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11. 发明授权

EP0663447B1 Method of detecting a specific polynucleotide 失效
标题翻译：一种用于检测特异性多核苷酸的方法
公开(公告)号：EP0663447B1
公开(公告)日：2003-07-09
申请号：EP94309620.6
申请日：1994-12-21
申请人： EIKEN CHEMICAL CO., LTD. , TANABE SEIYAKU CO., LTD.
发明人： Hase, Tetsu, c/o EIKEN CHEMICAL CO., LTD. , Masubuchi, Harumi, c/o EIKEN CHEMICAL CO., LTD.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2565/301 , C12Q2521/319

12. 发明授权

EP0659880B1 Medium for culturing animal cells or antibody-producing cells 失效
标题翻译：生产培养基用于培养动物细胞或抗体的细胞
公开(公告)号：EP0659880B1
公开(公告)日：2001-02-21
申请号：EP94309730.3
申请日：1994-12-22
申请人： EIKEN CHEMICAL CO., LTD.
发明人： Hashimoto, Makoto, c/o EIKEN CHEMICAL CO., LTD. , Naito, Tsutomu, c/o EIKEN CHEMICAL CO., LTD.
IPC分类号： C12N5/00 , C12P21/00 , C12P21/08
CPC分类号： C12N5/163 , C12N5/0037 , C12N5/005 , C12N2500/30 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/90 , C12N2500/95 , C12N2501/999 , Y10S530/808 , Y10S530/809

13. 发明公开

EP2944959A1 Hemoglobin stabilizing storage solution 失效
标题翻译： ÄMOGLOBINSTABILISIERENDESPEICHERLÖSUNG
公开(公告)号：EP2944959A1
公开(公告)日：2015-11-18
申请号：EP15174423.2
申请日：2008-10-06
申请人： Eiken Chemical Co., Ltd.
发明人： SUGO, Shin
IPC分类号： G01N33/531 , G01N1/28 , G01N33/48 , G01N33/72 , C07C229/00
CPC分类号： G01N33/721 , C07C229/24 , G01N33/80 , Y10T436/108331
摘要： A hemoglobin stabilizing storage solution comprising hemoglobin and 3-hydroxy-2,2'-iminodisuccinic acid of Formula (2), or a salt thereof:

where X is a hydrogen atom, and alkali metal, or an ammonium group.
摘要翻译：包含式（2）的血红蛋白和3-羟基-2,2'-亚氨基二琥珀酸或其盐的血红蛋白稳定储存溶液：其中X为氢原子，碱金属或铵基。

14. 发明公开

EP0739638A1 METHOD OF STERILIZING PREFILLED SYRINGE MEDICINES 失效
标题翻译： VERFAHREN ZUR STERILISIERUNG VON MEDIKAMENTEN INVORGEFÜLLTENSPRITZEN
公开(公告)号：EP0739638A1
公开(公告)日：1996-10-30
申请号：EP95928621.2
申请日：1995-08-21
申请人： EIKEN CHEMICAL CO., LTD.
发明人： MATSUMOTO, Yasuyuki , SAITO, Masahiro , MORIKAWA, Junji , TAKANO, Hiroshi
IPC分类号： A61M5/31 , A61L2/06
CPC分类号： A61L2/06 , A61L2/26 , A61M5/001
摘要： The invention provides a method of sterilizing a chemical and an injector in a prefilled syringe and keeping them in sterilized condition, said method comprising mounting a liquid leakage preventive cap (1) on a tip end of an injector (2) in a prefilled syringe, filled with a chemical and mounting a sliding plug (3) in an opening at a rear end of the injector to prevent liquid leakage of the prefilled syringe, securing the prefilled syringe, which mounts thereto the liquid leakage preventive cap (1) and the sliding plug (3), by means of a securing container (6), sealing the securing container (6), to which the prefilled syringe (2) is fixed, by means of a poromeric and bacteria impermeable nonwoven paper or fabric to sterilize a chemical (5) in the prefilled syringe (2), the prefilled syringe (2) and the securing container at the same time. The securing container (6) is enclosed by a bag-shaped nonwoven paper or fabric (7). Alternatively, the securing container (6) may have an opening, and the opening may be sealed by the nonwoven paper or fabric (11). According to the invention, it is possible to manufacture a prefilled syringe kit readily at low cost in a sterilized manner and keep the sterilized condition.
摘要翻译：本发明提供了一种在预充式注射器中对化学药剂和注射器进行灭菌并将其保持在灭菌状态的方法，所述方法包括将防漏液帽（1）安装在预充式注射器中的注射器（2）的末端上，填充有化学品并将滑动塞（3）安装在喷射器后端的开口中，以防止预填充的注射器的液体泄漏，固定预充式注射器，该注射器安装在防漏液盖（1）上并滑动通过固定容器（6）将塞子（3），借助于通孔和细菌不可渗透的非织造纸或织物将固定的预充式注射器（2）固定到其上的固定容器（6）进行灭菌（5）在预充式注射器（2）中，预充式注射器（2）和固定容器同时进行。固定容器（6）由袋状非织造纸或织物（7）包围。或者，固定容器（6）可以具有开口，并且开口可以被非织造纸或织物（11）密封。根据本发明，可以以无菌方式以低成本容易地制造预填充注射器套件并保持灭菌状态。

