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11. 外观设计

USD927637S1 Water filter 有权
公开(公告)号：USD927637S1
公开(公告)日：2021-08-10
申请号：US29772567
申请日：2021-03-02
申请人： Jian Chen
设计人： Jian Chen

12. 发明申请

US20180049662A1 DIAGNOSTIC METHOD AND APPARATUS FOR BRAIN INJURY BASED ON EMG FREQUENCY POWER SPECTRA ANALYSIS 审中-公开
公开(公告)号：US20180049662A1
公开(公告)日：2018-02-22
申请号：US15526025
申请日：2015-12-29
申请人： Jian Chen
发明人： Jian Chen
IPC分类号： A61B5/0488 , A61B5/11 , A61B5/00
CPC分类号： A61B5/0488 , A61B5/112 , A61B5/4064 , A61B5/4076
摘要： A method and a device for diagnosing brain injury (e.g. concussion) based on analysis of electromyogram (EMG) signals and presence of neurological modulation impairment of motor activity are described. The method consists of recording specific EMG signals under defined conditions, processing the acquired EMG signals, extracting the relevant information, and making diagnosis of brain injury and disorders that are associated with brain pathology. Specifically, the steps and device involved in the method include placing an EMG electrode set on the subject's body area, acquiring EMG signals from a subject's muscle(s) undergoing contraction, processing the acquired EMG signals using a signal processing algorithm that includes Fourier transformation. The resulting EMG data with a frequency domain (frequency power spectra) are then analyzed in comparison with databases stored in the device and used to determine anomaly of diagnostic value in the EMG power spectra from subjects. A diagnosis can be made based the altered EMG frequency power spectra that reflect the neurological modulation impairment of motor neurons. The diagnostic value, determination, management suggestions are displayed on the device.

13. 发明申请

US20180042251A1 Mutant strain of Lactococcus lactis and its application 审中-公开
公开(公告)号：US20180042251A1
公开(公告)日：2018-02-15
申请号：US15640903
申请日：2017-07-03
申请人： Juan Zhang , Zhengming Zhu , Jian Chen , Guocheng Du , Peishan Yang
发明人： Juan Zhang , Zhengming Zhu , Jian Chen , Guocheng Du , Peishan Yang
IPC分类号： A23C9/123 , A23L19/20 , C12R1/46
CPC分类号： A23C9/123 , A23B7/105 , A23B7/155 , A23L19/20 , A23V2002/00 , A23Y2240/41 , C12R1/46
摘要： The present invention relates to a mutant strain of Lactococcus lactis and its application, and belongs to the field of food biotechnology. The mutant strain, Lactococcus lactis WH101, was screened for its better tolerance to harsh environmental factors, especially higher acid tolerance. The OD600 of Lactococcus lactis WH101 was increased by 5.5 times than that of the parent strain when cultured at pH 4.5. The survival rate of the mutant strain was 22.4 times higher than that of the parent strain after 3 hr treatment in pH 4.0 culture medium. The survival rate of the mutant strain was 5.2, 2.0 and 1.9 times over that of the parent strain treated with 15% ethanol for 4 hr, 15% NaCl for 6 hr and 1 mM H2O2 for 3 hr, respectively.

14. 发明授权

US09684718B2 System for searching for a web document 有权
公开(公告)号：US09684718B2
公开(公告)日：2017-06-20
申请号：US13560976
申请日：2012-07-27
申请人： Sheng Hua Bao , Jian Chen , Zhong Su , Rui Wang
发明人： Sheng Hua Bao , Jian Chen , Zhong Su , Rui Wang
IPC分类号： G06F17/30
CPC分类号： G06F17/30864
摘要： Provided is a system for searching for a web document. The system includes a receiver configured to receive a user's search request. A search engine is configured to search for a web document related to the search request based on a web document index, and to determine a move path for accessing the web document based on a move path index of the web document. A sender is configured to send the move path for accessing the web document to the user.

15. 发明授权

US09682381B2 Shredder feeder 有权
公开(公告)号：US09682381B2
公开(公告)日：2017-06-20
申请号：US14289325
申请日：2014-05-28
申请人： Licheng Zhou , Jian Chen , Jianbo Qi , Haiting Chen , Ziqiang Ding
发明人： Licheng Zhou , Jian Chen , Jianbo Qi , Haiting Chen , Ziqiang Ding
IPC分类号： B02C18/24 , B02C18/22 , B02C18/00 , B65H5/00 , B02C18/16
CPC分类号： B02C18/2275 , B02C18/0007 , B02C18/24 , B02C2018/164 , B02C2018/2208 , B65H5/00
摘要： A document shredder is disclosed including a paper receptacle for receiving a load of paper and a shredding assembly. A metering assembly is interposed between the paper receptacle and the shredding assembly and is configured to separate a portion of the load of paper from the load of paper and permit the portion of the load of paper to move into the shredding assembly. The metering assembly may include a stop plate secured thereto and defining a lifting portion on a perimeter portion thereof. The lifting portion is configured to engage a lower edge of the portion of the load of paper upon rotation of the stop plate and lift the paper over the stop plate. The lifting portion may include a flange, which may be a portion of a cone, formed on a perimeter portion of the wheel and occupying a sector of the perimeter of less than 60 degrees.

