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11. 发明授权

US06855545B1 Indexed library of cells containing genomic modifications and methods of making and utilizing the same 有权
标题翻译：指定的含有基因组修饰的细胞库，以及制备和利用它们的方法
公开(公告)号：US06855545B1
公开(公告)日：2005-02-15
申请号：US09639453
申请日：2000-08-15
申请人： Arthur T. Sands , Glenn A. Friedrich , Brian Zambrowicz , Allan Bradley
发明人： Arthur T. Sands , Glenn A. Friedrich , Brian Zambrowicz , Allan Bradley
IPC分类号： C12N15/10 , C12N15/85 , C12N15/867 , C12N15/86 , C12N15/00 , C12N15/09 , C12N15/82 , C12Q1/68
CPC分类号： C12N15/1082 , A01K2217/075 , C12N15/102 , C12N15/85 , C12N15/86 , C12N2740/10043 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Methods and vectors (both DNA and retroviral) are provided for the construction of a Library of mutated cells. The Library will preferably contain mutations in essentially all genes present in the gene of the cells. The nature of the Library and the vectors allow for methods of screening for mutations in specific genes, and for gathering nucleotide sequence data from each mutated gene to provide a database of tagged gene sequences. Such a database provides a means to access the individual mutant cell clones contained in the Library. The invention includes the described Library, methods of making the same, and vectors used to construct the Library. Methods are also provided for accessing individual parts of the Library either by sequence or by pooling and screening. The invention also provides for the generation of non-human transgenic animals which are mutant for specific genes as isolated and generated from the cells of the Library.
摘要翻译：提供方法和载体（DNA和逆转录病毒）用于构建突变细胞库。该文库将优选地包含基因中存在于细胞基因中的所有基因的突变。图书馆和载体的性质允许筛选特定基因突变的方法，并收集每个突变基因的核苷酸序列数据，以提供标记基因序列的数据库。这样的数据库提供了访问图书馆中包含的各个突变细胞克隆的方法。本发明包括描述的库，制作方法和用于构建库的向量。还提供了通过序列或汇总和筛选来访问图书馆的各个部分的方法。本发明还提供了从文库细胞分离和产生的特异性基因突变的非人转基因动物的产生。

12. 发明授权

US6048695A Chemically modified nucleic acids and methods for coupling nucleic acids to solid support 失效
标题翻译：化学修饰的核酸和将核酸耦合到固体支持物的方法
公开(公告)号：US6048695A
公开(公告)日：2000-04-11
申请号：US71876
申请日：1998-05-04
申请人： Allan Bradley , Wei Wen Cai
发明人： Allan Bradley , Wei Wen Cai
IPC分类号： G01N33/53 , C07B61/00 , C07H21/00 , C07H23/00 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N37/00
CPC分类号： C12Q1/6806 , C07H21/00 , C07B2200/11 , C12Q1/6837 , C12Q2525/197 , C40B40/00
摘要： The invention relates to novel chemically modified nucleic acids with enhanced lability towards solid supports, such as glass. These modified nucleic acids can be readily affixed to solid supports, for instance, a glass surface, without first derivatizing the glass surface. High-density microarrays based on these modified nucleic acids as well as methods for preparing these microarrays are also useful.
摘要翻译：本发明涉及对固体支持物例如玻璃具有增强的不稳定性的新化学修饰的核酸。这些修饰的核酸可以容易地固定到固体支持物，例如玻璃表面，而不首先使玻璃表面衍生化。基于这些修饰的核酸的高密度微阵列以及制备这些微阵列的方法也是有用的。

13. 发明授权

US5569824A Transgenic mice containing a disrupted p53 gene 失效
标题翻译：含有破坏的p53基因的转基因小鼠
公开(公告)号：US5569824A
公开(公告)日：1996-10-29
申请号：US278588
申请日：1994-07-21
申请人： Lawrence A. Donehower , Allan Bradley , Janet S. Butel , Betty Slagle
发明人： Lawrence A. Donehower , Allan Bradley , Janet S. Butel , Betty Slagle
IPC分类号： C07K14/47 , C12N15/85 , C12N5/00 , A61K49/00 , C12N15/00
CPC分类号： C12N15/8509 , A01K67/0276 , A01K67/0278 , C07K14/4746 , A01K2207/15 , A01K2217/00 , A01K2217/075 , A01K2227/105 , A01K2267/0331
摘要： A desired non-human animal or an animal cell or human cell which contains a predefined, specific and desired alteration in at least one of its two p53 chromosomal alleles, such that at least one of these alleles contains a mutation which alters the expression of the allele, and the other of the alleles expresses either a normal p53 gene product, or comprises an identical or different p53 mutation.
摘要翻译：一种期望的非人动物或动物细胞或人细胞，其在其两个p53染色体等位基因中的至少一个中包含预定义的，特异性和期望的改变，使得这些等位基因中的至少一个含有改变等位基因，另一个等位基因表达正常的p53基因产物，或包含相同或不同的p53突变。

