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1. 发明授权

US5843681A Dioxetane compounds for the chemiluminescent detection of proteases, methods of use and kits therefore 失效
标题翻译：因此，用于化学发光检测蛋白酶的二氧杂环丁烷化合物，使用方法和试剂盒
公开(公告)号：US5843681A
公开(公告)日：1998-12-01
申请号：US728990
申请日：1996-10-11
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , Alison Sparks , John C. Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , Alison Sparks , John C. Voyta
IPC分类号： G01N33/573 , C07D321/00 , C07K5/072 , C07K5/08 , C07K5/09 , C07K5/10 , C12Q1/37 , G01N33/58
CPC分类号： C07K5/06095 , C07D321/00 , C07K5/0802 , C07K5/0817 , C07K5/1002 , C12Q1/37 , G01N33/582 , Y10S530/807
摘要： 1, 2-dioxetane compounds bearing a proteolytic enzyme specific amino acid or peptide are provided, which amino acid or peptide can be removed by action of the corresponding protease. When the amino acid or peptide is removed, the 1,2-dioxetane decomposes with chemiluminescence, the generation of light providing a rapid, ultra-sensitive and convenient means for detecting the presence of the protease in the sample being inspected. The amount of light generated, or degree of chemiluminescence, can be correlated with the amount of protease present. Immunoassays, as well as DNA hybridization and DNA probe assays are provided.
摘要翻译：提供了带有蛋白水解酶特异性氨基酸或肽的1,2-二氧杂环丁烷化合物，该氨基酸或肽可以通过相应的蛋白酶的作用除去。当去除氨基酸或肽时，1,2-二氧杂环丁烷以化学发光分解，产生光，提供快速，超灵敏和方便的手段，用于检测待检样品中蛋白酶的存在。产生的光量或化学发光度可以与存在的蛋白酶的量相关。提供免疫测定，以及DNA杂交和DNA探针测定。

2. 发明授权

US5591591A Dioxetane compounds for the chemiluminescent detection of proteases, methods of use and kits therefore 失效
标题翻译：用于化学发光检测蛋白酶的二氧杂环丁烷化合物，使用方法及其试剂盒
公开(公告)号：US5591591A
公开(公告)日：1997-01-07
申请号：US385788
申请日：1995-02-09
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , Alison Sparks , John C. Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , Alison Sparks , John C. Voyta
IPC分类号： G01N33/573 , C07D321/00 , C07K5/072 , C07K5/08 , C07K5/09 , C07K5/10 , C12Q1/37 , G01N33/58 , C07K5/06
CPC分类号： C07K5/06095 , C07D321/00 , C07K5/0802 , C07K5/0817 , C07K5/1002 , C12Q1/37 , G01N33/582 , Y10S530/807
摘要： 1,2-dioxetane compounds bearing a proteolytic enzyme specific amino acid or peptide are provided, which amino acid or peptide can be removed by action of the corresponding protease. When the amino acid or peptide is removed, the 1,2-dioxetane decomposes with chemiluminescence, the generation of light providing a rapid, ultra-sensitive and convenient means for detecting the presence of the protease in the sample being inspected. The amount of light generated, or degree of chemiluminescence, can be correlated with the amount of protease present. Immunoassays, as well as DNA hybridization and DNA probe assays are provided.
摘要翻译：提供了带有蛋白水解酶特异性氨基酸或肽的1,2-二氧环乙烷化合物，该氨基酸或肽可以通过相应的蛋白酶的作用除去。当去除氨基酸或肽时，1,2-二氧杂环丁烷以化学发光分解，产生光，提供快速，超灵敏和方便的手段，用于检测待检样品中蛋白酶的存在。产生的光量或化学发光度可以与存在的蛋白酶的量相关。提供免疫测定，以及DNA杂交和DNA探针测定。

