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11. 发明申请

WO2010104593A3 SYSTEMS AND METHODS OF TEMPLATING USING PARTICLES SUCH AS COLLOIDAL PARTICLES 审中-公开
公开(公告)号：WO2010104593A3
公开(公告)日：2010-09-16
申请号：PCT/US2010/000748
申请日：2010-03-12
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , BASF SE , WEITZ, David, A. , KOLTZENBURG, Roland, S. , RIEGER, Jens, B. , STUDART, Andre, R. , WILKING, James, N.
发明人： WEITZ, David, A. , KOLTZENBURG, Roland, S. , RIEGER, Jens, B. , STUDART, Andre, R. , WILKING, James, N.
IPC分类号： B01J2/22 , B01J2/08
摘要： The present invention generally relates to systems and methods for using particle templating, e.g., to produce composites, discrete particles, or the like. In some embodiments, the present invention generally relates to the production of particles using the interstitial spaces between templating elements in a template structure. For example, a plurality of templating elements, which can include colloidal particles, may be arranged to form a template structure. The interstices of the templating elements can provide regions in which a fluid may be introduced. The fluid may be hardened (e.g., solidified) in some cases, e.g., to form a composite comprising the templating elements and the interstitial segments. In certain embodiments, the template structure may then be broken down to release the hardened fluid, e.g., as a plurality of discrete particles.

12. 发明申请

WO2009158024A2 MICROFLUIDIC DROPLETS FOR METABOLIC ENGINEERING AND OTHER APPLICATIONS 审中-公开
标题翻译：用于代谢工程和其他应用的微流控溅射
公开(公告)号：WO2009158024A2
公开(公告)日：2009-12-30
申请号：PCT/US2009/003822
申请日：2009-06-26
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE , WANG, Benjamin, L. , HUMPHRY, Katherine, J. , WEITZ, David, A. , STEPHANOPOULOS, Gregory
发明人： WANG, Benjamin, L. , HUMPHRY, Katherine, J. , WEITZ, David, A. , STEPHANOPOULOS, Gregory
IPC分类号： C12Q1/04 , G01N21/00
CPC分类号： B01L3/502715 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2200/0652 , B01L2200/10 , B01L2300/0681 , B01L2300/0816 , B01L2300/087 , B01L2400/0421
摘要： The present invention relates generally to the use of droplets to culture and/or assay cells or other species. In some cases, the cells or other species may be sorted based upon the results of the culture and/or assay. In some embodiments, cells other species can be encapsulated in droplets and exposed to one or more agents (e.g., a sugar, an indicator dye, etc.). For instance, in some cases, exposure of cells to the agents may result in the production of metabolites or other compounds (e.g., amino acids, proteins, organic acids, etc.) which may be, for example, assayed or otherwise determined. In some embodiments, the reaction of an agent with cells and/or other species within a droplet may reveal a property of the cells or other species (e.g., sugar consumption, growth rate, ability to withstand exposure to the agent, etc.). As an example, cells that produce desired metabolites or exhibit certain properties may be separated from the other cells via sorting techniques. Other aspects of the invention relate to devices or kits for implementing such sorts, methods of promoting such techniques, or the like.
摘要翻译：本发明一般涉及使用液滴来培养和/或测定细胞或其它物种。在一些情况下，可以基于培养和/或测定的结果对细胞或其它物种进行分类。在一些实施方案中，细胞其他物质可以被包封在液滴中并暴露于一种或多种试剂（例如，糖，指示剂染料等）。例如，在一些情况下，细胞暴露于试剂可导致生成代谢物或其它化合物（例如，氨基酸，蛋白质，有机酸等），其可以例如被测定或以其他方式测定。在一些实施方案中，药剂与细胞和/或液滴内的其它物质的反应可以揭示细胞或其他物种的性质（例如，糖消耗，生长速率，耐受试剂暴露的能力等）。作为实例，产生所需代谢物或显示某些特性的细胞可以通过分选技术与其他细胞分离。本发明的其他方面涉及用于实现这种类型的装置或套件，促进这种技术的方法等。

13. 发明申请

WO2008109176A3 ASSAYS AND OTHER REACTIONS INVOLVING DROPLETS 审中-公开
公开(公告)号：WO2008109176A3
公开(公告)日：2008-09-12
申请号：PCT/US2008/003185
申请日：2008-03-07
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , AGRESTI, Jeremy , CHU, Liang-yin , WEITZ, David, A. , KIM, Jin-woong , ROWAT, Amy , SOMMER, Morten , DANTAS, Gautam , CHURCH, George
发明人： AGRESTI, Jeremy , CHU, Liang-yin , WEITZ, David, A. , KIM, Jin-woong , ROWAT, Amy , SOMMER, Morten , DANTAS, Gautam , CHURCH, George
IPC分类号： B01J13/00
摘要： The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.

