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1. 发明授权

US06838251B1 Method for determining the coagulation potential of a plasma sample 有权
标题翻译：确定血浆样品凝固电位的方法
公开(公告)号：US06838251B1
公开(公告)日：2005-01-04
申请号：US09601073
申请日：1999-02-01
申请人： Thomas Exner
发明人： Thomas Exner
IPC分类号： G01N33/86 , C12Q1/56
CPC分类号： C12Q1/56 , G01N2333/462
摘要： A method of determining the coagulation potential of a plasma sample be pre-incubating the plasma sample with a reagent such that endogenous protein C in the plasma is at least partially converted into activated protein C by the reagent, adding factor Xa which is progressively inactivated by antithrombin III/heparin cofactor 2 during the preincubation, adding an exogenous reagent which activates factor X to Xa or prothrombin to thrombin in a factor V-dependent manner, monitoring a reaction indicative of the rate of coagulation of the plasma sample, comparing that rate of coagulation control, or the equivalent rate determined for an individual without impaired coagulation control, or the equivalent rate determined for the plasma sample in the absence of protein C activator, and determining the coagulation potential of the plasma sample from one or other of the compairsons.
摘要翻译：确定血浆样品的凝血电位的方法是用试剂预先培养血浆样品，使得血浆中的内源性蛋白C至少部分地被试剂转化成活化的蛋白质C，加入通过下述方法逐渐失活的添加因子Xa 抗凝血酶III /肝素辅因子2，加入外因试剂，以因子V依赖性方式将X因子激活至Xa或凝血酶凝血酶，监测血浆样品凝血速率的反应，凝血控制或对于没有受损凝血控制的个体而确定的等效速率，或者在不存在蛋白C激活剂的情况下为血浆样品确定的等效速率，以及确定来自一个或其它比较物的血浆样品的凝固电位。

2. 发明授权

US6051434A Activated protein C resistance test 失效
标题翻译：活化蛋白C电阻测试
公开(公告)号：US6051434A
公开(公告)日：2000-04-18
申请号：US776897
申请日：1997-04-24
申请人： Thomas Exner
发明人： Thomas Exner
IPC分类号： G01N33/86
CPC分类号： G01N33/86 , G01N2333/4613 , G01N2333/4616 , G01N2333/96444 , G01N2333/96461 , G01N2333/96463 , Y10T436/25
摘要： The method for determining functional activity of protein C in a human plasma sample includes incubating the human plasma sample with exogenous reagents that activate factor V and a common pathway of the blood coagulation mechanism through factor X, with activated exogenous protein C and with components that are necessary for efficient clotting of the human plasma sample, or incubating the human plasma sample with exogenous reagents that induce the presence of thrombin in a factor V dependent manner, with activated exogenous protein C and with components that are necessary for efficient clotting of the human plasma sample; monitoring a reaction indicative of a potential rate of coagulation of the plasma sample and comparing the resulting potential rate of coagulation with an equivalent rate for normal patient, or comparing the resulting potential rate of coagulation with an equivalent rate determined for the plasma sample in the absence of activated exogenous protein C; and determining the functional activity of the free protein C from this comparison.
摘要翻译： PCT No.PCT / AU95 / 00474 Sec。 371日期1997年04月24日 102（e）日期1997年4月24日PCT提交1995年8月7日PCT公布。公开号WO96 / 04560 日期1996年2月15日人血浆样品中蛋白C的功能活性测定方法包括通过激活的外源蛋白C将人血浆样品与激活因子V的外源试剂和通过因子X的凝血机制的共同途径进行孵育以及用于有效凝固人血浆样品所必需的成分，或用活化的外源蛋白C以及有效成分所必需的成分，以以因子V依赖性方式诱导凝血酶存在的外源试剂孵育人血浆样品人血浆样品凝结; 监测指示血浆样品的潜在凝血速率的反应并将所得到的潜在凝血速率与正常患者的等效速率进行比较，或将所得到的潜在凝血速率与在不存在的血浆样品中测定的等效速率进行比较的活化外源蛋白C; 并从该比较中确定游离蛋白C的功能活性。

