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11. 发明公开

EP0864860A1 Sample plate and multicapillary electrophoresis apparatus 失效
标题翻译：样品板和多毛细管电泳仪
公开(公告)号：EP0864860A1
公开(公告)日：1998-09-16
申请号：EP98104051.2
申请日：1998-03-06
申请人： Japan Science and Technology Corporation , THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH , SHIMADZU CORPORATION
发明人： Hayashizaki, Yoshihide , Nakamura, Shin, Shimadzu Corporation
IPC分类号： G01N27/447
CPC分类号： G01N27/44782 , G01N27/44743 , Y10S436/809
摘要： A base plate (101) is made of an insulating material, and comprised of a flat surface and a connector part (106) connected therewith. A plurality of wells (102) are vertically and transversely arranged on the surface of the base plate (101) at regular intervals respectively, and the respective wells (102) are provided with individual electrode patterns (104) reaching the connector part (106) from bottoms thereof through the surface of the base plate (101). The connector part (106) is connected to an external high-voltage application part.
摘要翻译：基板（101）由绝缘材料制成，并且由平坦表面和与其连接的连接器部分（106）构成。多个井（102）分别以规则的间隔垂直和横向地布置在基板（101）的表面上，并且各个井（102）设置有到达连接器部分（106）的单独的电极图案（104）从其底部穿过基板（101）的表面。连接器部分（106）连接到外部高电压施加部分。

12. 发明公开

EP0854362A2 Multi-capillary electrophoresis apparatus 失效
标题翻译： Mehrkapillarelektroforesevorrichtung
公开(公告)号：EP0854362A2
公开(公告)日：1998-07-22
申请号：EP98100389.0
申请日：1998-01-12
申请人： Japan Science and Technology Corporation , THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH , SHIMADZU CORPORATION
发明人： Hayashizaki, Yoshihide , Nakamura, Shin , Kashiwagi, Katsuya
IPC分类号： G01N27/447
CPC分类号： G01N27/44782 , G01N27/44721
摘要： After a sample stage (68) moves and brings a sample titer plate (66) toward and into contact with a capillary array end (2a), a high voltage is applied across electrodes (54, 58) for a prescribed time for injecting samples. Thereafter a electrophoresis reservoir (62) comes into contact with the capillary array end (2a), for starting electrophoresis. When electrophoresed/separated DNA fragments pass through a detected part (2c), an excitation/photoreceiving optical system (10) is so scanned that photomultipliers (30) detect fluorescence from four types of fluorescent materials labeling the samples. The excitation/photoreceiving optical system (10) comprises an epi-optical system (18) and a confocal optical system (22, 24), and is scanned at a high speed.
摘要翻译：在样品台（68）移动并使样品滴定板（66）朝向毛细管阵列端（2a）接触并接触毛细管阵列端（2a）之后，在电极（54,58）上施加高电压达预定时间以注射样品。此后，电泳槽（62）与毛细管阵列端（2a）接触，用于开始电泳。当电泳/分离的DNA片段通过检测部分（2c）时，激发/光接收光学系统（10）被扫描，光电倍增管（30）检测标记样品的四种类型的荧光材料的荧光。激发/光接收光学系统（10）包括外延光学系统（18）和共焦光学系统（22,24），并且被高速扫描。

13. 发明公开

EP0844048A2 Free form machining tool 失效
标题翻译： Freiform-Werkzeugmaschine
公开(公告)号：EP0844048A2
公开(公告)日：1998-05-27
申请号：EP97120762.6
申请日：1997-11-26
申请人： THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH , Ikegami Mold Engineering Co., Ltd.
发明人： Ohmori, Hitoshi
IPC分类号： B24B13/01 , B24B53/07 , B24D7/10
CPC分类号： B24B53/07 , B24B13/01 , B24D7/10
摘要： In a free form machining tool 10 for machining a surface to be machined while bringing the lower end into contact with the surface to be machined by a rotation around an axis z, the tool comprises a spherical tool 12 having a spherical surface machining section 13 on the lower portion, and a bearing 14 for supporting the spherical tool by an axis a of rotation which passes through the center O of the spherical surface and is different from the axis. The tool also includes rotating means 16 (for example, a turbine) for rotating the spherical tool around the axis of rotation. Thus, even if the spherical surface machining section comes into contact with the surface to be machined on the axis (radius: 0) of the tool, the spherical surface machining section is rotated around the axis a of rotation which is different from the axis z. Therefore, a peripheral speed of the axis is not zero (0). It is possible to efficiently work a freely curved surface with high accuracy and high quality by the use of a conventional three-axial NC machining apparatus.
摘要翻译：在用于加工待加工表面同时使下端通过围绕轴线z的旋转与待加工表面接触的自由形式加工工具10中，该工具包括球形工具12，球形工具12具有球面加工部分13 下部，以及用于通过旋转轴线支撑球形工具的轴承14，该轴线通过球面的中心O并且不同于轴线。该工具还包括用于围绕旋转轴线旋转球形工具的旋转装置16（例如，涡轮机）。因此，即使球面加工部与刀具的轴线（半径：0）接触，则球面加工部绕与轴线z不同的旋转轴线a旋转。因此，轴的圆周速度不为零（0）。通过使用传统的三轴NC加工装置，可以高精度，高品质地有效地进行自由曲面的加工。

