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11. 发明专利

CA2728746A1 METHODS OF AMPLIFYING AND SEQUENCING NUCLEIC ACIDS 未知
公开(公告)号：CA2728746A1
公开(公告)日：2005-01-13
申请号：CA2728746
申请日：2004-01-28
申请人： 454 CORP
发明人： LEAMON JOHN H , LOHMAN KENTON , ROTHBERG JONATHAN M , WEINER MICHAEL P
IPC分类号： C12Q1/6874 , B01L3/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/686 , C40B20/00 , C40B20/04 , C40B50/06 , C40B50/14 , G01N21/25 , G01N21/64
摘要： A method for sequencing nucleic acids. The method includes fragmenting large template nucleic acid molecules to generate a plurality of fragmented nucleic acids; delivering the fragmented nucleic acids into aqueous microreactors in a water-in-oil emulsion such that a plurality of aqueous microreactors comprise a single copy of a fragmented nucleic acid, a single bead capable of binding to the fragmented nucleic acid, and amplification reaction solution containing reagents necessary to perform nucleic acid amplification; amplifying the fragmented nucleic acids in the microreactors to form amplified copies of said nucleic acids and binding the amplified copies to beads in the microreactors; delivering the beads to areas on a planar surface to produce an array of at least 10,000 reaction sites; and, performing a sequencing reaction simultaneously on the nucleic acids at the sites.

12. 发明专利

AU2004209001A1 Bead emulsion nucleic acid amplification 未知
公开(公告)号：AU2004209001A1
公开(公告)日：2004-08-19
申请号：AU2004209001
申请日：2004-01-28
申请人： 454 CORP
发明人： CHEN YI-JU , LEAMON JOHN H , LEFKOWITZ STEVEN , LOHMAN KENTON , MAKHIJANI VINOD , SARKIS GARY J , ROTHBERG JONATHAN , WEINER MICHAEL , SRINIVASAN MAITHREYAN , BERKA JAN
IPC分类号： B01L3/00 , C07H21/00 , C12N15/10 , C12P19/34 , G01N21/25 , G01N21/64 , C12Q1/68
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

13. 发明专利

CA2513899C METHODS OF AMPLIFYING AND SEQUENCING NUCLEIC ACIDS 未知
公开(公告)号：CA2513899C
公开(公告)日：2013-03-26
申请号：CA2513899
申请日：2004-01-28
申请人： 454 CORP
发明人： LEAMON JOHN H , LOHMAN KENTON , ROTHBERG JONATHAN M , WEINER MICHAEL P
IPC分类号： C12Q1/68 , B01L3/00 , C07H21/00 , C12N15/10 , C12P19/34 , C40B20/00 , C40B40/06 , C40B50/06 , C40B50/14 , G01N21/25 , G01N21/64
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

14. 发明公开

EP1594980A4 BEAD EMULSION NUCLEIC ACID AMPLIFICATION 有权转让
标题翻译：核酸扩增基于BEAD EMULSION
公开(公告)号：EP1594980A4
公开(公告)日：2007-05-02
申请号：EP04706051
申请日：2004-01-28
申请人： 454 CORP
发明人： BERKA JAN , CHEN YI-JU , LEAMON JOHN H , LEFKOWITZ STEVEN , LOHMAN KENTON , MAKHIJANI VINOD , SARKIS GARY J , ROTHBERG JONATHAN , WEINER MICHAEL , SRINIVASAN MAITHREYAN
IPC分类号： C12N15/10 , B01L3/00 , C07H21/00 , C12P19/34 , C12Q1/68 , G01N21/25 , G01N21/64
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

15. 发明专利

JP2010142233A Methods of amplifying and sequencing nucleic acids 审中-公开
标题翻译：放大和测序核酸的方法
公开(公告)号：JP2010142233A
公开(公告)日：2010-07-01
申请号：JP2009297745
申请日：2009-12-28
申请人： 454 コーポレーション , 454 Corp
发明人： LEAMON JOHN H , LOHMAN KENTON , ROTHBERG JONATHAN M , WEINER MICHAEL P
IPC分类号： C12Q1/68 , B01L3/00 , C07H21/00 , C12M1/00 , C12N15/09 , C12N15/10 , C12P19/34 , G01N21/25 , G01N21/64 , G01N37/00
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： PROBLEM TO BE SOLVED: To provide an apparatus and method for performing rapid DNA sequencing, such as genomic sequencing. SOLUTION: The method for sequencing a nucleic acid includes the steps of fragmenting large template nucleic acid molecules to generate a plurality of fragmented nucleic acids, delivering the fragmented nucleic acids into aqueous microreactors in a water-in-oil emulsion, amplifying the fragmented nucleic acids in the microreactors to form amplified copies of the nucleic acids and binding the amplified copies to beads in the microreactors, delivering the beads to an array of at least 10,000 reaction chambers on a planar surface, wherein a plurality of the reaction chambers include no more than a single bead, and performing a sequencing reaction simultaneously on a plurality of the reaction chambers. Also provided is the method therefor. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供用于进行快速DNA测序的装置和方法，例如基因组测序。解决方案：用于对核酸进行测序的方法包括将大模板核酸分子碎裂以产生多个片段化的核酸的步骤，将片段化的核酸在油包水乳液中递送到水性微量反应器中，在微反应器中形成片段化核酸以形成核酸的扩增拷贝并将扩增的拷贝结合到微反应器中的珠粒，将珠粒递送至平面上至少10,000个反应室的阵列，其中多个反应室包括不超过单个珠，并且在多个反应室上同时进行测序反应。还提供了其方法。版权所有（C）2010，JPO＆INPIT

