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1. 发明申请

US20140026523A1 PACKAGING SYSTEM AND METHOD 有权
标题翻译：包装系统和方法
公开(公告)号：US20140026523A1
公开(公告)日：2014-01-30
申请号：US13560068
申请日：2012-07-27
申请人： Nick Wilson , James Higley , Mark Burk
发明人： Nick Wilson , James Higley , Mark Burk
IPC分类号： B65B21/06 , F16B2/06 , B65D19/44
CPC分类号： B65B21/06 , B65B11/004 , B65B13/183 , B65B27/04 , B65B53/00 , B65B53/02 , B65D71/08
摘要： A packaging system and method forms a bundled group of articles in an oriented arrangement, by applying a wrapping material to the articles via the bottoms of the articles while the oriented arrangement of articles is inverted and retained by a pallet. The wrapping material may be a sleeve of shrinkable material applied to the inverted end of the articles and shrunk to conform to the articles and form a base enclosing the bottoms of the articles. The articles are retained by the pallet in the oriented arrangement during the inverting, sleeving and bundling of the group of articles, to provide a bundled group including the articles securely contained by the shrunk wrapping in the oriented arrangement. The bundled group may include more than one type of article. The pallet may be configured to retain a top portion of the article, which may have an irregular or asymmetrical shape.
摘要翻译：包装系统和方法通过在物品的定向布置被托盘反转和保持的同时通过物品的底部施加包裹材料到物品，形成定向布置的捆束组。包装材料可以是可收缩材料的套筒，其被施加到制品的倒置端并收缩以符合制品并形成包围制品底部的底座。在一组物品的翻转，套管和捆扎期间，物品被托盘保持在定向布置中，以提供包括被收缩包装物以固定方式安置的物品的捆扎组。捆绑组可以包括多于一种类型的物品。托盘可以构造成保持制品的顶部，其可以具有不规则或不对称的形状。

2. 发明申请

US20110306117A1 METHODS FOR ENZYMATIC DECOLORIZATION OF CHLOROPHYLL 有权
标题翻译：氯化氢酶促脱酸方法
公开(公告)号：US20110306117A1
公开(公告)日：2011-12-15
申请号：US13103276
申请日：2011-05-09
申请人： DAVID LAM , DAVID WEINER , TIMOTHY HITCHMAN , NELSON BARTON , MARK BURK
发明人： DAVID LAM , DAVID WEINER , TIMOTHY HITCHMAN , NELSON BARTON , MARK BURK
IPC分类号： C12S3/00
CPC分类号： C11B3/003 , C12N9/18
摘要： The invention provides the invention provides compositions and methods for the enzymatic treatment (“bleaching” or “de-colorizing”) of chlorophyll-comprising compositions, e.g., algae preparations, chlorophyll-containing or chlorophyll-contaminated feeds, foods or oils, for example, vegetable oils, including oils processed from oilseeds, such as canola (rapeseed) oil or soybean oil, or oil fruits, such as palm oil. In one aspect, the invention provides methods using a chlorophyllase enzyme for the enzymatic hydrolysis of chlorophyll in an algae, an animal (e.g., a fish) or plant preparation, a food or an oil. In one aspect, the chlorophyllase is immobilized onto a silica. The invention also provides compositions of manufacture and detergents.
摘要翻译：本发明提供了本发明提供了含叶绿素组合物的酶处理（“漂白”或“脱色”）的组合物和方法，例如藻类制剂，含叶绿素或叶绿素污染的饲料，食品或油，例如植物油，包括从油籽加工的油，例如油菜籽油菜籽油或大豆油，或油果如棕榈油。一方面，本发明提供了使用叶绿素酶在藻类，动物（例如鱼）或植物制剂，食品或油中进行叶绿素的酶水解的方法。一方面，将叶绿素酶固定在二氧化硅上。本发明还提供了制造和洗涤剂的组合物。

