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131. 发明授权

US5830696A Directed evolution of thermophilic enzymes 失效
标题翻译：嗜热酶的定向进化
公开(公告)号：US5830696A
公开(公告)日：1998-11-03
申请号：US760489
申请日：1996-12-05
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C07K17/00 , C12N5/04 , C12N9/00 , C12N9/16 , C12N9/24 , C12N9/38 , C12N15/00 , C12N15/10 , C12N15/70 , C12N15/74 , C12N15/85 , C12N15/87 , C12P21/02 , C12P21/06 , C12Q1/68 , C07K1/00
CPC分类号： C12N15/1058 , C12N15/102 , C12N15/1034 , C12N9/00 , C12N9/16 , C12N9/2405 , C12N9/2468 , C12N9/2471 , C12Y302/01021 , C12Y302/01023
摘要： Thermostable enzyme are subjected to mutagenesis to produce a thermophilic enzyme which is stable at thermophilic temperature and which has increased activities at least two-fold higher than the activity of the wild-type thermostable enzyme at lower temperatures, which are temperatures of 50.degree. C. or lower.
摘要翻译：热稳定酶进行诱变以产生在嗜热温度下稳定的嗜热酶，并且其活性比野生型热稳定酶在较低温度（其为50℃）下的活性提高至少两倍。或更低。

132. 发明授权

US5589392A Nucleic acid construct encoding a nuclear transport peptide operatively linked to an inducible promoter 失效
标题翻译：编码与可诱导启动子有效连接的核转运肽的核酸构建体
公开(公告)号：US5589392A
公开(公告)日：1996-12-31
申请号：US158718
申请日：1993-11-29
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： A01K67/027 , C12N15/79 , C12N15/09 , C07H21/02 , C07H21/04 , C12N15/63
CPC分类号： A01K67/0275 , C12N15/79 , A01K2217/05
摘要： A system for regulating expression of eukaryotic genes in cells is described. The system contains two recombinant DNA molecules, a first molecule that encodes a nucleus-targeted inducible repressor polypeptide, and a second molecule that encodes an operator-regulated reporter polypeptide. Transgenic animals containing the system, and methods for using the system are also described.
摘要翻译：描述了一种用于调节细胞中真核基因表达的系统。该系统含有两个重组DNA分子，第一个分子编码核靶向诱导型阻遏物多肽，第二个分子编码操纵子调节的报道多肽。还描述了含有该系统的转基因动物以及使用该系统的方法。

133. 发明授权

US5188957A Lambda packaging extract lacking beta-galactosidase activity 失效
标题翻译： Lambda包装提取物缺乏β-半乳糖苷酶活性
公开(公告)号：US5188957A
公开(公告)日：1993-02-23
申请号：US661396
申请日：1991-02-26
申请人： Jay M. Short , Patricia Kretz
发明人： Jay M. Short , Patricia Kretz
IPC分类号： C07K14/01 , C12N1/20 , C12N1/21
CPC分类号： C07K14/005 , C12N1/20 , C12N2795/10322
摘要： A beta-galactosidase-free bacteriophage lambda DNA packaging extract is described. The extract can be produced from beta-galactosidase deficient lambda lysogens. Bacteriophage lambda lysogens having the genotype lacZ are provided.
摘要翻译：描述了不含β-半乳糖苷酶的噬菌体λDNA包装提取物。提取物可以由β-半乳糖苷酶缺陷的λ溶解因子产生。提供了具有基因型lacZ的噬菌体λ溶解因子。

134. 发明授权

US5128256A DNA cloning vectors with in vivo excisable plasmids 失效
标题翻译：具有体内可切除质粒的DNA克隆载体
公开(公告)号：US5128256A
公开(公告)日：1992-07-07
申请号：US341261
申请日：1989-04-20
申请人： William Huse , Joseph A. Sorge , Jay M. Short
发明人： William Huse , Joseph A. Sorge , Jay M. Short
IPC分类号： C12N15/64 , C12N15/70
CPC分类号： C12N15/70 , C12N15/64
摘要： Vectors are described that circumvent traditional DNA cloning and subcloning procedures, and that contain a unique DNA cartridge that permits both cloning of DNA directly into DNA sequences present within the cartridge, and in vivo removal and circularization of the cartridge thereby yielding an autonomously replicating structure. Because the DNA cartridge can include a wide variety of functional DNA sequences, the cloned DNA can be subjected to a plethora of molecular biological procedures without having to remove the cloned DNA from the cartridge thereby obviating the need to perform additional subcloning techniques. A particularly useful example of this type of vector is bacteriophage lambda containing the DNA cartridge.
摘要翻译：描述了可以克服传统DNA克隆和亚克隆程序的载体，并且其含有独特的DNA柱，其允许将DNA直接克隆到存在于盒内的DNA序列中，并且在体内移除和环化圆筒，从而产生自主复制的结构。因为DNA盒可以包括多种功能性DNA序列，所以克隆的DNA可以经受大量的分子生物学过程，而不必从盒中除去克隆的DNA，从而避免进行附加亚克隆技术的需要。这种载体的特别有用的实例是含有DNA盒的噬菌体λ。

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