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1. 发明申请

US20120107959A1 BAIT CHEMISTRIES IN HYDROGEL PARTICLES FOR SERUM BIOMARKER ANALYSIS 审中-公开
标题翻译：用于血浆生物标记分析的氢化物颗粒中的化学化学
公开(公告)号：US20120107959A1
公开(公告)日：2012-05-03
申请号：US13379345
申请日：2010-06-21
申请人： Lance Liotta , Virginia Espina , Alessandra Luchini , Davide Tamburro
发明人： Lance Liotta , Virginia Espina , Alessandra Luchini , Davide Tamburro
IPC分类号： G01N21/75
CPC分类号： C07D311/82 , B01J20/3253 , B01J20/3255 , C07D251/50 , C07D279/20 , C07D403/04 , C07D471/22
摘要： This invention describes the identification of novel organic dye chemistries that can be used as affinity baits to capture proteins and other biomolecules useful in the fields of medical diagnostics, environmental science, toxicology, and infectious disease. Incorporation of unique affinity dye compounds within hydrogel capture particles improves analyte yield and preanalytical precision, and stabilizes the analyte against degradation, while increasing measurement sensitivity. The particles in this invention can be used for routine clinical testing as well as for discovery of low abundance disease biomarkers. Example hydrogel particles containing new high affinity bait chemistries were used to identify a new set of human serum biomarkers.
摘要翻译：本发明描述了可用作捕获蛋白质和其它可用于医学诊断学，环境科学，毒理学和传染病领域的其他生物分子的亲和诱饵的新型有机染料化学物质的鉴定。在水凝胶捕获颗粒中引入独特的亲和染料化合物可提高分析物的产率和分析前精度，并稳定分析物以防止降解，同时提高测量灵敏度。本发明中的颗粒可用于常规临床测试以及发现低丰度疾病生物标志物。使用含有新的高亲和力诱饵化学物质的示例性水凝胶颗粒来鉴定新的一组人血清生物标志物。

2. 发明申请

US20130085076A1 BORRELIA BURGDORFERI BACTERIAL ANTIGEN DIAGNOSIC TEST USING POLYMERIC BAIT CONTAINING CAPTURE PARTICLES 审中-公开
标题翻译： BORRELIA BURGDORFERI细菌抗原测试使用含聚合物颗粒的聚合物
公开(公告)号：US20130085076A1
公开(公告)日：2013-04-04
申请号：US13513568
申请日：2010-12-01
申请人： Temple Douglas , Alessandra Luchini , Claudia Fredolini , Davide Tamburro , Lance Liotta
发明人： Temple Douglas , Alessandra Luchini , Claudia Fredolini , Davide Tamburro , Lance Liotta
IPC分类号： G01N33/569
CPC分类号： G01N33/56911 , G01N33/54313 , G01N2333/20 , G01N2469/10 , Y02A50/57
摘要： The invention relates to both a sensitive method for the capture and detection of low-abundance Borrelia burgdorferi (Bb) bacterial antigens allowing for the diagnosis of Lyme Disease using standard immunoassays. Furthermore, this invention allows the antigen to be identified in a sample of urine, serum, or other biological fluids isolated from humans and animals. The invention provides a method to capture, concentrate, separate and specifically quantify the abundance of Bb antigens using immunoassays. The detection of Bb Outer Surface Protein A is presented as an example of the disclosed invention. High sensitivity levels, low cost and easily collected biofluids allow this technology to reach patients in clinics as well as POC applications for the early detection of Lyme disease prior to seroconversion. A kit containing necessary reagents and the method for diagnosis, monitoring or assessing lyme disease using an immunoassay such as an ELISA, western blot or RPPMA is disclosed.
摘要翻译：本发明涉及用于捕获和检测低丰度伯氏疏螺旋体（Bb）细菌抗原的敏感方法，其允许使用标准免疫测定法诊断莱姆病。此外，本发明允许在从人和动物分离的尿液，血清或其他生物流体的样品中鉴定抗原。本发明提供了使用免疫测定法捕获，浓缩，分离和特异性定量Bb抗原丰度的方法。 Bb外表面蛋白A的检测作为本发明的一个例子。高灵敏度水平，低成本和易于收集的生物流体使得该技术可以在诊所和POC应用中接触患者，以便在血清转化前早期发现莱姆病。公开了含有必需试剂的试剂盒和使用免疫测定如ELISA，western印迹或RPPMA进行诊断，监测或评估淋巴细胞疾病的方法。

