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    • 10. 发明公开
    • NUKLEINS[URE-BIDENDE CHIPS ZUR DETEKTION VON PHOSPHATMANGELZUST[NDEN IM RAHMEN DER BIOPROZESSKONTROLLE
    • NUKLEINS [URE-BIDENDE CHIPS ZUR DETEKTION VON PHOSPHATMANGELZUST [NDEN IM RAHMEN DER BIOPROZESSKONTROLLE
    • EP1828416A1
    • 2007-09-05
    • EP05822552.5
    • 2005-12-15
    • HENKEL KOMMANDITGESELLSCHAFT AUF AKTIENErnst-Moritz-Arndt-Universität Greifswald
    • SCHWEDER, ThomasJÜRGEN, BrittaEVERS, StefanMAURER, Karl-HeinzHOI, Le, ThiHECKER, MichaelVOIGT, BirgitFEESCHE, Jörg
    • C12Q1/68
    • C12Q1/689
    • The invention relates to nucleic acid-binding chips for monitoring bioprocesses, especially for detecting phosphate deficiency conditions. Said chips support probes for at least three of the following 47 genes: yhcR, tatCD, ctaC, gene for a putative acetoin reductase, spollGA, nasE, pstA, spollAA, gene for a hypothetical protein, yhbD, cotE, gene for a conserved hypothetical protein, yurl, spoVID, gene for a putative aromatics-specific dioxygenase, yhbE, gene for a putative benzoate transport protein, pstBB, spoIIIAH, gene for a hypothetical protein, spollQ, spollIAG, yvmA, gene for a putative ribonuclease, dhaS, yrbE, gene for a putative decarboxylase/dehydratase, htpG, yfkH, spollAB, spolllAF, alsD, gdh, yfkN, pstC, yfmQ, pstBA, dhaS homologue, gene for a putative phosphatase, phy, cypX, alsS, phoD, pstS, phoB, yvnA, yvmC, the total number of phosphate metabolism-specific different probes on the nucleic acid-binding chips not exceeding 100. The invention further relates to the use of corresponding gene probes, particularly on such chips, as well as corresponding methods and possible uses.
    • 本发明涉及用于监测生物过程特别是用于检测磷酸盐缺陷症状的核酸结合芯片。 所述芯片支持针对以下47个基因中的至少三个的探针:用于假定的乙偶姻还原酶的基因yhcR,tatCD,ctaC,假想蛋白质的基因yspb,spollGA,假基因yhbD,cotE基因,用于保守假设的基因 蛋白质,yurl,spoVID,推定的芳香族特异性双加氧酶基因,yhbE,推定的苯甲酸转运蛋白基因,pstBB,spoIIIAH,假定蛋白质的基因spollQ,spollIAG,yvmA,推定的核糖核酸酶基因dhaS,yrbE ,推定的磷酸酶基因,phy,cypX,alsS,phoD,pstS,phoB,htpG,yfkH,spollAB,spolIIAF,alsD,gdh,yfkN,pstC,yfmQ,pstBA,dhaS同源物, yvnA,yvmC,核酸结合片上磷酸代谢特异性不同探针的总数不超过100.本发明进一步涉及相应基因探针的用途,特别是在这种芯片上的用途,以及相应的方法和可能的用途 。