基本信息:
- 专利标题： OLEAGINOUS MICROALGAE HAVING AN LPAAT ABLATION
- 专利标题（中）：具有LPAAT消融的OLEAGINOUS MICROALGAE
- 申请号：PCT/US2016/026265 申请日：2016-04-06
- 公开(公告)号：WO2016164495A1 公开(公告)日：2016-10-13
- 发明人： FRANKLIN, Scott , BHAT, Riyaz , ZHAO, Xinhua
- 申请人： SOLAZYME, INC.
- 申请人地址： 225 Gateway Boulevard South San Francisco, California 94080 US
- 专利权人： SOLAZYME, INC.
- 当前专利权人： SOLAZYME, INC.
- 当前专利权人地址： 225 Gateway Boulevard South San Francisco, California 94080 US
- 代理机构： SUH, Gerald J et al.
- 优先权： US62/143,711 20150406; US62/145,723 20150410
- 主分类号： C12P7/64
- IPC分类号： C12P7/64 ; C12N1/13
摘要：
Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, lysophosphatidic acid acyltransferase, ketoacyl-CoA reductase, hydroxyacyl-CoA dehydratase, and/or enoyl-CoA reductase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
摘要（中）：
重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP去饱和酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶，溶血磷脂酰基转移酶，酮酰基-CoA还原酶，羟基酰基-CoA脱水酶和/或烯酰辅酶A还原酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集中链型，或者油可富含饱和不饱和饱和型甘油三酸酯。
IPC结构图谱:
| C | 化学；冶金 |
| --C12 | 生物化学；啤酒；烈性酒；果汁酒；醋；微生物学；酶学；突变或遗传工程 |
| ----C12P | 发酵或使用酶的方法合成目标化合物或组合物或从外消旋混合物中分离旋光异构体 |
| ------C12P7/00 | 含氧有机化合物的制备 |
| --------C12P7/64 | .脂肪；脂油；酯型蜡；高级脂肪酸，即在一连接羧基的完整的链上至少有7个碳原子；氧化油或脂 |