14. 发明申请

WO2007030501A2 MICROFLUIDIC MANIPULATION OF FLUIDS AND REACTIONS 审中-公开
标题翻译：流体和反应的微流控制
公开(公告)号：WO2007030501A2
公开(公告)日：2007-03-15
申请号：PCT/US2006/034659
申请日：2006-09-07
申请人： BRANDEIS UNIVERSITY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE , FRADEN, Seth , LINK, Darren, Roy , CRISTOBAL-AZKARATE, Galder , JUNG, Uk, Shim , WEITZ, David, A.
发明人： FRADEN, Seth , LINK, Darren, Roy , CRISTOBAL-AZKARATE, Galder , JUNG, Uk, Shim , WEITZ, David, A.
IPC分类号： B41J2/17
CPC分类号： B01L3/502784 , B01L3/06 , B01L3/502792 , B01L2200/0642 , B01L2200/0673 , B01L2200/10 , B01L2200/14 , B01L2200/141 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2400/0487 , Y10T156/1093 , Y10T436/11 , Y10T436/2575
摘要： The present invention relates generally to microfluidic structures, and more specifically, to microfluidic structures and methods including microreactors for manipulating fluids and reactions. In some embodiments, structures and methods for manipulating many (e.g., 1000) fluid samples, i.e., in the form of droplets, are described. Processes such as diffusion, evaporation, dilution, and precipitation can be controlled in each fluid sample. These methods also enable conditions within the fluid samples (e.g., concentration) to be controlled. Manipulation of fluid samples can be useful for a variety of applications, including testing for reaction conditions, e.g., in crystallization, chemical, and biological assays.
摘要翻译：本发明一般涉及微流体结构，更具体地涉及微流体结构和方法，包括用于操纵流体和反应的微反应器。在一些实施例中，描述了用于操纵许多（例如，1000）流体样品，即液滴形式的结构和方法。可以在每个流体样品中控制扩散，蒸发，稀释和沉淀等过程。这些方法还使流体样品中的条件（例如浓度）得以控制。流体样品的操作可用于各种应用，包括测试反应条件，例如在结晶，化学和生物测定中。

15. 发明申请

WO2013074972A1 DEFORMABLE PLATFORMS FOR BIOLOGICAL ASSAYS 审中-公开
标题翻译：生物测定的可变平台
公开(公告)号：WO2013074972A1
公开(公告)日：2013-05-23
申请号：PCT/US2012/065597
申请日：2012-11-16
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , BUTLER, James , KRISHNAN, Ramaswamy , EHRLICHER, Allen , WEITZ, David, A. , FREDBERG, Jeffrey, J. , PARK, Chan, Young
发明人： BUTLER, James , KRISHNAN, Ramaswamy , EHRLICHER, Allen , WEITZ, David, A. , FREDBERG, Jeffrey, J. , PARK, Chan, Young
IPC分类号： C12M1/34
CPC分类号： G01N33/5029 , B05D3/007 , B32B37/24 , B32B2037/243 , C12M23/20 , C12M25/14 , G01N33/4833 , G01N2500/10 , Y10T156/10
摘要： A platform for biological assays includes a base substrate providing structural support to the platform, at least one surface of the base substrate coated with position markers, a first deformable layer positioned on top of the base substrate, and a second deformable layer positioned on top of the first deformable layer, the second deformable layer embedded with deformation markers.
摘要翻译：用于生物测定的平台包括提供对平台的结构支撑的基底基底，涂覆有位置标记的基底基底的至少一个表面，位于基底基底顶部的第一可变形层和位于基底上方的第二可变形层第一可变形层，第二可变形层嵌入变形标记。

