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1. 发明公开

EP3943192A1 MICROFLUIDIC ANALYTICAL DEVICE 审中-公开
公开(公告)号：EP3943192A1
公开(公告)日：2022-01-26
申请号：EP21181242.5
申请日：2018-06-21
申请人： Lightcast Discovery Ltd
发明人： ISAAC, Thomas Henry , CUNHA, Pedro , SHERIDAN, Eoin , LOVE, David , PALMER, Rebecca , KELLY, Douglas J , PODD, Gareth
IPC分类号： B01L3/00 , C12Q1/6869
摘要： A device for investigating a nucleic acid analyte characterised by comprising: • a first zone comprising an attachment site to which the analyte is attached; a first pathway for causing a first fluid medium to flow over the attachment site thereby allowing the nucleic acid to be progressively pyrophosphorolysed into its constituent nucleoside triphosphates; a second pathway for removing the nucleoside triphosphates from around the attachment site and a means for creating in the second pathway a second medium comprised of aqueous microdroplets in a water-immiscible carrier; • a microdroplet manipulation zone for manipulating the microdroplets using optically-mediated electrowetting comprised of: • a first composite wall comprised of • a first transparent substrate • a first transparent conductor layer on the substrate having a thickness in the range 70 to 250nm; • a photoactive layer activated by electromagnetic radiation in the wavelength range 400-1000nm on the conductor layer having a thickness in the range 300-1000nm and • a first dielectric layer on the conductor layer having a thickness in the range 120 to 160nm; • a second composite wall comprised of • a second substrate; • a second conductor layer on the substrate having a thickness in the range 70 to 250nm and • optionally a second dielectric layer on the conductor layer having a thickness in the range 120 to 160nm; • wherein the exposed surfaces of the first and second dielectric layers are disposed less than 10µm apart to define a microfluidic space adapted to contain microdroplets; • an A/C source to provide a voltage across the first and second composite walls connecting the first and second conductor layers; • a source of first electromagnetic radiation having an energy higher than the bandgap of the photoexcitable layer adapted to impinge on the photoactive layer to induce corresponding ephemeral first electrowetting locations on the surface of the first dielectric layer; • means for manipulating the points of impingement of the electromagnetic radiation on the photoactive layer so as to vary the disposition of the ephemeral electrowetting locations thereby creating at least one first electrowetting pathway along which the microdroplets may be caused to move; • an detection zone disposed downstream of the microdroplet manipulation zone or integral therewith and • a fluorescence or Raman-scattering detection system comprising a second source of electromagnetic radiation adapted to impinge on the microdroplets in the detection zone and a detector for detecting fluorescence or Raman-scattering emitted therefrom.

2. 发明公开

EP4450162A2 MICRODROPLET MANIPULATION DEVICE 审中-公开
公开(公告)号：EP4450162A2
公开(公告)日：2024-10-23
申请号：EP24195868.5
申请日：2018-06-21
申请人： Lightcast Discovery Ltd
发明人： ISAAC, Thomas Henry , CUNHA, Pedro , SHERIDAN, Eoin , LOVE, David , PALMER, Rebecca , KELLY, Douglas J. , PODD, Gareth
IPC分类号： B01L3/00
CPC分类号： B01L3/502792 , B01L2200/067320130101 , B01L2300/086420130101 , B01L2300/088720130101 , B01L2300/08920130101 , B01L2300/16120130101 , B01L2300/16520130101 , B01L2300/16820130101 , B01L2400/042720130101 , B01L3/50273
摘要： A device for fast manipulation of many thousands of microdroplets simultaneously using optically-mediated electrowetting is provided. The device comprising: a first composite wall comprising: a first substrate (2b); a first transparent conductor layer (3) on the first substrate (2b) having a thickness in the range 70 to 250 nm; a photoactive layer (5) activated by electromagnetic radiation in the wavelength range 400-1000 nm on the first transparent conductor layer having a thickness in the range 300-1000 nm and a first dielectric layer (6) on the photoactive layer having a thickness in the range of 120 to 160nm; and a first anti-fouling layer (7) on the first dielectric layer; a second composite wall comprising: a second substrate (2a); a second conductor layer (3) on the second substrate having a thickness in the range 70 to 250 nm; and a second dielectric layer (6) on the second conductor layer; and a second anti-fouling layer (7) on the second dielectric layer the device further comprising: one or more spacers for holding the first and second walls apart by a pre-determined amount to define a microfluidic space adapted to contain microdroplets, wherein the spacer comprises a bead, a pillar or a ridge; an A/C source (4) to provide a voltage of between 10V and 50V across the first and second composite walls connecting the first and second conductor layers so as to be below the dielectric breakdown voltage of the first and second dielectric layers (6); at least one source of electromagnetic radiation (8) having an energy higher than the bandgap of a photoexcitable layer adapted to impinge on the photoactive layer (5) to induce corresponding ephemeral electrowetting locations (11) on the surface of the first dielectric layer (6); and a microprocessor (12) for manipulating points of impingement of the electromagnetic radiation on the photoactive layer so as to vary the disposition of the ephemeral electrowetting locations (11) thereby creating at least one electrowetting pathway along which microdroplets may be caused to move; wherein the device is configured to performing chemical analyses carried out on multiple analytes simultaneously.

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