15. 发明公开

EP0639647A2 Assay for detecting nucleic acid sequence 失效
标题翻译：用于检测核酸序列的测定
公开(公告)号：EP0639647A2
公开(公告)日：1995-02-22
申请号：EP94110526.4
申请日：1994-07-06
申请人： TANABE SEIYAKU CO., LTD. , EIKEN CHEMICAL Co., Ltd.
发明人： Yamagata, Koichi , Umemura, Isao , Shibatani, Takeji
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6846 , C12Q2565/107 , C12Q2521/101
摘要： Assay for detecting nucleic acid sequence, which comprises the steps: [A] contacting (1) a target single-stranded nucleic acid, (2) a pair of nucleic acid primers having nucleotide sequence complementary to the target nucleic acid and its complementary chain respectively, (3) a fluorescent-labeled nucleic acid probe which has a nucleotide sequence designed so as to be hybridized to the target nucleic acid downstream (at the side of 3'-terminus) from where the nucleic acid primer is hybridized and has a modified 3'-end so that the nucleotide chain is not elongated by a nucleic acid polymerase, and (4) a nucleic acid polymerase having exonuclease activity specific to a double-stranded nucleic acid in the presence of substrates for the enzyme (four kinds of nucleoside triphosphate), and thereby elongating the said nucleic acid primer chain and simultaneously hydrolyzing only the fluorescent-labeled nucleic acid probe hybridized to the target nucleic acid; [B] denaturating the chain-elongated product of primer to a single-stranded form; [C] amplifying the target nucleic acid by repeating the steps A and B; and [D] determining the change in fluorescence polarization resulted by the hydrolysis of the probe. This method is effective to detect the target nucleic acid sequence more simply with higher sensitivity and higher reliability in comparison with known methods, and further has advantages that because of no carryover or contamination of the amplified nucleic acids into subsequent reactions, undesirable false positive result can be avoided.
摘要翻译：（A）接触（1）目标单链核酸，（2）具有分别与目标核酸及其互补链互补的核苷酸序列的核酸引物对，所述核酸序列分析包括以下步骤：[A] ，（3）荧光标记的核酸探针，其具有被设计成与靶核酸杂交的核酸序列，所述靶核酸与核酸引物杂交的下游（3'末端侧）并具有修饰 3'末端，以便核酸聚合酶不延长核苷酸链，和（4）在酶的底物存在下具有对双链核酸特异的外切核酸酶活性的核酸聚合酶（四种核苷由此延长所述核酸引物链，并且同时仅水解与靶核酸杂交的荧光标记的核酸探针; [B]将引物的伸长链产物变性为单链形式; [C]通过重复步骤A和B扩增靶核酸; 和[D]确定由探针水解引起的荧光偏振的变化。与已知方法相比，该方法更有效地检测目标核酸序列，具有更高的灵敏度和更高的可靠性，并且还具有以下优点：由于扩增的核酸没有携带或污染到后续反应中，所以不希望的假阳性结果可以应避免。

