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11. 发明申请

US20070269096A1 METHODS FOR CLASSIFICATION OF SOMATIC EMBRYOS 有权
公开(公告)号：US20070269096A1
公开(公告)日：2007-11-22
申请号：US11833807
申请日：2007-08-03
申请人： Roger Timmis , Mitchell Toland , Timnit Ghermay , William Carlson , James Grob
发明人： Roger Timmis , Mitchell Toland , Timnit Ghermay , William Carlson , James Grob
IPC分类号： A01C1/00
CPC分类号： G06K9/00147 , A01C1/00 , A01H1/04 , A01H4/00 , B07C2501/009 , C12N15/8207
摘要： The present invention is directed towards methods for the classification of plant embryos by the application of one or more classification algorithms to analyze digitized images and absorption, transmittance, or reflectance spectra. The methods are generally applicable and emphasize the importance of acquiring and using as much image and absorption, transmittance, or reflectance spectral information as possible, based on objective criteria. The present invention allows automated selection of embryos most suitable for further culture and rejection of those seen as less suitable.

12. 发明授权

US5119588A Method and apparatus for culturing autotrophic plants from heterotrophic plant material 失效
标题翻译：用于培育来自异种植物材料的自植物的方法和装置
公开(公告)号：US5119588A
公开(公告)日：1992-06-09
申请号：US589052
申请日：1990-09-27
申请人： Roger Timmis , Mary E. Kreitinger , Michael J. Yancey
发明人： Roger Timmis , Mary E. Kreitinger , Michael J. Yancey
IPC分类号： A01H4/00 , C12N5/04
CPC分类号： C12N5/04 , A01H4/001
摘要： A method and apparatus is disclosed for growing embryonic or other undeveloped plant material in a controlled, biologically sterile environment to produce plants capable of surviving in soil in an uncontrolled environment. Cloned somatic embryos of a particular plant are individually embeddeed in separate sterile plugs comprised of a soil-like particulate medium. A volume of aqueous medium comprising a source of carbon and energy for the somatic embryos, and plant growth regulating substances, if required, is added to each plug. The sterile plugs are then isolated from the ambient environment while permitting exposure to light and atmospheric gases sufficient to effect development of photosynthesis in the developing embryos in the plugs. While in such isolation, the resulting plantlets develop roots and shoots. The resulting autotrophic plants are then removed from isolation and the growth medium is altered to remove the carbon and energy source to limit the capacity of the medium to support plant harming microbes. The plants are then transplanted. Several embodiments are disclosed involving compartmentalized trays and covering films or bags by which embryo-containing plugs are kept isolated from each other and from the environment during development of the embryos.
摘要翻译：公开了用于在受控的生物无菌环境中生长胚胎或其他未开发的植物材料以产生能够在不受控制的环境中在土壤中存活的植物的方法和装置。将特定植物的克隆的体细胞胚分别嵌入由包含土壤样颗粒介质的单独的无菌塞子中。如果需要，将一定体积的含有体细胞胚的碳源和能量的水性介质和植物生长调节物质加入到每个塞子中。然后将无菌塞从环境环境中分离，同时允许暴露于足以在插塞中发育的胚胎中发展光合作用的光和大气。在这种隔离下，所产生的小植株发育出根和芽。然后从分离中除去所得的自养植物，改变生长培养基以除去碳和能源，以限制培养基支持植物伤害微生物的能力。然后移植植物。公开了几个实施例，其涉及分隔的托盘和覆盖膜或袋，通过该薄膜或袋，含有胚胎的塞子在胚胎发育期间彼此隔离并且与环境保持隔离。

