基本信息:
- 专利标题： HYBRID PORTABLE ORIGIN OF REPLICATION PLASMIDS
- 专利标题（中）：复制流体的混合原产地
- 申请号：PCT/US2006/035433 申请日：2006-09-13
- 公开(公告)号：WO2007035323A1 公开(公告)日：2007-03-29
- 发明人： BOGOSIAN, Gregg , O'NIEL, Julia, P. , SMITH, Hong, Q.
- 申请人： MONSANTO TECHNOLOGY LLC , BOGOSIAN, Gregg , O'NIEL, Julia, P. , SMITH, Hong, Q.
- 申请人地址： 800 North Lindbergh Boulevard, St. Louis, MO 63167 US
- 专利权人： MONSANTO TECHNOLOGY LLC,BOGOSIAN, Gregg,O'NIEL, Julia, P.,SMITH, Hong, Q.
- 当前专利权人： MONSANTO TECHNOLOGY LLC,BOGOSIAN, Gregg,O'NIEL, Julia, P.,SMITH, Hong, Q.
- 当前专利权人地址： 800 North Lindbergh Boulevard, St. Louis, MO 63167 US
- 代理机构： KAMMERER, Patricia, A.
- 优先权： US60/718,083 20050916
- 主分类号： C12N15/69
- IPC分类号： C12N15/69
摘要：
The invention relates to modifying plasmid origins of replication to create hybrid origins of replication containing nucleotide sequences from more than one plasmid. The invention also relates to a modified origin of replication cassette that is portable or exchangeable due to the creation of multiple cloning sites flanking the origin of replication. Methods and plasmids for use in exchanging origins of replication are disclosed. Such modified or hybrid plasmids provide useful cloning tools that allow for regulation of the level of expression of a desired protein.
摘要（中）：
本发明涉及修饰质粒复制起点以产生含有来自多于一种质粒的核苷酸序列的复制复制起点。 本发明还涉及由于产生位于复制起点两侧的多个克隆位点而可移植或可交换的复制盒的修饰起点。 公开了用于交换复制起点的方法和质粒。 这样的修饰或杂交质粒提供了有用的克隆工具，其允许调节所需蛋白质的表达水平。