3. 发明申请

US20110201071A1 Methods and organisms for the growth-coupled production of 1,4-Butanediol 有权
标题翻译：用于1,4-丁二醇生长偶联生产的方法和生物
公开(公告)号：US20110201071A1
公开(公告)日：2011-08-18
申请号：US13065303
申请日：2011-03-18
申请人： Anthony P. Burgard , Stephen J. Van Dien , Mark Burk
发明人： Anthony P. Burgard , Stephen J. Van Dien , Mark Burk
IPC分类号： C12P7/18 , C12N1/00
CPC分类号： C12P7/18 , B01D3/002 , C12N9/0006
摘要： The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby theone or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non-naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4-butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO.
摘要翻译：本发明提供了包含一种或多种基因破坏的非天然存在的微生物，所述一个或多个基因破坏发生在编码需要将1,4-丁二醇生产偶联以生产微生物的酶的基因发生在基因破坏时降低活性该酶，其中一种或多种基因破坏赋予非天然存在的微生物上1,4-丁二醇稳定的生长偶联产生。微生物还可以包含编码1,4-丁二醇（BDO）生物合成途径中的酶的基因。本发明还涉及使用微生物来生产BDO的方法。

4. 发明申请

US20080182303A1 Methods for Making Simvastatin and Intermediates 失效
标题翻译：辛伐他汀和中间体的制备方法
公开(公告)号：US20080182303A1
公开(公告)日：2008-07-31
申请号：US10576122
申请日：2004-10-20
申请人： Brian Morgan , Mark Burk , Michael Levin , Zuolin Zhu , Jennifer Chaplin , Karen Kustedjo , Zillin Huang , William Greenberg
发明人： Brian Morgan , Mark Burk , Michael Levin , Zuolin Zhu , Jennifer Chaplin , Karen Kustedjo , Zillin Huang , William Greenberg
IPC分类号： C12P17/06 , C12P17/02 , C12P7/18
CPC分类号： C12P17/06 , C07D309/30
摘要： The invention provides synthetic chemical and chemoenzymatic methods of producing simvastatin and various intermediates. In one aspect, enzymes such as hydrolases, e.g., esterases, are used in the methods of the invention.
摘要翻译：本发明提供了生产辛伐他汀和各种中间体的合成化学和化学酶学方法。在一个方面，酶如水解酶，例如酯酶，用于本发明的方法中。

5. 发明申请

US20070231820A1 P450 ENZYMES, NUCLEIC ACIDS ENCODING THEM AND METHODS OF MAKING AND USING THEM 审中-公开
标题翻译： P450酶，核酸编码和制备和使用它们的方法
公开(公告)号：US20070231820A1
公开(公告)日：2007-10-04
申请号：US11687606
申请日：2007-03-16
申请人： David Weiner , Mark Burk , Tim Hitchman , Catherine Pujol , Toby Richardson , Jay Short
发明人： David Weiner , Mark Burk , Tim Hitchman , Catherine Pujol , Toby Richardson , Jay Short
IPC分类号： C12Q1/68 , A01H5/00 , A01K67/027 , C07H21/04 , C07H5/04 , C07K1/02 , C07K1/04 , C07K1/06 , C07K1/107 , C07K1/12 , C07K16/40 , C12N1/08 , C12N1/15 , C12N1/19 , C12N1/21 , C12N15/63 , C12N5/10 , C12N5/12 , G06F17/30 , G06F17/00 , G01N33/573 , C12P13/02 , C12P21/06 , C12P5/00 , C12P7/02 , C12Q1/26
CPC分类号： C12N9/0071
摘要： The invention is directed to polypeptides having P450 activity, polynucleotides encoding the polypeptides, antibodies that bind to these polypeptides, and methods for making and using these polynucleotides and polypeptides. The P450 enzymes can be used to catalyze the hydrolysis of epoxides and arene oxides to their corresponding diols.
摘要翻译：本发明涉及具有P450活性的多肽，编码多肽的多核苷酸，与这些多肽结合的抗体，以及制备和使用这些多核苷酸和多肽的方法。 P450酶可用于催化环氧化物和芳烃氧化物水解成其相应的二醇。