3. 发明申请

US20120164749A1 Smart Hydrogel Particles for Biomarker Harvesting 有权
标题翻译：用于生物标记收获的智能水凝胶颗粒
公开(公告)号：US20120164749A1
公开(公告)日：2012-06-28
申请号：US12194360
申请日：2008-08-19
申请人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , Barney Bishop , Caterina Longo , Virginia Espina , Alexis Patanarut
发明人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , Barney Bishop , Caterina Longo , Virginia Espina , Alexis Patanarut
IPC分类号： G01N1/18 , C08F220/56 , B82Y40/00
CPC分类号： G01N33/5432 , G01N33/5436 , Y10T428/2989 , Y10T428/2998 , Y10T436/25375
摘要： Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles are changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles are made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.
摘要翻译：描述从溶液中收集分析物的捕获颗粒和使用它们的方法。捕获颗粒由具有允许分析物进入捕获颗粒的孔径的聚合物基质构成。当对颗粒施加刺激时，捕获颗粒的孔径是可变的，允许改变颗粒的孔径，使得目标分析物保留在颗粒内。捕获颗粒的聚合物基质由具有结构单体和亲和单体的共聚物制成，亲和单体具有将分析物吸引到捕获颗粒的性质。捕获颗粒可用于分离和鉴定混合物中存在的分析物。它们也可用于保护在收获时通常会降解的分析物，并将低分析物浓缩在液体中的丰度低。

4. 发明授权

US08497137B2 Smart hydrogel particles for biomarker harvesting 有权
标题翻译：用于生物标志物收获的智能水凝胶颗粒
公开(公告)号：US08497137B2
公开(公告)日：2013-07-30
申请号：US12194360
申请日：2008-08-19
申请人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , Barney Bishop , Caterina Longo , Virginia Espina , Alexis Patanarut
发明人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , Barney Bishop , Caterina Longo , Virginia Espina , Alexis Patanarut
IPC分类号： G01N33/545
CPC分类号： G01N33/5432 , G01N33/5436 , Y10T428/2989 , Y10T428/2998 , Y10T436/25375
摘要： Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles are changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles are made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.
摘要翻译：描述从溶液中收集分析物的捕获颗粒和使用它们的方法。捕获颗粒由具有允许分析物进入捕获颗粒的孔径的聚合物基质构成。当对颗粒施加刺激时，捕获颗粒的孔径是可变的，允许改变颗粒的孔径，使得目标分析物保留在颗粒内。捕获颗粒的聚合物基质由具有结构单体和亲和单体的共聚物制成，亲和单体具有将分析物吸引到捕获颗粒的性质。捕获颗粒可用于分离和鉴定混合物中存在的分析物。它们也可用于保护在收获时通常会降解的分析物，并将低分析物浓缩在液体中的丰度低。

5. 发明申请

US20110236999A1 Hydrogel Nanoparticle Based Immunoassay 有权
标题翻译：水凝胶纳米粒子免疫测定
公开(公告)号：US20110236999A1
公开(公告)日：2011-09-29
申请号：US13061507
申请日：2009-08-26
申请人： Lance A. Liotta , Alessandra Luchini , Emaneul F. Petricoin , Virginia Espina
发明人： Lance A. Liotta , Alessandra Luchini , Emaneul F. Petricoin , Virginia Espina
IPC分类号： G01N33/543 , B82Y15/00
CPC分类号： G01N33/521 , G01N33/54333 , G01N33/54346
摘要： An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. This device is useful for point of care diagnostic assays for biomedical applications and as field deployable assays for environmental, pathogen and chemical or biological threat identification.
摘要翻译：提供了在分析装置的流体流动路径内将多孔聚合物捕获纳米颗粒结合到样品采集容器中或预浸渍到多孔基质内的免疫测定装置。这种在免疫测定装置内的捕获颗粒的并入提高了灵敏度，同时在加载免疫测定装置之前消除了样品预处理的要求。优选的实施方案是含有捕获纳米颗粒的芯壳诱饵，其在一个步骤中在溶液中进行三个功能：a）分子筛分，b）目标分析物螯合和浓缩，以及c）防止降解。捕获颗粒的聚合物基质可以由具有结构单体和亲和单体的共聚物制成，亲和单体具有将分析物吸引到捕获颗粒的性质。该设备可用于生物医学应用的护理点诊断分析以及环境，病原体和化学或生物威胁鉴定的现场部署测定。

