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11. 发明授权

KR100435143B1 식물조직의 특이 프로모터 영역 有权
标题翻译： 식물조직의특이프로터영역
公开(公告)号：KR100435143B1
公开(公告)日：2004-06-14
申请号：KR1020010012040
申请日：2001-03-08
申请人： 대한민국(농촌진흥청장)
发明人： 박범석 , 진용문 , 김호일 , 이연희 , 김현욱
IPC分类号： C12N15/11
摘要： PURPOSE: Provided are plant tissue specific promoters, thereby controlling when, where and how much to express a foreign gene. CONSTITUTION: A pollen specific promoter region Pban102 contains 1,177 bp upstream of an initiation codon ATG in a genomic clone of BAN102 gene having the nucleotide sequence of SEQ ID NO: 1. A pollen superior promoter region Pban103 contains 1 to 1831 bp in a genomic clone of BAN103 gene having the nucleotide sequence of SEQ ID NO: 2. A pollen specific promoter region P84 contains 1 to 565 bp in a genomic clone of BAN84 gene having SEQ ID NO: 3. Plasmids, pGBAN84, p84Xb, p84Bm and p84SM, contain the genomic clone of Chinese cabbage containing the BAN84 gene. A carpet tissue specific promoter region PBcA9 contains 616 to 1,436 bp in a genomic clone of BcA9 gene having the nucleotide sequence of SEQ ID NO: 4. A vascular bundle specific promoter region Pbif38 contains 1 to 966 bp in a gemonic clone of BIF38 gene having the nucleotide sequence of SEQ ID NO: 5.
摘要翻译：目的：提供植物组织特异性启动子，从而控制表达外源基因的时间，地点和数量。构成：花粉特异性启动子区Pban102含有具有SEQ ID NO：1的核苷酸序列的BAN102基因的基因组克隆中起始密码子ATG的上游1,177bp。花粉上位启动子区Pban103在基因组克隆中含有1至1831bp 具有SEQ ID NO：2的核苷酸序列的BAN103基因。在具有SEQ ID NO：3的BAN84基因的基因组克隆中，花粉特异性启动子区P84含有1至565bp。质粒pGBAN84，p84Xb，p84Bm和p84SM含有含有BAN84基因的大白菜基因组克隆。在具有SEQ ID NO：4的核苷酸序列的BcA9基因的基因组克隆中，地毯组织特异性启动子区域PBcA9含有616至1,436bp。维管束特异性启动子区域Pbif38在BIF38基因的基因克隆中含有1至966bp， SEQ ID NO：5的核苷酸序列。

12. 发明授权

KR100375675B1 배추과 작물의 형질전환을 위한 합성 곤충 독소 유전자 有权
标题翻译： 배추과작물의형질전환을위소합곤충곤충독소유전자
公开(公告)号：KR100375675B1
公开(公告)日：2003-03-15
申请号：KR1020000046327
申请日：2000-08-10
申请人： 대한민국(농촌진흥청장)
发明人： 박범석 , 조현석 , 진용문 , 김호일
IPC分类号： C12N15/33
摘要： PURPOSE: A synthetic insecticidal toxin gene for the transformation of Brassicae plants is provided, thereby effectively preventing plants from being damaged by diamondback moth, Plutella xylostella. CONSTITUTION: The synthetic insecticidal toxin gene for the transformation of Brassicae pants cryIAc has the nucleotide sequence of SEQ ID NO: 25. The synthetic cryIAc gene is prepared by the steps of: preparing a table showing the codon usage of cryIAc gene and Brassicae gene; preparing the revision strategy of cryIAc gene to have the nucleotide sequence similar to that of the Brassicae gene without change of the amino acid sequence; preparing 56 primers and carrying out the recursive PCR using 56 primers; and cloning the synthetic cryIAc gene and sequencing the synthetic cryIAc gene, in which the 56 primers are divided into three sets wherein the first primer set is from 1,344 to 1,854 base of the cryIAc gene, the second primer set is from 864 to 1,351 base of the cryIAc gene, and the third primer set is from 1 to 900 of the cryIAc gene.
摘要翻译：目的：提供一种用于转化芸苔属植物的合成杀虫毒素基因，从而有效地防止小菜蛾Plutella xylostella损害植物。构成：用于转化芸苔属植物cryIAc的合成杀虫毒素基因具有SEQ ID NO：25的核苷酸序列。通过以下步骤制备合成cryIAc基因：制备显示cryIAc基因和芸苔属基因的密码子使用的表; 制备cryIAc基因修饰策略，使核苷酸序列与Brassicae基因序列相似，不改变氨基酸序列; 制备56个引物并用56个引物进行递归PCR; 并克隆合成的cryIAc基因并对合成的cryIAc基因进行测序，其中56个引物被分成三组，其中第一个引物组为cryIAc基因的1,344至1,854个碱基，第二个引物组为864至1,351个碱基 cryIAc基因，并且第三引物组为cryIAc基因的1至900。

13. 发明公开

KR1020020013003A 배추과 작물의 형질전환을 위한 합성 곤충 독소 유전자 有权
标题翻译：合成杀虫剂基因转化BRASSICA植物
公开(公告)号：KR1020020013003A
公开(公告)日：2002-02-20
申请号：KR1020000046327
申请日：2000-08-10
申请人： 대한민국(농촌진흥청장)
发明人： 박범석 , 조현석 , 진용문 , 김호일
IPC分类号： C12N15/33
CPC分类号： C12N15/8201 , C07K14/325
摘要： PURPOSE: A synthetic insecticidal toxin gene for the transformation of Brassicae plants is provided, thereby effectively preventing plants from being damaged by diamondback moth, Plutella xylostella. CONSTITUTION: The synthetic insecticidal toxin gene for the transformation of Brassicae pants cryIAc has the nucleotide sequence of SEQ ID NO: 25. The synthetic cryIAc gene is prepared by the steps of: preparing a table showing the codon usage of cryIAc gene and Brassicae gene; preparing the revision strategy of cryIAc gene to have the nucleotide sequence similar to that of the Brassicae gene without change of the amino acid sequence; preparing 56 primers and carrying out the recursive PCR using 56 primers; and cloning the synthetic cryIAc gene and sequencing the synthetic cryIAc gene, in which the 56 primers are divided into three sets wherein the first primer set is from 1,344 to 1,854 base of the cryIAc gene, the second primer set is from 864 to 1,351 base of the cryIAc gene, and the third primer set is from 1 to 900 of the cryIAc gene.
摘要翻译：目的：提供用于转化芸苔属植物的合成杀虫毒素基因，从而有效防止小菜蛾（Plutella xylostella）损害植物。构成：用于转化Brassicae裤子cryIAc的合成杀虫毒素基因具有SEQ ID NO：25的核苷酸序列。通过以下步骤制备合成cryIAc基因：制备显示cryIAc基因和Brassicae基因的密码子使用的表; 准备cryIAc基因的修订策略，使核苷酸序列与芸苔属基因的核苷酸序列相似，而不改变氨基酸序列; 制备56个引物，并使用56个引物进行递归PCR; 并克隆合成的cryIAc基因并对合成的cryIAc基因进行测序，其中将56个引物分成三组，其中第一引物组为cryIAc基因的1344至1854碱基，第二引物组为864至1351碱基 cryIAc基因和第三引物组是cryIAc基因的1至900个。

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