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121. 发明授权

US5393674A Constitutent layer harvesting from a centrifuged sample in a tube 失效
标题翻译：从管中离心的样品收获的组成层
公开(公告)号：US5393674A
公开(公告)日：1995-02-28
申请号：US025343
申请日：1993-03-08
申请人： Robert A. Levine , Stephen C. Wardlaw
发明人： Robert A. Levine , Stephen C. Wardlaw
IPC分类号： G01N1/10 , A61B5/15 , B01L3/14 , G01N1/14 , G01N1/36 , G01N33/15 , G01N33/48 , G01N33/49 , G01N1/18
CPC分类号： G01N33/491 , B01L3/50215 , Y10T436/25375 , Y10T436/2575
摘要： Constituent layers are harvested from a centrifuged multi-constituent material in an evacuated glass or clear plastic tube which contains a float. When possibly contaminated materials, such as blood, are being tested, the use of an evacuated tube allows the measurements to be made without the technician being exposed to the blood. The tubes are large enough to hold approximately one ml of blood, and are filled with an inert gas at low pressure. The floats are formed with a through bore into which cell bands to be harvested will settle during centrifugation. The cell bands are stabilized by a layer of a flowable material which settles onto the plasma layer during centrifugation and forms a pellicle thereon. The cell layers to be harvested are aspirated from the float bore by way of a hypodermic needle or cannula inserted into the tube and float bore.
摘要翻译：在真空玻璃或含有浮子的透明塑料管中从离心的多组分材料中收获成分层。当可能被污染的材料（例如血液）被测试时，使用真空管允许在没有技术人员暴露于血液的情况下进行测量。管足够大以容纳约1ml血液，并在低压下填充惰性气体。浮子形成有通孔，待收集的细胞带将在离心过程中沉降。细胞带通过可流动的材料层稳定，其在离心过程中沉降到等离子体层上并在其上形成防护薄膜组件。待收获的细胞层通过插入管中的皮下注射针或套管和浮子孔从浮子孔吸出。

122. 发明授权

US5360719A Determination of lymphocyte reactivity to specific 失效
标题翻译：测定淋巴细胞对特异性的反应性
公开(公告)号：US5360719A
公开(公告)日：1994-11-01
申请号：US954440
申请日：1992-09-30
申请人： Robert A. Levine , Stephen C. Wardlaw
发明人： Robert A. Levine , Stephen C. Wardlaw
IPC分类号： G01N33/50 , G01N33/53 , G01N33/569 , C12Q1/04 , G01N33/49
CPC分类号： G01N33/5091 , G01N33/56972 , Y10S435/975 , Y10T436/25375
摘要： A patient's blood sample is incubated with an antigen and tested for lymphocyte response, i.e. an activation of lymphocytes and/or a conversion of lymphocytes to lymphoblasts, which indicates prior exposure of the patient to the antigen. A positive response indicates the presence of prior exposure to prior diseases or clinical conditions such as parasitic diseases, tuberculosis, salmonellosis, gonorrhea, fungal infections, rickettsial infections, Lyme disease or allergens. Whole blood from the patient is incubated with the antigen of the disease or condition for which the patient is being tested. After a suitable time, a fluorescent dye or colorant which has an affinity for a discriminant characteristic of the activated lymphocytes or lymphoblasts, such as: intracellular calcium; surface activation antigens such as transferrin receptor; HLA-Dr; Leu-23; and the like. The incubated blood is then drawn into a transparent tube containing a float which concentrates the buffy coat constituent layers upon centrifugation of the blood sample. The concentrated lymphocyte layer is then examined for fluorescence or coloration which is indicative of the presence of the activated lymphocytes or lymphoblasts, and their concentration. The fluorescence or coloration can be qualified and/or quantified by a reader instrument.
摘要翻译：将患者的血液样品与抗原一起孵育并测试淋巴细胞反应，即淋巴细胞的激活和/或淋巴细胞向淋巴母细胞的转化，其指示患者事先暴露于抗原。阳性反应表明先前接触先前疾病或临床病症如寄生虫病，结核病，沙门氏菌病，淋病，真菌感染，立克次体感染，莱姆病或过敏原。将来自患者的全血与患者正在测试的疾病或病症的抗原一起温育。在合适的时间之后，对活化的淋巴细胞或淋巴母细胞的鉴别特征具有亲和力的荧光染料或着色剂，例如：细胞内钙; 表面活化抗原如转铁蛋白受体; HLA-Dr; Leu-23; 等等。然后将孵育的血液吸入含有浮子的透明管中，该浮子在血液样品离心时浓缩血沉棕黄层组成层。然后检查浓缩的淋巴细胞层的荧光或着色，其指示活化的淋巴细胞或淋巴母细胞的存在及其浓度。荧光或着色可由阅读器仪器进行鉴定和/或定量。

