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    • 17. 发明授权
    • Detection and determination of solid phase-associated factors
    • 固相相关因素的检测和测定
    • US06632599B1
    • 2003-10-14
    • US09215209
    • 1998-12-18
    • Michael KrausCarsten SchelpWilhelm Schuy
    • Michael KrausCarsten SchelpWilhelm Schuy
    • C12Q100
    • G01N33/554G01N33/54313G01N2333/295Y10S435/81Y10S435/962Y10S435/968
    • The present invention relates to procedures for the detection or for the determination of solid phase-associated factors, which are multiply associated with the same solid phase. According to the invention, the sample is brought into contact with a transmitter particle, on which at least one ligand having binding affinity for a solid phase-associated factor and a transmitter are immobilized, and a receiver particle, on which at least one ligand having binding affinity for said solid phase-associated factor and a receiver is immobilized, and then the signal is determined which results when transmitter and receiver are brought sufficiently close to one another. In particular, the invention relates to the detection of cell surface receptors which can be used for the typing of cells or for the determination of cell activation states. It is thus possible to replace the hitherto widely customary flow cytometry by a more simple procedure.
    • 本发明涉及用于检测固相相关因子的测定方法,其与相同的固相相关联。 根据本发明,使样品与其上固定有至少一种与固相相关因子和发射体具有结合亲和力的配体的发射体颗粒和接收剂颗粒接触,其中至少一种配体具有 对所述固相关因子和接收器的结合亲和力被固定,然后确定当发送器和接收器彼此充分接近时导致的信号。 特别地,本发明涉及可用于细胞分型或确定细胞活化状态的细胞表面受体的检测。 因此,可以通过更简单的程序来替代迄今广泛使用的流式细胞术。