16. 发明公开

EP0663447A2 Method of detecting a specific polynucleotide 失效
标题翻译： Verfahrenfürden Nachweis spezifischer Polynukleotiden。
公开(公告)号：EP0663447A2
公开(公告)日：1995-07-19
申请号：EP94309620.6
申请日：1994-12-21
申请人： EIKEN CHEMICAL CO., LTD. , TANABE SEIYAKU CO., LTD.
发明人： Hase, Tetsu, c/o EIKEN CHEMICAL CO., LTD. , Masubsuchi, Harumi, c/o EIKEN CHEMICAL CO., LTD.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2565/301 , C12Q2521/319
摘要： The present invention relates to a method of detecting a polynucleotide, comprising hybridizing a polynucleotide of known nucleotide sequence with a nuclease-resistant oligonucleotide primer having a sequence complementary to a part of said polynucleotide, then adding at least one kind of deoxynucleoside triphosphate, DNA polymerase and nuclease thereto, synthesizing a complementary strand being a nucleotide species located adjacent to the 3'-terminal of said primer and complementary to said polynucleotide, followed by decomposition thereof, the synthesis and decomposition of said complementary strand being repeated one or more times, and detecting the resulting pyrophosphoric acid or deoxynucleoside monophosphate. The present invention also includes a detection kit used for said method of detecting a polynucleotide.
摘要翻译：本发明涉及检测多核苷酸的方法，包括将已知核苷酸序列的多核苷酸与具有与所述多核苷酸的一部分互补的序列的核酸酶抗性寡核苷酸引物杂交，然后加入至少一种脱氧核苷三磷酸，DNA聚合酶合成作为与所述引物的3'末端相邻并且与所述多核苷酸互补的核苷酸的互补链，然后分解，所述互补链的合成和分解重复一次或多次，以及检测所得焦磷酸或脱氧核苷单磷酸。本发明还包括用于检测多核苷酸的所述方法的检测试剂盒。

17. 发明公开

EP0639647A3 Assay for detecting nucleic acid sequence 失效
标题翻译：一种用于检测核酸序列的方法。
公开(公告)号：EP0639647A3
公开(公告)日：1995-07-12
申请号：EP94110526.4
申请日：1994-07-06
申请人： TANABE SEIYAKU CO., LTD. , EIKEN CHEMICAL Co., Ltd.
发明人： Yamagata, Koichi , Umemura, Isao , Shibatani, Takeji
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6846 , C12Q2565/107 , C12Q2521/101
摘要： Assay for detecting nucleic acid sequence, which comprises the steps: [A] contacting (1) a target single-stranded nucleic acid, (2) a pair of nucleic acid primers having nucleotide sequence complementary to the target nucleic acid and its complementary chain respectively, (3) a fluorescent-labeled nucleic acid probe which has a nucleotide sequence designed so as to be hybridized to the target nucleic acid downstream (at the side of 3'-terminus) from where the nucleic acid primer is hybridized and has a modified 3'-end so that the nucleotide chain is not elongated by a nucleic acid polymerase, and (4) a nucleic acid polymerase having exonuclease activity specific to a double-stranded nucleic acid in the presence of substrates for the enzyme (four kinds of nucleoside triphosphate), and thereby elongating the said nucleic acid primer chain and simultaneously hydrolyzing only the fluorescent-labeled nucleic acid probe hybridized to the target nucleic acid; [B] denaturating the chain-elongated product of primer to a single-stranded form; [C] amplifying the target nucleic acid by repeating the steps A and B; and [D] determining the change in fluorescence polarization resulted by the hydrolysis of the probe. This method is effective to detect the target nucleic acid sequence more simply with higher sensitivity and higher reliability in comparison with known methods, and further has advantages that because of no carryover or contamination of the amplified nucleic acids into subsequent reactions, undesirable false positive result can be avoided.

