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11. 发明授权

US07867712B2 Nucleic acid sequences associated with cell states 有权
标题翻译：与细胞状态相关的核酸序列
公开(公告)号：US07867712B2
公开(公告)日：2011-01-11
申请号：US12063182
申请日：2006-08-08
申请人： James L. Sherley , Min-Soo Noh
发明人： James L. Sherley , Min-Soo Noh
IPC分类号： C12Q1/68 , G01N33/53 , C12P19/34
CPC分类号： C12Q1/6883 , C12Q2600/158
摘要： The present invention is directed to nucleic acid sequences whose expression is associated with different cell states, including nucleic acid sequences whose expression is induced at least 100-fold, or alternatively upregulated, in cells exhibiting asymmetric self-renewal relative to other cells. The invention is also directed to nucleic acid sequences whose expression is induced at least 100-fold, or alternatively upregulated, in cells exhibiting symmetric self-renewal relative to other cells.
摘要翻译：本发明涉及其表达与不同细胞状态相关的核酸序列，包括在其相对于其他细胞显示不对称自我更新的细胞中，其表达被诱导至少100倍或可选地上调的核酸序列。本发明还涉及其表达在相对于其他细胞显示对称自我更新的细胞中被诱导至少100倍或可选地上调的核酸序列。

12. 发明授权

US07655465B2 Methods for ex vivo propagation of somatic hair follicle stem cells 有权
标题翻译：体细胞毛囊干细胞体外增殖的方法
公开(公告)号：US07655465B2
公开(公告)日：2010-02-02
申请号：US11147013
申请日：2005-06-07
申请人： James L. Sherley , Johnathan King
发明人： James L. Sherley , Johnathan King
IPC分类号： C12N5/06
CPC分类号： C12N5/0628 , A61K35/12 , C12N2500/40
摘要： The present invention is directed to methods for readily propagating somatic hair follicle stem cells or melanocyte stem cells. The methods comprise enhancing guanine nucleotide (GNP) biosynthesis, thereby expanding guanine nucleotide pools. This in turn conditionally suppresses asymmetric cell kinetics in the explanted cells. The methods of the invention include pharmacological methods and genetic methods. For example, the resulting cultured somatic hair follicle stem cells can be used for a variety of applications including cell replacement therapies such as hair transplants, gene therapies, and tissue engineering applications, such as the generation of artificial skin and skin regeneration strategies including skin grafts.
摘要翻译：本发明涉及容易繁殖体细胞毛囊干细胞或黑素细胞干细胞的方法。该方法包括增强鸟嘌呤核苷酸（GNP）生物合成，从而扩大鸟嘌呤核苷酸库。这又有条件地抑制了移植细胞中的不对称细胞动力学。本发明的方法包括药理学方法和遗传方法。例如，所产生的培养的体细胞毛囊干细胞可用于各种应用，包括细胞替代疗法，例如毛发移植，基因治疗和组织工程应用，例如生成人造皮肤和皮肤再生策略，包括皮肤移植。

13. 发明授权

US5741646A Cell lines and methods for screening growth regulatory compounds 失效
标题翻译：用于筛选生长调节化合物的细胞系和方法
公开(公告)号：US5741646A
公开(公告)日：1998-04-21
申请号：US578207
申请日：1995-12-29
申请人： James L. Sherley , Lee B. Riley
发明人： James L. Sherley , Lee B. Riley
IPC分类号： G01N33/50 , C12Q1/68
CPC分类号： G01N33/5017
摘要： This invention provides a novel murine cultured cell line which is derived from primary mouse embryo fibroblasts and which exhibits inducer-regulated growth kinetics. The cell line has the potential to grow either linearly or exponentially depending on exposure to an appropriate inducing agent. A corresponding cell line that does not respond to the inducing agent is also provided. The paired cell line system provides a valuable research tool for the development of therapeutic agents that target cells exhibiting deregulated growth kinetics, and also enables the identification of potential carcinogenic agents that alter stem cell renewal kinetics.
摘要翻译：本发明提供了一种新型的鼠培养细胞系，其衍生自原代小鼠胚胎成纤维细胞，并且其表现出诱导剂调节的生长动力学。根据暴露于合适的诱导剂，细胞系具有线性或指数增长的潜力。还提供了对诱导剂不响应的相应细胞系。配对的细胞系系统为开发针对显示失调的生长动力学的细胞的治疗剂提供了有价值的研究工具，并且还能够鉴定改变干细胞更新动力学的潜在致癌剂。

