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    • 15. 发明申请
    • ANALYTE SENSING VIA ACRIDINE-BASED BORONATE BIOSENSORS
    • 通过基于ACRIDINE的BORONATE生物传感器进行分析
    • US20050191761A1
    • 2005-09-01
    • US09952563
    • 2001-09-12
    • Aaron HeissJoseph WalshDavid VachonGlenn NoronhaJonathan ReillyBill PonderWilliam Van Antwerp
    • Aaron HeissJoseph WalshDavid VachonGlenn NoronhaJonathan ReillyBill PonderWilliam Van Antwerp
    • C12Q1/54G01N33/66G01N33/543
    • G01N33/66C12Q1/54Y10T436/144444
    • Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. These biosensor molecules address the problem of obtaining fluorescence emission at wavelengths greater than about 500 nm. Biosensor molecules generally include an (1) an acridine-based fluorophore, (2) a linker moiety and (3) a boronate substrate recognition/binding moiety, which binds polyhydroxylate analytes, such as glucose. These biosensor molecules further include a “switch” element that is drawn from the electronic interactions among these submolecular components. This fluorescent switch is generally “off” in the absence of bound polyhydroxylate analyte and is generally “on” in the presence of bound polyhydroxylate analyte. Thus, the reversible binding of a polyhydroxylate analyte essentially turns the fluorescent switch “on” and “off”. This property of the biosensor molecules, as well as their ability to emit fluorescent light at greater than about 500 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.
    • 描述荧光生物传感器分子,荧光生物传感器和系统,以及制造和使用这些生物传感器分子和系统的方法。 这些生物传感器分子解决了在大于约500nm的波长处获得荧光发射的问题。 生物传感器分子通常包括(1)基于吖啶的荧光团,(2)接头部分和(3)结合多羟基化合物分析物如葡萄糖的硼酸酯底物识别/结合部分。 这些生物传感器分子还包括从这些亚分子组分之间的电子相互作用中抽出的“开关”元件。 在不存在结合的多羟基甲酸盐分析物的情况下,该荧光开关通常是“关闭”的,并且在结合的多羟基化合物分析物的存在下通常是“开”的。 因此,多羟基化合物分析物的可逆结合基本上使得荧光开关“开”和“关”。 生物传感器分子的这种性质以及它们在大于约500nm处发射荧光的能力使得这些生物传感器分子特别适用于检测和测量体内葡萄糖浓度。