6. 发明授权

US09012240B2 Hydrogel nanoparticle based immunoassay 有权
标题翻译：基于水凝胶纳米颗粒的免疫测定
公开(公告)号：US09012240B2
公开(公告)日：2015-04-21
申请号：US13061507
申请日：2009-08-26
申请人： Lance A. Liotta , Alessandra Luchini , Emanuel F. Petricoin , Virginia Espina
发明人： Lance A. Liotta , Alessandra Luchini , Emanuel F. Petricoin , Virginia Espina
IPC分类号： G01N33/549 , G01N33/53 , G01N33/544 , G01N33/558 , G01N33/52 , G01N33/543
CPC分类号： G01N33/521 , G01N33/54333 , G01N33/54346
摘要： An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. This device is useful for point of care diagnostic assays for biomedical applications and as field deployable assays for environmental, pathogen and chemical or biological threat identification.
摘要翻译：提供了在分析装置的流体流动路径内将多孔聚合物捕获纳米颗粒结合到样品采集容器中或预浸渍到多孔基质内的免疫测定装置。这种在免疫测定装置内的捕获颗粒的并入提高了灵敏度，同时在加载免疫测定装置之前消除了样品预处理的要求。优选的实施方案是含有捕获纳米颗粒的芯壳诱饵，其在一个步骤中在溶液中进行三个功能：a）分子筛分，b）目标分析物螯合和浓缩，以及c）防止降解。捕获颗粒的聚合物基质可以由具有结构单体和亲和单体的共聚物制成，亲和单体具有将分析物吸引到捕获颗粒的性质。该设备可用于生物医学应用的护理点诊断分析以及环境，病原体和化学或生物威胁鉴定的现场部署测定。

7. 发明授权

US08382987B2 Method for harvesting nanoparticles and sequestering biomarkers 有权
标题翻译：收获纳米颗粒和螯合生物标志物的方法
公开(公告)号：US08382987B2
公开(公告)日：2013-02-26
申请号：US12194371
申请日：2008-08-19
申请人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , Barney Bishop , Francesco Meani , Claudia Fredolini , Thomas M Dunlap , Alexis Patanarut
发明人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , Barney Bishop , Francesco Meani , Claudia Fredolini , Thomas M Dunlap , Alexis Patanarut
IPC分类号： B01D39/16
CPC分类号： G01N1/405 , B01D15/00 , B01D15/02 , B01D15/34 , B01D15/3804 , B01D15/3861 , B01J20/26 , B01J20/261 , B01J20/264 , B01J20/265 , B01J2220/54 , B82Y15/00 , G01N33/54313 , G01N33/545 , Y10T436/255
摘要： Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles are changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles are made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.
摘要翻译：描述从溶液中收集分析物的捕获颗粒和使用它们的方法。捕获颗粒由具有允许分析物进入捕获颗粒的孔径的聚合物基质构成。当对颗粒施加刺激时，捕获颗粒的孔径是可变的，允许改变颗粒的孔径，使得目标分析物保留在颗粒内。捕获颗粒的聚合物基质由具有结构单体和亲和单体的共聚物制成，亲和单体具有将分析物吸引到捕获颗粒的性质。捕获颗粒可用于分离和鉴定混合物中存在的分析物。它们也可用于保护在收获时通常会降解的分析物，并将低分析物浓缩在液体中的丰度低。