18. 发明公开

EP0659880A1 Medium for culturing animal cells or antibody-producing cells 失效
标题翻译：生产培养基用于培养动物细胞或该抗体的细胞。
公开(公告)号：EP0659880A1
公开(公告)日：1995-06-28
申请号：EP94309730.3
申请日：1994-12-22
申请人： EIKEN CHEMICAL CO., LTD.
发明人： Hashimoto, Makoto, c/o EIKEN CHEMICAL CO., LTD. , Naito, Tsutomu, c/o EIKEN CHEMICAL CO., LTD.
IPC分类号： C12N5/00 , C12P21/00 , C12P21/08
CPC分类号： C12N5/163 , C12N5/0037 , C12N5/005 , C12N2500/30 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/90 , C12N2500/95 , C12N2501/999 , Y10S530/808 , Y10S530/809
摘要： The present invention relates to a medium for culturing animal cells containing at least one component selected from the group of substances mentioned below; a method for culturing animal cells comprising adding to a medium for the cells as a cell growth promoting substance at least one component selected from the group mentioned below; a method for enhancing the antibody production of antibody-producing cells comprising adding to a medium for the cells at least one component selected from the group mentioned below; a composition for enhancing the antibody production of antibody-producing cells containing at least one antibody production enhancing agent selected from the group mentioned below; and a method for producing a physiologically active substance comprising culturing animal cells on a medium containing at least one cell growth promoting substance selected from the group mentioned below, and then harvesting the cells grown or the substances produced by the cells.
D-penicillamine or salts thereof
Acetoacetic acid or salts thereof
Biguanides
Vitamin K₅ or salts thereof
N-acetyl-L-glutamic acid or salts thereof
摘要翻译：本发明涉及一种用于培养含有选自以下举出的物质中选择的至少一种成分的动物细胞培养基; 用于培养动物细胞包括向为所述细胞从下面提及的组中选择的至少一种成分的细胞生长促销婷物质的介质的方法; 为了提高抗体产生抗体产生细胞包括向用于从下面提及的组中选择的至少一种组分的细胞的培养基的方法; 为了提高抗体产生含有至少一种抗体的产生增强从下面提及的组中选择的试剂的抗体产生细胞的组合物; 和用于产生生理活性物质包含含有从下面提及的组中选择的至少一种细胞生长促销婷物质的培养基上培养的动物细胞，然后收获生长的细胞或由所述细胞产生的物质的方法。 D-青霉胺或其乙酰乙酸或它们的盐双胍类维生素K5或其盐的N-乙酰基-L-谷氨酸或其盐的盐

