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    • 103. 发明申请
    • MEANS AND METHODS FOR DETECTING METHYLATED DNA
    • 检测甲基化DNA的方法和方法
    • WO2006056480A2
    • 2006-06-01
    • PCT/EP2005/012707
    • 2005-11-28
    • KLINIKUM DER UNIVERSITÄT REGENSBURGREHLI, Michael
    • REHLI, Michael
    • C12Q1/6886C07K14/4703C07K2317/52C07K2319/00C07K2319/20C07K2319/30C07K2319/80C12Q2537/164
    • The present application relates to a nucleic acid molecule having a nucleotide sequence encoding a bifunctional polypeptide comprising the DNA-binding domain of a protein belonging to the family of Methyl-CpG binding proteins (MBDs) and the Fc portion of an antibody. In addition, vectors and host cells which comprise said nucleic acid molecule and polypeptides which are encoded by said nucleic acid molecule as well as processes for producing said polypeptide are disclosed. Moreover, the present application provides an antibody specifically binding said polypeptide and compositions, in particular diagnostic compositions comprising the nucleic acid molecule(s), vector(s), host cell(s), polypeptide(s) or antibodie(s) of the present application. Furthermore, methods and uses employing the polypeptides of the present invention for detecting methylated DNA, in particular in tumorous tissue or tumor cells are provided.
    • 本申请涉及具有编码双功能多肽的核苷酸序列的核酸分子,其包含属于甲基-CpG结合蛋白(MBD)家族的蛋白质和抗体的Fc部分的DNA结合结构域。 此外,公开了包含所述核酸分子和由所述核酸分子编码的多肽的载体和宿主细胞以及产生所述多肽的方法。 此外,本申请提供了特异性结合所述多肽和组合物的抗体,特别是包含核酸分子,载体,宿主细胞,多肽或抗体的诊断组合物 现在申请。 此外,提供了使用本发明的多肽检测甲基化DNA,特别是在肿瘤组织或肿瘤细胞中的方法和用途。