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1. 发明申请

US20040101933A1 Process for producing purified anthocyanin and crystalline anthocyanin 失效
标题翻译：生产纯化花青素和结晶花青素的方法
公开(公告)号：US20040101933A1
公开(公告)日：2004-05-27
申请号：US10380056
申请日：2003-03-11
发明人： Hitoshi Matsumoto , Satoshi Hanamura , Masao Hirayama
IPC分类号： C12P019/60 , C07H017/00
CPC分类号： C07H17/00 , C07H17/065 , C09B61/00 , C12P19/14 , C12P19/44
摘要： Provided are a process for producing purified anthocyanidin glucoside in which a rhamnose end of anthocyanidin rutinoside is cleaved using rhamnosidase to convert the anthocyanidin rutinoside component into anthocyanidin glucoside, the anthocyanidin glucoside component being then purified and isolated; or a crystalline anthocyanidin glucoside salt obtained by further crystallizing the purified anthocyanidin glucoside and a process for producing the same. Also provided are a process for producing purified anthocyanidin rutinoside in which a glucose end of anthocyanidin glucoside is cleaved using null-glucosidase to degrade and remove the end, the anthocyanidin rutinoside component being then purified and isolated; or a crystalline anthocyanidin rutinoside salt obtained by further crystallizing the purified anthocyanidin rutinoside and a process for producing the same.
摘要翻译：提供了使用鼠李糖苷酶将花色素芸香糖苷成分转化为花色素苷葡糖苷的花青素芸香糖苷的鼠李糖末端切割纯化花青素葡糖苷的方法，然后纯化和分离花色素苷苷组分; 或通过进一步结晶纯化的花青素葡糖苷而获得的结晶花色素苷糖苷及其制备方法。还提供了生产纯化的花青素芸香糖苷的方法，其中使用β-葡糖苷酶切割花青素葡糖苷的葡萄糖末端以降解和除去末端，然后纯化和分离花色素芸香糖苷组分; 或通过进一步结晶纯化的花青素芸香苷获得的结晶花色素芸香糖苷盐及其制备方法。

2. 发明申请

US20040018597A1 Methods for making polyketides 审中-公开
标题翻译：聚酮化合物的制备方法
公开(公告)号：US20040018597A1
公开(公告)日：2004-01-29
申请号：US10400092
申请日：2003-03-25
发明人： Chaitan Khosla , Janice Lau , Nicola L. Pohl
IPC分类号： C12P019/60 , C12N009/10 , C12N001/21 , C12N015/74
CPC分类号： C12N15/52 , C12P19/62
摘要： The stereochemical centers of a polyketide can be changed by replacement of ketosynthase domains in the polyketide synthase (PKS) enzyme that produces the polyketide. The specificity of the AT domains of a PKS is determined by a hypervariable region that can be replaced or altered to change the specificity of the AT domain from a naturally occurring extender unit to another naturally or non-naturally occurring extender unit. Non-naturally occurring extender units, including methylmalonyl N-acetyl cysteamine thioester can be incorporated into polyketides in recombinant host cells or in cell-free systems to make polyketides.
摘要翻译：聚酮化合物的立体化学中心可以通过在产生聚酮化合物的聚酮化合物合酶（PKS）酶中置换酮解酶结构域来改变。 PKS的AT结构域的特异性由可以被替换或改变的高变区确定，以将AT结构域从天然存在的延伸单元特异性改变为另一天然或非天然存在的延伸单元。包括甲基丙二酰基N-乙酰基半胱胺硫代酯在内的非天然存在的增量单元可以掺入重组宿主细胞中的聚酮化合物或无细胞系统中以制备聚酮化合物。