14. 发明授权

US06975943B2 Clone-array pooled shotgun strategy for nucleic acid sequencing 失效
标题翻译：用于核酸测序的克隆阵列合并霰弹枪策略
公开(公告)号：US06975943B2
公开(公告)日：2005-12-13
申请号：US09969111
申请日：2001-09-24
申请人： Richard Gibbs , Allan Bradley , Wei-Wen Cai
发明人： Richard Gibbs , Allan Bradley , Wei-Wen Cai
IPC分类号： C12N15/10 , C12Q1/68 , G06F19/00 , G01N33/48
CPC分类号： C12N15/1034 , C12Q1/6874
摘要： A simplified strategy for sequencing large genomes has been developed. Clone-Array Pooled Shotgun Sequencing (CAPSS) is based upon pooling rows and columns of arrayed genomic clones, for shotgun library construction. Random sequences are accumulated and the data are assembled by sequential comparison of rows and columns, to resolve the sequence of clones at points of intersection. Compared to either a clone-by-clone approach or whole genome shotgun sequencing, CAPSS requires relatively few library constructions and only minimal computational power for a complete genome assembly. The strategy is suitable for sequencing large genomes for which there are no sequence-ready maps, but for which relatively high resolution STS maps and highly redundant BAC libraries are available. It is immediately applicable to the sequencing of mouse, rat, zebra fish and other important genomes, and can be managed in a cooperative fashion to take advantage of the distributed international DNA sequencing capacity.
摘要翻译：已经开发了用于测序大基因组的简化策略。克隆阵列池式霰弹枪测序（CAPSS）基于汇集排列的基因组克隆的行和列，用于霰弹枪库构建。随机序列被累积，并且数据通过行和列的顺序比较来组装，以解决交叉点处的克隆序列。与克隆克隆方法或全基因组鸟枪法测序相比，CAPSS需要相对较少的文库构建，并且对于完整的基因组装配仅需要最小的计算能力。该策略适用于对没有序列准备图的大型基因组进行排序，但对于相对较高分辨率的STS图和高度冗余的BAC库可用。它可以立即适用于小鼠，大鼠，斑马鱼等重要基因组的测序，可以以合作的方式管理，以利用分布式国际DNA测序能力。

15. 发明申请

US20050095620A1 Indexed library of cells containing genomic modifications and methods of making and utilizing the same 有权
标题翻译：指定的含有基因组修饰的细胞库，以及制备和利用它们的方法
公开(公告)号：US20050095620A1
公开(公告)日：2005-05-05
申请号：US10917273
申请日：2004-08-11
申请人： Brian Zambrowicz , Glenn Friedrich , Allan Bradley , Arthur Sands
发明人： Brian Zambrowicz , Glenn Friedrich , Allan Bradley , Arthur Sands
IPC分类号： C12N15/10 , C12N15/85 , C12N15/867 , C12Q1/68 , A01K67/027 , C12N5/06 , G01N33/53 , G01N33/567
CPC分类号： C12N15/86 , A01K2217/075 , C12N15/102 , C12N15/1051 , C12N15/1065 , C12N15/1086 , C12N15/85 , C12N2740/13043 , C12N2800/60 , C12N2840/203 , C12N2840/44
摘要： Methods and vectors (both DNA and retroviral) are provided for the construction of a Library of mutated cells. The Library will preferably contain mutations in essentially all genes present in the genome of the cells. The nature of the Library and the vectors allow for methods of screening for mutations in specific genes, and for gathering nucleotide sequence data from each mutated gene to provide a database of tagged gene sequences. Such a database provides a means to access the individual mutant cell clones contained in the Library. The invention includes the described Library, methods of making the same, and vectors used to construct the Library. Methods are also provided for accessing individual parts of the Library either by sequence or by pooling and screening. The invention also provides for the generation of non-human transgenic animals which are mutant for specific genes as isolated and generated from the cells of the Library.
摘要翻译：提供方法和载体（DNA和逆转录病毒）用于构建突变细胞库。该文库优选地在基因组中存在基因组中所有基因的突变。图书馆和载体的性质允许筛选特定基因突变的方法，并收集每个突变基因的核苷酸序列数据，以提供标记基因序列的数据库。这样的数据库提供了访问图书馆中包含的各个突变细胞克隆的方法。本发明包括描述的库，制作方法和用于构建库的向量。还提供了通过序列或汇总和筛选来访问图书馆的各个部分的方法。本发明还提供了从文库细胞中分离并产生的特异性基因突变的非人转基因动物的产生。

