酒酵母，用该酵母生产的酒精饮料及其制造方法。更具体地，本发明涉及通过扩增编码过氧化氢酶Ctt1p的CTT1基因，特别是非ScCTT1基因或ScCTT1基因特异性的ScCTT1基因的表达水平来提高其产生亚硫酸盐的能力，其有助于产品中风味稳定性的酵母啤酒酿造酵母，以及用所述酵母生产酒精饮料的方法。

13. 发明申请

US20100221383A1 GENE ENCODING GLYCOGEN BRANCHING ENZYME AND USE THEREOF 审中-公开
标题翻译：基因编码GLYCOGEN分支酶及其使用
公开(公告)号：US20100221383A1
公开(公告)日：2010-09-02
申请号：US12279610
申请日：2007-02-01
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12N1/19 , C07H21/04 , C12N9/10 , C12N15/74 , C12Q1/68 , G01N33/53 , C12C11/00 , C12G1/00
CPC分类号： C12N9/107 , C12N1/04
摘要： The present invention relates to a gene encoding glycogen branching enzyme and use thereof, in particular, a yeast for practical use with superior resistance property to dryness and/or low-temperature storage, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose resistance property to dryness and/or resistance property to low-temperature storage is enhanced by amplifying expression level of GLC3 gene encoding a glycogen branching enzyme Glc3p in brewer's yeast, especially non-ScGLC3 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.
摘要翻译：本发明涉及编码糖原分解酶的基因及其用途，特别是具有优异的耐干性和/或低温保存性的实际用途的酵母，所述酵母生产的酒精饮料及其制备方法饮料。更具体地说，本发明涉及一种通过扩增酿酒酵母，特别是非ScGLC3基因中编码糖原分支酶Glc3p的GLC3基因的表达水平来增强其耐低温性和/或抗低温贮藏性能的酵母特别适用于啤酒酿造酵母和用所述酵母等生产酒精饮料的方法

14. 发明申请

US20090041891A1 Gene Encoding Transcriptional Inducer for Maltase Gene and Maltose Transporter Gene and use Thereof 审中-公开
标题翻译：基因编码转录诱导因子的麦芽糖酶基因和麦芽糖转运蛋白基因及其用途
公开(公告)号：US20090041891A1
公开(公告)日：2009-02-12
申请号：US12280805
申请日：2007-02-21
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12C11/00 , C12N15/11 , C07K14/00 , C12Q1/68 , C12N15/00 , C12N1/19
CPC分类号： C07K14/395 , C12N9/2408
摘要： The present invention relates to a gene encoding a transcriptional inducer for maltase gene and maltose transporter gene and use thereof, in particular, a brewer's yeast with high maltose assimilation ability, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose maltose assimilation is enhanced by amplifying expression level of MALR gene encoding MalRp, a maltase and maltose transporter transcription factor in brewer's yeast, especially non-ScMALR gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.
摘要翻译：本发明涉及编码麦芽糖酶基因和麦芽糖转运蛋白基因的转录诱导物的基因及其用途，特别是具有高麦芽糖同化能力的啤酒酵母，用该酵母生产的酒精饮料及其制造方法。更具体地说，本发明涉及通过扩增酿酒酵母中的MalRp，麦芽糖酶和麦芽糖转运蛋白转录因子的MALR基因的表达水平，特别是对大啤酒酵母特异性的非ScMALR基因来增强其麦芽糖同化的酵母，用酵母等生产酒精饮料的方法

15. 发明申请

US20090029001A1 GENE ENCODING PROTEIN RESPONSIBLE FOR FLOCCULATION PROPERTY OF YEAST AND USE THEREOF 审中-公开
标题翻译：基因编码蛋白质对FOR ATION属性的影响及其用途
公开(公告)号：US20090029001A1
公开(公告)日：2009-01-29
申请号：US12279389
申请日：2006-12-27
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12G1/00 , C07H21/04 , C07H21/02 , C07K14/395 , C12Q1/68 , C12N15/63 , C12N1/19 , C12C1/00
CPC分类号： C07K14/395 , C12C12/004 , C12C12/006 , C12G1/0203
摘要： The present invention relates to a gene encoding a protein responsible for flocculation property of yeast and use thereof, in particular, a brewery yeast with appropriate flocculation property for producing desired alcoholic beverages, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast to which suitable flocculation property for brewing desired alcoholic beverages was imparted by controlling expression level of KRE9 gene encoding a protein responsible for flocculation property of brewery yeast, especially non-ScKRE9 gene specific to a lager brewing yeast, to alcoholic beverages produced with said yeast and to a method for producing said beverages.
摘要翻译：本发明涉及编码负责酵母的絮凝性的蛋白质及其用途的基因，特别是具有适当的絮凝性的酿造酵母，用于生产所需的酒精饮料，用所述酵母生产的酒精饮料，以及所述饮料的制造方法。更具体地，本发明涉及通过控制编码负责啤酒酵母的絮凝性质的蛋白质的KRE9基因的表达水平，特别是对大啤酒特异性的非ScKRE9基因来赋予酿造所需酒精饮料的合适的絮凝性能的酵母酵母，用所述酵母生产的酒精饮料和生产所述饮料的方法。

