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11. 发明专利

JP2009171960A Biological system for producing polyhydroxyalkanoate polymer containing 4-hydroxyacid 审中-公开
标题翻译：生产含有羟基羟基烷基酯的聚羟基烷基酯聚合物的生物体系
公开(公告)号：JP2009171960A
公开(公告)日：2009-08-06
申请号：JP2008328227
申请日：2008-12-24
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： HUISMAN GJALT W , SKRALY FRANK A , MARTIN DAVID P , PEOPLES OLIVER P
IPC分类号： C12N1/21 , C12N15/09 , C08G63/06 , C12N9/02 , C12N9/10 , C12N15/53 , C12N15/54 , C12N15/82 , C12P7/52 , C12P7/62 , C12R1/19
CPC分类号： C12N9/0008 , C08G63/06 , C12N9/1029 , C12N9/1096 , C12N15/8243 , C12P7/625
摘要： PROBLEM TO BE SOLVED: To provide a recombinant process capable of incorporating a further gene into a PHB(polyhydroxybutyric acid) producer in order to create a new strain synthesizing a monomer of an alternative polyhydroxyalkanoate(PHA) (e.g. 4-hydroxybutyric acid/4HB), and to provide a method for stably creating an organism synthesizing a 4HB-containing PHA as either of sole construct or comonomer. SOLUTION: The method uses such a recombinant host that its genome is stably incorporated with a gene encoding a heterologous enzyme selected from the group consisting of a PHA synthase and a 4HB-CoA transferase. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：为了提供能够将另外的基因并入PHB（多羟基丁酸）生产者中的重组方法，以便产生合成替代的聚羟基链烷酸酯（PHA）的单体的新菌株（例如4-羟基丁酸 / 4HB），并且提供用于稳定地产生合成含有4HB的PHA作为唯一构建体或共聚单体的生物体的方法。解决方案：该方法使用这样的重组宿主，其基因组稳定掺入编码选自PHA合酶和4HB-CoA转移酶的异源酶的基因。版权所有（C）2009，JPO＆INPIT

12. 发明专利

JP2009112315A Method for isolating polyhydroxyalkanoate from plant 审中-公开
标题翻译：从植物中分离聚羟基烷基酯的方法
公开(公告)号：JP2009112315A
公开(公告)日：2009-05-28
申请号：JP2009045960
申请日：2009-02-27
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： MARTIN DAVID P , PEOPLES OLIVER P , WILLIAMS SIMON F
IPC分类号： A01H1/00 , C12P7/62 , C08G63/89 , C08H99/00 , C12N5/10 , C12N15/09
CPC分类号： C08G63/89 , C12P7/625
摘要： PROBLEM TO BE SOLVED: To provide methods for separating polyhydroxyalkanoates ("PHA") from plants, such as transgenic oil crop plants.
SOLUTION: To isolate the PHA, in one embodiment, a biomass derived from an oil crop plant is pre-processed, for example by grinding, crushing or rolling. Then the oil is extracted from the biomass with a first solvent (in which solvent the oil is soluble and the PHA are not highly soluble) to remove the oil. Then the biomass can be extracted with a second solvent (in which solvent the PHA is soluble) to separate the PHA from the biomass. Alternatively, the PHA-containing biomass is treated with a chemical or biochemical agent to chemically transform the PHA into a PHA derivative. Then the PHA derivative is separated from the mixture by using, for example, a physical separation process.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供从植物如转基因油料作物植物中分离聚羟基链烷酸酯（“PHA”）的方法。解决方案：为了分离PHA，在一个实施方案中，来自油料作物植物的生物质被预处理，例如通过研磨，破碎或轧制。然后用第一溶剂（其中油溶于溶剂，PHA不高度溶解）从生物质中提取油，以除去油。然后生物质可以用第二溶剂（其中PHA溶于其中的溶剂）提取，以将PHA与生物质分离。或者，用化学或生化试剂处理含PHA的生物质，以将PHA化学转化为PHA衍生物。然后通过使用例如物理分离方法将PHA衍生物与混合物分离。版权所有（C）2009，JPO＆INPIT

