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1. 发明专利

AU2002357533A1 METHOD OF SCREENING COMPOUND HAVING FUNGAL CELL WALL SYNTHESIS INHIBITORY ACTIVITY 未知
公开(公告)号：AU2002357533A1
公开(公告)日：2003-07-24
申请号：AU2002357533
申请日：2002-12-27
申请人： EISAI CO LTD
发明人： SATO TOSHITAKA , NAKAMOTO KAZUTAKA , TSUCHIYA MAMIKO , SAGANE KOJI , TSUKAHARA KAPPEI
IPC分类号： G01N33/50 , A61P31/10 , C07K14/40 , C12N15/09 , C12Q1/02 , C12Q1/18 , G01N33/15 , A61K45/00 , C07K14/195 , G01N33/566

2. 发明专利

JP2005168301A Method for screening compound having fungal cell wall synthesis-inhibiting activity 审中-公开
标题翻译：筛选具有真菌细胞壁合成抑制活性的化合物的方法
公开(公告)号：JP2005168301A
公开(公告)日：2005-06-30
申请号：JP2001401947
申请日：2001-12-28
申请人： Eisai Co Ltd , エーザイ株式会社
发明人： TSUKAHARA KATSUHEI , SATO TOSHITAKA , NAKAMOTO KAZUTAKA , TSUCHIYA MAMIKO
IPC分类号： G01N33/50 , A61P31/10 , C07K14/40 , C12N15/09 , C12Q1/02 , C12Q1/18 , G01N33/15
CPC分类号： C12Q1/18 , C07K14/40
摘要： PROBLEM TO BE SOLVED: To develop an antifungal agent which inhibits the transport of a GPI (glycorylphosphatidylinositol) anchor protein to a fungal cell wall to prevent the synthesis of the fungal cell wall and thereby inhibits the adhesion to a host cell to prevent the pathogenic fungus from exhibiting the pathogenicity. SOLUTION: A compound for inhibiting the transport of the GPI anchor protein to the fungal cell wall can be screened by a simple Binding assay using a membrane fraction expressing GWT1 protein (a GWT1 gene product). COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：开发一种抗真菌剂，其抑制GPI（糖基磷脂酰肌醇）锚蛋白转运至真菌细胞壁，以防止真菌细胞壁的合成，从而抑制对宿主细胞的粘附以防止致病真菌表现出致病性。解决方案：可以通过使用表达GWT1蛋白的膜级分（GWT1基因产物）的简单结合测定法筛选用于抑制GPI锚定蛋白转运到真菌细胞壁的化合物。版权所有（C）2005，JPO＆NCIPI

3. 发明专利

JP2000146954A METHOD FOR DISSOLUTION OF FIBRIN IN SERUM OR PLASMA SPECIMEN 失效
公开(公告)号：JP2000146954A
公开(公告)日：2000-05-26
申请号：JP24106199
申请日：1999-08-27
申请人： EISAI CO LTD
发明人： SATO TOSHITAKA , WATANABE KEISUKE , NARAKI TORU
IPC分类号： C12Q1/37 , C12Q1/48 , G01N33/48 , G01N33/543 , G01N33/553
摘要： PROBLEM TO BE SOLVED: To eliminate a measuring error generated when a solid substance existing in a specimen is mixed with the measuring system of a substance to be inspected, by a method wherein a method which dissolves a fibrinous substance in the specimen by using an enzyme is used, and a kit in which a reagent used to dissolve the fibrinous substance is used as a constituent component is used. SOLUTION: A buffer is diluted in such a way that the measured value of a substance to be inspected is not affected and that an enzyme can dissolve fibrin sufficiently. A phosphoric acid buffer at pH 7 or the like in which 1% bivine serum albumin and 0.15 M NaCl are contained is suitable. After that, a enzyme which dissolves fibrin, plasma or an additive which activates plasminogen in plasma and streptokinase are added. This mixture is incubated for a set time, the fibrin is dissolved, and the measurement of the substance to be measured is continued. The amount of the enzyme used for the dissolving reaction of the fibrin can be set at an amount by which the fibrin is dissolved sufficiently as long as a measured result is not affected substantially. That is to say, regarding a serum or plasma specimen, the streptokinase in different amount is added so as to be reacted.

