基本信息:
- 专利标题： PERFUSED THREE-DIMENSIONAL CELL/TISSUE DISEASE MODELS
- 专利标题（中）：丰富的三维细胞/组织疾病模型
- 申请号：PCT/US2005/017594 申请日：2005-05-19
- 公开(公告)号：WO2005123950A2 公开(公告)日：2005-12-29
- 发明人： DOMANSKY, Karel , GRIFFITH, Linda, G. , TANNENBAUM, Steven, R. , WELLS, Alan
- 申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , UNIVERSITY OF PITTSBURGH , DOMANSKY, Karel , GRIFFITH, Linda, G. , TANNENBAUM, Steven, R. , WELLS, Alan
- 申请人地址： 77 Massachusetts Avenue, Cambridge, MA 02139 US
- 专利权人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY,UNIVERSITY OF PITTSBURGH,DOMANSKY, Karel,GRIFFITH, Linda, G.,TANNENBAUM, Steven, R.,WELLS, Alan
- 当前专利权人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY,UNIVERSITY OF PITTSBURGH,DOMANSKY, Karel,GRIFFITH, Linda, G.,TANNENBAUM, Steven, R.,WELLS, Alan
- 当前专利权人地址： 77 Massachusetts Avenue, Cambridge, MA 02139 US
- 代理机构： PABST, Patrea, L.
- 优先权： US60/572,583 20040519
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
A system has been constructed that recapitulate the features of a capillary bed through normal human tissue. The system facilitates perfusion of three-dimensional (3D) cell monocultures and heterotypic cell co-cultures at the length scale of the capillary bed. A major feature is that the system can be utilized within a "multiwell plate" format amenable to highthroughput assays compatible with the type of robotics commonly used in pharmaceutical development. The system provides a means to conduct assays for toxicology and metabolism and as a model for human diseases such as hepatic diseases, including hepatitis, exposure-related pathologies, and cancer. Cancer applications include primary liver cancer as well as metastases. The system can also be used as a means of testing gene therapy approaches for treating disease and inborn genetic defects.
摘要（中）：
已经构建了通过正常人体组织重现毛细血管床特征的系统。 该系统有助于在毛细血管床的长度尺度上灌注三维（3D）细胞单一培养物和异型细胞共培养物。 一个主要特征是该系统可以在“多孔板”格式中使用，适合与通常用于药物开发的机器人类型相容的高通量测定。 该系统提供了进行毒理学和代谢测定的手段，并且作为人类疾病（如肝脏疾病，包括肝炎，暴露相关病理和癌症）的模型。 癌症应用包括原发性肝癌以及转移瘤。 该系统还可用作测试基因治疗方法的手段，用于治疗疾病和先天性遗传缺陷。