16. 发明申请

WO2012109138A1 SYSTEMS AND METHODS FOR SPLITTING DROPLETS 审中-公开
标题翻译：用于分割滴液的系统和方法
公开(公告)号：WO2012109138A1
公开(公告)日：2012-08-16
申请号：PCT/US2012/023961
申请日：2012-02-06
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , ABATE, Adam, R. , WEITZ, David, A.
发明人： ABATE, Adam, R. , WEITZ, David, A.
IPC分类号： B01L3/00
CPC分类号： B01L3/5027 , B01L3/502784 , B01L2200/0673 , B01L2200/12 , B01L2300/0816 , B01L2300/0864 , B01L2300/123 , Y10T137/0318 , Y10T137/85938
摘要： The present invention generally relates to fluidics and microfluidics and, in particular, to creating droplets in a fluidic system. In some aspects, the present invention is generally directed to systems and methods for splitting a parent droplet into two or more droplets, e.g., by urging the parent droplet towards an obstacle to split the parent droplet. In some cases, the parent droplet is split into at least first and second droplets which each are directed to separate channels. In some cases, the channels may be constructed and arranged such that the droplet velocities of the first and second droplets are substantially the same as the velocity of the parent droplet. In some cases, such droplets may be repeatedly split, e.g., a parent droplet is divided into 2 daughter droplets, then each droplet split again, etc., for example, such that one parent droplet may eventually be split into 2 2 , 2 3 , 2 4 , 2 5 , 2 6 , etc. daughter droplets. In some cases, the daughter droplets may be substantially monodisperse.
摘要翻译：本发明一般涉及流体和微流体，特别是涉及在流体系统中产生液滴。在一些方面，本发明一般涉及用于将母液滴分解为两个或更多个液滴的系统和方法，例如通过将母液滴向障碍物推动以分离母液滴。在一些情况下，母液滴被分成至少第一和第二液滴，每个液滴被引导到分离的通道。在一些情况下，可以构造和布置通道，使得第一和第二液滴的液滴速度基本上与母液滴的速度相同。在一些情况下，这样的液滴可以被重复地分开，例如，母液滴被分成2个子液滴，然后每个液滴再次分离等，例如使得一个亲液滴最终可以分成22,23,24 ，25,26等子液滴。在一些情况下，子液滴可以是基本上单分散的。

17. 发明申请

WO2011056546A1 DROPLET CREATION TECHNIQUES 审中-公开
标题翻译： DROPLET创建技术
公开(公告)号：WO2011056546A1
公开(公告)日：2011-05-12
申请号：PCT/US2010/054050
申请日：2010-10-26
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , WEITZ, David, A. , ABATE, Adam, R.
发明人： WEITZ, David, A. , ABATE, Adam, R.
IPC分类号： B01L3/00 , B01F13/00
CPC分类号： B01L3/502761 , B01F3/0807 , B01F13/0062 , B01F13/0071 , B01L3/502784 , B01L2200/0636 , B01L2200/0652 , B01L2300/0681 , Y10T137/0318 , Y10T137/8593
摘要： The present invention is generally related to systems and methods for producing droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, at least one droplet is used to create a plurality of droplets, using techniques such as flow-focusing techniques. In one set of embodiments, a plurality of droplets, containing varying species, can be divided to form a collection of droplets containing the various species therein. A collection of droplets, according to certain embodiments, may contain various subpopulations of droplets that all contain the same species therein. Such a collection of droplets may be used as a library in some cases, or may be used for other purposes.
摘要翻译：本发明通常涉及用于产生液滴的系统和方法。液滴可以包含不同的物种，例如用作文库。在一些情况下，使用诸如流动聚焦技术之类的技术，使用至少一个液滴来产生多个液滴。在一组实施例中，包含不同种类的多个液滴可以被分开以形成其中包含各种物质的液滴的集合。根据某些实施方案，液滴的集合可以包含各种各样的液滴亚组，其中都含有相同的物质。这种液滴的集合在某些情况下可以用作文库，也可以用于其他目的。

18. 发明申请

WO2010151776A2 FLUID INJECTION 审中-公开
标题翻译：流体注射
公开(公告)号：WO2010151776A2
公开(公告)日：2010-12-29
申请号：PCT/US2010/040006
申请日：2010-06-25
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , WEITZ, David, A. , ABATE, Adam, R. , HUNG, Tony , MARY, Pascaline
发明人： WEITZ, David, A. , ABATE, Adam, R. , HUNG, Tony , MARY, Pascaline
IPC分类号： G01N35/10 , B01J19/00 , B01F13/00 , B01F5/06 , B05C9/00
CPC分类号： B01F5/0471 , B01F13/0071 , B01F13/0076 , B01J19/0093 , B01J2219/00837 , B01J2219/00853 , B01J2219/00862 , B01J2219/00889 , B01L3/50273 , B01L3/502784 , B01L2300/0867 , B01L2400/0415 , F16K99/0042 , F16K99/0051 , G01N27/44791 , G01N2035/1034 , Y10T137/0391 , Y10T137/206
摘要： The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field.
摘要翻译：本发明一般涉及用于控制流体的系统和方法，并且在一些情况下涉及将流体流入和/或流出其它流体的系统和方法。作为示例，流体可以被注入到包含在流体通道内的液滴中，或者可以将流体注入到流体通道中以产生液滴。在一些实施例中，可以使用电极将电场施加到一个或多个流体通道，例如至少两个流体通道的相交处。例如，可以通过电场促进第一流体进入和/或流出第二流体。在一些情况下，电场可能破坏第一流体和至少一种其它流体之间的界面。可以通过控制流体和/或流体液滴的各种性质（例如流体液滴的曲率）来控制被推入和/或流出第二流体的第一流体的体积，流速等性质，和/或控制所施加的电场。

