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2. 发明申请

US20060058249A1 Efficient nucleic acid encapsulation into medium sized liposomes 审中-公开
标题翻译：将有效的核酸封装到中等大小的脂质体中
公开(公告)号：US20060058249A1
公开(公告)日：2006-03-16
申请号：US10500933
申请日：2003-01-08
申请人： Shangguan Tong , Xingong Li , Paul Meers , Walter Perkins
发明人： Shangguan Tong , Xingong Li , Paul Meers , Walter Perkins
IPC分类号： A61K48/00 , A61K9/127
CPC分类号： A61K9/127 , A61K9/1277 , A61K47/36 , C12N15/88
摘要： A method for preparing liposomes containing at least one nucleic acid encapsulated therein comprising the following steps: (A) mixing a gel or a liquid containing gel particles with aqueous medium Z1 to directly form the liposomes containing the at least one nucleic acid encapsulated therein; (B)(i) mixing a gel or a liquid containing gel particles with aqueous medium Z1 to form a curd or curdy substance; and (ii) mixing the curd or curdy substance with aqueous medium Z2 to directly form the liposomes containing the at least one nucleic acid encapsulated therein; or (C)(i) cooling a gel or a liquid containing gel particles to form a waxy substance; and (ii) mixing the waxy substance with aqueous medium Z1 to directly form the liposomes containing the at least one nucleic acid encapsulated therein; wherein said gel or liquid containing gel particles comprises at least one liposome-forming lipid, at least one fusogenic lipid, a water-miscible organic solvent and the at least one nucleic acid; wherein an amount of the at least one fusogenic lipid is at least 20% by weight of a lipid content of the gel or the liquid containing gel particles; and wherein the aqueous media Z1 and Z2 are the same or different.
摘要翻译：一种制备含有至少一种包封的核酸的脂质体的方法，包括以下步骤：（A）将凝胶或含有凝胶颗粒的液体与水性介质Z1混合以直接形成包含至少一种核酸的脂质体; （B）（i）将凝胶或含有凝胶颗粒的液体与水性介质Z1混合以形成凝乳或卷曲物质; 和（ii）将凝乳或卷曲物质与水性介质Z2混合以直接形成含有其中包封的至少一种核酸的脂质体; 或（C）（i）冷却凝胶或含有凝胶颗粒的液体以形成蜡状物质; 和（ii）将蜡质物质与水性介质Z1混合以直接形成包含其中包含至少一种核酸的脂质体; 其中所述含凝胶或液体的凝胶颗粒包含至少一种形成脂质体的脂质，至少一种融合脂质，与水可混溶的有机溶剂和所述至少一种核酸; 其中所述至少一种融合脂质的量为所述凝胶或含有凝胶颗粒的液体的脂质含量的至少20重量％并且其中水性介质Z1和Z2相同或不同。

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