123. 发明授权

US5342790A Apparatus for indirect fluorescent assay of blood samples 失效
标题翻译：血液样品间接荧光检测仪器
公开(公告)号：US5342790A
公开(公告)日：1994-08-30
申请号：US969379
申请日：1992-10-30
申请人： Robert A. Levine , Stephen C. Wardlaw , Leon W. M. M. Terstappen , Thomas J. Mercolino , Diether J. Recktenwald
发明人： Robert A. Levine , Stephen C. Wardlaw , Leon W. M. M. Terstappen , Thomas J. Mercolino , Diether J. Recktenwald
IPC分类号： G01N33/543 , B01L3/14 , G01N33/49 , G01N33/537 , G01N33/569 , G01N33/58 , G01N33/536
CPC分类号： B01L3/5021 , G01N33/491 , G01N33/5375 , G01N33/56972 , G01N33/585 , Y10S435/81 , Y10S435/967 , Y10S435/971 , Y10S435/973 , Y10S436/805 , Y10S436/81 , Y10S436/824 , Y10S436/829 , Y10T436/111666
摘要： A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labeled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made. Unbound labeled antibodies will be washed away from the complexed layers by the washing action of the descending blood cells during the centrifugation step. Unbound labeled antibodies will thus not interfere with the analysis.
摘要翻译：通过在透明管中离心血液样品来诊断患者的健康，该管含有一组或多组颗粒，例如每组的不同密度的溶血细胞或塑料珠。每组密度定义的颗粒携带对可能在被测试的血液样品中的补体抗原或抗体特异性的抗原或抗体，并且其表示患者的健康。向血液样品中加入对所有结合的抗体/抗原对特异性的标记标签抗体，以在血液样品中形成标记抗体+抗原 - 抗体复合物（AAAC）。离心后，根据颗粒的相应密度，络合的颗粒将沉淀在管中的不同区域中，并且颗粒层的标签发射程度可以使得要进行血液样品的定性或定量分析。在离心步骤中，未结合的标记抗体将通过下降血细胞的洗涤作用从复合层中洗去。因此，未结合的标记抗体不会干扰分析。

124. 发明授权

US5251474A Centrifuged material layer measurement in an evacuated tube 失效
标题翻译：在真空管中离心材料层测量
公开(公告)号：US5251474A
公开(公告)日：1993-10-12
申请号：US822321
申请日：1992-01-16
申请人： Stephen C. Wardlaw , Robert A. Levine
发明人： Stephen C. Wardlaw , Robert A. Levine
IPC分类号： B01L3/14 , G01N33/48 , G01N33/49 , G01N33/50 , B01D17/038 , B01D21/26 , B04B5/04
CPC分类号： B01L3/50215 , G01N33/48 , G01N33/491 , G01N33/5002
摘要： Centrifuged material layer measurements are made in an evacuated glass or clear plastic tube which contains a float. When possibly contaminated materials, such as blood, are being tested the use of the evacuated tube allows the measurements to be made without the technician being exposed to the blood. The tubes are large enough to hold approximately one ml of blood, and are filled with an inert gas at low pressure. The floats are formed with a through passage into which the cell bands to be expanded will settle during centrifugation.
摘要翻译：离心材料层的测量在包含浮子的真空玻璃或透明塑料管中进行。当可能受污染的材料（例如血液）被测试时，使用真空管允许在没有技术人员暴露于血液的情况下进行测量。管足够大以容纳约1ml血液，并在低压下填充惰性气体。浮子形成有通孔，待离散的细胞带将在其中沉降。