3. 发明授权

US5783381A Chemiluminescent 1,2-dioxetanes 失效
公开(公告)号：US5783381A
公开(公告)日：1998-07-21
申请号：US545174
申请日：1995-10-19
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks , John C. Voyta
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks , John C. Voyta
IPC分类号： G01N31/00 , C07D305/06 , C07D321/00 , C07F7/18 , C09K11/07 , C12Q1/34 , G01N21/78 , G01N33/52 , C12Q1/00
CPC分类号： C12Q1/34 , C07D321/00 , C07F7/1856 , G01N33/52 , Y10S435/975
摘要： Dioxetanes that can be triggered by bases are provided which can be used to detect bases in organic solvents, aqueous preparations and detect gaseous bases in the atmosphere. The dioxetanes can be used to detect the release of bases from various labels, as a means to detect the presence of a base released by physical or natural processes, to calibrate light measuring apparatus and to determine the amount of reducing or oxidizing agent present in the base.

4. 发明授权

US6132956A Chemiluminescent 1,2-dioxetanes 失效
标题翻译：化学发光的1,2-二氧杂环丁烷
公开(公告)号：US6132956A
公开(公告)日：2000-10-17
申请号：US964552
申请日：1997-11-05
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks , John C. Voyta
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks , John C. Voyta
IPC分类号： G01N31/00 , C07D305/06 , C07D321/00 , C07F7/18 , C09K11/07 , C12Q1/34 , G01N21/78 , G01N33/52 , C12Q1/00
CPC分类号： C12Q1/34 , C07D321/00 , C07F7/1856 , G01N33/52 , Y10S435/975
摘要： Dioxetanes that can be triggered by bases are provided which can be used to detect bases in organic solvents, aqueous preparations and detect gaseous bases in the atmosphere. The dioxetanes can be used to detect the release of bases from various labels, as a means to detect the presence of a base released by physical or natural processes, to calibrate light measuring apparatus and to determine the amount of reducing or oxidizing agent present in the base.
摘要翻译：提供可由碱触发的二氧杂环丁烷，可用于检测有机溶剂，含水制剂中的碱，并检测大气中的气态碱。二氧杂环丁烷可用于检测各种标记物中碱的释放，作为检测通过物理或天然过程释放的碱的存在的手段，校准光测量装置并确定存在于基础。

5. 发明授权

US5871938A Dioxetane compounds for the chemiluminescent detection of proteases, methods of use and kits therefore 失效
标题翻译：因此，用于化学发光检测蛋白酶的二氧杂环丁烷化合物，使用方法和试剂盒
公开(公告)号：US5871938A
公开(公告)日：1999-02-16
申请号：US935066
申请日：1997-09-22
申请人： Irena Bronstein , Brooks Edwards , Christopher Martin , Alison Sparks , John C. Voyta
发明人： Irena Bronstein , Brooks Edwards , Christopher Martin , Alison Sparks , John C. Voyta
IPC分类号： G01N33/573 , C07D321/00 , C07K5/072 , C07K5/08 , C07K5/09 , C07K5/10 , C12Q1/37 , G01N33/58 , C07K5/06
CPC分类号： C07K5/06095 , C07D321/00 , C07K5/0802 , C07K5/0817 , C07K5/1002 , C12Q1/37 , G01N33/582 , Y10S530/807
摘要： 1,2-dioxetane compounds bearing a proteolytic enzyme specific amino acid or peptide are provided, which amino acid or peptide can be removed by action of the corresponding protease. When the amino acid or peptide is removed, the 1,2-dioxetane decomposes with chemiluminescence, the generation of light providing a rapid, ultra-sensitive and convenient means for detecting the presence of the protease in the sample being inspected. The amount of light generated, or degree of chemiluminescence, can be correlated with the amount of protease present. Immunoassays, as well as DNA hybridization and DNA probe assays are provided.
摘要翻译：提供了带有蛋白水解酶特异性氨基酸或肽的1,2-二氧环乙烷化合物，该氨基酸或肽可以通过相应的蛋白酶的作用除去。当去除氨基酸或肽时，1,2-二氧杂环丁烷以化学发光分解，产生光，提供快速，超灵敏和方便的手段，用于检测待检样品中蛋白酶的存在。产生的光量或化学发光度可以与存在的蛋白酶的量相关。提供免疫测定，以及DNA杂交和DNA探针测定。

