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11. 发明申请

WO2008027501A3 LACCASES FOR PULP BIO-BLEACHING 审中-公开
公开(公告)号：WO2008027501A3
公开(公告)日：2008-03-06
申请号：PCT/US2007/019124
申请日：2007-08-31
申请人： VERENIUM CORPORATION , KEROVUO, Janne, Samuli , HAREMZA, Sylke , KOCH, Oliver , HABICHER, Tilo , ROBERTSON, Dan , DESANTIS, Grace , MCCANN, Ryan , LUGINBUHL, Peter
发明人： KEROVUO, Janne, Samuli , HAREMZA, Sylke , KOCH, Oliver , HABICHER, Tilo , ROBERTSON, Dan , DESANTIS, Grace , MCCANN, Ryan , LUGINBUHL, Peter
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12Q1/00 , C12Q1/34 , C12Q1/68 , C12P21/06 , C12P21/04 , A61K38/44 , C07H21/04 , C07K1/00 , C07K16/00
摘要： Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins and other phenolic and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.

12. 发明申请

WO2008027501A2 LACCASES FOR PULP BIO-BLEACHING 审中-公开
标题翻译：纸浆生物漂白用的胶乳
公开(公告)号：WO2008027501A2
公开(公告)日：2008-03-06
申请号：PCT/US2007019124
申请日：2007-08-31
申请人： VERENIUM CORP , KEROVUO JANNE SAMULI , HAREMZA SYLKE , KOCH OLIVER , HABICHER TILO , ROBERTSON DAN , DESANTIS GRACE , MCCANN RYAN , LUGINBUHL PETER
发明人： KEROVUO JANNE SAMULI , HAREMZA SYLKE , KOCH OLIVER , HABICHER TILO , ROBERTSON DAN , DESANTIS GRACE , MCCANN RYAN , LUGINBUHL PETER
IPC分类号： C12N9/06
CPC分类号： C12N9/0061 , C12N15/09 , C12P5/00 , C12P7/00 , C12P19/00 , C12Y110/03002 , D21C5/005 , D21C9/005 , D21C9/1063
摘要： Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins and other phenolic and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.
摘要翻译：本文提供了分离的漆酶和编码它们的核酸。还提供了用于漆酶反应的介体。本文还提供了使用漆酶氧化木质素和其它酚类和芳族化合物的方法，例如用于在高温和pH条件下木浆的生物漂白和脱色以促进漂白化学品的使用的显着减少，以及用于纤维处理。

13. 发明申请

WO2006128590A1 HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
标题翻译：水解物，核酸编码方法及其使用方法
公开(公告)号：WO2006128590A1
公开(公告)日：2006-12-07
申请号：PCT/EP2006/004736
申请日：2006-05-18
申请人： DSM IP ASSETS B.V. , MINK, Daniel , KIERKELS, Joannes, Gerardus, Theodorus , DESANTIS, Grace , FARWELL, Robert , WHIPPLE, Lawrence , BURK, Mark
发明人： MINK, Daniel , KIERKELS, Joannes, Gerardus, Theodorus , DESANTIS, Grace , FARWELL, Robert , WHIPPLE, Lawrence , BURK, Mark
IPC分类号： C12P41/00
CPC分类号： C12P7/62 , C12N9/16 , C12P17/06 , C12P41/005
摘要： The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phospholipase A, B, C and/or D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.
摘要翻译：本发明提供水解酶，编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。一方面，本发明涉及具有水解酶活性的多肽，例如酯酶，酰基转移酶，脂肪酶，磷脂酶（例如，磷脂酶A，B，C和/或D活性，patatin活性，脂质酰基水解酶（LAH）活性）或蛋白酶活性，包括热稳定和耐热水解酶活性，以及编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。本发明的多肽和肽的水解酶活性包括酯酶活性，脂肪酶活性（脂质水解），酸解反应（用游离脂肪酸代替酯化脂肪酸），酯交换反应（甘油三酸酯之间的脂肪酸交换），酯合成，酯交换反应，磷脂酶活性和蛋白酶活性（肽键的水解）。本发明的多肽可用于各种药物，农业和工业环境中，包括制造化妆品和营养品。另一方面，本发明的多肽用于合成对映体纯的手性产物。

14. 发明申请

WO2003097810A3 NITRILASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 审中-公开
公开(公告)号：WO2003097810A3
公开(公告)日：2003-11-27
申请号：PCT/US2003/015712
申请日：2003-05-15
申请人： DIVERSA CORPORATION , DESANTIS, Grace , SHORT, Jay, M. , BURK, Mark , WONG, Kevin , FARWELL, Robert , CHATMAN, Kelly
发明人： DESANTIS, Grace , SHORT, Jay, M. , BURK, Mark , WONG, Kevin , FARWELL, Robert , CHATMAN, Kelly
IPC分类号： C12N9/00
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

15. 发明申请

WO2003000840A3 NITRILASES 审中-公开
公开(公告)号：WO2003000840A3
公开(公告)日：2003-01-03
申请号：PCT/US2002/015983
申请日：2002-05-15
申请人： DIVERSA CORPORATION , MADDEN, Darcy , DESANTIS, Grace , CHAPLIN, Jennifer, Ann , WEINER, David, Paul , MILAN, Aileen , CHI, Ellen , SHORT, Jay, M. , BURK, Mark
发明人： DESANTIS, Grace , CHAPLIN, Jennifer, Ann , WEINER, David, Paul , MILAN, Aileen , CHI, Ellen , SHORT, Jay, M. , BURK, Mark
IPC分类号： C12N9/00 , C12N9/78 , C12N1/20 , C12N15/00 , C07H21/02 , C12P1/00 , C12P7/00 , C12P7/62 , C07K1/00
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

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