19. 发明申请

WO2010033200A2 CREATION OF LIBRARIES OF DROPLETS AND RELATED SPECIES 审中-公开
标题翻译：创作画卷和相关物种的图书馆
公开(公告)号：WO2010033200A2
公开(公告)日：2010-03-25
申请号：PCT/US2009/005184
申请日：2009-09-17
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , WEITZ, David, A. , AGRESTI, Jeremy
发明人： WEITZ, David, A. , AGRESTI, Jeremy
IPC分类号： C40B30/04
CPC分类号： C12Q1/6876 , B01J13/04 , B01J19/0046 , B01J2219/00599 , B01J2219/00709 , B01J2219/00711 , B01J2219/00716 , B01J2219/00722 , C12Q1/6874
摘要： The present invention is generally related to systems and methods for producing a plurality of droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, the fluidic droplets may be rigidified to form rigidified droplets (e.g., gel droplets). In certain embodiments, the droplets may undergo a phase change (e.g., from rigidified droplets to fluidized droplets), as discussed more herein. In some cases, a species may be added internally to a droplet by exposing the droplet to a fluid comprising a plurality of species.
摘要翻译：本发明通常涉及用于生产多个液滴的系统和方法。液滴可以含有不同的物质，例如用作文库。在一些情况下，流体液滴可以被硬化以形成刚性液滴（例如凝胶液滴）。在某些实施方案中，液滴可以经历相变（例如，从刚性液滴到流化液滴），如本文更多地讨论的。在一些情况下，可以通过将液滴暴露于包含多种物质的流体，将物质内部加入到液滴中。

20. 发明申请

WO2009134395A2 MICROFLUIDIC DEVICE FOR STORAGE AND WELL-DEFINED ARRANGEMENT OF DROPLETS 审中-公开
标题翻译：用于储存和定义错误布置的微流体装置
公开(公告)号：WO2009134395A2
公开(公告)日：2009-11-05
申请号：PCT/US2009/002644
申请日：2009-04-28
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , SCHMITZ, Christian , ROWAT, Amy , KOESTER, Sarah , AGRESTI, Jeremy , WEITZ, David, A.
发明人： SCHMITZ, Christian , ROWAT, Amy , KOESTER, Sarah , AGRESTI, Jeremy , WEITZ, David, A.
IPC分类号： G01N35/00 , G01N21/64 , G01N33/50 , C12Q1/02
CPC分类号： G01N21/6452 , B01L3/502784 , B01L7/525 , B01L2200/0621 , B01L2300/0816 , B01L2300/0819 , B01L2300/087 , B01L2300/1827 , B01L2400/0487 , B01L2400/086 , G01N33/5008
摘要： The present invention relates to systems and methods for the arrangement of droplets in pre-determined locations. Many applications require the collection of time- resolved data. Examples include the screening of cells based on their growth characteristics or the observation of enzymatic reactions. The present invention provides a tool and related techniques which addresses this need, and which can be used in many other situations. The invention provides, in one aspect, a tool that allows for stable storage and indexing of individual droplets. The invention can interface not only with microfluidic/microscale equipment, but with macroscopic equipment to allow for the easy injection of liquids and extraction of sample droplets, etc.
摘要翻译：本发明涉及用于在预定位置中布置液滴的系统和方法。许多应用程序需要收集时间分辨数据。实例包括基于其生长特征或观察酶反应来筛选细胞。本发明提供了一种解决该需求的工具和相关技术，并且可以在许多其它情况下使用。本发明在一个方面提供一种允许单个液滴的稳定储存和分度的工具。本发明不仅可以与微流体/微型设备连接，而且可以与宏观设备相接合，以便容易地注入液体和提取样品液滴等。

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