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1. 发明授权

US06428999B1 Sphingolipid ceramide N-deacylase, methods for producing sphingolipids and sphingolipid derivatives, and spingolipid ceramide N-deacylase gene 失效
标题翻译：鞘氨醇神经酰胺N-脱酰酶，鞘脂和鞘脂衍生物的制造方法，和鞘脂神经酰胺N-脱酰酶基因
公开(公告)号：US06428999B1
公开(公告)日：2002-08-06
申请号：US09160036
申请日：1998-09-25
申请人： Makoto Ito , Toyohisa Kurita , Katsuhiro Kita , Noriyuki Sueyoshi , Susumu Mitsutake , Masanori Fujita , Nozomu Okino , Hiroyuki Izu , Ikunoshin Kato
发明人： Makoto Ito , Toyohisa Kurita , Katsuhiro Kita , Noriyuki Sueyoshi , Susumu Mitsutake , Masanori Fujita , Nozomu Okino , Hiroyuki Izu , Ikunoshin Kato
IPC分类号： C12N980
CPC分类号： C07H15/10 , C07F9/10 , C07H15/04 , C12N9/80 , C12P13/02 , C12P19/26
摘要： The present invention relates to a novel sphingolipid ceramide N-deacylase (SCDase) having a wide substrate specificity; a method for enzymatically producing a lysosphingolipid or a sphingolipid derivative using the SCDase which is useful in the fields of medicine, carbohydrate engineering, cell engineering, and the like; the lysosphingolipid or sphingolipid derivative obtained by this production method; a gene which encodes a polypeptide having an SCDase activity useful in sphingolipid technology; a method for industrially producing a polypeptide having an SCDase deacylase activity and a recombinant polypeptide thereof using a transformant to which the gene is introduced; a probe or primer which hybridizes to the gene; and an antibody or a fragment thereof which specifically binds to the polypeptide.
摘要翻译：本发明涉及具有宽底物特异性的新型鞘脂神经酰胺N-脱酰酶（SCDase）; 使用可用于医药，碳水化合物工程，细胞工程等领域的SCDase酶促产生溶血神经鞘脂或鞘脂衍生物的方法; 通过该制造方法得到的溶血鞘脂或鞘脂衍生物; 编码具有可用于神经鞘脂技术的SCDase活性的多肽的基因; 使用引入了该基因的转化体的工业生产具有SCD酶脱酰酶活性的多肽的方法及其重组多肽; 与基因杂交的探针或引物; 以及特异性结合多肽的抗体或其片段。

2. 发明授权

US06271015B1 Fatty-acid amide hydrolase 失效
标题翻译：脂肪酸酰胺水解酶
公开(公告)号：US06271015B1
公开(公告)日：2001-08-07
申请号：US08743168
申请日：1996-11-04
申请人： Norton B. Gilula , Benjamin F. Cravatt , Richard A. Lerner
发明人： Norton B. Gilula , Benjamin F. Cravatt , Richard A. Lerner
IPC分类号： C12N980
CPC分类号： C07C45/45 , C07C49/227 , C07C57/03 , C07C233/09 , C07C327/22 , C07F9/5407 , C12N9/80 , C12N9/99 , C12P7/6418
摘要： The soporific activity of cis-9,10-octadecenoamide and other soporific fatty acid primary(amides is neutralized by hydrolysis in the presence of fatty-acid amide hydrolase (FAAH). Hydrolysis of cis-9,10-octadecenoamide by FAAH leads to the formation of oleic acid, a compound without soporific activity. FAAH has be isolated and the gene encoding FAAH has been cloned, sequenced, and used to express recombinant FAAH. Inhibitors of FAAH are disclosed to block the hydrolase activity.
摘要翻译：顺式-9,10-十八烯酰胺和其他多孔脂肪酸一级（酰胺通过在脂肪酸酰胺水解酶（FAAH）存在下水解中和）的活性，通过FAAH水解顺-9,10-十八烯酰胺导致形成油酸，一种没有孢子活性的化合物，FAAH被分离，编码FAAH的基因已被克隆，测序并用于表达重组FAAH。公开了FAAH抑制剂以阻断水解酶活性。

