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11. 发明申请

US20070224602A1 Thiocarbonate linkers for polynucleotides 有权
标题翻译：多核苷酸的硫代碳酸酯接头
公开(公告)号：US20070224602A1
公开(公告)日：2007-09-27
申请号：US11389326
申请日：2006-03-23
申请人： Douglas J. Dellinger , Zoltan Timar , Joel Myerson , Geraldine Dellinger , Marvin Caruthers
发明人： Douglas J. Dellinger , Zoltan Timar , Joel Myerson , Geraldine Dellinger , Marvin Caruthers
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C07H21/04
摘要： In various embodiments of the invention, novel compositions having a polynucleotide bound to a substrate via a cleavable linker are provided, and methods of cleaving a polynucleotide from a substrate are provided.
摘要翻译：在本发明的各种实施方案中，提供了具有通过可切割连接子与底物结合的多核苷酸的新型组合物，并提供了从底物切割多核苷酸的方法。

12. 发明授权

US07135565B2 Synthesis of polynucleotides using combined oxidation/deprotection chemistry 有权
公开(公告)号：US07135565B2
公开(公告)日：2006-11-14
申请号：US09916369
申请日：2001-07-27
申请人： Douglas J. Dellinger , Michael G. M. Perbost , Marvin H. Caruthers , Jason R. Betley
发明人： Douglas J. Dellinger , Michael G. M. Perbost , Marvin H. Caruthers , Jason R. Betley
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04 , A01N43/04 , A61K31/70
CPC分类号： C07H21/00 , Y02P20/55
摘要： A method of synthesizing a polynucleotide which can, for example, be used during fabrication of an array. A second nucleoside is coupled to a first nucleoside through a phosphite linkage, with the second nucleoside having a hydroxyl protecting group that is a non-carbonate protecting group. The product of the foregoing step is exposed to a composition which both oxidizes the formed phosphite to a phosphate and deprotects the protected hydroxyl of the coupled nucleoside. The method has particular application to fabricating an addressable array of polynucleotides on a substrate which carries substrate bound moieties each with a hydroxyl group.

13. 发明授权

US06319674B1 Methods for attaching substances to surfaces 失效
标题翻译：将物质附着于表面的方法
公开(公告)号：US06319674B1
公开(公告)日：2001-11-20
申请号：US09397527
申请日：1999-09-16
申请人： Geraldine Fulcrand , Douglas J. Dellinger , Steven M. Lefkowitz
发明人： Geraldine Fulcrand , Douglas J. Dellinger , Steven M. Lefkowitz
IPC分类号： G01N3353
CPC分类号： G01N33/54353
摘要： Methods are disclosed for immobilizing a substance to a surface. A surface is employed that comprises a linking group consisting of a first portion comprising a hydrocarbon chain, optionally substituted, and a second portion comprising an alkylene oxide or an alkylene imine wherein the alkylene is optionally substituted. One end of the first portion is attached to the surface and one end of the second portion is attached to the other end of the first portion chain by means of an amine or an oxy functionality. The second portion terminates in an amine or a hydroxy functionality. The surface is reacted with the substance to be immobilized under conditions for attachment of the substance to the surface by means of the linking group. Compositions of matter and reaction systems are also disclosed.
摘要翻译：公开了将物质固定在表面上的方法。使用表面，其包含由包含任选取代的烃链的第一部分和包含烯化氧或亚烷基亚胺的第二部分组成的连接基团，其中所述亚烷基是任选取代的。第一部分的一端附接到表面，并且第二部分的一端通过胺或氧官能团连接到第一部分链的另一端。第二部分终止于胺或羟基官能团。在通过连接基团将物质附着到表面的条件下，表面与要固定的物质反应。还公开了物质和反应体系的组成。

14. 发明授权

US06258454B1 Functionalization of substrate surfaces with silane mixtures 失效
公开(公告)号：US06258454B1
公开(公告)日：2001-07-10
申请号：US09145015
申请日：1998-09-01
申请人： Steven M. Lefkowitz , Geraldine Fulcrand , Douglas J. Dellinger , Charles Z. Hotz
发明人： Steven M. Lefkowitz , Geraldine Fulcrand , Douglas J. Dellinger , Charles Z. Hotz
IPC分类号： B32B1706
CPC分类号： B82Y30/00 , B05D1/185 , B32B17/10688 , B82Y40/00 , G01N33/54353 , Y10T428/261 , Y10T428/31612 , Y10T428/31663 , Y10T428/31667
摘要： Low surface energy functionalized surfaces on solid supports are provided by treating a solid support having hydrophilic moieties on its surface with a derivatizing composition containing a mixture of silanes. A first silane provides the desired reduction in surface energy, while the second silane enables functionalization with molecular moieties of interest, such as small molecules, initial monomers to be used in the solid phase synthesis of oligomers, or intact oligomers. Molecular moieties of interest may be attached through cleavable sites. Derivatizing compositions for carrying out the surface functionalization process are provided as well.