16. 发明申请

US20090017161A1 GENE ENCODING PROTEIN HAVING TREHALOSE SYNTHESIS-PROMOTING ACTIVITY AND USE THEREOF 审中-公开
标题翻译：基因编码蛋白质合成促进活性及其用途
公开(公告)号：US20090017161A1
公开(公告)日：2009-01-15
申请号：US12279206
申请日：2007-02-01
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12C11/00 , C12N15/11 , C07K14/00 , C12Q1/68 , C12N15/00 , C12N1/19
CPC分类号： C12C12/006 , C12C12/004 , C12G1/02 , C12N9/90
摘要： The present invention relates to a gene encoding a protein having a trehalose synthesis-promoting activity and use thereof, in particular, a yeast for practical use with superior resistance property to dryness and/or low-temperature storage, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose resistance property to dryness and/or resistance property to low-temperature storage is enhanced by amplifying expression level of TSL1 gene encoding a protein Ts11p having a trehalose synthesis-promoting activity in brewer's yeast, especially non-ScTSL1 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.
摘要翻译：本发明涉及编码具有海藻糖合成促进活性的蛋白质的基因及其用途，特别是具有优异的耐干性和/或低温保存性的实际应用的酵母，所述酵母生产的酒精饮料，以及生产所述饮料的方法。更具体地，本发明涉及通过扩增编码在啤酒酵母中具有海藻糖合成促进活性的蛋白质Ts11p的TSL1基因的表达水平来增强其对耐低温性和耐干燥性的耐性的酵母，特别是对于大型酿造酵母特异性的非scTSL1基因以及用所述酵母等生产酒精饮料的方法。

17. 发明申请

US20090011080A1 GENE ENCODING PROTEIN RESPONSIBLE FOR FLOCCULATION PROPERTY OF YEAST AND USE THEREOF 审中-公开
标题翻译：基因编码蛋白质的絮凝性质及其用途
公开(公告)号：US20090011080A1
公开(公告)日：2009-01-08
申请号：US12280584
申请日：2007-01-15
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12C11/00 , C07H21/04 , C07H21/02 , C07K14/395 , C12Q1/68 , C12Q1/02 , A23L1/185 , C12N15/63 , C12N1/19
CPC分类号： C07K14/395
摘要： The present invention relates to a gene encoding a protein responsible for flocculation property of yeast and use thereof, in particular, a brewery yeast with appropriate flocculation property for producing desired alcoholic beverages, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast to which suitable flocculation property for brewing desired alcoholic beverages was imparted by controlling expression level of CIS3 gene encoding a protein responsible for flocculation property of brewery yeast, especially non-ScCIS3 gene specific to a lager brewing yeast, to alcoholic beverages produced with said yeast and to a method for producing said beverages.
摘要翻译：本发明涉及编码负责酵母的絮凝性的蛋白质及其用途的基因，特别是具有适当的絮凝性的酿造酵母，用于生产所需的酒精饮料，用所述酵母生产的酒精饮料，以及所述饮料的制造方法。更具体地，本发明涉及通过控制编码负责啤酒酵母的絮凝特性的蛋白质的CIS3基因的表达水平，特别是对大啤酒特异性的非ScCIS3基因来赋予酿造所需酒精饮料的合适的絮凝特性的酵母酵母，用所述酵母生产的酒精饮料和生产所述饮料的方法。