4. 发明专利

JPH11124399A MONOCLONAL ANTIBODY FOR MEASURING PROTEIN C INHIBITOR-PROTEASE COMPLEX 失效
公开(公告)号：JPH11124399A
公开(公告)日：1999-05-11
申请号：JP28808697
申请日：1997-10-21
申请人： EISAI CO LTD
发明人： SATO TOSHITAKA , NARAKI TORU , SUZUKI KOJI
IPC分类号： G01N33/53 , C07K16/38 , C07K16/40 , C12N15/02 , C12P21/08 , G01N33/573 , G01N33/577
摘要： PROBLEM TO BE SOLVED: To obtain the subject new antibody comprising a monoclonal antibody capable of recognizing a protein C inhibitor-protease (activated protein C) complex (CIC) and useful for measurement or the like of the CIC without recognizing the intact protein C inhibitor. SOLUTION: This new monoclonal antibody is capable of recognizing a protein C inhibitor-protease (activated protein C) complex (CIC) without substantially recognizing the intact protein C inhibitor and further capable of recognizing the protein C inhibitor in the complex and is useful as a measuring reagent or the like capable of measuring the CIC useful as an index to know the state of blood coagulation and a fibrinolytic system. The antibody is obtained by administering the CIC prepared by reacting the activated protein C with the protein C inhibitor together with an adjuvant to a mouse peritoneal cavity, immunizing the mouse, then collecting a cell of the spleen, fusing the collected cell of the spleen to a cell of myeloma, selecting a strain capable of producing an anti-CIC antibody, monocloning the selected strain according to a limited dilution method and culturing the resultant monoclone.

5. 发明专利

JPH08220100A RHEUMATOID FACTOR MEASURING REAGENT 失效
公开(公告)号：JPH08220100A
公开(公告)日：1996-08-30
申请号：JP2572795
申请日：1995-02-14
申请人： EISAI CO LTD
发明人： SATO TOSHITAKA , FUJIMATSU JUNICHI , YOSHIZAWA MASAYUKI
IPC分类号： G01N33/53 , C07K16/00 , C07K16/18 , G01N33/564
摘要： PURPOSE: To perform the excellent determination without irregularity between lots by using one chain of IgGFc (Fc part of human immunity globulin) as the complementary antibody of rheumatoid factor. CONSTITUTION: One chain (Fc/2) of Fe part of IgG or its derivative is used as complementary antigen. The one chain can be used as it is, but it is desired that free SH group is, for example, protected by alkylating. Since the prepared IgGFe is more or less mixed with the Fe/2 according to the preparing conditions such as enzyme concentration and reaction time, if about 50% of the Fc/2 is included in the IgG or IgGFe which has heretofore been used, the object of certain degree can be performed. The more the ratio of the Fc/2 is increased, the greater the satisfactory result is given. The complementary antigen is desired to be solidified to solid phase from the view of the simplification of the operation.

6. 发明专利

JPH01161000A NEUTROPHIL-ACTIVATING FACTOR, ITS PRODUCTION AND ANTITUMOR AGENT AND ANTIBACTERIAL AGENT CONTAINING SAID FACTOR 失效
公开(公告)号：JPH01161000A
公开(公告)日：1989-06-23
申请号：JP31832887
申请日：1987-12-16
申请人： EISAI CO LTD
发明人： SHIONOYA HIROSHI , KOYANAGI NOZOMI , SATO TOSHITAKA , KUWATA MANABU , KOIDE JUN , MIYOSHI ISAO
IPC分类号： A61K38/00 , A61P1/04 , A61P31/04 , C07K1/20 , C07K14/00 , C07K14/52 , C12P21/00 , C12R1/91
摘要： NEW MATERIAL:A neutrophil-activating factor having a molecular weight of about 25,000 and containing a fragment having amino acid sequences of formula I-IV. USE:An antitumor agent and antibacterial agent. PREPARATION:An MT-2 cell which is an antigen-producing cell strain originated from human umbilical leukocyte is established by the mixed culture with leukemia cell collected from the peripheral blood of an adult T-cell leukemia patient. The MT-2 cell is cultured in a medium containing bovine fetus serum, etc., and the cultured liquid is centrifuged to precipitate and separate the cells and obtain the supernatant liquid. The supernatant liquid of the cultured product is passed through a molecular sieve membrane, concentrated, dialyzed, subjected to ion exchange column chromatography and eluted by the concentration gradient of sodium chloride. The eluted active fraction is purified by gel filtration and reversed phase high-performance liquid chromatography, etc., to obtain the objective neutrophil activating factor.

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