13. 发明专利

JP2009082147A Biological system for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxy acid 审中-公开
标题翻译：含有4-羟基酸的聚羟基烷酸酯聚合物的生物体系
公开(公告)号：JP2009082147A
公开(公告)日：2009-04-23
申请号：JP2008328226
申请日：2008-12-24
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： HUISMAN GJALT W , SKRALY FRANK A , MARTIN DAVID P , PEOPLES OLIVER P
IPC分类号： C12N1/21 , C12N15/09 , C08G63/06 , C12N9/02 , C12N9/10 , C12N15/53 , C12N15/54 , C12N15/82 , C12P7/52 , C12P7/62 , C12R1/19
CPC分类号： C12N9/0008 , C08G63/06 , C12N9/1029 , C12N9/1096 , C12N15/8243 , C12P7/625
摘要： PROBLEM TO BE SOLVED: To provide the technology and process for stably making a transgenic organism synthesizing PHA including 4-hydroxy-butyric acid as only one component or as co-monomer by using the recombination process in which another gene is introduced into the transgenic PHP producer, in order to create a new strain synthesizing the substitute PHA monomer (for example, 4-HB). SOLUTION: A recombinant host that stably incorporates the gene encoding different kinds of enzyme selected from polyhydroxyalkanoate synthase and 4HB-CoA transferase into the genome. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供用于稳定制备转基因生物的技术和方法，其通过使用引入另一种基因的重组方法将包含4-羟基 - 丁酸的PHA作为仅一种成分或作为共聚单体合成转基因PHP生产者，以产生合成替代PHA单体（例如4-HB）的新菌株。解决方案：将编码不同种类的选自聚羟基链烷酸合酶和4HB-CoA转移酶的基因的基因稳定并入基因组的重组宿主。版权所有（C）2009，JPO＆INPIT

14. 发明专利

JP2008248258A Method for isolating polyhydroxyalkanoate from plant 有权
标题翻译：从植物中分离聚羟基烷基酯的方法
公开(公告)号：JP2008248258A
公开(公告)日：2008-10-16
申请号：JP2008180815
申请日：2008-07-10
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： MARTIN DAVID P , PEOPLES OLIVER P , WILLIAMS SIMON F
IPC分类号： A01H1/00 , C08G63/89 , C08H99/00 , C12N5/10 , C12N15/09 , C12P7/62
CPC分类号： C08G63/89 , C12P7/625
摘要： PROBLEM TO BE SOLVED: To provide a method for separating polyhydroxyalkanoates ("PHAs") from plants such as a transgenic oil crop plant.
SOLUTION: In an embodiment of the method, to isolate the PHAs, a biomass derived from an oil crop plant is preprocessed by, for example, grinding, crushing or rolling. Oil is then extracted from the biomass by using a first solvent (in which the oil is soluble and the PHAs are not highly soluble) to remove the oil. Then, the biomass can be extracted by using a second solvent (in which the PHAs are soluble) to separate the PHAs from the biomass. Alternatively, the PHA-containing biomass is treated with a chemical or biochemical agent to chemically transform the PHA into a PHA derivative. The PHA derivative is then separated from the mixture by using a physical separation process.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供从植物如转基因油料作物植物中分离聚羟基链烷酸酯（“PHAs”）的方法。解决方案：在该方法的一个实施方案中，为了分离PHAs，通过例如研磨，破碎或轧制来预处理来自油料作物的生物质。然后通过使用第一溶剂（其中油可溶而PHAs不是高度可溶的））从生物质中提取油以除去油。然后，可以通过使用第二溶剂（其中可溶于PHAs）来提取生物质，以将PHAs与生物质分离。或者，用化学或生化试剂处理含PHA的生物质，以将PHA化学转化为PHA衍生物。然后通过使用物理分离方法将PHA衍生物与混合物分离。版权所有（C）2009，JPO＆INPIT