14. 发明公开

EP0821059A3 Method for reverse transcription 失效
标题翻译：一种用于反转录方法
公开(公告)号：EP0821059A3
公开(公告)日：1998-04-22
申请号：EP97112672.7
申请日：1997-07-24
申请人： THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH
发明人： Hayashizaki, Yoshihide Tsukuba Life Science Center
IPC分类号： C12N15/10 , C07H21/02 , C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/6844 , C12Q2527/101 , C12Q2521/107 , C12Q2527/125
摘要： A method for preparing a cDNA from a mRNA using a reverse transcriptase wherein reverse transcription is performed at a temperature at which the mRNA does not take a secondary structure, for example, at a temperature of 45°C or more. The method is performed, for example, using a heat-labile reverse transcriptase in the presence of a substance exhibiting chaperone function having chaperone function such as saccharides. The method is performed, for example, in the presence of metal ions necessary for activation of the reverse transcriptase and a chelating agent for the metal ions such as a deoxynucleotide triphosphate. The method is capable of reverse transcription over the full length of mRNA template even if the mRNA is a long chain mRNA and, as a result, producing a full length cDNA.

15. 发明公开

EP0814156A3 Method for the purification of DNA 失效
标题翻译：一种用于DNA的纯化方法
公开(公告)号：EP0814156A3
公开(公告)日：1998-04-22
申请号：EP97109708.4
申请日：1997-06-14
申请人： THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH
发明人： Hayashizaki, Yoshihide c/o Inst.of Phys.and Chem.R
IPC分类号： C12N15/10 , C12Q1/68 , C07H1/08 , C12P19/34
CPC分类号： C12Q1/6806 , C12N15/101 , C12N15/1017
摘要： Disclosed is a method for collecting DNA by lysing microbial cells, adsorbing released DNA on a carrier and collecting the DNA adsorbed on the carrier, which method comprises the following steps of (1) lysing the microbial cells in the presence of the carrier so that the DNA obtained by lysing cells is adsorbed onto the carrier, separating solutions used for lysing cells and adsorbing DNA from the carrier, and eluting the DNA adsorbed on the carrier with a solution for eluting DNA and collecting eluted DNA, or (2) feeding microbial cells into a column comprising the carrier provided on a membrane filter capable of retaining a solution and permeating the solution when aspirated, lysing the microbial cells in the column so that the DNA obtained by lysing cells is adsorbed onto the carrier, separating solutions used for lysing cells and adsorbing DNA in the previous step from the column by aspiration, and feeding a solution for eluting DNA into the column and aspirating to collect the DNA adsorbed on the carrier. The methods of the present invention enable collection of DNA by the chaotropic ion method employing an apparatus with simpler structure and fewer operations.