3. 发明申请

US20030203425A1 Hybrid glycosylated products and their production and use 审中-公开
公开(公告)号：US20030203425A1
公开(公告)日：2003-10-30
申请号：US10257549
申请日：2003-03-25
发明人： Peter Francis Leadlay , James Staunton , Sabine Gaisser
IPC分类号： C12P021/06 , C12P019/60 , C12P019/04 , C12N009/10
CPC分类号： C07H17/08 , C12N9/1048 , C12N15/52 , C12P19/62
摘要： The present invention relates to hybrid glycosylated products, and in particular, to natural products such as polyketides and glycopeptides, and to processes for their preparation. The invention is particularly concerned with recombinant cells in which a cloned microbial glycosyltransferase can be conveniently screened for its ability to generate specific glycosylated derivatives when supplied with polyketide, peptide, or polyketide-peptides as substrates. The invention demonstrates that cloned glycosyltransferases when rapidly screened for their ability to attach a range of activated sugars to a range of exogenously supplied or endogenously generated aglycone templates, show a surprising flexibility towards both aglycone and sugar substrates, and that this process allows the production of glycosylated polyketides in good yield. This overcomes the problem not only of supplying novel sugar attachments to individual polyketides, including polyketides altered by genetic engineering, but also of increasing the diversity of polyketide libraries by combinatorial attachment of sugars.

4. 发明申请

US20040241766A1 Carbamazepine assay 审中-公开
标题翻译：卡马西平测定法
公开(公告)号：US20040241766A1
公开(公告)日：2004-12-02
申请号：US10737112
申请日：2003-12-16
发明人： Sara Rathman Zwart , Robert McMahon
IPC分类号： G01N033/53 , C12P019/60
CPC分类号： G01N33/94
摘要： Methods and assays for detecting ureido group-containing compounds and quantifying levels of these compounds in a sample are described herein.
摘要翻译：本文描述了用于检测含脲基化合物并测定样品中这些化合物的水平的方法和测定法。

5. 发明申请

US20040203119A1 Cell-free synthesis of polyketides 有权
公开(公告)号：US20040203119A1
公开(公告)日：2004-10-14
申请号：US10788650
申请日：2004-02-27
发明人： Chaitan Khosla , Rembert Pieper , Guanglin Luo , David E. Cane
IPC分类号： C12P019/60 , C07H021/04 , C12N009/10 , C12N001/21 , C12N015/74
CPC分类号： C07H17/08 , C12N15/52 , C12P17/06 , C12P17/08
摘要： Cell-free systems which effect the production of polyketides employing modular polyketide synthases are described. Libraries of new and/or known polyketides may also be produced in cell-free systems employing aromatic PKS, modular PKS or both.

6. 发明申请

US20040161828A1 Gene cluster for production of the enediyne antitumor antibiotic C-1027 有权
标题翻译：用于生产恩二抗抗生素C-1027的基因簇
公开(公告)号：US20040161828A1
公开(公告)日：2004-08-19
申请号：US10159257
申请日：2002-05-31
申请人： The Regents of the University of California
发明人： Ben Shen , Wen Liu , Stephen D. Christenson , Scott Standage
IPC分类号： C12P019/60 , C12P019/02 , C12N009/02 , C12N009/06 , C07H021/04 , C12P021/02 , C12N001/21
CPC分类号： C12P19/26 , C12N15/52 , C12P13/04 , C12P17/14 , C12P19/44
摘要： This invention provides nucleic acid sequences and characterization of the gene cluster responsible for the biosynthesis of the enediyne C-1027 (produced by Streptomyces globisporus). Methods are provided for the biosynthesis of enediynes, enediyne analogs and other biological molecules.
摘要翻译：本发明提供负责烯二烯C-1027（由全球链球菌（Streptomyces globisporus）生产）的生物合成的基因簇的核酸序列和表征。提供了用于烯二炔，烯二炔类似物和其他生物分子的生物合成的方法。

7. 发明申请

US20030073824A1 DNA encoding methymycin and pikromycin 审中-公开
标题翻译：编码甲氧西林和嘌呤霉素的DNA
公开(公告)号：US20030073824A1
公开(公告)日：2003-04-17
申请号：US09988384
申请日：2001-11-19
发明人： David H. Sherman , Hung-Wen Liu , Yongquan Xue , Lishan Zhao
IPC分类号： C07H017/08 , C07H021/04 , C12P019/60 , C12P021/02 , C12N001/21 , C12N015/74
CPC分类号： C12P7/42 , C12N15/52 , C12P19/62
摘要： A biosynthetic gene cluster for methymycin and pikromycin as well as a biosynthetic gene cluster for desosamine is provided.
摘要翻译：提供了一种生物合成基因簇，用于甲氧西林和菲古洛霉素，以及一种用于脱砷的生物合成基因簇。