6. 发明授权

US09260213B2 Packaging system 有权
标题翻译：包装系统
公开(公告)号：US09260213B2
公开(公告)日：2016-02-16
申请号：US13560068
申请日：2012-07-27
申请人： Nick Wilson , James Higley , Mark Burk
发明人： Nick Wilson , James Higley , Mark Burk
IPC分类号： B65B13/00 , B65B53/02 , B65B27/04 , B65D71/08
CPC分类号： B65B21/06 , B65B11/004 , B65B13/183 , B65B27/04 , B65B53/00 , B65B53/02 , B65D71/08
摘要： A packaging system and method forms a bundled group of articles in an oriented arrangement, by applying a wrapping material to the articles via the bottoms of the articles while the oriented arrangement of articles is inverted and retained by a pallet. The wrapping material may be a sleeve of shrinkable material applied to the inverted end of the articles and shrunk to conform to the articles and form a base enclosing the bottoms of the articles. The articles are retained by the pallet in the oriented arrangement during the inverting, sleeving and bundling of the group of articles, to provide a bundled group including the articles securely contained by the shrunk wrapping in the oriented arrangement. The bundled group may include more than one type of article. The pallet may be configured to retain a top portion of the article, which may have an irregular or asymmetrical shape.
摘要翻译：包装系统和方法通过在物品的定向布置被托盘反转和保持的同时通过物品的底部施加包裹材料到物品，形成定向布置的捆束组。包装材料可以是可收缩材料的套筒，其被施加到制品的倒置端并收缩以符合制品并形成包围制品底部的底座。在一组物品的翻转，套管和捆扎期间，物品被托盘保持在定向布置中，以提供包括被收缩包装物以固定方式安置的物品的捆扎组。捆绑组可以包括多于一种类型的物品。托盘可以构造成保持制品的顶部，其可以具有不规则或不对称的形状。

7. 发明授权

US09017990B2 Methods for enzymatic decolorization of chlorophyll 有权
标题翻译：叶绿素酶脱色方法
公开(公告)号：US09017990B2
公开(公告)日：2015-04-28
申请号：US13103276
申请日：2011-05-09
申请人： David Lam , David Weiner , Timothy Hitchman , Nelson Barton , Mark Burk
发明人： David Lam , David Weiner , Timothy Hitchman , Nelson Barton , Mark Burk
IPC分类号： C12P7/64 , C12S3/00 , C12S3/18 , C12N9/18
CPC分类号： C11B3/003 , C12N9/18
摘要： The invention provides the invention provides compositions and methods for the enzymatic treatment (“bleaching” or “de-colorizing”) of chlorophyll-comprising compositions, e.g., algae preparations, chlorophyll-containing or chlorophyll-contaminated feeds, foods or oils, for example, vegetable oils, including oils processed from oilseeds, such as canola (rapeseed) oil or soybean oil, or oil fruits, such as palm oil. In one aspect, the invention provides methods using a chlorophyllase enzyme for the enzymatic hydrolysis of chlorophyll in an algae, an animal (e.g., a fish) or plant preparation, a food or an oil. In one aspect, the chlorophyllase is immobilized onto a silica. The invention also provides compositions of manufacture and detergents.
摘要翻译：本发明提供了本发明提供了含叶绿素组合物的酶处理（“漂白”或“脱色”）的组合物和方法，例如藻类制剂，含叶绿素或叶绿素污染的饲料，食品或油，例如植物油，包括从油籽加工的油，例如油菜籽油菜籽油或大豆油，或油果如棕榈油。一方面，本发明提供了使用叶绿素酶在藻类，动物（例如鱼）或植物制剂，食品或油中进行叶绿素的酶水解的方法。一方面，将叶绿素酶固定在二氧化硅上。本发明还提供了制造和洗涤剂的组合物。

