基本信息:
- 专利标题： HTLV-I TAX INDUCED KILLING OF p53 NULL CANCER CELLS
- 专利标题（中）：HTLV-I TAX诱导的p53空位癌细胞的杀伤
- 申请号：PCT/US2003/015280 申请日：2003-05-16
- 公开(公告)号：WO2003097799A2 公开(公告)日：2003-11-27
- 发明人： SEMMES, Oliver, John, IV , KUPFER, Gary, Michael
- 申请人： EASTERN VIRGINIA MEDICAL SCHOOL , SEMMES, Oliver, John, IV , KUPFER, Gary, Michael
- 申请人地址： 825 Fairfax Avenue, P.O. Box 1980, Norfolk, VA 23501-6006 US
- 专利权人： EASTERN VIRGINIA MEDICAL SCHOOL,SEMMES, Oliver, John, IV,KUPFER, Gary, Michael
- 当前专利权人： EASTERN VIRGINIA MEDICAL SCHOOL,SEMMES, Oliver, John, IV,KUPFER, Gary, Michael
- 当前专利权人地址： 825 Fairfax Avenue, P.O. Box 1980, Norfolk, VA 23501-6006 US
- 代理机构： MAEBIUS, Maria, L.
- 优先权： US60/380,853 20020517
- 主分类号： C12N
- IPC分类号： C12N
摘要：
This invention is based on the finding that expression of an exogenous nucleic acid encoding a polypeptide having human T-cell leukemia virus type I (HTLV-1) Tax activity in p53 null cells results in a sensitization of those cells to DNA damaging agents. Therefore, the present invention is directed to a method of inducing cell death in p53 null cells, enhancing susceptibility to such DNA damaging agents, and the selective cell killing of p53 null cells. It has been found that retroviral vectors, and particularly lentiviral vectors, are suitable for the present invention to permit the transient expression of the HTLV-1 Tax protein in the p53 null cells, which sensitizes the cells.
摘要（中）：
本发明基于以下发现：编码在p53无细胞中具有人T细胞白血病病毒I型（HTLV-1）Tax活性的多肽的外源核酸的表达导致这些细胞对DNA损伤剂的敏化。 因此，本发明涉及在p53无效细胞中诱导细胞死亡，增强对这种DNA损伤剂的易感性，以及p53无效细胞的选择性细胞杀伤的方法。 已经发现逆转录病毒载体，特别是慢病毒载体，适用于本发明，以允许HTLV-1 Tax蛋白在p53无效细胞中的瞬时表达，其使细胞敏感。
公开/授权文献:
- WO2003097799A3 HTLV-I TAX INDUCED KILLING OF p53 NULL CANCER CELLS 公开/授权日：2003-11-27