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11. 发明授权

US07662602B2 Polypeptides having lipase activity and polynucleotides encoding same 有权
公开(公告)号：US07662602B2
公开(公告)日：2010-02-16
申请号：US11255553
申请日：2005-10-21
申请人： Debbie Yaver , Suzanne Otani , Janine Lin , Chritopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
发明人： Debbie Yaver , Suzanne Otani , Janine Lin , Chritopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
IPC分类号： C12N9/10 , C07K1/00
CPC分类号： C12N9/20
摘要： The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

12. 发明授权

US07638616B2 Variants of beta-glucosidase 失效
标题翻译： β-葡糖苷酶的变体
公开(公告)号：US07638616B2
公开(公告)日：2009-12-29
申请号：US12147333
申请日：2008-06-26
申请人： Ana Fidantsef , Michael Lamsa , Brian Gorre-Clancy
发明人： Ana Fidantsef , Michael Lamsa , Brian Gorre-Clancy
IPC分类号： C07H21/04 , C12N1/20 , C12N1/21
CPC分类号： C12N9/2445 , A61K36/062 , C12P19/02 , C12P19/14 , C12Q1/6895 , C12Y302/01021
摘要： The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译：本发明涉及亲本β-葡糖苷酶的变体，其包含在对应于SEQ ID NO：2的氨基酸1至842的位置142,183,266和703的一个或多个位置处的取代或对应于位置142， SEQ ID NO：70的氨基酸1至844的183,266和705，其中所述变体具有β-葡糖苷酶活性。本发明还涉及编码变体β-葡糖苷酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

13. 发明授权

US09045748B2 Methods for transforming and expression screening of filamentous fungal cells with a DNA library 有权
标题翻译：用DNA文库转化和表达筛选丝状真菌细胞的方法
公开(公告)号：US09045748B2
公开(公告)日：2015-06-02
申请号：US11140150
申请日：2005-05-26
申请人： Sarah Teter , Michael Lamsa , Joel Cherry , Connie Ward
发明人： Sarah Teter , Michael Lamsa , Joel Cherry , Connie Ward
IPC分类号： C12N15/10 , C12N15/80 , C40B40/02
CPC分类号： C12N15/1037 , C12N15/1086 , C12N15/80 , C40B40/02
摘要： The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides. The present invention also relates to isolated polynucleotides encoding polypeptides of interest obtained by such methods, to nucleic acid constructs, expression vectors, and recombinant host cells comprising the isolated polynucleotides, and to methods of producing the polypeptides encoded by the isolated polynucleotides.
摘要翻译：本发明涉及丝状真菌转化体的表达筛选方法，其包括：（a）分离导入大肠杆菌的DNA文库的单个菌落转化体; （b）从每个单个菌落的大肠杆菌转化体制备DNA; （c）将步骤（b）的每种DNA制剂的样品引入到丝状真菌的原生质体的分离悬浮液中以获得其转化体，其中每个转化体含有来自DNA文库的单个多核苷酸的一个或多个拷贝; （d）在选择性生长培养基上生长步骤（c）的单个丝状真菌转化体，从而允许丝状真菌转化体生长，同时抑制未转化的丝状真菌的生长; 和（e）测量由各个多核苷酸编码的每种多肽的活性或性质。本发明还涉及通过这些方法获得的编码感兴趣的多肽的分离的多核苷酸，包括分离的多核苷酸的核酸构建体，表达载体和重组宿主细胞，以及由分离的多核苷酸编码的多肽的制备方法。

14. 发明授权

US08377675B2 Polypeptides having lipase activity and polynucleotides encoding same 失效
标题翻译：具有脂肪酶活性的多肽和编码该多肽的多核苷酸
公开(公告)号：US08377675B2
公开(公告)日：2013-02-19
申请号：US12696676
申请日：2010-01-29
申请人： Suzanne Otani , Debbie Yaver , Haiyan Ge , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
发明人： Suzanne Otani , Debbie Yaver , Haiyan Ge , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
IPC分类号： C12N9/20 , C11D3/386 , C12N15/55
CPC分类号： C12N9/20 , C12Y301/01003
摘要： The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
摘要翻译：本发明涉及具有脂肪酶活性的分离的多肽和编码多肽的分离的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及生产和使用多肽的方法。

