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    • 116. 发明授权
    • Method for analysis of compound-binding ability of protein
    • 分析蛋白质化合物结合能力的方法
    • US08889423B2
    • 2014-11-18
    • US11989565
    • 2006-07-31
    • Yoshiya OdaHiroyuki Katayama
    • Yoshiya OdaHiroyuki Katayama
    • G01N30/02G01N24/00C07K1/22G01N30/84H01J49/04G01N33/68G01N30/72
    • G01N33/6848G01N30/72G01N30/84G01N33/54306G01N33/58G01N2030/027G01N2030/8411G01N2458/15G01N2560/00H01J49/0409Y10T436/24
    • A method for analyzing a binding ability of protein to a compound, comprising the steps of (a) fractionating a first group of isotope-labeled proteins into plural fractions using a carrier having the compound immobilized thereon; (b) fractionating a second group of proteins into one or plural fractions using a carrier having the compound immobilized thereon; (c) adding an amount of at least one fraction obtained in step (b) to each of the fractions obtained in step (a); (d) analyzing the fractions obtained in step (c) with mass spectrometry; and (e) based on the mass spectrometry information, obtaining, regarding each fraction, an intensity ratio between a peak derived from a protein in the fraction obtained in step (a) and a peak derived from a protein in the fraction obtained in step (b), and comparing degrees of the binding ability of the plural kinds of proteins to the compound.
    • 一种用于分析蛋白质与化合物的结合能力的方法,包括以下步骤:(a)使用其上固定有化合物的载体将第一组同位素标记的蛋白质分馏成多个级分; (b)使用其上固定化合物的载体将第二组蛋白质分馏成一个或多个级分; (c)将步骤(b)中获得的至少一个级分的量加入到步骤(a)中获得的每种级分中; (d)用质谱分析步骤(c)中得到的级分; 和(e)基于质谱信息,从每个级分获得在步骤(a)中获得的级分中衍生自蛋白质的峰与来自步骤(a)中获得的级分中的蛋白质的峰值之间的强度比, b),并且比较多种蛋白质与化合物的结合能力的程度。