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11. 发明申请

US20100154083A1 Methods and Compositions for the Selection of A Transgenic Plant 审中-公开
标题翻译：转基因植物选择的方法和组成
公开(公告)号：US20100154083A1
公开(公告)日：2010-06-17
申请号：US12709630
申请日：2010-02-22
申请人： Margit C. Ross , Zuo-yu Zhao , Dennis L. Bidney , Drew L. Brumbaugh
发明人： Margit C. Ross , Zuo-yu Zhao , Dennis L. Bidney , Drew L. Brumbaugh
IPC分类号： A01H5/00 , C12Q1/68
CPC分类号： H02J7/0042 , F21S9/02 , F21V33/00 , F21W2131/1005
摘要： Compositions and methods are provided to screen, identify, select, isolate, and/or regenerate targeted integration events using seed priming. Seed priming provides the identification of a seed having stably incorporated into its genome a site-specific recombinase mediated integration of a selectable marker at a target locus operably linked to a promoter active in the seed.
摘要翻译：提供组合和方法以使用种子启动来筛选，识别，选择，分离和/或再生目标整合事件。种子引发提供了稳定地并入其基因组中的种子的鉴定，其位点特异性重组酶介导的可选择标记在可操作地连接到在种子中活化的启动子的靶基因座上的整合。

12. 发明申请

US20090263902A1 METHOD FOR REGULATING AGROBACTERIUM-MEDIATED TRANSFORMATION 有权
标题翻译：调节农杆菌介导转化的方法
公开(公告)号：US20090263902A1
公开(公告)日：2009-10-22
申请号：US12357284
申请日：2009-01-21
申请人： Luke MEHLO , Zuo-Yu Zhao
发明人： Luke MEHLO , Zuo-Yu Zhao
IPC分类号： C12N15/82 , C12N1/20
CPC分类号： C12N15/8205
摘要： A method for regulating expression of a virulence gene of Agrobacterium is described. The method comprises the steps of stimulating cereal cells, such as sorghum, so as to produce an active, typically phenolic, compound and exposing the Agrobacterium to this compound. The compound induces expression of the virulence gene of the Agrobacterium, effecting T-DNA transfer from the Agrobacterium to the cereal cells, which are thereby transformed.
摘要翻译：描述了调节农杆菌毒力基因表达的方法。该方法包括刺激谷类细胞如高粱的步骤，以产生活性的，通常为酚的化合物，并将土壤杆菌暴露于该化合物。该化合物诱导土壤杆菌的毒力基因的表达，从而转化从土壤杆菌到谷物细胞的T-DNA转移。

13. 发明申请

US20080299658A1 Highly Transformable Elite Doubled Haploid Line PHWVZ 审中-公开
标题翻译：高度可变的精英双倍倍数单倍体PHWVZ
公开(公告)号：US20080299658A1
公开(公告)日：2008-12-04
申请号：US11755083
申请日：2007-05-30
申请人： Zuo-Yu Zhao , Ning Wang
发明人： Zuo-Yu Zhao , Ning Wang
IPC分类号： A01H1/02 , A01H5/00 , C12N5/02 , C12N5/04
CPC分类号： A01H5/10
摘要： A novel double haploid maize line designated PHWVZ and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PHWVZ with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWVZ through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PHWVZ or a trait conversion of PHWVZ with another maize line. Inbred maize lines derived from double haploid maize line PHWVZ, methods for producing other inbred maize lines derived from double haploid maize line PHWVZ and the inbred maize lines and their parts derived by the use of those method.
摘要翻译：一种新型双倍体玉米品系，命名为PHWVZ及其种子，植物和植物部分。用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍体单倍体玉米品系PHWVZ。用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWVZ中的一种或多种性状，以及由此产生的玉米种子，植物和植物部分。杂交玉米种子，植物或植物部分通过双重单倍体系PHWVZ或PHWVZ与另一个玉米品系的转化而产生。来自双倍体单倍体玉米品系PHWVZ的近交玉米品系，由双倍体单倍体玉米品系PHWVZ和近交玉米品系及其部分产生的其他近交玉米品系的生产方法。

