基本信息:
- 专利标题： APPARATUS FOR GENERATING ELECTRICAL PULSES AND METHODS OF USING THE SAME
- 专利标题（中）：用于产生电脉冲的装置及其使用方法
- 申请号：PCT/US2004/023078 申请日：2004-07-19
- 公开(公告)号：WO2005032646A2 公开(公告)日：2005-04-14
- 发明人： BEEBE, Stephen, J. , SCHOENBACH, Karl, H.
- 申请人： EASTERN VIRGINIA MEDICAL SCHOOL , OLD DOMINION UNIVERSITY , BEEBE, Stephen, J. , SCHOENBACH, Karl, H.
- 申请人地址： P.O. Box 1980, Norfolk, VA 23501-1980 US
- 专利权人： EASTERN VIRGINIA MEDICAL SCHOOL,OLD DOMINION UNIVERSITY,BEEBE, Stephen, J.,SCHOENBACH, Karl, H.
- 当前专利权人： EASTERN VIRGINIA MEDICAL SCHOOL,OLD DOMINION UNIVERSITY,BEEBE, Stephen, J.,SCHOENBACH, Karl, H.
- 当前专利权人地址： P.O. Box 1980, Norfolk, VA 23501-1980 US
- 代理机构： THENOR, Leonid, D.
- 优先权： US60/487,932 200307018; US60/499,921 20030904; US60/526,585 20031204
- 主分类号： A61N
- IPC分类号： A61N
摘要：
A method and apparatus are provided for delivering an agent into a cell through the application of nanosecond pulse electric fields ("nsPEF's"). The method includes circuitry for delivery of an agent into a cell via known methods followed by the application of nanosecond pulse electric fields to said cell in order to facilitate entry of the agent into the nucleus of the cell. In a preferred embodiment, the present invention is directed to a method of enhancing gene expression in a cell comprising the application of nanosecond pulse electric fields to said cell. An apparatus for generating long and short pulses according to the present invention is also provided. The apparatus includes a pulse generator capable of producing a first pulse having a long duration and low voltage amplitude and a second pulse having a short duration and high voltage amplitude.
摘要（中）：
提供了一种方法和装置，用于通过施加纳秒脉冲电场（“nsPEF”）将试剂输送到电池中。 该方法包括用于通过已知方法将试剂递送到细胞中的电路，随后将纳秒脉冲电场施加到所述细胞，以便促进试剂进入细胞核。 在优选的实施方案中，本发明涉及增强细胞中基因表达的方法，其包括向所述细胞施加纳秒脉冲电场。 还提供了根据本发明的用于产生长脉冲和短脉冲的装置。 该装置包括能够产生具有长持续时间和低电压振幅的第一脉冲和具有短持续时间和高电压振幅的第二脉冲的脉冲发生器。
IPC结构图谱:
| A | 人类生活必需 |
| --A61 | 医学或兽医学；卫生学 |
| ----A61N | 电疗；磁疗；放射疗；超声波疗 |