16. 发明公开

EP0829765A1 Photosensitive resin composition 失效
标题翻译：光敏树脂组合物
公开(公告)号：EP0829765A1
公开(公告)日：1998-03-18
申请号：EP97115899.3
申请日：1997-09-12
申请人： THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH
发明人： Tajima, Yusuke , Ishii, Tadahiro , Takeuchi, Kazuo
IPC分类号： G03F7/038
CPC分类号： G03F7/038 , Y10S430/128
摘要： Disclosed are a photosensitive resin composition containing a fullerene and a polymer compound bearing a functional group which can react with the fullerene under irradiation of visible light; method for forming negative-type picture elements comprising steps of: forming a layer composed of the above composition, irradiating the layer with visible light through a photo mask having a pattern transmitting the visible light as a part of the mask to cure portions of the layer irradiated by the visible light, and removing uncured portions of the layer to form a resist film having cured portions of the pattern: and a method for producing devices comprising steps of: forming a layer of the above composition on an image-forming layer provided on a substrate, irradiating the layer with visible light through a photo mask having a pattern transmitting the visible light as a part of the mask to cure portions of the layer irradiated by the visible light, removing uncured portions of the layer to form a resist film having cured portions of the pattern, etching exposed portions of the image-forming layer, and removing the resist film. The composition and methods of the present invention enable production of negative type picture elements and devices with light of visible range.
摘要翻译：本发明公开了含有富勒烯和带有可在可见光照射下与富勒烯反应的官能团的聚合物化合物的光敏树脂组合物; 用于形成负型图像元件的方法包括以下步骤：形成由上述成分组成的层，通过具有透射可见光的图案的光掩模作为掩模的一部分用可见光照射该层，以固化该层的部分照射可见光并除去该层的未固化部分以形成具有该图案的固化部分的抗蚀剂膜的方法和用于制造器件的方法，该方法包括以下步骤：在图像形成层上形成上述组合物的层通过具有透射可见光的图案的光掩模作为掩模的一部分用可见光照射该层以固化由可见光照射的层的部分，去除该层的未固化部分以形成抗蚀剂膜，该抗蚀剂膜具有固化图案的部分，蚀刻图像形成层的曝光部分，并去除抗蚀剂膜。本发明的组合物和方法能够制造具有可见光范围的光的负型图像元件和器件。

17. 发明公开

EP0825265A1 Method for specifically amplifying a cDNA of an extremely small quantity 失效
标题翻译： Verfahren zur speziellen Amplifizierung von extrem geringen Mengen von cDNA
公开(公告)号：EP0825265A1
公开(公告)日：1998-02-25
申请号：EP97114019.9
申请日：1997-08-14
申请人： THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH
发明人： Sakamoto, Aiji , Hanaoka, Fumio
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/686
摘要： The invention provides a method for selectively amplifying a cDNA synthesized from an mRNA expressed only in an extremely small quantity. The method comprises synthesizing a cDNA from a target mRNA using an oligonucleotide primer complementary to at least a part of the 3' untranslated region of the mRNA and amplifying the resultant cDNA using a 5' primer and a 3' primer which is located 5' upstream of the oligonucleotide primer for synthesizing a cDNA.
摘要翻译：本发明提供了选择性扩增由极少量表达的mRNA合成的cDNA的方法。该方法包括使用与mRNA的3'非翻译区的至少一部分互补的寡核苷酸引物从靶mRNA合成cDNA，并使用位于5'上游的5'引物和3'引物扩增得到的cDNA 的用于合成cDNA的寡核苷酸引物。

18. 发明公开

EP0785278A1 METHOD OF DNA SEQUENCING 失效
标题翻译： VERFAHREN ZUR DNA-SEQUENZIERUNG
公开(公告)号：EP0785278A1
公开(公告)日：1997-07-23
申请号：EP95936097.5
申请日：1995-11-06
申请人： THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH
发明人： HAYASHIZAKI, Yoshihide The Institute of Physical , SASAKI, Nobuya The Institute of Physical
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6869 , C12Q2525/143 , C12Q2521/119
摘要： Disclosed is a method for determining a nucleotide sequence of DNA product amplified by polymerase chain reaction not requiring removal of primers and/or 2'-deoxyribonucleoside-5'-triphosphates and/or derivatives thereof, which comprises reacting ribonucleoside-5'-triphosphates comprising ATP, GTP, CTP, UTP and derivatives thereof and one or more of 3'-deoxyribonucleotide-5'-triphosphates comprising 3'-dATP, 3'-dGTP, 3'-dCTP, 3'-dUTP and derivatives thereof in the presence of an RNA polymerase and a DNA product which has been amplified by polymerase chain reaction and contains a promoter sequence for the RNA polymerase to afford a nucleic acid transcription product, separating the obtained nucleic acid transcription product and determining a nucleic acid sequence from the resluting separated fractions.
摘要翻译：公开了一种通过不需要除去引物和/或2'-脱氧核糖核苷-5'-三磷酸和/或其衍生物的聚合酶链反应扩增的DNA产物的核苷酸序列的方法，其包括使核糖核苷-5'-三磷酸 ATP，GTP，CTP，UTP及其衍生物和一种或多种包含3'-dATP，3'-dGTP，3'-dCTP，3'-dUTP及其衍生物的3'-脱氧核糖核苷酸-5'-三磷酸的RNA聚合酶和通过聚合酶链反应扩增的DNA产物，并含有用于RNA聚合酶的启动子序列以提供核酸转录产物，分离得到的核酸转录产物并从重新分离的分离出的核酸序列确定核酸序列分数。

