基本信息:
- 专利标题： METHODS AND SYSTEMS FOR CONCENTRATION OF SAMPLES FOR LATERAL FLOW ASSAYS
- 申请号：PCT/US2019/036705 申请日：2019-06-12
- 公开(公告)号：WO2019241342A1 公开(公告)日：2019-12-19
- 发明人： CONNELLY, John Thomas , DANTZLER, Jeffrey L. , GLUKHOVA, Veronika A. , GRANT, Benjamin David , HARSTON, Stephen Paul , IVEZIC, Vedrana , WEIGL, Bernhard Hans , YILDIRIM, Ozgur Emek
- 申请人： TOKITAE LLC
- 申请人地址： 3150 139th Ave SE Bellevue, Washington 98005-4046 US
- 专利权人： TOKITAE LLC
- 当前专利权人： TOKITAE LLC
- 当前专利权人地址： 3150 139th Ave SE Bellevue, Washington 98005-4046 US
- 代理机构： SCHUBERT, Elizabeth L. et al.
- 优先权： US16/006,984 20180613
- 主分类号： G01N33/543
- IPC分类号： G01N33/543 ; G01N1/40 ; B01D15/34 ; B01L3/00
摘要：
Methods and systems for capture concentration of analytes using lectins and other capture ligands are described. For example, stationary phase media functionalized with lectins are used for capture concentration and cleaning of TB lipoarabinomannan (TB LAM) prior to assay on a lateral flow assay (LFA) device, and filtration devices suitable for particulate or bulk capture media are described. Size-exclusion filtration is used to separate particles with captured analyte during washing and concentration steps. Captured analyte can be eluted from stationary phase media prior to application to a LFA or eluted directly onto a customized LFA device that includes a size-selective filter. In various aspects, a size-selective filter on a LFA is used to transfer particulate capture media on a LFA device.
IPC结构图谱:
| G | 物理 |
| --G01 | 测量；测试 |
| ----G01N | 借助于测定材料的化学或物理性质来测试或分析材料 |
| ------G01N33/00 | 利用不包括在G01N1/00至G01N31/00组中的特殊方法来研究或分析材料 |
| --------G01N33/48 | .生物物质，例如血、尿（G01N33/02至G01N33/14，G01N33/26，G01N33/44，G01N33/46优先；种子发芽能力测定入A01C1/02）；血球计数器 |
| ----------G01N33/483 | ..生物物质的物理分析 |
| ------------G01N33/53 | ...免疫测定法；生物特有的结合方法的测定；相应的生物物质 |
| --------------G01N33/543 | ....用不溶解的载体固定免疫化学物品 |