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1. 发明申请

US20100105109A1 MULTIPLY-PRIMED AMPLIFICATION OF CIRCULAR NUCLEIC ACID SEQUENCES 审中-公开
标题翻译：圆形核酸序列的多重增殖放大
公开(公告)号：US20100105109A1
公开(公告)日：2010-04-29
申请号：US12532228
申请日：2008-03-18
申请人： Haiguang Xiao , John R. Nelson , Galina Chernaya , Haiying Wang , Paul Mitsis , Gyanendra Kumar , Carl F. Fuller , Yuyang Christine Cai
发明人： Haiguang Xiao , John R. Nelson , Galina Chernaya , Haiying Wang , Paul Mitsis , Gyanendra Kumar , Carl F. Fuller , Yuyang Christine Cai
IPC分类号： C12P19/34
CPC分类号： C12Q1/6806 , C12Q1/6844 , C12Q2531/125 , C12Q2521/319
摘要： Improved processes for the amplification of target DNA sequences in the form of single or double stranded circular DNA molecules, especially those present in colony and plaque extracts, using multiple specific and/or random sequence oligonucleotide primers are disclosed. The product of this amplification is used for analysis by restriction enzyme digestion or DNA sequencing and other analyses that involve hybridization. Kits containing components for use in the method are also described. Also described are further uses of this amplified DNA in sequencing, genotyping and haplotyping, and other molecular biology applications.
摘要翻译：公开了使用多个特异性和/或随机序列寡核苷酸引物扩增单链或双链环状DNA分子，特别是存在于菌落和斑块提取物中的靶DNA序列的改进方法。该扩增产物用于通过限制酶消化或DNA测序和涉及杂交的其他分析进行分析。还描述了包含用于该方法的组分的试剂盒。还描述了这种扩增的DNA在测序，基因分型和单元分型以及其他分子生物学应用中的进一步使用。

2. 发明申请

US20110172405A1 METHOD FOR SMALL RNA ISOLATION 审中-公开
标题翻译：小RNA分离方法
公开(公告)号：US20110172405A1
公开(公告)日：2011-07-14
申请号：US13063546
申请日：2009-09-17
申请人： Rohini Dhulipala , Yuyang Christine Cai , Miao Jiang , Mubasher Dar
发明人： Rohini Dhulipala , Yuyang Christine Cai , Miao Jiang , Mubasher Dar
IPC分类号： C07H21/02
CPC分类号： C12N15/111 , C12N15/1006 , C12N2310/141 , C12N2330/10
摘要： This invention relates to a simple and rapid method for the extraction and purification of small RNA from a sample solution. Accordingly, a sample is first mixed with an organic solvent to form a mixture containing the solvent. The mixture is applied to a first mineral support for large RNA to bind. The filtrate is collected which contain unbound small RNA, and is mixed with a second organic solvent to form a second mixture containing the second solvent. This second mixture is applied to a second mineral support for small RNA to bind. After a wash step, the small RNA is eluted. Also provided is a method for the isolation of large RNA, by eluting the large RNA from the first mineral support. In addition, total protein is present in the filtrate and can be isolated by a conventional method.
摘要翻译：本发明涉及从样品溶液中提取和纯化小RNA的简单而快速的方法。因此，首先将样品与有机溶剂混合以形成含有溶剂的混合物。将该混合物应用于第一种矿物载体以用于大RNA结合。收集含有未结合的小RNA的滤液，并与第二有机溶剂混合以形成含有第二溶剂的第二混合物。将该第二混合物应用于第二种矿物载体以用于小RNA结合。洗涤步骤后，小RNA被洗脱。还提供了通过从第一矿物质载体洗脱大RNA来分离大RNA的方法。此外，总蛋白存在于滤液中，可以通过常规方法分离。

3. 发明申请

US20090143570A1 METHOD FOR ISOLATION OF GENOMIC DNA, RNA AND PROTEINS FROM A SINGLE SAMPLE 审中-公开
标题翻译：从单个样品中分离基因DNA，RNA和蛋白质的方法
公开(公告)号：US20090143570A1
公开(公告)日：2009-06-04
申请号：US12277420
申请日：2008-11-25
申请人： Miao Jiang , Mark S. Briggs , Rohini Dhulipala , Yuyang Christine Cai , Renee E. Bruno
发明人： Miao Jiang , Mark S. Briggs , Rohini Dhulipala , Yuyang Christine Cai , Renee E. Bruno
IPC分类号： C07K1/16 , C07H21/04 , C07H21/02
CPC分类号： C12N15/1006 , C07K1/34
摘要： The invention provides systems, methods and kits for the separation and/or purification of at least two cellular components selected from genomic DNA, RNA and proteins. The method includes first lysing a biological sample to generate an aqueous solution containing the cellular components; then applying the aqueous solution to a first mineral support under conditions for genomic DNA to bind; and collecting the flowthrough which contains unbound total RNA and proteins. The method further includes applying the flowthrough to a second mineral support under conditions for RNA to bind, and collecting the flowthrough which contains proteins. The genomic DNA and total RNA bound can be eluted while the protein in the flowthrough can be further purified. Further the total RNA isolated could be used to isolate small RNA such as microRNA.
摘要翻译：本发明提供用于分离和/或纯化选自基因组DNA，RNA和蛋白质的至少两种细胞组分的系统，方法和试剂盒。该方法包括首先裂解生物样品以产生含有细胞组分的水溶液; 然后在将基因组DNA结合的条件下将该水溶液施加到第一矿物载体上; 并收集含有未结合的总RNA和蛋白质的流程。该方法还包括在RNA结合的条件下将流出物应用于第二矿物质载体，并收集含有蛋白质的流路。可以洗脱基因组DNA和结合的总RNA，同时可以进一步纯化流过的蛋白质。此外，分离的总RNA可用于分离小RNA如微小RNA。

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