16. 发明专利

CA2727850C METHODS OF AMPLIFYING AND SEQUENCING NUCLEIC ACIDS 未知
公开(公告)号：CA2727850C
公开(公告)日：2013-04-30
申请号：CA2727850
申请日：2004-01-28
申请人： 454 CORP
发明人： LEAMON JOHN H , LOHMAN KENTON , ROTHBERG JONATHAN M , WEINER MICHAEL P
IPC分类号： C12Q1/68 , B01L3/00 , C07H21/00 , C12N15/10 , C12P19/34 , C40B40/06 , C40B50/06 , C40B50/14 , G01N21/25 , G01N21/64
摘要： A method for sequencing nucleic acids. The method includes fragmenting large template nucleic acid molecules to generate a plurality of fragmented nucleic acids; attaching one strand of a plurality of the fragmented nucleic acids individually to beads to generate single stranded nucleic acids attached individually to beads; delivering a population of the single stranded fragmented nucleic acids attached individually to beads to an array of at least 10,000 reaction chambers on a planar surface, wherein a plurality of the reaction chambers comprise no more than one bead with one single stranded fragmented nucleic acid; amplifying the single stranded nucleic acids in the reaction chambers; and, performing a sequencing reaction simultaneously on a plurality of the reaction chambers.

17. 发明专利

ES2380893T3 Amplificación de ácidos nucleicos en emulsión en perlas 未知
公开(公告)号：ES2380893T3
公开(公告)日：2012-05-21
申请号：ES09175588
申请日：2004-01-28
申请人： 454 CORP , 454 LIFE SCIENCES CORP
发明人： BERKA JAN , CHEN YI-JU , LEAMON JOHN H , LEFKOWITZ STEVEN , LOHMAN KENTON , MAKHIJANI VINOD , SARKIS GARY J , ROTHBERG JONATHAN , WEINER MICHAEL , SRINIVASAN MAITHREYAN
IPC分类号： C12N15/10 , B01L3/00 , C07H21/00 , C12P19/34 , C12Q1/68 , G01N21/25 , G01N21/64
摘要： Un metodo para enriquecer un amplicón, que comprende: (a) distribuir una solución que comprende una pluralidad de perlas y una pluralidad de especies de moleculas de acido nucleico de molde, en una pluralidad de gotitas de emulsión acuosa en una fase oleosa termoestable; en el que un primer subconjunto de las gotitas comprenden una o mas de las perlas y una o mas de las especies de acido nucleico de molde encapsuladas en ellas, y un segundo subconjunto de las gotitas comprenden una o mas de las perlas sin ninguna de las especies de moleculas de acido nucleico de molde encapsuladas en ellas; (b) amplificar las especies de moleculas de acido nucleico de molde del primer subconjunto de gotitas, en el que una o mas perlas del primer subconjunto de gotitas acuosas tienen un amplicón inmovilizado en ellas; (c) romper las gotitas de emulsión acuosa para liberar las perlas del primer y el segundo subconjuntos; (d) hibridar con el amplicón inmovilizado en las perlas un cebador que es complementario del extremo 3' del amplicón; y o bien: (e) (i) formar un complejo de perla con amplicón inmovilizado y perla de enriquecimiento, en el que la perla de enriquecimiento y el cebador hibridado forman un par de captura-diana, y en el que las perlas de enriquecimiento pueden ser aisladas bajo una condición selectiva; y (f) (i) aplicar la condición selectiva para aislar el complejo de perla con amplicón inmovilizado y perla de enriquecimiento, desde las perlas que no tienen amplicón unido; o bien (e) (ii) exponer las perlas con amplicón inmovilizado a una o mas superficies de unión que comprenden restos de captura, cuyos restos de captura forman pares de captura-diana con el cebador hibridado de las perlas con amplicón inmovilizado, y separar las perlas con amplicón inmovilizado desde las perlas que no tienen amplicón unido.

18. 发明专利

AT448302T 未知
公开(公告)号：AT448302T
公开(公告)日：2009-11-15
申请号：AT04706051
申请日：2004-01-28
申请人： 454 CORP
发明人： BERKA JAN , CHEN YI-JU , LEAMON JOHN , LEFKOWITZ STEVEN , LOHMAN KENTON , MAKHIJANI VINOD , SARKIS GARY , ROTHBERG JONATHAN , WEINER MICHAEL , SRINIVASAN MAITHREYAN
IPC分类号： C12N15/10 , B01L3/00 , C07H21/00 , C12P19/34 , C12Q1/68 , G01N21/25 , G01N21/64
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

19. 发明专利

AT437945T 未知
公开(公告)号：AT437945T
公开(公告)日：2009-08-15
申请号：AT04785758
申请日：2004-01-28
申请人： 454 CORP
发明人： LEAMON JOHN , LOHMAN KENTON , ROTHBERG JONATHAN , WEINER MICHAEL
IPC分类号： C12N15/10 , B01L3/00 , C07H21/00 , C12P19/34 , C12Q1/68 , G01N21/25 , G01N21/64
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

20. 发明专利

CA2513899A1 METHODS OF AMPLIFYING AND SEQUENCING NUCLEIC ACIDS 未知
公开(公告)号：CA2513899A1
公开(公告)日：2005-01-13
申请号：CA2513899
申请日：2004-01-28
申请人： 454 CORP
发明人： WEINER MICHAEL P , ROTHBERG JONATHAN M , LEAMON JOHN H , LOHMAN KENTON
IPC分类号： B01L3/00 , C07H21/00 , C12N15/10 , C12P19/34 , G01N21/25 , G01N21/64 , C12Q1/68 , C12M1/36 , C12M1/40
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

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