16. 发明申请

US20160319272A1 Method for Directed DNA Evolution using Combinatorial DNA Libraries 审中-公开
标题翻译：使用组合DNA文库进行定向DNA进化的方法
公开(公告)号：US20160319272A1
公开(公告)日：2016-11-03
申请号：US15140408
申请日：2016-04-27
申请人： Zhen Kang , Jian Chen , Guocheng Du , Peng Jin
发明人： Zhen Kang , Jian Chen , Guocheng Du , Peng Jin
IPC分类号： C12N15/10
CPC分类号： C12N15/1058 , C12N15/1031 , C12Q2521/101 , C12Q2521/501 , C12Q2531/113 , C12Q2533/101 , C12Q2563/179
摘要： The present invention provides a method of rapid directed DNA evolution based on single-stranded combinatorial DNA mutant library. The single- and double-stranded mutant library are constructed either separately or simultaneously using editing primers that contain mutated nucleotides and are targeted to different regions of the parent DNA sequence. The mutant library is then inserted into expression vectors and mutants with desired property are obtained by high throughput screening. Evolution of promoters, enzymes and metabolic pathways were successfully achieved using this method and mutants with excellent properties were obtained. The method of the present invention is simple, rapid, and efficient. It can be used for directed evolution of regulatory sequence such as promoters and ribosome binding sites, and is especially suitable for introducing diverse mutations into protein encoding genes, leading to rapid directed evolution of gene of interest.
摘要翻译：本发明提供了基于单链组合DNA突变体文库的快速定向DNA进化的方法。使用含有突变核苷酸的编辑引物单独或同时构建单链和双链突变体文库，并且靶向母DNA序列的不同区域。然后将突变型文库插入表达载体中，并通过高通量筛选获得具有所需性质的突变体。使用该方法成功实现了启动子，酶和代谢途径的进化，获得了具有优异性能的突变体。本发明的方法简单，快速，高效。它可以用于调节序列的定向进化，如启动子和核糖体结合位点，特别适用于将多种突变引入到蛋白质编码基因中，导致感兴趣的基因的快速定向进化。

17. 发明授权

US09382510B2 Methods and devices for electroporation 有权
标题翻译：电穿孔的方法和装置
公开(公告)号：US09382510B2
公开(公告)日：2016-07-05
申请号：US13594730
申请日：2012-08-24
申请人： Jian Chen
发明人： Jian Chen
IPC分类号： C12M1/42 , C12N13/00 , C12N15/87
CPC分类号： C12M35/02 , C12N13/00 , C12N15/87
摘要： An apparatus for electroporation of biological cells is provided. The apparatus includes a sample container having an insulator chamber for holding the cells. The sample container has a first electrode and a second electrode to provide electrical connection for electroporation. The insulator chamber is configured to contain at least one cell monolayer. The apparatus also includes a pulse generator that can generate a predetermined pulse for electroporation of the cells.
摘要翻译：提供了一种用于生物细胞电穿孔的装置。该装置包括具有用于保持电池的绝缘室的样品容器。样品容器具有第一电极和第二电极以提供用于电穿孔的电连接。绝缘体室配置成容纳至少一个细胞单层。该装置还包括脉冲发生器，其可以产生用于电穿孔的预定脉冲。

18. 发明申请

US20150176036A1 Genetically Engineered Torulopsis glabrata with Enhanced Extracellular Secretion of Pyruvic Acid 有权
标题翻译：基因工程的Torulopsis glabrata与丙酮酸的增强细胞外分泌
公开(公告)号：US20150176036A1
公开(公告)日：2015-06-25
申请号：US14185928
申请日：2014-02-21
申请人： Jian Chen , Hongwei Guo , Jingwen Zhou , Guocheng Du
发明人： Jian Chen , Hongwei Guo , Jingwen Zhou , Guocheng Du
IPC分类号： C12P7/40 , C12N15/81
CPC分类号： C12P7/40 , C07K14/195 , C12N1/16 , C12N15/815
摘要： The present invention provides a genetically engineered Torulopsis glabrata with enhanced extracellular secretion of pyruvic acid. T. glabrata strain was obtained from China Center for Type Culture Collection with CCTCC No: M202019 and over-expressed the optimized CutA (SEQ ID NO:2) encoding stress protein. Both of the temperature tolerance of T. glabrata and extracellular concentration of pyruvate were improved by overexpressing the optimized CutA. The optimum growth temperature of genetically engineered T. glabrata was increased too. The present invention can be widely used to increase extracellular levels of pyruvate during the fermentation process.
摘要翻译：本发明提供了具有增强的丙酮酸细胞外分泌的遗传工程化的Torulopsis glabrata。中华中心型号培养物中心获得T. glabrata菌株，CCTCC编号：M202019，过表达编码应激蛋白的优化的CutA（SEQ ID NO：2）。通过过度表达优化的CutA，提高了T. glabrata的温度耐受性和丙酮酸的细胞外浓度。转基因玉米的最佳生长温度也升高。本发明可广泛用于在发酵过程中增加丙酮酸的细胞外水平。