14. 发明申请

US20080131896A1 Methods of Transforming Plants and Identifying Parental Origin of a Chromosome In Those Plants 审中-公开
标题翻译：在这些植物中转化植物和识别染色体的亲本起源的方法
公开(公告)号：US20080131896A1
公开(公告)日：2008-06-05
申请号：US11941106
申请日：2007-11-16
申请人： Jerome P. Ranch , Wallace A. Marsh , Terry EuClaire Meyer , Dwight T. Tomes , Zuo-Yu Zhao , Gan-Yuan Zhong , Jimei Wang , Jeffery A. Mullen , Christopher J. Scelonge , Glenn S. Cole , John F. Soper
发明人： Jerome P. Ranch , Wallace A. Marsh , Terry EuClaire Meyer , Dwight T. Tomes , Zuo-Yu Zhao , Gan-Yuan Zhong , Jimei Wang , Jeffery A. Mullen , Christopher J. Scelonge , Glenn S. Cole , John F. Soper
IPC分类号： C12Q1/68
CPC分类号： C12N15/8202 , A01H1/04 , A01H5/10 , C12N15/8201 , C12N15/8205 , C12N15/8207 , C12N15/8209 , C12Q1/6855 , C12Q1/6895 , C12Q2600/156
摘要： Methods for plant transformation, for improving transformation efficiency, and for producing transgenic plants are provided. The methods comprise crossing a recipient plant from a genetic line of a plant species of interest with a donor plant selected from a transformation competent genetic line of the same plant species or of another closely related plant species to obtain a hybrid plant. Tissues obtained from the hybrid plant are transformation competent. These tissues can then be transformed with one or more nucleotide sequences of interest and selected for transgenic events having the nucleotide sequence of interest integrated within a chromosome derived from the recipient plant. Transformed cells can be selected and transgenic hybrid plants regenerated. The nucleotide sequence of interest can be introgressed into the genetic line from which the original recipient parent was derived, or into other genetic lines. Transformed plants and seeds are additionally provided.
摘要翻译：提供了用于植物转化，提高转化效率和用于产生转基因植物的方法。所述方法包括将受感染植物从感兴趣的植物物种的遗传系与选自相同植物物种的转基因遗传系或另一密切相关的植物物种的供体植物杂交以获得杂种植物。从混合植物获得的组织是转化能力。然后可以用一个或多个感兴趣的核苷酸序列转化这些组织，并选择用于整合在来自受体植物的染色体内的感兴趣的核苷酸序列的转基因事件。可以选择转化的细胞，并再生转基因杂交植物。感兴趣的核苷酸序列可以渗入原始受体亲本来源的基因系，或其他遗传系。另外提供转化的植物和种子。

15. 发明申请

US20060185033A1 Doubled haploid cells, embryos and plants 审中-公开
标题翻译：双倍体单倍体细胞，胚胎和植物
公开(公告)号：US20060185033A1
公开(公告)日：2006-08-17
申请号：US11298973
申请日：2005-12-09
申请人： Zuo-yu Zhao , Dennis Bidney , Evan Elsing , Michael Miller , Xinli Wu , William Gordon-Kamm
发明人： Zuo-yu Zhao , Dennis Bidney , Evan Elsing , Michael Miller , Xinli Wu , William Gordon-Kamm
IPC分类号： A01H1/00 , A01H5/00 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8241 , A01H5/10 , C12N15/8201 , C12N15/829
摘要： Methods for producing homozygous plants, seeds, and plant cells are provided. Methods of forming haploid tissue and then doubling the chromosomes to form doubled haploid cells are provided. Also provided are methods of transformation.
摘要翻译：提供了用于产生纯合植物，种子和植物细胞的方法。提供了形成单倍体组织，然后使染色体加倍以形成双倍体单倍体细胞的方法。还提供了转化的方法。

16. 发明授权

US06369298B1 Agrobacterium mediated transformation of sorghum 失效
标题翻译：农杆菌介导的高粱转化
公开(公告)号：US06369298B1
公开(公告)日：2002-04-09
申请号：US09056418
申请日：1998-04-07
申请人： Tishu Cai , Dorothy A. Pierce , Laura A. Tagliani , Zuo-Yu Zhao
发明人： Tishu Cai , Dorothy A. Pierce , Laura A. Tagliani , Zuo-Yu Zhao
IPC分类号： A01H100
CPC分类号： C12N15/8205
摘要： Methods and compositions for the efficient transformation of sorghum is provided. The method involves infection with Agrobacterium, particularly those comprising a super-binary vector. In this manner, any gene of interest can be introduced into the sorghum plant. The transformed gene will be flanked by at least one T-DNA border and present in the transformed sorghum in low copy number. Transformed sorghum, cells, tissues, plants, and seed are also provided. The invention encompasses regenerated, fertile sorghum plants, transgenic seeds produced therefrom, T1 and subsequent generations.
摘要翻译：提供了高效转化高粱的方法和组成。该方法涉及用农杆菌感染，特别是包含超二元载体的那些。以这种方式，可以将任何感兴趣的基因引入高粱植物中。转化的基因将侧接至少一个T-DNA边界并以低拷贝数存在于转化的高粱中。还提供了转化的高粱，细胞，组织，植物和种子。本发明包括再生的，可育的高粱植物，由其产生的转基因种子，T1和后代。

