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11. 发明授权

US06306575B1 Methods for preparing enriched human hematopoietic cell preparations 失效
标题翻译：制备富集人造血细胞制剂的方法
公开(公告)号：US06306575B1
公开(公告)日：2001-10-23
申请号：US08566295
申请日：1995-12-01
申请人： Terry Thomas , Peter Lansdorp
发明人： Terry Thomas , Peter Lansdorp
IPC分类号： A01N102
CPC分类号： C12N5/0087 , A61K38/00 , C07K16/18 , C07K16/2803 , C07K16/2806 , C07K16/2809 , C07K16/283 , C07K16/289 , C07K16/2896 , C07K16/4283 , C12N5/0647 , C12N5/0693
摘要： The present invention relates to an antibody composition which contains antibodies specific for glycophorin A, CD3, CD24, CD16, CD14, and optionally CD45RA, CD38, CD36, CD38, CD56, CD2, CD19, CD66e, CD66b, and/or antibodies specific for antigens expressed on non-hematopoietic tumor cells. A process is also provided for enriching and recovering human hematopoietic progenitor cells and stem cells in a sample containing human hematopoietic differentiated, progenitor, and stem cells, and optionally tumor cells. The process involves reacting the sample with an antibody composition containing antibodies capable of binding to the antigens glycophorin A, CD3 CD24, CD16, and CD14, and optionally CD45RA, CD36, CD38, CD56, CD2, CD19, CD66e, CD66b, and/or antibodies specific for antigen expressed on non-hematopoietic tumor cells under conditions so that cell conjugates are formed between the antibodies and differentiated cells having the antigens glycophorin A, CD3 CD24, CD16, and CD14, and optionally CD45RA, CD38, CD36, CD38, CD56, CD2, CD19, CD66e, CD66b, and/or antibodies specific for antigens expressed on non-hematopoietic tumor cells on their surfaces. The cell conjugates are removed and a cell preparation is obtained which is enriched in human hematopoietic progenitor cells and stem cells. The invention also relates to kits for carrying out this process and to the cell preparations prepared by the process.
摘要翻译：本发明涉及一种抗体组合物，其含有对血型糖蛋白A，CD3，CD24，CD16，CD14以及任选的CD45RA，CD38，CD36，CD38，CD56，CD2，CD19，CD66e，CD66b和/ 在非造血肿瘤细胞上表达的抗原。还提供了一种方法，用于在含有人造血细胞分化，祖细胞和干细胞以及任选的肿瘤细胞的样品中富集和回收人造血祖细胞和干细胞。该方法包括使样品与含有能够结合抗原血型糖蛋白A，CD3 CD24，CD16和CD14以及任选的CD45RA，CD36，CD38，CD56，CD2，CD19，CD66e，CD66b和/或在非造血肿瘤细胞上表达的抗原特异抗体的抗体，使得在抗体和具有抗原血型糖蛋白A，CD3 CD24，CD16和CD14以及任选的CD45RA，CD38，CD36，CD38，CD56的抗体和分化细胞之间形成细胞缀合物，CD2，CD19，CD66e，CD66b和/或在其表面上在非造血肿瘤细胞上表达的抗原特异的抗体。去除细胞缀合物，并获得富含人造血祖细胞和干细胞的细胞制剂。本发明还涉及用于实施该方法的试剂盒和本方法制备的细胞制剂。

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