3. 发明授权

US5601995A Apparatus and method for detecting coagulation in blood samples 失效
标题翻译：血液样品中凝血检测装置及方法
公开(公告)号：US5601995A
公开(公告)日：1997-02-11
申请号：US397158
申请日：1995-03-03
申请人： Thomas Exner
发明人： Thomas Exner
IPC分类号： G01N33/52 , A61B5/145 , A61B5/1459 , G01N27/04 , G01N33/49 , G01N33/86 , C12Q1/56 , G01N31/22
CPC分类号： G01N33/4905 , G01N33/86
摘要： The method and apparatus are useful for determining a propensity of a blood sample to change from a liquid state to a coagulated state and additionally for measuring the propensity of a coagulated blood sample to lyse. The method includes providing a porous sheet, at least one surface of which contacts an impervious layer; applying the blood sample to an exposed surface of the porous sheet so that the blood sample spreads through a part of the porous sheet; and after the applying of the blood sample to the porous sheet, measuring at least one of a spreading extent and a spreading rate of the blood sample in the porous sheet by measuring either an optical property, an electrical conductivity across the porous sheet, an electrical potential across the porous sheet and an electrical resistance of the porous sheet to determine the propensity of the blood sample to coagulate. The porous sheet can also be impregnated with a clotting agent or a lytic agent to affect the clotting or lysing of the blood sample.
摘要翻译： PCT No.PCT / AU93 / 00459 Sec。 371日期1995年3月3日 102（e）1995年3月3日PCT PCT 1993年9月6日PCT公布。出版物WO94 / 06007 日期1994年3月17日该方法和装置可用于确定血液样品从液体状态转变为凝固状态的倾向，另外用于测量凝固血液样品裂解的倾向。该方法包括提供其至少一个表面接触不透水层的多孔片材; 将血液样品施加到多孔片的暴露表面，使得血液样品通过多孔片的一部分扩散; 并且在将所述血液样品施加到所述多孔片材之后，通过测量所述多孔片材中的光学性质，导电性，电气性的电学方法来测量所述多孔片材中的血液样品的扩散程度和铺展速度中的至少一个跨越多孔片的电位和多孔片的电阻以确定血液样品凝固的倾向。多孔片也可以用凝固剂或溶解剂浸渍以影响血液样品的凝固或裂解。

4. 发明申请

US20060228765A1 Method for detecting procoagulant phospholipid 审中-公开
标题翻译：检测促凝血磷脂的方法
公开(公告)号：US20060228765A1
公开(公告)日：2006-10-12
申请号：US11384258
申请日：2006-03-21
申请人： Thomas Exner
发明人： Thomas Exner
IPC分类号： C12Q1/56 , C12N9/20
CPC分类号： G01N33/86 , H03L7/22
摘要： The present invention relates to a method for determining the amount of procoagulant phospholipid in a sample, said method comprising steps (i) to (iii) performed in the following order: (i) forming an admixture of the sample and a substrate plasma which has been rendered free or substantially free of procoagulant phospholipid sufficient to at least reduce the capacity of the substrate plasma to coagulate, wherein said substrate plasma has been rendered free or substantially free of procoagulant phospholipid by treatment with a phospholipase; (ii) contacting the admixture with a reagent for activating coagulation of plasma in conditions were procoagulant phospholipids is the rate limiting component of the mixture; and (iii) determining the clotting time of the admixture.
摘要翻译：本发明涉及一种用于测定样品中促凝血磷量的方法，所述方法包括以下列顺序进行的步骤（i）至（iii）：（i）形成样品与底物等离子体的混合物，其具有被释放或基本上不含足以至少降低底物血浆凝结能力的促凝血磷脂，其中所述底物血浆已经通过用磷脂酶处理而游离或基本上不含促凝血磷脂; （ii）将混合物与用于激活血浆凝固的试剂在条件下接触是促凝血磷脂是混合物的速率限制组分; 和（iii）确定混合物的凝固时间。

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