发明申请
WO2021081194A8 MEASUREMENT OF MOLECULAR INTERACTIONS AT SINGLE MOLECULE LEVEL USING SUBSTRATES THAT ENHANCE FLUORESCENCE DETECTION
审中-公开
基本信息:
- 专利标题： MEASUREMENT OF MOLECULAR INTERACTIONS AT SINGLE MOLECULE LEVEL USING SUBSTRATES THAT ENHANCE FLUORESCENCE DETECTION
- 申请号：PCT/US2020/056849 申请日：2020-10-22
- 公开(公告)号：WO2021081194A8 公开(公告)日：2021-04-29
- 发明人： THRUSH, Evan , LIU, Ning , MARLOWE, Carl , STRONG, William, Bradley
- 申请人： BIO-RAD LABORATORIES, INC.
- 申请人地址： 1000 Alfred Nobel Drive
- 专利权人： BIO-RAD LABORATORIES, INC.
- 当前专利权人： BIO-RAD LABORATORIES, INC.
- 当前专利权人地址： 1000 Alfred Nobel Drive
- 代理机构： EISENSCHENK, Frank C. et al.
- 优先权： US62/924,337 2019-10-22
- 主分类号： G01N33/52
- IPC分类号： G01N33/52 ; G01N33/53 ; G01N21/553 ; G01N21/6458 ; G01N21/648 ; G01N33/68 ; G06T2207/30024 ; G06T7/0014
摘要：
The invention relates to methods for characterizing the binding interactions between binding partners. The invention pertains to methods comprising contacting a first binding partner with a second binding partner that exhibits fast-off rate binding characteristics with the first binding partner to generate the binding interaction between the first binding partner and the second binding partner, wherein the first binding partner is immobilized onto a substrate that is designed to enhance the fluorescence signal of fluorescent molecules located near the substrate's surface, and wherein the second binding partner is a molecule that emits a fluorescent signal or is conjugated to molecule that emits a fluorescent signal. The interactions between the two binding partners can be analyzed based on multiple transient interactions between the two binding partners.