15. 发明授权

US07855172B2 Polypeptides having lipase activity and polynucleotides encoding same 有权
公开(公告)号：US07855172B2
公开(公告)日：2010-12-21
申请号：US12687696
申请日：2010-01-14
申请人： Debbie Yaver , Suzanne Otani , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
发明人： Debbie Yaver , Suzanne Otani , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
IPC分类号： C11D3/386 , C07K1/00 , C12N9/10
CPC分类号： C12N9/20
摘要： The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

16. 发明授权

US07666630B2 Polypeptides having lipase activity and polynucleotides encoding same 失效
公开(公告)号：US07666630B2
公开(公告)日：2010-02-23
申请号：US11241677
申请日：2005-09-30
申请人： Debbie Yaver , Suzanne Otani , Haiyan Ge , Janine Lin , Chritopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
发明人： Debbie Yaver , Suzanne Otani , Haiyan Ge , Janine Lin , Chritopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
IPC分类号： C12N15/54 , C12N9/20 , C12N15/11
CPC分类号： C12N9/20 , C12Y301/01003
摘要： The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

17. 发明申请

US20080020425A1 Polypeptides having lipase activity and polynucleotides encoding same 有权
标题翻译：具有脂肪酶活性的多肽和编码该多肽的多核苷酸
公开(公告)号：US20080020425A1
公开(公告)日：2008-01-24
申请号：US11255553
申请日：2005-10-21
申请人： Debbie Yaver , Suzanne Otani , Janine Lin , Chritopher Amolo , Kim Borch , Shamkant Anant , Michael Lamsa , Barbara Cherry
发明人： Debbie Yaver , Suzanne Otani , Janine Lin , Chritopher Amolo , Kim Borch , Shamkant Anant , Michael Lamsa , Barbara Cherry
IPC分类号： C12P21/04 , A01H5/00 , C11D3/386 , C12N15/11 , C12N5/06 , C12N9/14
CPC分类号： C12N9/20
摘要： The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
摘要翻译：本发明涉及具有脂肪酶活性的分离的多肽和编码多肽的分离的多核苷酸。本发明还涉及核酸构建体，载体，和包含所述多核苷酸的宿主细胞以及方法用于产生和使用所述多肽。

18. 发明申请

US20060172379A1 Methods for transforming and expression screening of filamentous fungal cells with a DNA library 有权
公开(公告)号：US20060172379A1
公开(公告)日：2006-08-03
申请号：US11140150
申请日：2005-05-26
申请人： Sarah Teter , Michael Lamsa , Joel Cherry , Connie Ward
发明人： Sarah Teter , Michael Lamsa , Joel Cherry , Connie Ward
IPC分类号： C12P21/06 , C12N1/16 , C12N15/74
CPC分类号： C12N15/1037 , C12N15/1086 , C12N15/80 , C40B40/02
摘要： The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides. The present invention also relates to isolated polynucleotides encoding polypeptides of interest obtained by such methods, to nucleic acid constructs, expression vectors, and recombinant host cells comprising the isolated polynucleotides, and to methods of producing the polypeptides encoded by the isolated polynucleotides.

19. 发明授权

US08852913B2 Variants of beta-glucosidases 有权
标题翻译： β-葡糖苷酶的变体
公开(公告)号：US08852913B2
公开(公告)日：2014-10-07
申请号：US12639642
申请日：2009-12-16
申请人： Ana Fidantsef , Michael Lamsa , Brian Gorre-Clancy
发明人： Ana Fidantsef , Michael Lamsa , Brian Gorre-Clancy
IPC分类号： C12N9/12 , C07K14/00 , C07H21/04 , C12N1/21
CPC分类号： C12N9/2445 , A61K36/062 , C12P19/02 , C12P19/14 , C12Q1/6895 , C12Y302/01021
摘要： The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译：本发明涉及亲本β-葡糖苷酶的变体，其包含在对应于SEQ ID NO：2的氨基酸1至842的位置142,183,266和703的一个或多个位置处的取代或对应于位置142， SEQ ID NO：70的氨基酸1至844的183,266和705，其中所述变体具有β-葡糖苷酶活性。本发明还涉及编码变体β-葡糖苷酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

20. 发明申请

US20110053822A1 Polypeptides having lipase activity and polynucleotides encoding same 有权
公开(公告)号：US20110053822A1
公开(公告)日：2011-03-03
申请号：US12944557
申请日：2010-11-11
申请人： Debbie Yaver , Suzanne Otani , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
发明人： Debbie Yaver , Suzanne Otani , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
IPC分类号： C11D3/386 , C12N9/20
CPC分类号： C12N9/20
摘要： The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

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