3. 发明授权

US06548285B1 Polynucleotides encoding Aspergillus Niger and Penicillium Chrysogenum acetamidases and methods of use as selectable markers 失效
标题翻译：编码黑曲霉和产黄青霉乙酰胺酶的多核苷酸和用作选择性标记的方法
公开(公告)号：US06548285B1
公开(公告)日：2003-04-15
申请号：US08809955
申请日：1997-04-03
申请人： Bart W. Swinkels , Gerardus C. M. Selten , Janna G. Bakhuis , Roelof A. L. Bovenberg , Adrianus W. H. Vollebregt
发明人： Bart W. Swinkels , Gerardus C. M. Selten , Janna G. Bakhuis , Roelof A. L. Bovenberg , Adrianus W. H. Vollebregt
IPC分类号： C12N980
CPC分类号： C12N9/80
摘要： The present invention discloses novel amdS genes from fungi previously not known to contain an amdS gene, such as Aspergillus niger and Penicllium chrysogenum. The novel amdS genes can be used as homologous selectable marker genes in the transformation of these fungi. Alternatively, the cloned amdS genes can be used to inactivate the endogenous copy of the gene in order to reduce the background in transformation experiments.
摘要翻译：本发明公开了以前不知道含有amdS基因的真菌的新型amdS基因，如黑曲霉和产黄青霉。这些新型amdS基因可以用作这些真菌转化中的同源选择标记基因。或者，克隆的amdS基因可用于灭活基因的内源拷贝，以减少转化实验中的背景。

4. 发明授权

US06180388B2 Enzymes and micro organisms with amidase activity which hydrolyze polyamides 失效
标题翻译：具有水解聚酰胺的酰胺酶活性的酶和微生物
公开(公告)号：US06180388B2
公开(公告)日：2001-01-30
申请号：US09000040
申请日：1998-04-03
申请人： Jo{umlaut over (e)}l Crouzet , Didier Faucher , Olivier Favre-Bulle , Catherine Jourdat , Dominique Petre , J{acute over (e)}r{circumflex over (o)}me Pierrard , Denis Thibaut , Carole Guitton
发明人： Jo{umlaut over (e)}l Crouzet , Didier Faucher , Olivier Favre-Bulle , Catherine Jourdat , Dominique Petre , J{acute over (e)}r{circumflex over (o)}me Pierrard , Denis Thibaut , Carole Guitton
IPC分类号： C12N980
CPC分类号： C12R1/01 , C12N9/80
摘要： The present invention relates to a process for the enzymatic hydrolysis of polyamides 6.6 to give adipic acid monomers and hexamethylenediamine monomers. The present invention further relates to an enzyme with amidase activity particularly towards substrates of the oligomer type derived from PA 6.6 and/or PA 6, said enzyme being characterized in that it consists of a peptide sequence corresponding to SEQ ID NO: 1 in the attached sequence listing and/or at least one polypeptide homologous to this sequence. The invention further relates to the DNA coding for said enzyme and to the biological precursors thereof The invention further relates to the microorganisms capable of producing this enzyme and to the hydrolysis process in which this enzyme and/or these microorganisms are applied.
摘要翻译：本发明涉及聚酰胺6.6的酶水解以得到己二酸单体和六亚甲基二胺单体的方法。本发明还涉及具有酰胺酶活性的酶，特别是衍生自PA 6.6和/或PA 6的低聚物类型的底物，所述酶的特征在于其由相应于所附载体中SEQ ID NO：1的肽序列组成序列表和/或与该序列同源的至少一种多肽。本发明还涉及编码所述酶及其生物前体的DNA。本发明还涉及能够产生该酶的微生物和其中施用该酶和/或这些微生物的水解过程。