15. 发明专利

JP2010279358A Transgenic microbial polyhydroxy alkanoate producer 审中-公开
标题翻译：转基因微生物聚羟基烷基酯生产商
公开(公告)号：JP2010279358A
公开(公告)日：2010-12-16
申请号：JP2010137239
申请日：2010-06-16
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： HUISMAN GJALT W , PEOPLES OLIVER P , SKRALY FRANK
IPC分类号： C12N1/21 , C12N15/09 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/88 , C12N15/90 , C12P7/62 , C12Q1/02 , C12Q1/68 , C12R1/19
CPC分类号： C12N9/1029 , C12N9/00 , C12N9/0004 , C12N9/0006 , C12N9/1025 , C12N9/13 , C12N9/88 , C12N15/90 , C12P7/625 , Y10S435/829 , Y10S435/831 , Y10S435/877
摘要： PROBLEM TO BE SOLVED: To provide transgenic microbial strains which contain genes required for poly(3-hydroxyalkanoate) (PHA) formation integrated on the chromosome. SOLUTION: At least one gene participating in synthesis of PHA selected from the group consisting of thiolase, reductase, PHB synthase, PHA synthase, acyl-CoA transferase and enoyl-CoA hydratase is integrated using standard techniques, preferably transposon mutagenesis. In a preferred embodiment wherein multiple genes are incorporated, the gene is incorporated as an operon. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供含有整合在染色体上的聚（3-羟基链烷酸酯）（PHA）形成所需基因的转基因微生物菌株。解决方案：使用标准技术（优选转座子诱变）整合至少一种参与合成PHA的基因，所述基因选自硫醇酶，还原酶，PHB合酶，PHA合成酶，酰基辅酶A转移酶和烯酰辅酶A水合酶。在其中掺入多个基因的优选实施方案中，该基因作为操纵子并入。版权所有（C）2011，JPO＆INPIT

16. 发明专利

JP2009291204A Modification of fatty acid metabolism in plant 审中-公开
标题翻译：植物中脂肪酸代谢的修改
公开(公告)号：JP2009291204A
公开(公告)日：2009-12-17
申请号：JP2009181255
申请日：2009-08-04
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： PEOPLES OLIVER P , BOYNTON LAURA , HUISMAN GJALT W , MOLONEY MAURICE , PATTERSON NII , SNELL KRISTI
IPC分类号： A01H5/00 , C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N15/52 , C12N15/82 , C12P7/62
CPC分类号： C12N15/8289 , C12N15/52 , C12N15/8243 , C12N15/8247
摘要： PROBLEM TO BE SOLVED: To provide a method and a system for modifying fatty acid biosynthesis and oxidation in plants in order to make new polymers. SOLUTION: Two enzymes are essential: a hydratase such as D-specific enoyl-CoA hydratase, for example, the hydratase obtained from Aeromonas caviae, and a β-oxidase system. Some plants have such a β-oxidase system enough to modify polymer synthesis when the plants are engineered to express the hydratase. Examples demonstrate production of polymer by expression of these enzymes in transgenic plants. Examples also demonstrate that modifications in fatty acid biosynthesis can be used to alter plant phenotypes, thereby decreasing or eliminating seed production and increasing green plant biomass, as well as producing polyhydroxyalkanoates. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供用于修饰植物中脂肪酸生物合成和氧化的方法和系统，以制备新的聚合物。解决方案：两种酶是必不可少的：水合酶如D-特异性烯酰辅酶A水合酶，例如从气单胞菌（Aeromonas caviae）获得的水合酶和β-氧化酶系统。一些植物具有这样的β-氧化酶体系，足以在植物被工程化以表达水合酶时修饰聚合物合成。实施例证明通过在转基因植物中表达这些酶来生产聚合物。实例还表明，脂肪酸生物合成的改变可用于改变植物表型，从而减少或消除种子生产和增加绿色植物生物量，以及生产聚羟基链烷酸酯。版权所有（C）2010，JPO＆INPIT