13. 发明申请

US20090007303A1 Methods for increasing germination vigor by early singulation of conifer somatic embryos 有权
标题翻译：通过早期分选针叶树体细胞胚增加萌发活力的方法
公开(公告)号：US20090007303A1
公开(公告)日：2009-01-01
申请号：US12164291
申请日：2008-06-30
申请人： Roger Timmis , James A. Grob , Pramod K. Gupta , Susan D. Rayfield
发明人： Roger Timmis , James A. Grob , Pramod K. Gupta , Susan D. Rayfield
IPC分类号： A01H5/00
CPC分类号： A01H1/04 , A01C1/02 , A01H3/00 , A01H3/04 , A01H4/001 , A01H4/008
摘要： In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) singulating a plurality of individual immature conifer somatic embryos from a first culture of embryos after a first incubation period in a first development media; and (b) contacting the plurality of singulated immature conifer somatic embryos with a second development media for a second incubation period.
摘要翻译：在一个方面，提供了一种用于增加在体外产生的针叶树体细胞胚的发芽势的方法。该方法包括：（a）在第一显影培养基中的第一次孵育期后，从第一胚胎培养物中分离多个单个未成熟针叶体细胞胚; 和（b）使多个单切的未成熟针叶树体细胞胚与第二培养基接触第二潜伏期。

14. 发明申请

US20080052056A1 METHODS FOR CLASSIFICATION OF SOMATIC EMBRYOS 审中-公开
标题翻译： SOMATIC EMBRYOS分类方法
公开(公告)号：US20080052056A1
公开(公告)日：2008-02-28
申请号：US11861213
申请日：2007-09-25
申请人： Roger Timmis , Mitchell Toland , Timnit Ghermay , William Carlson , James Grob
发明人： Roger Timmis , Mitchell Toland , Timnit Ghermay , William Carlson , James Grob
IPC分类号： G06G7/48
CPC分类号： A01H1/04 , A01C1/00 , A01C1/025 , A01H4/00 , G01N15/1475 , G06K9/00 , G06K9/00147
摘要： The present invention is directed towards methods for the classification of plant embryos by the application of one or more classification algorithms to analyze digitized images and absorption, transmittance, or reflectance spectra. The methods are generally applicable and emphasize the importance of acquiring and using as much image and absorption, transmittance, or reflectance spectral information as possible, based on objective criteria. The present invention allows automated selection of embryos most suitable for further culture and rejection of those seen as less suitable.
摘要翻译：本发明涉及通过应用一种或多种分类算法来分析数字化图像和吸收，透射率或反射光谱来分类植物胚胎的方法。这些方法通常是适用的，并强调基于客观标准获取和使用尽可能多的图像和吸收，透射率或反射光谱信息的重要性。本发明允许自动选择最适合进一步培养和排除那些被认为不太适合的胚胎。

15. 发明申请

US20070276640A1 METHODS FOR CLASSIFICATION OF SOMATIC EMBRYOS 审中-公开
标题翻译： SOMATIC EMBRYOS分类方法
公开(公告)号：US20070276640A1
公开(公告)日：2007-11-29
申请号：US11836095
申请日：2007-08-08
申请人： Roger Timmis , Mitchell Toland , Timnit Ghermay , William Carlson , James Grob
发明人： Roger Timmis , Mitchell Toland , Timnit Ghermay , William Carlson , James Grob
IPC分类号： A01H7/00 , G06G7/58
CPC分类号： G06K9/00147 , A01C1/00 , A01H1/04 , A01H4/00 , B07C2501/009 , C12N15/8207
摘要： The present invention is directed towards methods for the classification of plant embryos by the application of one or more classification algorithms to analyze digitized images and absorption, transmittance, or reflectance spectra. The methods are generally applicable and emphasize the importance of acquiring and using as much image and absorption, transmittance, or reflectance spectral information as possible, based on objective criteria. The present invention allows automated selection of embryos most suitable for further culture and rejection of those seen as less suitable.
摘要翻译：本发明涉及通过应用一种或多种分类算法来分析数字化图像和吸收，透射率或反射光谱来分类植物胚胎的方法。这些方法通常是适用的，并强调基于客观标准获取和使用尽可能多的图像和吸收，透射率或反射光谱信息的重要性。本发明允许自动选择最适合进一步培养和排除那些被认为不太适合的胚胎。

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