18. 发明申请

US20090311680A1 Method for Identifying Useful Proteins of Brewery Yeast 审中-公开
标题翻译：识别啤酒酵母有用蛋白质的方法
公开(公告)号：US20090311680A1
公开(公告)日：2009-12-17
申请号：US12224371
申请日：2007-02-26
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga , Takeshi Kanamori
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga , Takeshi Kanamori
IPC分类号： C12Q1/68
CPC分类号： C12N15/1034
摘要： The invention relates to a method for identifying a useful protein of brewery yeast. More specifically, the invention relates to (a) cultivating yeast under a predetermined cultivation condition; (b) extracting a protein sample from the cultivation product of the yeast; (c) separating the protein sample by a protein separation means, selecting a target peak or spot, and recovering the target protein or a fragment thereof contained in the peak or spot; (d) determining the amino acid sequence of the target protein; (e) comparing the amino acid sequence determined in step (d) with the amino acid sequence determined in advance based on all or a part of genome sequence information of bottom fermenting yeast; (f) identifying the target protein and the gene encoding the target protein based on the results of comparison; and (g) analyzing functions of the identified gene to identify characters given to the yeast by the gene.
摘要翻译：本发明涉及鉴定啤酒酵母有用蛋白质的方法。更具体地，本发明涉及（a）在预定的培养条件下培养酵母; （b）从酵母的培养产物中提取蛋白质样品; （c）通过蛋白质分离装置分离蛋白质样品，选择目标峰或斑点，并回收峰或点中所含的靶蛋白或其片段; （d）确定靶蛋白的氨基酸序列; （e）基于底部发酵酵母的全部或部分基因组序列信息，比较步骤（d）中确定的氨基酸序列与预先确定的氨基酸序列; （f）基于比较结果鉴定靶蛋白和编码靶蛋白的基因; 和（g）分析所鉴定的基因的功能以鉴定由该基因给予酵母的字符。

19. 发明申请

US20090274794A1 Gene Encoding Cell Wall Mannoprotein and use Thererof 审中-公开
标题翻译：基因编码细胞壁肌肉蛋白并使用其中
公开(公告)号：US20090274794A1
公开(公告)日：2009-11-05
申请号：US11991797
申请日：2006-09-21
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga , Fumihiko Ohmura
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga , Fumihiko Ohmura
IPC分类号： C12N1/19 , C07H21/04 , C07K14/00 , C12N15/63 , C12Q1/68 , C12C11/09 , A23L1/185 , C12G1/073 , C12H1/14
CPC分类号： C12C12/004 , C07K14/395 , C12C12/006 , C12G1/0203 , C12G3/02
摘要： The present invention relates to a brewer's yeast which produces alcoholic beverages having an ability for reducing the haze level, alcoholic beverages produced using such a yeast, and a method of producing such alcoholic beverages. More specifically, the present invention relates to a yeast which can reduce the level of haze in the product by increasing the level of expression of ScCWP2 gene encoding cell wall mannoprotein Cwp2p in brewer's yeast, or non-ScCWP2 gene characteristic to beer yeast, and to a method of producing alcoholic beverages using such a yeast.
摘要翻译：本发明涉及生产具有降低雾度的能力的酒精饮料的啤酒酵母，使用这种酵母生产的含酒精饮料，以及这种酒精饮料的制造方法。更具体地说，本发明涉及通过提高酿酒酵母中编码细胞壁甘露糖蛋白Cwp2p的ScCWP2基因或啤酒酵母特有的非ScCWP2基因的表达水平，能够降低产品的雾度的酵母，使用这样的酵母生产酒精饮料的方法。

20. 发明申请

US20090087515A1 GENE ENCODING ACETOLACTATE SYNTHASE AND USE THEREOF 审中-公开
标题翻译：基因编码乙酰胆碱合成酶及其用途
公开(公告)号：US20090087515A1
公开(公告)日：2009-04-02
申请号：US12279001
申请日：2006-11-30
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12G1/00 , C07K14/395 , C12N15/63 , C12N1/19 , C12C11/00 , C12Q1/68
CPC分类号： C12N9/88
摘要： The present invention relates to an acetolactate synthase gene and use thereof, in particular, a brewery yeast for producing alcoholic beverages with superior flavor, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose capability of producing vicinal diketones, especially diacetyl, that are responsible for off-flavors in products, is reduced by repressing expression level of ILV2 gene encoding an acetolactate synthase (Ilv2p), especially non-ScILV2 gene specific to a lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.
摘要翻译：本发明涉及乙酰乳酸合酶基因及其用途，特别是用于生产具有优良风味的酒精饮料的啤酒酵母，用所述酵母生产的含酒精饮料及其制备方法。更具体地说，本发明涉及通过抑制编码乙酰乳酸合酶（IIv2p）的ILV2基因的表达水平来降低其产生邻位二酮，特别是二乙酰基的产物中异味的能力，特别是非 - -ScILV2基因，特异于啤酒酵母，以及用所述酵母生产酒精饮料的方法。

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