8. 发明申请

US20040203015A1 Recombinant genes for polyketide modifying enzymes 有权
标题翻译：聚酮修饰酶的重组基因
公开(公告)号：US20040203015A1
公开(公告)日：2004-10-14
申请号：US10611442
申请日：2003-06-30
发明人： C. Richard Hutchinson , Leonard Katz , Ralph Reid , Zhihao Hu , Hugo Gramajo
IPC分类号： C12Q001/68 , C07H021/04 , C12P019/60 , C12N009/10 , C12N015/74
CPC分类号： C12N9/0077 , C12N15/52
摘要： Materials and methods to produce modified polyketides are disclosed. The biosynthesis, transfer and regulator genes for various sugars to effectuate polyketide modification are disclosed.
摘要翻译：公开了生产改性聚酮化合物的材料和方法。公开了用于各种糖的生物合成，转移和调节基因以实现聚酮化合物修饰。

9. 发明申请

US20040087003A1 Methods and cells for improved production of polyketides 审中-公开
标题翻译：用于改善聚酮化合物生产的方法和细胞
公开(公告)号：US20040087003A1
公开(公告)日：2004-05-06
申请号：US10461857
申请日：2003-06-13
发明人： Zhihao Hu , C. Richard Hutchinson
IPC分类号： C12N001/21 , C12P019/60
CPC分类号： C12N9/1029 , C12N9/16 , C12N15/52
摘要： Described are host cells that contain a polyketide synthase gene and a thioesterase II gene, where the polyketide synthase gene has been modified to prevent utilization of its native starter unit for its expressed polyketide synthase. Also described are host cells containing a polyketide synthase gene and an endogenous thioesterase II gene, where the activity of the endogenous thioesterase II gene product has been decreased or eliminated. Methods for culturing these cells to produce polyketides are also provided, as are the polyketides produced.
摘要翻译：描述了含有聚酮化合物合酶基因和硫酯酶II基因的宿主细胞，其中聚酮化合物合酶基因已被修饰以防止其天然起始单位用于其表达的聚酮化合物合酶。还描述了含有聚酮化合物合酶基因和内源性硫酯酶II基因的宿主细胞，其中内源性硫酯酶II基因产物的活性已经降低或消除。还提供培养这些细胞以产生聚酮化合物的方法，以及所生产的聚酮化合物。

10. 发明申请

US20040029234A1 Enhanced 2-keto-l-gulonic acid production 审中-公开
标题翻译：增强的2-酮-L-古洛糖酸生产
公开(公告)号：US20040029234A1
公开(公告)日：2004-02-12
申请号：US10343359
申请日：2003-07-18
发明人： Manoj Kumar , Fernando Valle , Veronique A. Dartois , James A. Hoch
IPC分类号： C12P019/60 , C12N015/74 , C12N001/21
CPC分类号： C12P7/60 , C07K14/24 , C07K14/26
摘要： A method for enhancing a host cell's biosynthetic production of 2-KLG is described. Such method comprises selecting a host cell that has an at least partially intracellular synthetic pathway which utilizes 2,5-DKG to produce 2-KLG; increasing the transport of said 2,5-DKG into said host cell while maintaining the integrity of the host cell; culturing the host cell to produce said 2,5-DKG; and producing 2-KLG. The transport of the 2,5-DKG is increased by transforming into the host cell DNA encoding for one or more enzymes transporting the 2,5-DKG into the host cell.
摘要翻译：描述了增强宿主细胞2-KLG生物合成生产的方法。这种方法包括选择具有至少部分细胞内合成途径的宿主细胞，其利用2,5-DKG产生2-KLG; 增加所述2,5-DKG进入所述宿主细胞的同时保持宿主细胞的完整性; 培养宿主细胞以产生所述2,5-DKG; 并生产2-KLG。通过将编码一种或多种将2,5-DKG转运到宿主细胞的酶的宿主细胞DNA转化成2,5-DKG的转运来增加。

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