17. 发明授权

US06175058B1 Nucleic acid sequence encoding FLP recombinase 有权
标题翻译：编码FLP重组酶的核酸序列
公开(公告)号：US06175058B1
公开(公告)日：2001-01-16
申请号：US09263128
申请日：1999-03-05
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
IPC分类号： A01H100
CPC分类号： C12N9/00 , C12N15/8213 , C12N15/8289
摘要： A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
摘要翻译：在单子叶植物中，特别是在玉米中有效表达FLP重组酶的核酸序列。含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。

18. 发明授权

US5929301A Nucleic acid sequence encoding FLP recombinase 失效
标题翻译：编码FLP重组酶的核酸序列
公开(公告)号：US5929301A
公开(公告)日：1999-07-27
申请号：US972258
申请日：1997-11-18
申请人： Christopher L. Baszcynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao
发明人： Christopher L. Baszcynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao
IPC分类号： C12N9/00 , C12N15/82 , C12N5/04 , A01H5/00 , A01H5/10 , C12N15/29
CPC分类号： C12N9/00 , C12N15/8213 , C12N15/8289
摘要： A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
摘要翻译：在单子叶植物中，特别是在玉米中有效表达FLP重组酶的核酸序列。含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。

19. 发明授权

US08389807B2 Method for increasing efficiency of germplasm screening in plant transformation 失效
标题翻译：提高植物转化种质筛选效率的方法
公开(公告)号：US08389807B2
公开(公告)日：2013-03-05
申请号：US12649673
申请日：2009-12-30
申请人： Zuo-Yu Zhao , Xinli E. Wu , Myoeng-Je Cho , Deping Xu
发明人： Zuo-Yu Zhao , Xinli E. Wu , Myoeng-Je Cho , Deping Xu
IPC分类号： C12N15/84 , A01H4/00 , A01H1/04
CPC分类号： C12N15/8209 , A01H1/04 , C12N15/8201 , C12N15/8205
摘要： A method for increasing efficiency of germplasm screening for transformability may include providing a plurality of lines of plant target tissue to be transformed, characterizing each of the lines to provide characterization data, the characterization data comprises DNA or nucleic acid delivery technique response data and tissue culture response data, eliminating one or more of the plurality of lines based on the characterization data without performing transformation of the plurality of lines, such that a subset of the plurality of lines remains, and performing transformation experiments on the subset of the plurality of lines. The method may also include selecting a DNA or nucleic acid delivery technique protocol and a tissue culture protocol prior to the characterization.
摘要翻译：提高种质筛选效率的方法可包括提供要转化的多条植物靶组织，表征各条线以提供特征数据，表征数据包括DNA或核酸递送技术应答数据和组织培养响应数据，基于所述表征数据消除所述多行中的一个或多个，而不执行所述多条线的变换，使得保留所述多条线的子集，并对所述多条线的所述子集执行变换实验。该方法还可以包括在表征之前选择DNA或核酸递送技术方案和组织培养方案。

20. 发明授权

US08030560B2 Highly transformable elite doubled haploid line PHWVZ 有权
标题翻译：高度可变的精英双倍体单倍体PHWVZ
公开(公告)号：US08030560B2
公开(公告)日：2011-10-04
申请号：US12509538
申请日：2009-07-27
申请人： Zuo-Yu Zhao , Ning Wang
发明人： Zuo-Yu Zhao , Ning Wang
IPC分类号： A01H5/00 , A01H5/10 , A01H1/02 , C12N5/04
CPC分类号： A01H5/10
摘要： A novel double haploid maize line designated PHWVZ and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PHWVZ with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWVZ through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PHWVZ or a trait conversion of PHWVZ with another maize line. Inbred maize lines derived from double haploid maize line PHWVZ, methods for producing other inbred maize lines derived from double haploid maize line PHWVZ and the inbred maize lines and their parts derived by the use of those method.
摘要翻译：一种新型双倍体玉米品系，命名为PHWVZ及其种子，植物和植物部分。用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍体单倍体玉米品系PHWVZ。用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWVZ中的一种或多种性状，以及由此产生的玉米种子，植物和植物部分。杂交玉米种子，植物或植物部分通过双重单倍体系PHWVZ或PHWVZ与另一个玉米品系的转化而产生。来自双倍体单倍体玉米品系PHWVZ的近交玉米品系，由双倍体单倍体玉米品系PHWVZ和近交玉米品系及其部分产生的其他近交玉米品系的生产方法。

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