基本信息:
- 专利标题： IN VITRO TRANSLATION SYSTEM
- 专利标题（中）：在体外翻译系统
- 申请号：PCT/US2004013110 申请日：2004-04-27
- 公开(公告)号：WO2004096999A2 公开(公告)日：2004-11-11
- 发明人： LEE JAE MOON , BUCKLEY DOUGLAS IWEN , CANCILLA MICHAEL ROBERT , CURTIS DAMIAN E , BOWMAN KRISTA K , ZHAN HANGJUN , CIANCIO MARGIE
- 申请人： EXELIXIS INC , LEE JAE MOON , BUCKLEY DOUGLAS IWEN , CANCILLA MICHAEL ROBERT , CURTIS DAMIAN E , BOWMAN KRISTA K , ZHAN HANGJUN , CIANCIO MARGIE
- 专利权人： EXELIXIS INC,LEE JAE MOON,BUCKLEY DOUGLAS IWEN,CANCILLA MICHAEL ROBERT,CURTIS DAMIAN E,BOWMAN KRISTA K,ZHAN HANGJUN,CIANCIO MARGIE
- 当前专利权人： EXELIXIS INC,LEE JAE MOON,BUCKLEY DOUGLAS IWEN,CANCILLA MICHAEL ROBERT,CURTIS DAMIAN E,BOWMAN KRISTA K,ZHAN HANGJUN,CIANCIO MARGIE
- 优先权： US46596303 2003-04-28
- 主分类号： C12N
- IPC分类号： C12N20060101 ; C12N15/67 ; C12P21/00 ; C12P21/02 ; C12Q1/68 ; G01N33/53 ; C12N
摘要：
In vitro translation (IVT) systems and methods for increased expression of proteins from linear templates, using GamS, are provided. The proteins may be full length or protein fragments. The IVT system may be used in batch or continuous mode. The GamS may be used as GamS nucleic acid template, crude protein fraction, or purified protein product. The IVT system using GamS component may be employed in a high-throughput mode. The ability to predict expressible protein or fragments, and activity and solubility of a large-scale protein expression product based on the results obtained from high-throughput, small-scale IVT expression product is also provided.
摘要（中）：
提供了体外翻译（IVT）系统和使用GamS从线性模板中增加蛋白质表达的方法。 蛋白质可以是全长或蛋白质片段。 IVT系统可以分批或连续模式使用。 GAMS可用作GamS核酸模板，粗蛋白级分或纯化蛋白质产物。 使用GamS组件的IVT系统可以以高通量模式使用。 还提供了基于从高通量小规模IVT表达产物获得的结果来预测可表达蛋白质或片段的能力以及大规模蛋白质表达产物的活性和溶解度的能力。