17. 发明专利

JP2007277574A Method for isolating polyhydroxyalkanoate from plant 审中-公开
标题翻译：从植物中分离聚羟基烷基酯的方法
公开(公告)号：JP2007277574A
公开(公告)日：2007-10-25
申请号：JP2007151037
申请日：2007-06-06
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： MARTIN DAVID P , PEOPLES OLIVER P , WILLIAMS SIMON F
IPC分类号： A01H1/00 , C08G63/06 , B01D11/02 , C08G63/89 , C08H99/00 , C08L101/16 , C12N5/10 , C12N15/09 , C12P7/62
CPC分类号： C08G63/89 , C12P7/625
摘要： PROBLEM TO BE SOLVED: To provide a method for separating polyhydroxyalkanoate ("PHA") from a plant such as transgenic oil crop plant.
SOLUTION: In order to isolate PHA, in an embodiment, a biomass derived from an oil crop plant is pre-processed, for example, by grinding, crushing or rolling. Then, the oil is extracted from the biomass with the first solvent in which the oil is soluble and the PHA is not highly soluble and the oil is removed. The biomass then can be extracted with the second solvent in which the PHA is soluble, to separate the PHA from the biomass. Alternatively, the PHA-containing biomass is treated with a chemical or biochemical agent, such as an enzyme, to chemically transform the PHA into a PHA derivative. The PHA derivative then is separated from the mixture using, for example, a physical separation process such as distillation, extraction or chromatography.
COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：提供从植物如转基因油料作物植物中分离聚羟基链烷酸酯（“PHA”）的方法。解决方案：为了分离PHA，在一个实施方案中，来自油料作物植物的生物质经预处理，例如通过研磨，破碎或轧制。然后，用可溶于油的第一溶剂和PHA不易溶解并除去油的生物质中提取油。然后可以用可溶于PHA的第二溶剂萃取生物质，以将PHA与生物质分离。或者，将含PHA的生物质用化学或生化剂如酶处理，以将PHA化学转化为PHA衍生物。然后使用例如物理分离方法如蒸馏，萃取或色谱法将PHA衍生物与混合物分离。版权所有（C）2008，JPO＆INPIT

18. 发明专利

JP2003310262A Biological system for manufacture of polyhydroxyalkanoate polymer containing 4-hydroxyacid 审中-公开
标题翻译：生产含有4-羟基酸的聚羟基烷基酯聚合物的生物体系
公开(公告)号：JP2003310262A
公开(公告)日：2003-11-05
申请号：JP2003132325
申请日：2003-05-09
申请人： Metabolix Inc , メタボリックス,インコーポレイテッド
发明人： HUISMAN GJALT W , SKRALY FRANK A , MARTIN DAVID P , PEOPLES OLIVER P
IPC分类号： C12N15/09 , C08G63/06 , C12N1/21 , C12N9/02 , C12N9/10 , C12N15/53 , C12N15/54 , C12N15/82 , C12P7/52 , C12P7/62 , C12R1/19
CPC分类号： C12N9/0008 , C08G63/06 , C12N9/1029 , C12N9/1096 , C12N15/8243 , C12P7/625
摘要： PROBLEM TO BE SOLVED: To provide a recombinant process to transfer a further gene into a transgenic PHB producer to produce a new strain synthesizing a monomer of a substitute PHA (poly [(R)-3-hydroxyalkanoate]) (e.g. 4HB (copolymer poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV)) and provide a technique and procedure to stably produce a transgenic organism synthesizing a PHA containing 4-hydroxybutyric acid as the sole constituent or a comonomer. SOLUTION: A gene encoding a heterologous enzyme selected from polyhydroxyalkanoate synthase and 4HB-CoA transferase is stably transferred into a genom to obtain a recombinant host. COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：提供重组方法将另外的基因转移到转基因PHB生产者中以产生合成替代PHA（聚[（R）-3-羟基链烷酸酯]）单体的新菌株（例如4HB （共聚物（3-羟基丁酸 - 共-3-羟基戊酸）（PHBV）），提供稳定地生产合成含有4-羟基丁酸作为唯一成分或共聚单体的PHA的转基因生物的技术和方法。 >解决方案：将编码选自聚羟基链烷酸合酶和4HB-CoA转移酶的异源酶的基因稳定转移到基因组中以获得重组宿主。（C）2004，JPO

