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11. 发明授权

US07482144B2 Method for the production of xylitol 失效
标题翻译：生产木糖醇的方法
公开(公告)号：US07482144B2
公开(公告)日：2009-01-27
申请号：US10341220
申请日：2003-01-13
申请人： Heikki Ojamo , Merja Penttila , Heikki Heikkila , Jaana Uusitalo , Marja Ilmen , Marja-Leena Sarkki , Maija-Leena Vehkomaki
发明人： Heikki Ojamo , Merja Penttila , Heikki Heikkila , Jaana Uusitalo , Marja Ilmen , Marja-Leena Sarkki , Maija-Leena Vehkomaki
IPC分类号： C12P7/18 , C12N7/16 , C12N1/18
CPC分类号： C12P7/18
摘要： The invention relates to a method for the production of xylitol, the method comprising (a1) providing (i) a microorganism having xylanolytic activity, and (ii) a microorganism capable of converting a pentose sugar to xylitol; or (a2) providing a microorganism having xylanolytic activity and being capable of converting a pentose sugar to xylitol, (b) culturing the microorganism of step (a1) (i) or the microorganism of step (a2) in a medium comprising polymer or oligomer materials containing pentose sugars in conditions sufficient for enabling hydrolysis of said polymers or oligomers by the microorganism; (c) producing xylitol in the microorganism of step (a1) (ii) or in the microorganism of step (a2) by bioconversion of the hydrolysis products obtained in step (b), and (d) recovering said xylitol produced. The invention also relates to a microorganism, which has xylanolytic activity and has been genetically modified (i) to enhance its xylanolytic activity, and (ii) to reduce its xylitol metabolism.
摘要翻译：本发明涉及一种生产木糖醇的方法，所述方法包括（a1）提供（i）具有木聚糖分解活性的微生物和（ii）能够将戊糖转化成木糖醇的微生物; 或（a2）提供具有木聚糖分解活性并能够将戊糖转化为木糖醇的微生物，（b）在包含聚合物或低聚物的培养基中培养步骤（a1）（i）的微生物或步骤（a2）的微生物）在足以使微生物水解所述聚合物或低聚物的条件下含有戊糖的材料; （c）通过步骤（b）中获得的水解产物的生物转化和（d）回收所生产的木糖醇，在步骤（a1）（ⅱ）的微生物中或在步骤（a2）的微生物中生产木糖醇。本发明还涉及一种具有木聚糖分解活性且经遗传修饰的微生物（i）增强其木聚糖分解活性，和（ii）降低其木糖醇代谢。

12. 发明授权

US5939294A Immobilization of microogranisms on weakly basic anion exchange substance for producing isomaltulose 失效
标题翻译：将微生物固定在用于转化蔗糖的阴离子交换物质上
公开(公告)号：US5939294A
公开(公告)日：1999-08-17
申请号：US857808
申请日：1997-05-16
申请人： Marja-Leena Sarkki , Heikki Heikkila , Tapio Viljava
发明人： Marja-Leena Sarkki , Heikki Heikkila , Tapio Viljava
IPC分类号： C12N9/90 , C12N11/12 , C12P19/24 , C12P19/12 , C12N11/02 , C12N11/08
CPC分类号： C12R1/01 , C12N11/12 , C12N9/90 , C12P19/24
摘要： Isomaltulose-forming microorganisms are immobilized on a carrier that is a weakly basic anion exchange substance in the form of a substantially non-compressible porous particulate solid material, and are used for isomerization of sucrose to isomaltulose. A preferred carrier contains microfibers or microparticles of diethylaminoethyl cellulose adherently bound by agglomeration with polystyrene. The isomerization may be a continuous conversion in one or more columns packed with the carrier. Isomaltulose may be hydrogenated to form isomalt for use in sweetening. Microorganisms can be immobilized on the carrier by feeding microorganisms to a column containing the carrier. After microorganism immobilization, the carrier may be treated with a crosslinking and/or flocculating compound. Regeneration of the carrier is carried out by removing microorganisms, washing and reloading with fresh microorganisms.
摘要翻译：异麦芽酮糖形成微生物固定在作为基本上不可压缩的多孔颗粒固体材料形式的弱碱性阴离子交换物质的载体上，并用于将蔗糖异构化成异麦芽酮糖。优选的载体包含通过与聚苯乙烯附聚粘附结合的二乙基氨基乙基纤维素的微纤维或微粒。异构化可以是在一个或多个填充有载体的柱中的连续转化。异麦芽酮糖可以被氢化以形成用于增甜的异麦芽酮糖醇。微生物可以通过将微生物送入含有载体的柱中而固定在载体上。微生物固定后，载体可以用交联和/或絮凝化合物处理。通过除去微生物，用新鲜的微生物清洗和重新装载来进行载体的再生。