15. 发明授权

US06251595B1 Methods and devices for carrying out chemical reactions 失效
公开(公告)号：US06251595B1
公开(公告)日：2001-06-26
申请号：US09100152
申请日：1998-06-18
申请人： Gary B. Gordon , Douglas J. Dellinger
发明人： Gary B. Gordon , Douglas J. Dellinger
IPC分类号： C12Q168
CPC分类号： G11C13/0019 , B01J19/0046 , B01J2219/00527 , B01J2219/00565 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00653 , B01J2219/00659 , B01J2219/00713 , B01J2219/00722 , B82Y10/00 , C07H21/00 , C12Q2565/607 , C40B40/06 , C40B70/00 , G11C13/0014
摘要： Methods and devices are disclosed for carrying out multiple chemical reactions. A plurality of electrodes supported by a semiconductor substrate is brought into proximity with a reaction medium, which comprises reagents for carrying out the chemical reactions. An item of numerical data is sent to storage means in each of a plurality of cells within the semiconductor substrate by means of a data bus. The item of numerical data is representative of an electric signal. An address is sent to address decoders in communication with the storage means. As a result, the item of numerical data is stored in the storage means. Electric signals are selectively applied to each of the electrodes by means of a plurality of digital analog converters, each electrically coupled to a respective electrode. Each of the digital analog converters is associated with a respective cell. In this way, a chemical reaction takes place proximal to and in response to the field at the electrodes to which the electric signals are selectively applied. A particular feature of the present invention is that the medium may be non-aqueous.

16. 发明授权

US09067961B2 Thiocarbon-protecting groups for RNA synthesis 有权
标题翻译：用于RNA合成的硫代保护基团
公开(公告)号：US09067961B2
公开(公告)日：2015-06-30
申请号：US13485592
申请日：2012-05-31
申请人： Douglas J. Dellinger , Agnieszka B. Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
发明人： Douglas J. Dellinger , Agnieszka B. Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
IPC分类号： C07H13/12 , C07H21/02 , C07H19/073 , C07H19/16
CPC分类号： C07H19/073 , C07H13/12 , C07H19/16 , C07H21/02 , Y02P20/55
摘要： Aspects of the invention include 2′ protected nucleoside monomers that are protected at the 2′ site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
摘要翻译：本发明的方面包括2'受保护的核苷单体，其在2'位置被硫代保护基团保护。感兴趣的硫代碳保护基包括硫代碳酸酯，硫代碳酸酯，二硫代碳酸酯基，以及硫羰氨基保护基。本发明的方面还包括包含本发明的保护基团的核酸以及使用本发明的保护基合成核酸的方法。

17. 发明授权

US08309706B2 Methods of synthesizing oligonucleotides using carbonate protecting groups and alpha-effect nucleophile deprotection 有权
标题翻译：使用碳酸酯保护基和α-效应亲核体脱保护合成寡核苷酸的方法
公开(公告)号：US08309706B2
公开(公告)日：2012-11-13
申请号：US11899828
申请日：2007-09-06
申请人： Douglas J. Dellinger , Marvin H. Caruthers , Jason R. Betley
发明人： Douglas J. Dellinger , Marvin H. Caruthers , Jason R. Betley
IPC分类号： C07H21/02 , C07H21/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20
CPC分类号： C07H19/10 , C07B2200/11 , C07H19/20 , C07H21/00 , C07H21/04 , C40B40/06 , C40B50/14 , Y02P20/55
摘要： The invention provides methods for synthesizing oligonucleotides using nucleoside monomers having carbonate protected hydroxyl groups that are deprotected with α-effect nucleophiles. The α-effect nucleophile irreversibly cleave the carbonate protecting groups while simultaneously oxidizing the internucleotide phosphite triester linkage to a phosphodiester linkage. The procedure may be carried out in aqueous solution at neutral to mildly basic pH. The method eliminates the need for separate deprotection and oxidation steps, and, since the use of acid to remove protecting groups is unnecessary, acid-induced depurination is avoided. Fluorescent or other readily detectable carbonate protecting groups can be used, enabling monitoring of individual reaction steps during oligonucleotide synthesis. The invention is particularly useful in the highly parallel, microscale synthesis of oligonucleotides.
摘要翻译：本发明提供了使用具有碳酸酯保护的羟基的核苷单体合成寡核苷酸的方法，所述羟基被α-效应亲核试剂去保护。 α效应亲核试剂不可逆地切割碳酸酯保护基，同时将磷酸三酯键的核苷酸亚磷酸三酯键连同氧化。该方法可以在中性至弱碱性pH的水溶液中进行。该方法不需要单独的脱保护和氧化步骤，并且由于不需要使用酸去除保护基团，所以避免了酸诱导的去血。可以使用荧光或其它容易检测的碳酸酯保护基团，使得能够监测寡核苷酸合成期间的各个反应步骤。本发明在寡核苷酸的高度平行，微尺度合成中特别有用。