125. 发明授权

US4940668A Method for increasing agglutination of groups of cells to produce improved cell layer interface in centrifuged blood sample 失效
标题翻译：增加细胞组凝集以在离心血液样品中产生改善的细胞层界面的方法
公开(公告)号：US4940668A
公开(公告)日：1990-07-10
申请号：US98370
申请日：1987-09-18
申请人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez , Michael R. Loken
发明人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez , Michael R. Loken
IPC分类号： G01N33/49 , G01N33/50 , G01N33/80
CPC分类号： G01N33/80 , G01N33/491 , G01N33/5002 , Y10T436/107497 , Y10T436/25 , Y10T436/25375
摘要： In order to produce a more well defined interface between adjacent cell layers in a centrifuged sample of anticoagulated whole blood, a material which will bond one group of cells together is added to the blood sample prior to centrifugation. The bonding material must produce a high strength bond between one group of cells, but not effect the other cell types. The material is added prior to centrifugation of the blood sample.
摘要翻译：为了在离心的抗凝全血样品中产生相邻细胞层之间更加界定的界面，将离心之前将一组细胞结合在一起的物质加入到血液样品中。接合材料必须在一组电池之间产生高强度的结合，但不影响其他电池类型。在血液样品离心之前加入材料。

126. 发明授权

US4843869A Method for measuring hemoglobin 失效
标题翻译：血红蛋白测定方法
公开(公告)号：US4843869A
公开(公告)日：1989-07-04
申请号：US170771
申请日：1988-03-21
申请人： Robert A. Levine , Stephen C. Wardlaw
发明人： Robert A. Levine , Stephen C. Wardlaw
IPC分类号： G01N33/48 , B01L3/14 , G01N9/12 , G01N33/49
CPC分类号： G01N9/12 , B01L3/50215 , G01N33/491
摘要： A whole blood sample is placed in a tube such as a capillary tube, with a plastic float. The float is axially elongated and has a specific gravity which causes it to float in the packed red blood cells when the sample is centrifuged in the tube. The hemoglobin concentration of the packed red blood cells is measured by measuring the depth that the float sinks into the red cell layer, and then the hemoglobin concentration of the blood is calculated. The hemoglobin can be measured in this manner because virtually the only red cell component which contributes density to the red cells is the hemoglobin. The hemoglobin concentration of the whole blood is calculated by multiplying the mean corpuscular hemoglobin concentration of the packed red blood cells by the packed cell volume % (hematocrit) of the whole blood. All of the necessary calculations may be performed by a preprogramed microprocessor.

127. 发明授权

US4779976A Multiparameter hematology measurement for veterinarians 失效
标题翻译：兽医多参数血液学测量
公开(公告)号：US4779976A
公开(公告)日：1988-10-25
申请号：US91380
申请日：1987-08-31
申请人： Robert A. Levine , Stephen C. Wardlaw
发明人： Robert A. Levine , Stephen C. Wardlaw
IPC分类号： G01N15/05 , G01N33/487 , G01N33/16
CPC分类号： G01N15/05
摘要： Blood samples of animals are taken and analyzed for several different cell parameters. The analysis is made by centrifuging an anticoagulated blood sample in a capillary tube containing a float which physically elongates certain of the component cell layers in the sample. The instrument in which the parameter measurements are made is computerized and can be preset to take measurements of the blood of any one of several different types of animals whose respective blood cells have different packing coefficients.
摘要翻译：拍摄动物的血液样品并分析几种不同的细胞参数。通过在含有漂浮物的毛细管中离心抗凝血液样品进行分析，物理地延长样品中的某些组分细胞层。进行参数测量的仪器是计算机化的，并且可以被预设为对各个血细胞具有不同包装系数的几种不同类型的动物中的任一种的血液进行测量。