8. 发明授权

US07700329B2 Methods for making simvastatin and intermediates 失效
标题翻译：辛伐他汀和中间体的制备方法
公开(公告)号：US07700329B2
公开(公告)日：2010-04-20
申请号：US10576122
申请日：2004-10-20
申请人： Brian Morgan , Mark Burk , Michael Levin , Zuolin Zhu , Jennifer Chaplin , Karen Kustedjo , Zilin Huang , William Greenberg
发明人： Brian Morgan , Mark Burk , Michael Levin , Zuolin Zhu , Jennifer Chaplin , Karen Kustedjo , Zilin Huang , William Greenberg
IPC分类号： C12P17/06 , C12P17/12 , C12P17/18
CPC分类号： C12P17/06 , C07D309/30
摘要： The invention provides synthetic chemical and chemoenzymatic methods of producing simvastatin and various intermediates. In one aspect, enzymes such as hydrolases, e.g., esterases, are used in the methods of the invention.
摘要翻译：本发明提供了生产辛伐他汀和各种中间体的合成化学和化学酶学方法。在一个方面，酶如水解酶，例如酯酶，用于本发明的方法中。

9. 发明申请

US20090047719A1 Methods and organisms for the growth-coupled production of 1,4-butanediol 有权
标题翻译：用于1,4-丁二醇生长偶联生产的方法和生物
公开(公告)号：US20090047719A1
公开(公告)日：2009-02-19
申请号：US11891602
申请日：2007-08-10
申请人： Anthony P. Burgard , Stephen J. Van Dien , Mark Burk
发明人： Anthony P. Burgard , Stephen J. Van Dien , Mark Burk
IPC分类号： C12P7/18 , C12N1/00 , C12N1/20 , C12N1/16 , C12N1/14
CPC分类号： C12P7/18 , B01D3/002 , C12N9/0006
摘要： The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby theone or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non-naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4-butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO.
摘要翻译：本发明提供了包含一种或多种基因破坏的非天然存在的微生物，所述一个或多个基因破坏发生在编码需要将1,4-丁二醇生产偶联以生产微生物的酶的基因发生在基因破坏时降低活性该酶，其中一种或多种基因破坏赋予非天然存在的微生物上1,4-丁二醇稳定的生长偶联产生。微生物还可以包含编码1,4-丁二醇（BDO）生物合成途径中的酶的基因。本发明还涉及使用微生物来生产BDO的方法。

10. 发明申请

US20070015260A1 Synthesis of synthons for the manufacture of bioactive compounds 审中-公开
标题翻译：用于生产生物活性化合物的合成子的合成
公开(公告)号：US20070015260A1
公开(公告)日：2007-01-18
申请号：US11481653
申请日：2006-07-05
申请人： Chi-Huey Wong , Junjie Liu , Grace DeSantis , Mark Burk
发明人： Chi-Huey Wong , Junjie Liu , Grace DeSantis , Mark Burk
IPC分类号： C08B37/00 , C12P19/02 , C12N9/24
CPC分类号： C12P19/02 , C12N9/88 , C12P17/06 , C12R1/19
摘要： The present invention is based on the discovery that 2-deoxyribose-5-phosphate aldolase (DERA, EC 4.1.2.4) and variants thereof can be used to catalyze sequential asymmetric aldol reactions between a wide variety of donor and acceptor aldehydes. The reaction products typically contain at least two new stereogenic centers and can be produced in enantiomerically pure form. As such, DERA catalyzed asymmetric aldol chemistry can be exploited to produce synthons for the synthesis of a variety of bioactive molecules.
摘要翻译：本发明基于以下发现：2-脱氧核糖-5-磷酸醛缩酶（DERA，EC 4.1.2.4）及其变体可用于催化多种供体和受体醛之间的顺序不对称醛醇反应。反应产物通常含有至少两个新的立体中心并且可以以对映体纯的形式制备。因此，DERA催化的不对称醛醇化学可以被利用来产生用于合成各种生物活性分子的合成物。

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