19. 发明专利

JP2014200200A &bgr;−ラクタマーゼ検出方法有权
标题翻译： -LACTAMASE检测方法
公开(公告)号：JP2014200200A
公开(公告)日：2014-10-27
申请号：JP2013079267
申请日：2013-04-05
申请人：栄研化学株式会社 , Eiken Chemical Co Ltd
发明人： KAWAI TOMOAKI , KAWAGUCHI KOHEI , FUJISAKI MOMOKO
IPC分类号： C12Q1/34 , C12M1/34 , C12Q1/02 , G01N31/22
摘要：【課題】短時間に&bgr;−ラクタマーゼの基質特異性に基づく詳細な酵素分類の判定が可能な方法及びそれに用いる試薬を提供することを課題とする。【解決手段】工程(a)及び(b)を含むことを特徴とする微生物の&bgr;−ラクタマーゼ検出方法及び判定方法であって、工程(a)が、第一の薬剤;ペニシリン系、第二の薬剤;第一世代セフェム系、第三の薬剤;第三世代セフェム系及び/または第四世代セフェム系、第四の薬剤;モノバクタム系、第五の薬剤;セファマイシン系及び/またはオキサセフェム系、並びに第六の薬剤;カルバペネム系、からなる6系統の&bgr;−ラクタム薬の各々を検出対象微生物に接触させ、各薬剤の分解の有無をアシドメトリー法により判別する工程であり、工程(b)が、工程(a)における各薬剤の分解有無の判別結果に基づいて検出対象微生物の酵素分類の判定を行う工程である、&bgr;−ラクタマーゼの検出方法及び判定方法。【選択図】なし
摘要翻译：要解决的问题：提供一种能够基于β-内酰胺酶的底物特异性以及其试剂在短时间内对酶进行详细分类的方法。解决方案：用于检测和测定微生物的方法 - 内酰胺酶包括以下步骤：（a）使以下六种类别的β-内酰胺与待测试的微生物接触，以通过酸度测定法确定各种药物的分解的存在或不存在，其中六类＆ β-内酰胺是：1.青霉素，2.第一代头孢烯，3.第三代头孢烯和/或第四代头孢烯，4.单宁，5.头孢霉素和/或oxacephem; 6.碳青霉烯; （b）基于步骤（a）中获得的分解结果确定待测试的目标微生物的酶分类。

20. 发明专利

JP2014160088A Method for measuring equol in biological specimen by immunological method, kit for measuring the same and method for determining equol-producing ability of subject 审中-公开
标题翻译：用于通过免疫学方法测量生物样本中的等价物的方法，用于测量其的试剂盒和确定主体的产生能力的方法
公开(公告)号：JP2014160088A
公开(公告)日：2014-09-04
申请号：JP2014095421
申请日：2014-05-02
申请人： Eiken Chemical Co Ltd , 栄研化学株式会社 , Otsuka Pharmaceut Co Ltd , 大塚製薬株式会社
发明人： MINEKAWA TAKAYUKI , SHINTOME KUMIKO , ABE KATSUSHI , OKUMA HIROSHI , ANDO CHIEKO , ABIRU YASUHIRO
IPC分类号： G01N33/53 , G01N33/533 , G01N33/534 , G01N33/535 , G01N33/543 , G01N33/553
CPC分类号： G01N33/5308 , C07K16/44
摘要： PROBLEM TO BE SOLVED: To provide a method for measuring equol in a biological specimen by an immunological method, a kit for measuring the equol therein, and a method for determining an equol-producing ability of a subject.SOLUTION: The present invention provides: a measurement method that uses S-equol as at least one antigen to be selected from the group consisting of a standard antigen used for preparing a calibration curve and a labelled antigen competing with equol in a biological specimen; a kit that includes S-equol as at least one antigen to be selected from the group consisting of the standard antigen and the labelled antigen; and a determination method that includes a step of measuring equol in the biological specimen derived from a subject having soybean isoflavone ingested by an immunological method using the S-equol as at least one antigen selected from the group consisting of the standard antigen and the labelled antigen, and a step of determining an equol-producing ability of the subject on the basis of a measurement value of the equol obtained by the step.
摘要翻译：要解决的问题：提供一种通过免疫学方法测量生物样品中的雌马酚的方法，用于测量牛尿酚的试剂盒，以及测定受试者的雌马酚产生能力的方法。本发明提供：使用S-牛尿酚作为选自用于制备校准曲线的标准抗原和在生物样本中与雌马酚竞争的标记抗原的至少一种抗原的测定方法; 包含作为至少一种选自标准抗原和标记抗原的抗原的S-牛尿酚的试剂盒; 以及测定方法，其包括测定来自具有大豆异黄酮的受试者的雌马酚的步骤，所述大豆异黄酮通过免疫学方法使用S-牛尿酚作为选自标准抗原和标记抗原的至少一种抗原以及基于通过该步骤获得的雌马酚的测定值来测定受试者的雌马酚产生能力的步骤。

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