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    • 94. 发明专利
    • Comparative type two-dimensional and three-dimensional gel electrophoresis system
    • 比较型二维和三维凝胶电泳系统
    • JP2007171194A
    • 2007-07-05
    • JP2006343421
    • 2006-12-20
    • F Hoffmann La Roche Agエフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft
    • CURCIO MARIO
    • G01N27/447
    • G01N27/44795B01L3/5027G01N27/44773G01N27/44782
    • PROBLEM TO BE SOLVED: To provide device for separating complex protein sample based on two-dimensional gel electrophoresis, and more robust/easier method of establishing more homogeneous conditions with respect to parallel-processed gel, that is, more reproducible comparison/contrast tests. SOLUTION: For the separation in this device, 1st separation based on isoelectric point in one-dimensional strip and the 2nd separation, based on molecule size in two-dimensional gel, are performed. Furthermore, the device contains at least two gel strips for the 1st separation step and corresponding gel for two-dimensional separation. Additionally, these two pieces of gel strips are allocated to one side or to both the sides of a single carrier. As a result, having at least two 1st gel strips, enables parallel-processing of at least two analytical processes. COPYRIGHT: (C)2007,JPO&INPIT
    • 要解决的问题:提供基于二维凝胶电泳分离复合蛋白质样品的装置,以及相对于平行加工凝胶建立更均匀条件的更坚固/更容易的方法,即更可再现的比较/ 对比测试。 解决方案:为了在该装置中分离,基于二维凝胶中的分子尺寸进行基于一维条中的等电点的第一次分离和第二次分离。 此外,该装置包含用于第一分离步骤的至少两个凝胶条和用于二维分离的相应凝胶。 此外,这两条凝胶条被分配到单个载体的一侧或两侧。 结果,具有至少两个第一凝胶条,能够并行处理至少两个分析过程。 版权所有(C)2007,JPO&INPIT