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1. 发明授权

US5571729A Process for separating complex 失效
标题翻译：分离复合物的方法
公开(公告)号：US5571729A
公开(公告)日：1996-11-05
申请号：US354264
申请日：1994-12-12
申请人： Kenji Nakamura , Taizo Hara , Hideo Katoh , Shinji Satomura
发明人： Kenji Nakamura , Taizo Hara , Hideo Katoh , Shinji Satomura
IPC分类号： G01N33/538 , G01N33/573
CPC分类号： G01N33/538 , Y10S436/824
摘要： This invention is intended to provide a process by which a complex formed by the interaction between one or more analytes to be measured and affinity substance can be more effectively separated from substances existing together therewith which tend to affect the detection of the complex, for example, free affinity substance, by using high pressure liquid chromatography (HPLC); and a process for measuring a trace component by utilizing said separating process.This invention is characterized in that a substance which has been modified with a substance capable of changing properties of the complex (a separation-improving substance) and has affinity for the complex is attached to the complex. Because of this characteristic, the invention is effective in that the position of elution of said complex in the HPLC can be freely controlled. Furthermore, it is markedly effective in that the process for measuring a trace component by utilizing the separating process of this invention makes it possible to carry out the measurement with higher precision as compared with conventional measuring processes using HPLC.
摘要翻译：本发明旨在提供一种方法，通过该方法可以将待测量的一种或多种待分析物与亲和物质之间的相互作用形成的复合物与其中存在的物质更有效地分离，这些物质倾向于影响复合物的检测，例如，游离亲和性物质，通过使用高压液相色谱（HPLC）; 以及通过利用所述分离过程来测量痕量组分的方法。本发明的特征在于，将具有能够改变复合物（分离改善物质）性质并对复合物具有亲和性的物质进行了改性的物质附着于该络合物。由于该特征，本发明的效果在于可以自由地控制HPLC中所述络合物的洗脱位置。此外，通过利用本发明的分离方法测量痕量组分的方法与使用HPLC的常规测量方法相比，可以更高精度地进行测量。

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