19. 发明公开

EP0769892A1 Magnetic field adjusting center rods for cyclotron, magnet for cyclotron, and cyclotron 失效
标题翻译： ZentraleStäbezum Regeln des magnetischen Feldes eines Zyklotrons，MagnetfürZyklotron，und Zyklotron
公开(公告)号：EP0769892A1
公开(公告)日：1997-04-23
申请号：EP96116714.5
申请日：1996-10-17
申请人： THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH , Ion Kasokuki Kabushiki Kaisha
发明人： Nakanishi, Noriyoshi , Wakase, Shuichiro , Karasawa, Takashi
IPC分类号： H05H13/00 , H05H7/00
CPC分类号： H05H13/00 , H05H7/00
摘要： A pair of magnetic field adjusting center rods are inserted in central portions of a pair of pole pieces of a magnet included in a cyclotron, One of the magnetic field adjusting center rods is provided with an ion source receiving hole and the other is provided with a magnetic field adjusting recess coaxially with the ion source receiving hole. The magnetic field adjusting center rods are provided at their ends facing each other with magnetic field correcting projections, respectively. The magnetic field correcting projections project toward each other to form pole faces facing each other with a small air gap. Thus, irregularities in a magnetic field created between the pole pieces are corrected so that the dispersion of ion beams and the distortion of an orbit of ions can be prevented.
摘要翻译：一对磁场调节中心棒插入到包括在回旋加速器中的磁体的一对极片的中心部分中。一个磁场调节中心杆设置有离子源容纳孔，另一个设置有磁场调节凹槽与离子源接收孔同轴。磁场调节中心杆分别设置在磁场校正突起的彼此面对的端部。磁场校正突起朝向彼此突出以形成具有小气隙的彼此面对的极面。因此，校正在极片之间产生的磁场中的不规则性，从而可以防止离子束的分散和离子轨道的变形。

20. 发明公开

EP0737745A1 NOVEL SUGAR-CHAIN SYNTHETASE AND PROCESS FOR PRODUCING THE SAME 失效
标题翻译：神经合成酶FÜRZUCKERKETTEN UND VERFAHRENFÜRIHRE HERSTELLUNG
公开(公告)号：EP0737745A1
公开(公告)日：1996-10-16
申请号：EP95903933.0
申请日：1994-12-22
申请人： THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH
发明人： TSUJI, Shuichi The Institute of Physical and , KUROSAWA, Nobuyuki The Institute of Physical , HAMAMOTO, Toshiro The Institute of Physical , LEE, Young-Choon The Institute of Physical , NAKAOKA, Takashi The Institute of Physical , KOJIMA, Naoya The Institute of Physical
IPC分类号： C12N9/10 , C12N15/54
CPC分类号： C12N9/1081
摘要： Novel GalNAcα2,6-sialyltransferases P-B1 and P-B3; GalNAcα2,6-sialyltransferase genes encoding the above GalNAcα2,6-sialyltransferases P-B1 and P-B3; and an extracellularly releasable protein catalyzing GalNAcα2,6-sialic acid transfer which comprises a polypeptide portion as being an active domain of the GalNAcα2,6-sialyltransferase P-B1 or P-B3 together with a signal peptide are provided. Also provided is a process for preparing a sialyltransferases which enables efficient recovery of a sialyltransferase expressed in a large quantity in microorganisms.
摘要翻译：新型GalNAcα2,6-唾液酸转移酶P-B1和P-B3; 编码上述GalNAcα2,6-唾液酸转移酶P-B1和P-B3的GalNAcα2,6-唾液酸转移酶基因; 以及催化GalNAcα2,6-唾液酸转移的细胞外可释放蛋白，其包含多肽部分作为GalNAcα2,6-唾液酸转移酶P-B1或P-B3与信号肽的活性结构域。还提供了一种制备唾液酸转移酶的方法，其能够有效地回收在微生物中大量表达的唾液酸转移酶。

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