5. 发明授权

US06830919B1 Ceramidase gene 失效
标题翻译： Ceramidase基因
公开(公告)号：US06830919B1
公开(公告)日：2004-12-14
申请号：US09937521
申请日：2001-09-26
申请人： Makoto Ito
发明人： Makoto Ito
IPC分类号： C12N980
CPC分类号： C07K16/40 , C12N9/80 , C12Q1/34 , C12Y305/01023 , G01N33/573 , Y10S435/81
摘要： A neutral/alkaline ceramidase derived from a mammal; an antibody specifically binding thereto; a probe and primer which are capable of specifically hybridizing thereto; a method for producing the ceramidase by a genetic engineering means; a method for detecting the ceramidase or the gene; and a method of controlling an amount of a ceramide in a cell and/or in a tissue. The present invention is useful as a reagent for lipid engineering for analyzing a structure, functions, and the like of a ceramide, and in its applications to diseases associated with the ceramide metabolism.
摘要翻译：衍生自哺乳动物的中性/碱性神经酰胺酶; 与之结合的抗体; 能与其特异性杂交的探针和引物; 通过遗传工程方法制备神经酰胺酶的方法; 检测神经酰胺酶或基因的方法; 以及控制细胞和/或组织中神经酰胺量的方法。本发明作为用于分析神经酰胺的结构，功能等的脂质体工程试剂及其与神经酰胺代谢相关的疾病的应用是有用的。

6. 发明授权

US06596528B2 Heat-stable D-aminoacylase 失效
公开(公告)号：US06596528B2
公开(公告)日：2003-07-22
申请号：US09921156
申请日：2001-08-02
申请人： Shinji Tokuyama , Akinobu Matsuyama
发明人： Shinji Tokuyama , Akinobu Matsuyama
IPC分类号： C12N980
CPC分类号： C12R1/465 , C12N9/80 , C12P13/04
摘要： The present invention provides a novel D-aminoacylase, as well as method for producing a D-amino acid using the same. In order to achieve the above objective, the present inventors have succeeded in purifying heat-stable D-aminoacylase from microorganisms belonging to the genus Streptomyces by combining various purification methods. Furthermore, the present inventors found that the purified heat-stable D-aminoacylase is useful in industrial production of D-amino acids. By utilizing the heat-stable D-aminoacylase, it is possible to readily and efficiently produce the corresponding D-amino acids from N-acetyl-DL-amino acids (for example, N-acetyl-DL-methionine, N-acetyl-DL-valine, N-acetyl-DL-tryptophan, N-acetyl-DL-phenylalanine, N-acetyl-DL-alanine, N-acetyl-DL-leucine, and so on).

7. 发明授权

US06528300B2 Glutaryl cephalosporin amidase from Pseudomonas diminuta BS-203 失效
标题翻译：来自Pseudomonas diminuta BS-203的甲基头孢菌素酰胺酶
公开(公告)号：US06528300B2
公开(公告)日：2003-03-04
申请号：US09957960
申请日：2001-09-21
申请人： Ross Binder , Joanne L. Brown , Thomas J. Franceschini , William V. Burnett , Michael Politino , Suo Win Liu , Sean M. Tonzi
发明人： Ross Binder , Joanne L. Brown , Thomas J. Franceschini , William V. Burnett , Michael Politino , Suo Win Liu , Sean M. Tonzi
IPC分类号： C12N980
CPC分类号： C07K14/21 , C12N9/80 , C12P35/02
摘要： The invention provides a glutaryl 7-ACA amidase from Pseudomonas diminuta strain BS-203, which catalyzes the hydrolysis of 7-&bgr;-(4-carboxybutanamido)-cephalosporanic acid to 7-aminocephalosporanic acid and glutaric acid. The invention also provides nucleic acid sequences, vectors, and host cells useful in the production of this amidase. The glutaryl 7-ACA amidase can be used for the preparation of 7-aminocephalosporanic acid.
摘要翻译：本发明提供了来自减毒假单胞菌菌株BS-203的戊二酰7-ACA酰胺酶，其催化7-β-（4-羧基丁酰氨基） - 头孢烷酸至7-氨基头孢烷酸和戊二酸的水解。本发明还提供了可用于生产该酰胺酶的核酸序列，载体和宿主细胞。戊二酰7-ACA酰胺酶可用于制备7-氨基头孢烷酸。

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