19. 发明申请

US20150079427A1 CASE SYSTEM, BATTERY AND BATTERY RACK WITH IMPROVED STACKING 审中-公开
标题翻译：案例系统，电池和电池架与改进的堆叠
公开(公告)号：US20150079427A1
公开(公告)日：2015-03-19
申请号：US14351039
申请日：2011-10-11
申请人： Jian Chen , De Li Wang
发明人： Jian Chen , De Li Wang
IPC分类号： H01M10/16 , H01M2/02 , H01M10/12 , H01M2/12
摘要： The present invention relates to a case system for lead batteries. The case system comprises of a one piece, essentially box shaped cell-housing part with at least 4 sidewalls enclosing an interior volume. At least one division plane divides said interior volume, such as to form a plurality of compartments. Each of the compartments is adapted for storing an electrical cell. The case system further comprises an access wall part with electrical conductor openings or terminal feed throughs and at least one fluid channel opening. Furthermore the case system comprises a closure wail part for sealing off said casing system. Each of the at least one division planes is essentially perpendicular to the same two opposite side walls of the essentially box shaped cell housing. The present invention further relates to a lead battery and a battery rack incorporating the above described advantageous case system.
摘要翻译：本发明涉及铅电池的壳体系统。壳体系统包括一个整体的，基本上为盒状的电池壳体部分，其中至少4个侧壁包围内部容积。至少一个分割平面将所述内部体积分开，以形成多个隔室。每个隔室适于存储电池。壳体系统还包括具有电导体开口或端子进料通道的入口壁部分和至少一个流体通道开口。此外，壳体系统包括用于密封所述壳体系统的封闭壁部分。所述至少一个分割平面中的每一个基本上垂直于基本上为箱状的单元壳体的相同的两个相对的侧壁。本发明还涉及一种包含上述有利案例系统的铅蓄电池和电池架。

20. 发明授权

US08902887B2 Load-balancing structure for packet switches and its constructing method 有权
标题翻译：分组交换机的负载均衡结构及其构建方法
公开(公告)号：US08902887B2
公开(公告)日：2014-12-02
申请号：US12739729
申请日：2009-10-31
申请人： Hui Li , Wei He , Shuoyan Li , Jiaqing Huang , Jian Chen , Kai Pan , Shuxin Zhang , Peng Yi , Binqiang Wang
发明人： Hui Li , Wei He , Shuoyan Li , Jiaqing Huang , Jian Chen , Kai Pan , Shuxin Zhang , Peng Yi , Binqiang Wang
IPC分类号： H04L12/28 , H04L12/939 , H04L12/933
CPC分类号： H04L49/101 , H04L49/552
摘要： This invention provides a load-balancing structure for packet switches and its constructing method. In this method, the structure based on self-routing concentrators is divided into two stages, that is, a first stage and a second stage fabric. A virtual output group queue (VOGQ) is appended to each input group port of the first stage fabric, and a reordering buffer (RB) is configured behind each output group port of the second stage fabric. Packets stored in the VOGQ are combined into data blocks with preset length, which is divided into data slices of fixed size, finally each data slice is added an address tag and is delivered to the first stage fabric for self-routing. Once reaching the RB, data slices are recombined into data blocks. This invention solves the packet out-of-sequence problem in the load-balancing Birkhoff-von Neumann switching structure and improves the end-to-end throughput.
摘要翻译：本发明提供了分组交换机的负载平衡结构及其构造方法。在这种方法中，基于自路由集中器的结构分为两个阶段，即第一阶段和第二阶段结构。将虚拟输出组队列（VOGQ）附加到第一级结构的每个输入组端口，并且在第二级结构的每个输出组端口之后配置重新排序缓冲器（RB）。存储在VOGQ中的数据包被组合成具有预设长度的数据块，其被划分为固定大小的数据片段，最后每个数据片段添加地址标签，并且被递送到第一级结构以进行自路由。一旦到达RB，数据切片被重新组合成数据块。本发明解决了负载平衡Birkhoff-von Neumann交换结构中的分组失序问题，提高了端到端吞吐量。

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