6. 发明授权

US6140495A Chemiluminescent 1,2-dioxetanes 失效
标题翻译：化学发光的1,2-二氧杂环丁烷
公开(公告)号：US6140495A
公开(公告)日：2000-10-31
申请号：US296539
申请日：1999-04-22
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： C07D321/00 , C07D327/06 , C07F9/02 , C07F9/06 , C07F9/28 , C07H19/04
CPC分类号： C07D321/00
摘要： Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T.sub.1/2 kinetics of the chemiluminescent reaction, as well as the signal intensity, or quantum yield of the chemiluminescent reaction, can be altered by selection of an electron-withdrawing or an electron-donating group Z, at positions on the aromatic substituent other than those adjacent the point of attachment to the dioxetane. Signal strength can further be enhanced by recognized chemiluminescent enhancers.
摘要翻译：具有烷氧基取代基的双金刚烷基二氧杂环丁烷和二氧杂环丁烷环上苯基或萘基的芳族取代基可以被活化成化学发光，如果芳族取代基具有指定为OX的部分，其中X被允许二氧杂环丁烷的酶切割在与接触。化学发光反应的T + E，1/2 + EE动力学以及化学发光反应的信号强度或量子产率可以通过选择吸电子基团或给电子基团Z来改变，在芳族取代基上除了与二氧杂环丁烷的连接点相邻的位置之外的位置。信号强度可以通过认可的化学发光增强剂进一步增强。

7. 发明授权

US6022964A Chemiluminescent 1,2-dioxetanes 失效
标题翻译：化学发光的1,2-二氧杂环丁烷
公开(公告)号：US6022964A
公开(公告)日：2000-02-08
申请号：US904847
申请日：1997-08-01
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： C07D321/00 , C07D327/06 , C07F9/02 , C07F9/06 , C07F9/28 , C07H19/04
CPC分类号： C07D321/00
摘要： Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T.sub.1/2 kinetics of the chemiluminescent reaction, as well as the signal intensity, or quantum yield of the chemiluminescent reaction, can be altered by selection of an electron-withdrawing or an electron-donating group Z, at positions on the aromatic substituent other than those adjacent the point of attachment to the dioxetane. Signal strength can further be enhanced by recognized chemiluminescent enhancers.
摘要翻译：具有烷氧基取代基的双金刚烷基二氧杂环丁烷和二氧杂环丁烷环上苯基或萘基的芳族取代基可以被活化成化学发光，如果芳族取代基具有指定为OX的部分，其中X被允许二氧杂环丁烷的酶切割在与接触。化学发光反应的T1 / 2动力学，以及化学发光反应的信号强度或量子产率可以通过选择吸电子基团或给电子基团Z来改变芳香族取代基上的位置比邻近二氧杂环丁烷的点附近的那些。信号强度可以通过认可的化学发光增强剂进一步增强。

8. 发明授权

US06346615B1 Chemiluminescent 1,2-dioxetanes 失效
标题翻译：化学发光的1,2-二氧杂环丁烷
公开(公告)号：US06346615B1
公开(公告)日：2002-02-12
申请号：US09656213
申请日：2000-09-06
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： C07H1904
CPC分类号： C07D321/00
摘要： Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T½ kinetics of the chemiluminescent reaction, as well as the signal intensity, or quantum yield of the chemiluminescent reaction, can be altered by selection of an electron-withdrawing or an electron-donating group Z, at positions on the aromatic substituent other than those adjacent the point of attachment to the dioxetane. Signal strength can further be enhanced by recognized chemiluminescent enhancers.
摘要翻译：具有烷氧基取代基的双金刚烷基二氧杂环丁烷和二氧杂环丁烷环上苯基或萘基的芳族取代基可以被活化成化学发光，如果芳族取代基具有指定为OX的部分，其中X被允许二氧杂环丁烷的酶切割在与接触。化学发光反应的T 1/2动力学，以及化学发光反应的信号强度或量子产率可以通过选择吸电子基团或给电子基团Z来改变芳香族取代基以外的芳香族取代基的位置邻近二氧杂环丁烷的连接点。信号强度可以通过认可的化学发光增强剂进一步增强。