13. 发明授权

US06512110B1 Process for the production of xylose from a paper-grade hardwood pulp 失效
标题翻译：用于从纸级硬木纸浆生产木糖的方法
公开(公告)号：US06512110B1
公开(公告)日：2003-01-28
申请号：US09456902
申请日：1999-12-08
申请人： Heikki Heikkila , Marja-Leena Sarkki , Vili Ravanko , Mirja Lindroos
发明人： Heikki Heikkila , Marja-Leena Sarkki , Vili Ravanko , Mirja Lindroos
IPC分类号： C07H106
CPC分类号： C12P19/14 , C12N9/248 , C13B20/02 , C13K13/002 , D21C5/005 , D21C9/00 , D21C9/002
摘要： The invention relates to a process for the production of xylose from a paper-grade hardwood pulp comprising the steps of extracting xylan contained in said pulp into a liquid phase, subjecting the xylan contained in the obtained liquid phase to conditions sufficient to hydrolyze xylan to xylose, and recovering the xylose, wherein the extracting step includes at least one treatment of an aqueous suspension of said pulp or an alkali-soluble material thereof with a xylanase enzyme.
摘要翻译：本发明涉及从纸级硬木浆生产木糖的方法，包括以下步骤：将所述纸浆中含有的木聚糖提取到液相中，使得到的液相中含有的木聚糖使足够水解木聚糖至木糖的条件并回收木糖，其中提取步骤包括至少一种处理所述纸浆的水性悬浮液或其碱溶性物质与木聚糖酶。

14. 发明授权

US5932452A Process for the production of xylose by hydrolysis of hemicellulose by immobilized enzymes 失效
标题翻译：通过固定化酶水解半纤维素来生产木糖的方法
公开(公告)号：US5932452A
公开(公告)日：1999-08-03
申请号：US289803
申请日：1994-08-12
申请人： Annikka Mustranta , Kaisa Poutanen , Heikki Heikkila , Marja-Leena Sarkki
发明人： Annikka Mustranta , Kaisa Poutanen , Heikki Heikkila , Marja-Leena Sarkki
IPC分类号： C12N11/08 , C12N9/24 , C12N11/02 , C12P19/02 , C12P19/14
CPC分类号： C12Y302/01032 , C12N11/02 , C12N9/248 , C12P19/02 , C12Y302/01008 , C12Y302/01037 , C12Y302/01072
摘要： The invention relates to a process for the hydrolysis of a hemicellulose substrate containing xylo-oligomers with an immobilized enzyme by adjusting the pH of an aqueous solution containing hemicellulose oligosaccharides and optionally ionic components to a value within a weakly acidic range, and contacting the solution with a hemicellulolytic enzyme or mixture of enzymes immobilized on a regenerable weak cation exchange carrier without fixing additives, and recovering xylose from the solution. The invention also concerns an enzyme preparation to be used in the process, comprising a weak cation exchange carrier and a hemicellulolytic enzyme or mixture of enzymes immobilized on said carrier.
摘要翻译： PCT No.PCT / FI90 / 00206 Sec。 371日期：1992年3月27日 102（e）日期1992年3月27日PCT提交1990年9月21日PCT公布。出版物WO91 / 03566 日期1991年3月21日本发明涉及通过将含有半纤维素寡糖和任选的离子组分的水溶液的pH调节至弱酸性范围内的值来水解具有固定化酶的含有低聚木糖的半纤维素底物的方法，并将溶液与固定在可再生的弱阳离子交换载体上的半纤维素分解酶或酶的混合物接触而不固定添加剂，并从溶液中回收木糖。本发明还涉及在该方法中使用的酶制剂，其包含弱阳离子交换载体和半纤维素分解酶或固定在所述载体上的酶的混合物。

15. 发明授权

US4448790A Process for fractioning grain flour into components of food quality 失效
$Process for fractioning grain flour into components of food quality$
标题翻译：将谷物粉碎成食品质量成分的方法
公开(公告)号：US4448790A
公开(公告)日：1984-05-15
申请号：US340646
申请日：1982-01-19
申请人： Marja-Leena Sarkki , Martti Soderstrom , Hannu Maunula , Annika Korhonen
发明人： Marja-Leena Sarkki , Martti Soderstrom , Hannu Maunula , Annika Korhonen
IPC分类号： A23J1/12 , C08B30/02 , C12P19/14 , C12P19/18 , C12P19/20 , A23L1/09 , A23L1/195
CPC分类号： C12P19/20 , A23J1/12
摘要： The present invention is concerned with a process by means of which the fraction of grain flour that remains after separation of the main part of the starch and of the gluten-type protein, when the grain contains gluten, is divided into two products of food quality. One of these products consists mainly of the albumin-globulin-type, water soluble proteins and the other one, a syrupy product, consists mainly of sugars obtained as products of hydrolysis of small-particle starch of grain. The homogenous slurry obtained after separation of ordinary grain starch and gluten from the grain flour by known methods is heated at a temperature of at least 120.degree. C., treated at a temperature of at the most 90.degree. C. simultaneously by means of alpha-amylase and beta-glucanase, the produced protein precipitate is separated from the sludge, washed, and dried. The remaining clear fraction is sugared by means of amyloglucosidase of fungalamylase.
摘要翻译：本发明涉及一种方法，其中当谷物含有谷蛋白时，淀粉主要部分和谷蛋白型蛋白质分离后残留的谷物粉末的分数分为食品质量的两个产品。这些产品中的一种主要由白蛋白 - 球蛋白型水溶性蛋白质组成，另一种是糖浆状产品，主要由作为谷粒小粒淀粉水解产物获得的糖组成。通过已知方法从谷物粉中分离出普通谷物淀粉和谷蛋白后获得的均匀浆料在至少120℃的温度下加热，同时在最高90℃的温度下，通过α- 淀粉酶和β-葡聚糖酶，将所产生的蛋白质沉淀物与污泥分离，洗涤并干燥。剩余的透明级分通过真菌淀粉酶的淀粉葡糖苷酶进行糖化。

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