128. 发明授权

US4695553A Method for increasing agglutination of groups of cells to produce improved cell layer interface in centrifuged blood sample using antibodies 失效
标题翻译：增加细胞组凝集的方法，以使用抗体在离心血液样品中产生改善的细胞层界面
公开(公告)号：US4695553A
公开(公告)日：1987-09-22
申请号：US794127
申请日：1985-11-01
申请人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez , Michael R. Loken
发明人： Stephen C. Wardlaw , Robert A. Levine , Rodolfo R. Rodriguez , Michael R. Loken
IPC分类号： G01N33/53 , C12Q1/00 , G01N33/48 , G01N33/49 , G01N33/50 , G01N33/80 , G01N1/18
CPC分类号： G01N33/5002 , G01N33/491 , G01N33/80 , Y10T436/25375
摘要： In order to produce a more well defined interface between adjacent cell layers in a centrifuged sample of anticoagulated whole blood, a material which will bond one group of cells together is added to the blood sample prior to centrifugation. The bonding material must produce a high strength bond between one group of cells, but not effect the other cell types. The material is added prior to centrifugation of the blood sample. An example of a bonding agent is a monoclonal antibody.
摘要翻译：为了在离心的抗凝全血样品中产生相邻细胞层之间更加界定的界面，将离心之前将一组细胞结合在一起的物质加入到血液样品中。接合材料必须在一组电池之间产生高强度的结合，但不影响其他电池类型。在血液样品离心之前加入材料。粘合剂的实例是单克隆抗体。

129. 发明授权

US4170995A Catheter clamp 失效
标题翻译：导管夹
公开(公告)号：US4170995A
公开(公告)日：1979-10-16
申请号：US853321
申请日：1977-11-21
申请人： Robert A. Levine , Stephen C. Wardlaw
发明人： Robert A. Levine , Stephen C. Wardlaw
IPC分类号： A61M25/02 , A61B17/00
CPC分类号： A61M25/02 , A61M2025/024 , Y10S128/26 , Y10T24/33 , Y10T24/3427
摘要： A holder for clamping in place a catheter or other hollow tube, such as a urinary catheter tube, a nasogastric tube or intravenous tube on a patient's body, which holder includes a pliant adhesive-bearing base which is adhered to the patient's skin. The holder is adjustable so as to be capable of holding the catheter tube against rotary and longitudinal movement, and also be capable of partially or completely closing the bore of the catheter tube by deforming the tube wall.
摘要翻译：用于将患者体内的导尿管或其它中空管例如导尿管，鼻胃管或静脉内管夹持的保持器，该保持器包括粘附到患者皮肤上的柔韧的粘合剂承载底座。保持器是可调节的，以便能够保持导管不受旋转和纵向移动，并且还能够通过使管壁变形来部分地或完全地封闭导管管的孔。

130. 发明授权

US4141654A Standard for calibrating and/or verifying instrument accuracy 失效
标题翻译：用于校准和/或验证仪器精度的标准
公开(公告)号：US4141654A
公开(公告)日：1979-02-27
申请号：US799178
申请日：1977-05-23
申请人： Stephen C. Wardlaw , Robert A. Levine
发明人： Stephen C. Wardlaw , Robert A. Levine
IPC分类号： B01L3/00 , B01L3/14 , G01N21/27 , G02B27/32 , G01C25/00
CPC分类号： B01L3/561 , B01L3/5021 , G02B27/32 , G01N21/278
摘要： A device for use in checking and/or calibrating the accuracy with which a blood constituent concentration measuring instrument is operating. The device includes a capillary tube size member having annular bands marked on its outer surface. The bands are of predetermined axial extent and simulate centrifuged blood cell or other constituent layers. The axial dimension of each band is measured on the instrument which provides a visual indication of corresponding blood cell count. The blood cell count indicated by the instrument is then compared to the known blood count corresponding to the predetermined axial extent of the particular band measured. This device may be used to calibrate the instrument or determine the accuracy with which the instrument is operating.
摘要翻译：用于检查和/或校准血液成分浓度测量仪器正在操作的精度的装置。该装置包括在其外表面上标记有环形带的毛细管尺寸构件。条带具有预定的轴向范围，并模拟离心血细胞或其他构成层。在仪器上测量每个带的轴向尺寸，其提供相应血细胞计数的视觉指示。然后将由仪器指示的血细胞计数与对应于所测量的特定带的预定轴向范围的已知血液计数进行比较。该设备可用于校准仪器或确定仪器运行的准确性。

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