8. 发明申请

US20090087346A1 Method for Harvesting Nanoparticles and Sequestering Biomarkers 有权
标题翻译：收获纳米颗粒和螯合生物标志物的方法
公开(公告)号：US20090087346A1
公开(公告)日：2009-04-02
申请号：US12194371
申请日：2008-08-19
申请人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , Barney Bishop , Francesco Meani , Claudia Fredolini , Thomas M. Dunlap , Alexis Patanarut
发明人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , Barney Bishop , Francesco Meani , Claudia Fredolini , Thomas M. Dunlap , Alexis Patanarut
IPC分类号： B01J20/24 , G01N30/00 , B01J20/26
CPC分类号： G01N1/405 , B01D15/00 , B01D15/02 , B01D15/34 , B01D15/3804 , B01D15/3861 , B01J20/26 , B01J20/261 , B01J20/264 , B01J20/265 , B01J2220/54 , B82Y15/00 , G01N33/54313 , G01N33/545 , Y10T436/255
摘要： Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles are changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles are made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.
摘要翻译：描述从溶液中收集分析物的捕获颗粒和使用它们的方法。捕获颗粒由具有允许分析物进入捕获颗粒的孔径的聚合物基质构成。当对颗粒施加刺激时，捕获颗粒的孔径是可变的，允许改变颗粒的孔径，使得目标分析物保留在颗粒内。捕获颗粒的聚合物基质由具有结构单体和亲和单体的共聚物制成，亲和单体具有将分析物吸引到捕获颗粒的性质。捕获颗粒可用于分离和鉴定混合物中存在的分析物。它们也可用于保护在收获时通常会降解的分析物，并将低分析物浓缩在液体中的丰度低。

9. 发明授权

US07935518B2 Smart hydrogel particles for biomarker harvesting 有权
标题翻译：用于生物标志物收获的智能水凝胶颗粒
公开(公告)号：US07935518B2
公开(公告)日：2011-05-03
申请号：US12033701
申请日：2008-02-19
申请人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , David Geho
发明人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , David Geho
IPC分类号： G01N33/545
CPC分类号： B01J20/26 , B01D15/34 , B01J20/265 , B01J20/28078 , B01J20/28095 , B01J2220/54 , C12Q1/6816 , G01N33/54313 , G01N33/54353 , G01N33/5436 , Y10S435/81 , Y10S436/808 , Y10T436/255 , C12Q2565/518
摘要： Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles may be changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.
摘要翻译：描述从溶液中收集分析物的捕获颗粒和使用它们的方法。捕获颗粒由具有允许分析物进入捕获颗粒的孔径的聚合物基质构成。当对颗粒施加刺激时，捕获颗粒的孔径可以变化，允许改变颗粒的孔径，使得目标分析物保留在颗粒内。捕获颗粒的聚合物基质可以由具有结构单体和亲和单体的共聚物制成，亲和单体具有将分析物吸引到捕获颗粒的性质。捕获颗粒可用于分离和鉴定混合物中存在的分析物。它们也可用于保护在收获时通常会降解的分析物，并将低分析物浓缩在液体中的丰度低。

10. 发明申请

US20090148961A1 SMART HYDROGEL PARTICLES FOR BIOMARKER HARVESTING 有权
标题翻译：用于生物标记收集的SMART HYDROGEL颗粒
公开(公告)号：US20090148961A1
公开(公告)日：2009-06-11
申请号：US12033701
申请日：2008-02-19
申请人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , David Geho
发明人： Alessandra Luchini , Lance Liotta , Emanuel Petricoin , David Geho
IPC分类号： G01N33/543 , B01J19/00 , G01N27/00 , G01N21/00 , G01N21/64 , C12M1/00 , C08J9/16
CPC分类号： B01J20/26 , B01D15/34 , B01J20/265 , B01J20/28078 , B01J20/28095 , B01J2220/54 , C12Q1/6816 , G01N33/54313 , G01N33/54353 , G01N33/5436 , Y10S435/81 , Y10S436/808 , Y10T436/255 , C12Q2565/518
摘要： Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles may be changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.
摘要翻译：描述从溶液中收集分析物的捕获颗粒和使用它们的方法。捕获颗粒由具有允许分析物进入捕获颗粒的孔径的聚合物基质构成。当对颗粒施加刺激时，捕获颗粒的孔径可以变化，允许改变颗粒的孔径，使得目标分析物保留在颗粒内。捕获颗粒的聚合物基质可以由具有结构单体和亲和单体的共聚物制成，亲和单体具有将分析物吸引到捕获颗粒的性质。捕获颗粒可用于分离和鉴定混合物中存在的分析物。它们也可用于保护在收获时通常会降解的分析物，并将低分析物浓缩在液体中的丰度低。

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