19. 发明申请

WO9961624A2 POLYHYDROXYALKANOATE BIOPOLYMER COMPOSITIONS 审中-公开
标题翻译：聚羟基烷基酯生物聚合物组合物
公开(公告)号：WO9961624A2
公开(公告)日：1999-12-02
申请号：PCT/US9911417
申请日：1999-05-21
申请人： METABOLIX INC
发明人： SKRALY FRANK A , PEOPLES OLIVER P
IPC分类号： C12N15/09 , C08G63/06 , C12N1/21 , C12N9/00 , C12N9/10 , C12P7/62 , C12R1/19 , C12N15/52 , C12N15/54 , C12N15/70
CPC分类号： C12N9/93 , C08G63/06 , C12N9/1029 , C12P7/625
摘要： Several novel PHA polymer compositions produced using biological sytems include monomers such as 3-hydroxybutyrate, 3-hydroxypropionate, 2-hydroxybutyrate, 3-hydroxyvalerate, 4-hydroxybutyrate, 4-hydroxyvalerate and 5-hydroxyvalerate. These PHA compositions can readily be extended to incorporate additional monomers including, for example, 3-hydroxyhexanoate, 4-hydroxyhexanoate, 6-hydroxyhexanoate or other longer chain 3-hydroxyacids containing seven or more carbons. This can be accomplished by taking natural PHA producers and mutating through chemical or transposon mutagenesis to delete or inactivate genes encoding undesirable activities. Alternatively, the strains can be genetically engineered to express only those enzymes required for the production of the desired polymer composition. Methods for genetically engineering PHA producing microbes are widely known in the art (Huisman and Madison, 1998, Microbiology and Molecular Biology Reviews, 63 : 21-53). These polymers have a variety of uses in medical, industrial and other commercial areas.
摘要翻译：使用生物系统生产的几种新型PHA聚合物组合物包括单体如3-羟基丁酸酯，3-羟基丙酸酯，2-羟基丁酸酯，3-羟基戊酸酯，4-羟基丁酸酯，4-羟基戊酸酯和5-羟基戊酸酯。这些PHA组合物可以容易地延伸以加入另外的单体，包括例如3-羟基己酸酯，4-羟基己酸酯，6-羟基己酸酯或其它含有7个或更多个碳的长链3-羟基酸。这可以通过使用天然PHA生产者和通过化学或转座子诱变进行突变来删除或灭活编码不期望的活性的基因来实现。或者，菌株可以被遗传工程化以仅表达生产所需聚合物组合物所需的那些酶。用于遗传工程化生产PHA的微生物的方法在本领域中是众所周知的（Huisman and Madison，1998，Microbiology and Molecular Biology Reviews， 63：21-53）。这些聚合物在医疗，工业和其他商业领域具有多种用途。

20. 发明申请

WO0240690A2 PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM FATTY ACID BIOSYNTHETIC PATHWAYS 审中-公开
标题翻译：从脂肪酸生产途径生产中链长度聚羟基烷酸
公开(公告)号：WO0240690A2
公开(公告)日：2002-05-23
申请号：PCT/US0143686
申请日：2001-11-16
申请人： METABOLIX INC
发明人： AQUIN STEPHANIE , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： A01H5/00 , C12N1/21 , C12N9/00 , C12N9/10 , C12N9/16 , C12N15/09 , C12N15/54 , C12N15/55 , C12N15/82 , C12P7/42 , C12Q1/02 , C12Q1/25 , C12Q1/44 , C12Q1/48
CPC分类号： C12N9/93 , C12N9/00 , C12N9/10 , C12N9/1029 , C12N9/16 , C12N15/8243
摘要： Methods for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, have been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase with substrate specificity for medium chain length 3-hydroxy fatty acids, and a medium chain length PHA synthase, has been developed. Acyl CoA synthetase activity can be supplied either by the endogenous acyl CoA synthetase of the host organism, when sufficiently expressed, or the host organism's activity can be supplemented by the expression of a recombinant acyl CoA synthetase gene. New strategies are described for plant based PHA production in the chloroplasts, cytosol, and peroxisomes of biomass crops as well as the plastids, cytosol, and peroxisomes of oil seed crops.
摘要翻译：已经开发了使用3-羟基酰基ACP硫酯酶，PHA合成酶和酰基辅酶A合成酶从脂肪酸生物合成途径生产聚羟基链烷酸酯（PHA）的方法。使用具有3-羟基酰基ACP硫酯酶的催化活性的酶，非天然细菌PHA生产者和植物中的脂肪酸生物合成途径的PHA生产的方法，具有对于中链长度3-羟基脂肪酸具有底物特异性的酰基辅酶A合成酶，和中链长度PHA合酶。酰基辅酶A合成酶活性可以通过宿主生物体的内源酰基辅酶A合成酶提供，当被充分表达时，或者宿主生物的活性可以通过重组酰基辅酶A合成酶基因的表达补充。描述了用于生物量作物的叶绿体，细胞溶质和过氧化物酶体以及油料种子作物的质体，细胞溶质和过氧化物酶体的基于植物的PHA生产的新策略。

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