14. 发明申请

US20130004965A1 DETECTING AND COUNTING TISSUE - SPECIFIC STEM CELLS AND USES THEREOF 有权
标题翻译：检测和计数组织 - 特异性干细胞及其用途
公开(公告)号：US20130004965A1
公开(公告)日：2013-01-03
申请号：US13513289
申请日：2010-12-03
申请人： James L. Sherley
发明人： James L. Sherley
IPC分类号： G01N33/566 , G01N33/573
CPC分类号： G01N33/56966 , G01N33/5073 , G01N33/5076
摘要： This invention provides methods of determining the number and percent of tissue specific stem cells (TSSCs) in a sample of cells, a population of cells or a sample of tissue. The methods rely on detecting the pattern-specific asymmetric localization of asymmetric self-renewal associated (ASRA) proteins or cell cycle specific proteins (CSSP) in cell undergoing asymmetrical self-renewal, which is a characteristic of TSSCs. The methods can be applied to any situations in which the percent of TSSC is desired such as laboratory research on adult stem cells, in drug development tests, prognostic indicator and therapeutic index, as a diagnostic and prognostic indicator and in monitoring TSSC expansion, e.g., in cell manufacturing processes.
摘要翻译：本发明提供了确定细胞样品，细胞群或组织样品中组织特异性干细胞（TSSC）的数量和百分比的方法。该方法依赖于检测不对称自我更新相关（ASRA）蛋白或细胞周期特异性蛋白（CSSP）在经历不对称自我更新的细胞中的模式特异性不对称定位，这是TSSC的特征。该方法可以应用于需要TSSC的百分比，如成人干细胞的实验室研究，药物开发测试，预后指标和治疗指标，作为诊断和预后指标以及监测TSSC扩张的任何情况，例如，在细胞制造过程中。

15. 发明授权

US07824912B2 Methods for ex vivo propagation of adult hepatic stem cells 有权
标题翻译：成体肝细胞离体繁殖方法
公开(公告)号：US07824912B2
公开(公告)日：2010-11-02
申请号：US11472238
申请日：2006-06-21
申请人： James L. Sherley , Krisha Panchalingam
发明人： James L. Sherley , Krisha Panchalingam
IPC分类号： C12N15/85 , A01K67/00
CPC分类号： C07K16/2857 , C12N5/0672 , C12N2501/11 , C12N2501/15 , C12N2503/02 , C12N2510/00 , G01N33/5073 , G01N2500/10
摘要： The present invention is directed to methods for readily propagating somatic liver stem cells. The methods comprise enhancing guanine nucleotide (GNP) biosynthesis, thereby expanding guanine nucleotide pools. This in turn conditionally suppresses asymmetric cell kinetics in the explanted cells. The methods of the invention include pharmacological methods and genetic methods. For example, the resulting cultured somatic liver stem cells can be used for a variety of applications including cell replacement therapies, gene therapies, drug discovery applications, and tissue engineering applications, such as the generation of artificial liver.
摘要翻译：本发明涉及容易繁殖体细胞肝干细胞的方法。该方法包括增强鸟嘌呤核苷酸（GNP）生物合成，从而扩大鸟嘌呤核苷酸库。这又有条件地抑制了移植细胞中的不对称细胞动力学。本发明的方法包括药理学方法和遗传方法。例如，所得的培养的体细胞肝干细胞可用于多种应用，包括细胞替代疗法，基因治疗，药物发现应用和组织工程应用，例如人造肝的产生。

16. 发明授权

US07645610B2 Hepatocyte precursor cell lines 有权
标题翻译：肝细胞前体细胞系
公开(公告)号：US07645610B2
公开(公告)日：2010-01-12
申请号：US10369428
申请日：2003-02-18
申请人： James L. Sherley , Hsuan-Shu Lee , Gracy G. Crane
发明人： James L. Sherley , Hsuan-Shu Lee , Gracy G. Crane
IPC分类号： C12N5/08
CPC分类号： C12N5/0672 , C12N2500/40
摘要： The present invention is directed to methods for readily generating hepatocyte precursor cell lines that retain hepatocyte-specific functions after extensive in vitro culturing. The methods comprise isolating and culturing hepatocyte precursor cell lines under permissive culture conditions that suppress asymmetric cell kinetics and allow exponential growth of the precursor cells, followed by transferring the hepatocyte precursor cell lines to non-permissive culture conditions that allow expression of asymmetric cell kinetics and induce expression of hepatocyte-specific characteristics.
摘要翻译：本发明涉及容易产生在广泛体外培养后保留肝细胞特异性功能的肝细胞前体细胞系的方法。所述方法包括在抑制不对称细胞动力学并允许前体细胞的指数生长的许可培养条件下分离和培养肝细胞前体细胞系，随后将肝细胞前体细胞系转移至允许表达不对称细胞动力学的非允许培养条件，诱导肝细胞特异性表达。

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