16. 发明申请

US20060275787A1 Compositions and methods for array-based genomic nucleic acid analysis of biological molecules 审中-公开
标题翻译：用于基于阵列的生物分子基因组核酸分析的组合物和方法
公开(公告)号：US20060275787A1
公开(公告)日：2006-12-07
申请号：US11319752
申请日：2005-12-27
申请人： Allan Bradley , Wei-Wen Cai , Upendra Marathi
发明人： Allan Bradley , Wei-Wen Cai , Upendra Marathi
IPC分类号： C12Q1/68 , G01N33/53 , C12M1/34 , C07H21/04
CPC分类号： C07H21/00 , B01J2219/00369 , B01J2219/00497 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00722 , C07B2200/11 , C07H21/04 , C12Q1/6806 , C12Q1/6837 , C12Q2525/197 , C40B40/00
摘要： The invention provides biological molecules modified by reaction with a compound having the formula: R1—X—R2 , wherein R1 is a cyclic ether group or an amino group, R2 is an alkoxysilane group and X is a moiety chemically suitable for linking the cyclic ether group or the amino group to the alkoxysilane group. The invention also provides arrays, or “biochips,” comprising these modified biological molecules. Also provided are methods for making and using these compositions.
摘要翻译：本发明提供通过与具有下式的化合物反应而修饰的生物分子：R 1 -XR 2 N，其中R 1是环醚基或氨基，R 2是烷氧基硅烷基，X是化学上适合于将环醚基或氨基连接到烷氧基硅烷基的部分。本发明还提供包含这些修饰的生物分子的阵列或“生物芯片”。还提供了制备和使用这些组合物的方法。

17. 发明申请

US20050064494A1 Chemically modified biological molecules and methods for coupling biological molecules to solid support 审中-公开
标题翻译：化学修饰的生物分子和将生物分子与固体支持物偶联的方法
公开(公告)号：US20050064494A1
公开(公告)日：2005-03-24
申请号：US10948858
申请日：2004-09-24
申请人： Allan Bradley , Wei-Wen Cai
发明人： Allan Bradley , Wei-Wen Cai
IPC分类号： G01N33/53 , C07B61/00 , C07H21/00 , C07H23/00 , C12M1/00 , C12N15/09 , C12Q1/68 , C12Q1/6806 , C12Q1/6837 , G01N37/00 , C12M1/34
CPC分类号： C12Q1/6806 , C07B2200/11 , C07H21/00 , C12Q1/6837 , C12Q2525/197 , C40B40/00
摘要： The invention relates to novel chemically modified biological molecules with enhanced lability towards solid supports, such as glass. These modified molecules can be readily affixed to solid supports, for instance, a glass surface, without first derivatizing the glass surface. High-density microarrays based on these modified molecules as well as methods for preparing these microarrays are also useful.
摘要翻译：本发明涉及对固体支持物（例如玻璃）具有增强的不稳定性的新的化学修饰的生物分子。这些改性分子可以容易地固定到固体支持物，例如玻璃表面，而不首先使玻璃表面衍生化。基于这些修饰分子的高密度微阵列以及制备这些微阵列的方法也是有用的。