9. 发明授权

US5869699A 1,2 chemiluminescent dioxetanes of improved performance 失效
公开(公告)号：US5869699A
公开(公告)日：1999-02-09
申请号：US871963
申请日：1997-06-10
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： C07D321/00 , C07F9/655 , C07G3/00 , C12Q1/37 , G01N33/58 , C07F9/28 , C07D305/14
CPC分类号： G01N33/582 , C07D321/00 , C07F9/65512 , C07G3/00 , C12Q1/37 , Y02P20/55
摘要： A new class of stable dioxetanes bears a polycyclic stabilizing group and aryloxy moiety, the oxygen atom of which is provided with a protective group which can be removed by an enzymatic or chemical trigger admixed with the dioxetane. The polycyclic stabilizing group is preferably spiroadamantane. The group further bears an alkoxy, aryloxy, aralkyloxy or cycloalkyloxy moiety which is partially or completely substituted with halogens, particularly fluorine and chlorine. Proper selection of electron active groups on the stabilizing moiety, the aryl group and the OR group yields enhanced enzyme kinetics, superior light intensity and excellent detection sensitivity in various assays.

10. 发明授权

US5851771A Chemiluminescent 1,2-dioxetanes 失效
公开(公告)号：US5851771A
公开(公告)日：1998-12-22
申请号：US588810
申请日：1996-01-19
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： G01N33/532 , A43B3/00 , C07C43/178 , C07C43/196 , C07C43/215 , C07C43/225 , C07C43/23 , C07C43/315 , C07C45/67 , C07C47/565 , C07C47/575 , C07C65/03 , C07C65/21 , C07C69/00 , C07C69/017 , C07C69/24 , C07C69/78 , C07C69/84 , C07C69/92 , C07C205/06 , C07C205/37 , C07C205/44 , C07C217/84 , C07C233/18 , C07C233/75 , C07C271/28 , C07C271/58 , C07C309/75 , C07C311/04 , C07C311/08 , C07C311/16 , C07D321/00 , C07F7/08 , C07F7/18 , C07F9/12 , C07F9/40 , C07F9/655 , C07F9/6574 , C07G3/00 , C07H15/203 , C07H15/26 , C12Q1/34 , C12Q1/37 , C12Q1/42 , C12Q1/68 , G01N21/76 , G01N21/78 , G01N33/52 , G01N33/535 , G01N33/58
CPC分类号： G01N33/582 , A43B1/0036 , A43B1/0072 , A43B3/0031 , C07C205/37 , C07C205/44 , C07C217/84 , C07C233/18 , C07C271/28 , C07C271/58 , C07C43/1788 , C07C43/196 , C07C43/215 , C07C43/225 , C07C43/23 , C07C43/315 , C07C45/673 , C07C47/565 , C07C47/575 , C07C65/03 , C07C65/21 , C07D321/00 , C07F9/12 , C07F9/4056 , C07F9/65512 , C07F9/65742 , C07G3/00 , C07H15/203 , C07H15/26 , C12Q1/34 , C12Q1/37 , C12Q1/42 , G01N21/76 , G01N33/581 , C07C2103/74 , C12Q2334/00 , Y10S435/81 , Y10S435/975
摘要： Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T.sub.1/2 kinetics of the chemiluminescent reaction, as well as the signal intensity, or quantum yield of the chemiluminescent reaction, can be altered by selection of an electron-withdrawing or an electron-donating group Z, at positions on the aromatic substituent other than those adjacent the point of attachment to the dioxetane. Signal strength can further be enhanced by recognized chemiluminescent enhancers.

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