18. 发明申请

US20100331532A1 Use Of N-Alkyl Imidazole For Sulfurization Of Oligonucleotides With An Acetyl Disulfide 有权
标题翻译：使用N-烷基咪唑用乙酰二硫化物对寡核苷酸进行硫化
公开(公告)号：US20100331532A1
公开(公告)日：2010-12-30
申请号：US12494118
申请日：2009-06-29
申请人： Agnieszka B. Sierzchala , Douglas J. Dellinger , Paul A. Metz , Victor R. Mokler
发明人： Agnieszka B. Sierzchala , Douglas J. Dellinger , Paul A. Metz , Victor R. Mokler
IPC分类号： C07H21/00 , C09K3/00
CPC分类号： C07H21/02
摘要： A method and compositions for sulfurizing at least one phosphite or thiophosphite linkage in an oligonucleotide. The addition of N-alkyl imidazole to the acetyldisulfide sulfurization solution enables the use of industrially preferred solvents or solvents that are derived from renewable resources.
摘要翻译：一种在寡核苷酸中硫化至少一种亚磷酸酯或硫代亚磷酸酯键的方法和组合物。向乙酰二硫化硫化溶液中加入N-烷基咪唑可以使用衍生自可再生资源的工业上优选的溶剂或溶剂。

19. 发明授权

US07745591B2 Phosphinoamidite carboxylates and analogs thereof in the synthesis of oligonucleotides having reduced internucleotide charge 失效
标题翻译：亚磷酰胺羧酸盐及其类似物在合成具有减少的核苷酸间电荷的寡核苷酸中
公开(公告)号：US07745591B2
公开(公告)日：2010-06-29
申请号：US11431373
申请日：2006-05-09
申请人： Douglas J. Dellinger
发明人： Douglas J. Dellinger
IPC分类号： C07H19/00 , C07H19/04 , C07H19/22
CPC分类号： C07H21/00 , C07B2200/11 , C07H19/10 , C07H19/20 , C07H21/02 , C07H21/04 , Y02P20/55
摘要： Nucleoside phosphinoamidite carboxylates and analogs are provided that have the structure of formula (III) wherein A is hydrogen, hydroxyl, lower alkoxy, lower alkoxy-substituted lower alkoxy, halogen, SH, NH2, azide or DL wherein D is O, S or NH and L is a heteroatom-protecting group, unsubstituted hydrocarbyl, substituted hydrocarbyl, heteroatom-containing hydrocarbyl, or substituted heteroatom-containing hydrocarbyl; B is a nucleobase; and one of R11 and R12 is a blocking group and the other is (IV) or (VI) in which W, X, Y, Z, R1 and n are as defined herein.
摘要翻译：提供具有式（III）结构的核苷亚磷酰胺羧酸盐和类似物，其中A是氢，羟基，低级烷氧基，低级烷氧基取代的低级烷氧基，卤素，SH，NH 2，叠氮化物或DL，其中D是O，S或NH 并且L是杂原子保护基团，未取代的烃基，取代的烃基，含杂原子的烃基或取代的含杂原子的烃基; B是核碱基; 并且R 11和R 12中的一个是阻聚基团，另一个是其中W，X，Y，Z，R 1和n如本文所定义的（IV）或（VI）。

20. 发明授权

US07524950B2 Uses of cationic salts for polynucleotide synthesis 失效
标题翻译：用于阳离子盐的多核苷酸合成
公开(公告)号：US07524950B2
公开(公告)日：2009-04-28
申请号：US11117884
申请日：2005-04-29
申请人： Douglas J. Dellinger , Eric LeProust , Bill Peck , Marvin H. Caruthers , Peter Cogan
发明人： Douglas J. Dellinger , Eric LeProust , Bill Peck , Marvin H. Caruthers , Peter Cogan
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00 , B01J2219/00378 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00675 , B01J2219/00677 , B82Y30/00 , C07B2200/11 , C07H21/02 , C07H21/04 , C40B50/14 , C40B60/14
摘要： Inkjet printhead solvents and methods of forming an addressable nucleotide array are disclosed.
摘要翻译：公开了喷墨打印头溶剂和形成可寻址核苷酸阵列的方法。

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