18. 发明授权

US6136566A Indexed library of cells containing genomic modifications and methods of making and utilizing the same 失效
公开(公告)号：US6136566A
公开(公告)日：2000-10-24
申请号：US726867
申请日：1996-10-04
申请人： Arthur Sands , Glenn Friedrich , Brian Zambrowicz , Allan Bradley
发明人： Arthur Sands , Glenn Friedrich , Brian Zambrowicz , Allan Bradley
IPC分类号： C12N15/10 , C12N15/85 , C12N15/867 , C12P21/00 , C07H21/04 , C12N5/00 , C12N15/63
CPC分类号： C12N15/86 , C12N15/102 , C12N15/1034 , C12N15/85 , A01K2217/075 , C12N2740/10043 , C12N2800/60 , C12N2840/44
摘要： Methods and vectors (both DNA and retroviral) are provided for the construction of a Library of mutated cells. The Library will preferably contain mutations in essentially all genes present in the genome of the cells. The nature of the Library and the vectors allow for methods of screening for mutations in specific genes, and for gathering nucleotide sequence data from each mutated gene to provide a database of tagged gene sequences. Such a database provides a means to access the individual mutant cell clones contained in the Library. The invention includes the described Library, methods of making the same, and vectors used to construct the Library. Methods are also provided for accessing individual parts of the Library either by sequence or by pooling and screening. The invention also provides for the generation of non-human transgenic animals which are mutant for specific genes as isolated and generated from the cells of the Library.

19. 发明授权

US5948952A Xeroderma pigmentosum-deficient mouse 失效
标题翻译：皮肤色素沉着缺陷小鼠
公开(公告)号：US5948952A
公开(公告)日：1999-09-07
申请号：US708958
申请日：1996-09-06
申请人： Arthur T. Sands , Alejandro Abuin , Allan Bradley
发明人： Arthur T. Sands , Alejandro Abuin , Allan Bradley
IPC分类号： C07K14/72 , C12N15/85 , C12N5/00 , A61K49/00 , C12N5/06 , G01N33/567
CPC分类号： C12N15/8509 , A01K67/0276 , C07K14/721 , A01K2217/075 , A01K2227/105 , A01K2267/0306
摘要： An Xeroderma pigmentosum-deficient mouse, as well as methods for screening for the damaging and tumorigenic effect of ultraviolet light or a chemical DNA damaging agent, are disclosed.
摘要翻译：公开了一种皮肤色素沉着小鼠，以及用于筛选紫外线或化学DNA损伤剂的损伤和致瘤作用的方法。

20. 发明申请

US20090149336A1 Indexed library of cells containing genomic modifications and methods of making and utilizing the same 审中-公开
标题翻译：指定的含有基因组修饰的细胞库，以及制备和利用它们的方法
公开(公告)号：US20090149336A1
公开(公告)日：2009-06-11
申请号：US11978961
申请日：2007-10-29
申请人： Brian Zambrowicz , Glenn A. Friedrich , Allan Bradley , Arthur T. Sands
发明人： Brian Zambrowicz , Glenn A. Friedrich , Allan Bradley , Arthur T. Sands
IPC分类号： C40B30/04
CPC分类号： C12N15/1082 , A01K2217/075 , C12N15/102 , C12N15/1034 , C12N15/1051 , C12N15/85 , C12N15/86 , C12N2740/10043 , C12N2800/60 , C12N2840/203 , C12N2840/44
摘要： Methods and vectors (both DNA and retroviral) are provided for the construction of a Library of mutated cells. The Library will preferably contain mutations in essentially all genes present in the genome of the cells. The nature of the Library and the vectors allow for methods of screening for mutations in specific genes, and for gathering nucleotide sequence data from each mutated gene to provide a database of tagged gene sequences. Such a database provides a means to access the individual mutant cell clones contained in the Library. The invention includes the described Library, methods of making the same, and vectors used to construct the Library. Methods are also provided for accessing individual parts of the Library either by sequence or by pooling and screening. The invention also provides for the generation of non-human transgenic animals which are mutant for specific genes as isolated and generated from the cells of the Library.
摘要翻译：提供方法和载体（DNA和逆转录病毒）用于构建突变细胞库。该文库优选地在基因组中存在基因组中所有基因的突变。图书馆和载体的性质允许筛选特定基因突变的方法，并收集每个突变基因的核苷酸序列数据，以提供标记基因序列的数据库。这样的数据库提供了访问图书馆中包含的各个突变细胞克隆的方法。本发明包括描述的库，制作方法和用于构建库的向量。还提供了通过序列或汇总和筛选来访问图书馆的各个部分的方法。本发明还提供了从文库细胞中分离并产生的特异性基因突变的非人转基因动物的产生。

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